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    LIQUID AND GEL ELECTRODES FOR TRANSVERSE FREE FLOW ELECTROPHORESIS
    1.
    发明申请
    LIQUID AND GEL ELECTRODES FOR TRANSVERSE FREE FLOW ELECTROPHORESIS 有权
    液体和凝胶电极用于横向自由流动电泳

    公开(公告)号：US20120228141A1

    公开(公告)日：2012-09-13

    申请号：US13413161

    申请日：2012-03-06

    申请人： Byoungsok Jung ,  Klint A. Rose ,  Maxim Shusteff ,  Alexandre Persat ,  Juan Santiago

    发明人： Byoungsok Jung ,  Klint A. Rose ,  Maxim Shusteff ,  Alexandre Persat ,  Juan Santiago

    IPC分类号： C25B9/00 ,  B01D43/00

    CPC分类号： B01D43/00 ,  B01D57/02 ,  G01N27/44769

    摘要： The present invention provides a mechanism for separating or isolating charged particles under the influence of an electric field without metal electrodes being in direct contact with the sample solution. The metal electrodes normally in contact with the sample are replaced with high conductivity fluid electrodes situated parallel and adjacent to the sample. When the fluid electrodes transmit the electric field across the sample, particles within the sample migrate according to their electrophoretic mobility.

    摘要翻译： 本发明提供了一种用于在没有金属电极与样品溶液直接接触的电场的影响下分离或分离带电粒子的机理。 通常与样品接触的金属电极被替换为与样品平行且相邻的高导电性流体电极。 当流体电极跨越样品传播电场时，样品内的颗粒根据其电泳迁移率而迁移。

    METHODS FOR SEPARATING PARTICLES AND/OR NUCLEIC ACIDS USING ISOTACHOPHORESIS
    5.
    发明申请
    METHODS FOR SEPARATING PARTICLES AND/OR NUCLEIC ACIDS USING ISOTACHOPHORESIS 有权
    使用异烟肼分离颗粒和/或核酸的方法

    公开(公告)号：US20110036718A1

    公开(公告)日：2011-02-17

    申请号：US12542215

    申请日：2009-08-17

    申请人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

    发明人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44747 ,  G01N27/44773 ,  G01N2030/8827

    摘要： According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

    摘要翻译： 根据一个实施方案，一种方法包括将包含前导电解质，尾电解质和至少一种DNA和RNA的流体的流体共同输送到通道，以及在垂直于 诱导横向等电泳的通道。 在另一个实施例中，一种方法包括将流体共同输送到通道。 流体包括前导电解质，尾电解质，生物物体，DNA和RNA中的至少一种以及具有电泳迁移率的间隔电解质，所述间隔电解质位于至少一些生物体的电泳迁移率与电泳迁移率之间 至少一个DNA和RNA。 该方法还包括在垂直于通道的轴线的方向上向流体施加电场以诱导横向等速电泳。 除此之外还公开了其他的等速电泳方法。

    Methods for separating particles and/or nucleic acids using isotachophoresis
    6.
    发明授权
    Methods for separating particles and/or nucleic acids using isotachophoresis 有权
    使用等速电泳法分离颗粒和/或核酸的方法

    公开(公告)号：US09285340B2

    公开(公告)日：2016-03-15

    申请号：US12542215

    申请日：2009-08-17

    申请人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

    发明人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

    IPC分类号： G01N27/447

    CPC分类号： G01N27/44747 ,  G01N27/44773 ,  G01N2030/8827

    摘要： According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

    摘要翻译： 根据一个实施方案，一种方法包括将包含前导电解质，尾电解质和至少一种DNA和RNA的流体的流体共同输送到通道，以及在垂直于 诱导横向等电泳的通道。 在另一个实施例中，一种方法包括将流体共同供给到通道。 流体包括前导电解质，尾电解质，生物物体，DNA和RNA中的至少一种以及具有电泳迁移率的间隔电解质，所述间隔电解质位于至少一些生物体的电泳迁移率和电泳迁移率之间 至少一个DNA和RNA。 该方法还包括在垂直于通道的轴线的方向上向流体施加电场以诱导横向等速电泳。 除此之外还公开了其他的等速电泳法。

    Microfluidic electrophoresis chip having flow-retarding structure
    7.
    发明申请
    Microfluidic electrophoresis chip having flow-retarding structure 审中-公开
    具有阻流结构的微流控电泳芯片

    公开(公告)号：US20060042948A1

    公开(公告)日：2006-03-02

    申请号：US10932078

    申请日：2004-09-02

    申请人： Juan Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj

    发明人： Juan Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj

    IPC分类号： C07K1/26 ,  G01N27/447

    CPC分类号： G01N27/44791

    摘要： A capillary electrophoresis device and separation protocol uses a hydraulic resistance-providing structure (HRPS) in the main separation channel to separate the divide the main separate channel into an upstream portion and a downstream portion. The HRPS may take the form of a porous plug, or a solid plug provided with at least one shallow channel. A sample separates and migrates through the porous structure or the shallow channel, upon application of a voltage difference between the upstream and downstream sides. Among other things, the HRPS helps reduce electrokinetic flow in the presence of conductivity gradients and facilitates robust, high-gradient on-chip field amplified sample stacking. The HRPS also enables the use of a pressure-injection scheme for the introduction of a high conductivity gradient in a separation channel and thereby avoids flow instabilities associated with high conductivity gradient electrokinetics. The approach also allows for the suppression of electroosmotic flow (EOF) and benefits from the associated minimization of sample dispersion caused by non-uniform EOF mobilities. An injection procedure employing a single pressure-flow high-conductivity buffer injection step followed by standard high voltage control of electrophoretic fluxes of sample, may be employed.

    摘要翻译： 毛细管电泳装置和分离方案在主分离通道中使用液压阻力提供结构（HRPS），以将主分离通道分成上游部分和下游部分。 HRPS可以采用多孔插塞的形式，或者具有至少一个浅沟道的实心插头。 当上游侧和下游侧之间施加电压差时，样品分离并迁移通过多孔结构或浅沟道。 除了别的以外，HRPS有助于在电导率梯度的存在下减少电动流动，并且有助于稳健的高梯度片上场扩增样品堆叠。 HRPS还能够使用压力注入方案来在分离通道中引入高电导率梯度，从而避免与高电导率梯度电动力相关联的流动不稳定性。 该方法还允许抑制电渗流（EOF），并从由非均匀EOF迁移率引起的样品分散的相关最小化中获益。 可以采用使用单一压力 - 流动高电导率缓冲液注入步骤的注射程序，然后进行样品电泳通量的标准高压控制。

    Electrophoretic sample analysis and approach therefor
    8.
    发明授权
    Electrophoretic sample analysis and approach therefor 有权
    电泳样品分析及方法

    公开(公告)号：US08414754B1

    公开(公告)日：2013-04-09

    申请号：US11755449

    申请日：2007-05-30

    申请人： Juan G. Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj ,  Tarun Kumar Khurana

    发明人： Juan G. Santiago ,  Byoungsok Jung ,  Rajiv Bharadwaj ,  Tarun Kumar Khurana

    IPC分类号： B01D57/02 ,  G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44769

    摘要： Analysis of samples is facilitated. According to an example embodiment, an electrophoresis approach involves electrophoretically stacking and/or separating a sample or samples. An electrolyte and a mixture of one or more samples with another electrolyte are added to a microchannel or capillary. An electric field is applied to stack (and, in some applications, further separate) the one or more samples. Generally, the electric field and electrolyte are used to facilitate isotachophoretic (ITP) stacking. In some embodiments, a further electric field is applied and used with the electrolyte to facilitate subsequent capillary electrophoresis (CE).

    摘要翻译： 样品分析方便。 根据示例性实施方案，电泳方法涉及电泳堆叠和/或分离样品或样品。 将电解质和一种或多种样品与另一种电解质的混合物加入到微通道或毛细管中。 将一个或多个样品施加到堆叠（并且在一些应用中进一步分离）的电场。 通常，电场和电解质用于促进异羟基磷灰石（ITP）堆叠。 在一些实施方案中，施加另外的电场并与电解质一起使用以促进随后的毛细管电泳（CE）。