公开(公告)号：US09285340B2

公开(公告)日：2016-03-15

申请号：US12542215

申请日：2009-08-17

申请人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

发明人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

IPC分类号： G01N27/447

CPC分类号： G01N27/44747 ,  G01N27/44773 ,  G01N2030/8827

摘要： According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

摘要翻译： 根据一个实施方案，一种方法包括将包含前导电解质，尾电解质和至少一种DNA和RNA的流体的流体共同输送到通道，以及在垂直于 诱导横向等电泳的通道。 在另一个实施例中，一种方法包括将流体共同供给到通道。 流体包括前导电解质，尾电解质，生物物体，DNA和RNA中的至少一种以及具有电泳迁移率的间隔电解质，所述间隔电解质位于至少一些生物体的电泳迁移率和电泳迁移率之间 至少一个DNA和RNA。 该方法还包括在垂直于通道的轴线的方向上向流体施加电场以诱导横向等速电泳。 除此之外还公开了其他的等速电泳法。

公开(公告)号：US20110036718A1

公开(公告)日：2011-02-17

申请号：US12542215

申请日：2009-08-17

申请人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

发明人： Byoungsok Jung ,  Kevin Ness ,  Klint A. Rose

IPC分类号： G01N27/447

CPC分类号： G01N27/44747 ,  G01N27/44773 ,  G01N2030/8827

摘要： According to one embodiment, a method includes co-feeding fluids comprising a leading electrolyte, a trailing electrolyte, and at least one of DNA and RNA to a channel, and applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. In another embodiment, a method includes co-feeding fluids to a channel. The fluids include a leading electrolyte, a trailing electrolyte, biological objects, at least one of DNA and RNA, and a spacer electrolyte having an electrophoretic mobility that is between an electrophoretic mobility of at least some of the biological objects and an electrophoretic mobility of the at least one of the DNA and the RNA. The method also includes applying an electric field to the fluids in a direction perpendicular to an axis of the channel for inducing transverse isotachophoresis. Other methods of isotachophoresis are disclosed in addition to these.

摘要翻译： 根据一个实施方案，一种方法包括将包含前导电解质，尾电解质和至少一种DNA和RNA的流体的流体共同输送到通道，以及在垂直于 诱导横向等电泳的通道。 在另一个实施例中，一种方法包括将流体共同输送到通道。 流体包括前导电解质，尾电解质，生物物体，DNA和RNA中的至少一种以及具有电泳迁移率的间隔电解质，所述间隔电解质位于至少一些生物体的电泳迁移率与电泳迁移率之间 至少一个DNA和RNA。 该方法还包括在垂直于通道的轴线的方向上向流体施加电场以诱导横向等速电泳。 除此之外还公开了其他的等速电泳方法。

公开(公告)号：US08999129B2

公开(公告)日：2015-04-07

申请号：US13413161

申请日：2012-03-06

申请人： Byoungsok Jung ,  Klint A. Rose ,  Maxim Shusteff ,  Alexandre Persat ,  Juan Santiago

发明人： Byoungsok Jung ,  Klint A. Rose ,  Maxim Shusteff ,  Alexandre Persat ,  Juan Santiago

IPC分类号： G01N27/447 ,  B01D57/02 ,  B01D43/00

CPC分类号： B01D43/00 ,  B01D57/02 ,  G01N27/44769

摘要： The present invention provides a mechanism for separating or isolating charged particles under the influence of an electric field without metal electrodes being in direct contact with the sample solution. The metal electrodes normally in contact with the sample are replaced with high conductivity fluid electrodes situated parallel and adjacent to the sample. When the fluid electrodes transmit the electric field across the sample, particles within the sample migrate according to their electrophoretic mobility.

摘要翻译： 本发明提供了一种用于在没有金属电极与样品溶液直接接触的电场的影响下分离或分离带电粒子的机理。 通常与样品接触的金属电极被替换为与样品平行且相邻的高导电性流体电极。 当流体电极跨越样品传播电场时，样品内的颗粒根据其电泳迁移率而迁移。

公开(公告)号：US20130043170A1

公开(公告)日：2013-02-21

申请号：US13571640

申请日：2012-08-10

申请人： Klint A. Rose ,  Karl A. Fisher ,  Douglas A. Wajda ,  Raymond P. Mariella, JR. ,  Christopher Bailey ,  Dietrich Dehlinger ,  Maxim Shusteff ,  Byoungsok Jung ,  Kevin D. Ness

发明人： Klint A. Rose ,  Karl A. Fisher ,  Douglas A. Wajda ,  Raymond P. Mariella, JR. ,  Christopher Bailey ,  Dietrich Dehlinger ,  Maxim Shusteff ,  Byoungsok Jung ,  Kevin D. Ness

IPC分类号： B03B5/60

CPC分类号： B01L3/50273 ,  B01D21/28 ,  B01D21/283 ,  B01D2221/10 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0439 ,  B01L2400/0487 ,  G01N1/4077 ,  G01N33/48728 ,  G01N33/491 ,  G01N2001/4094

摘要： An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.

摘要翻译： 超声波微流体系统包括用于输送包含小颗粒和大颗粒的样品流体的分离通道，所述样品流体基本上平行于与其接触的回收流体相邻流动。 声换能器产生超声驻波，其产生具有至少一个最小压力振幅节点的压力场。 声学延伸结构位于所述分离通道附近，用于将所述声音节点偏离所述声学区域的中心，并将大颗粒集中在所述回收流体流中。

公开(公告)号：US08727129B2

公开(公告)日：2014-05-20

申请号：US13571640

申请日：2012-08-10

申请人： Klint A. Rose ,  Karl A. Fisher ,  Douglas A. Wajda ,  Raymond P. Mariella, Jr. ,  Christopher Bailey ,  Dietrich Dehlinger ,  Maxim Shusteff ,  Byoungsok Jung ,  Kevin D. Ness

发明人： Klint A. Rose ,  Karl A. Fisher ,  Douglas A. Wajda ,  Raymond P. Mariella, Jr. ,  Christopher Bailey ,  Dietrich Dehlinger ,  Maxim Shusteff ,  Byoungsok Jung ,  Kevin D. Ness

IPC分类号： B03B5/00

CPC分类号： B01L3/50273 ,  B01D21/28 ,  B01D21/283 ,  B01D2221/10 ,  B01L3/502761 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0439 ,  B01L2400/0487 ,  G01N1/4077 ,  G01N33/48728 ,  G01N33/491 ,  G01N2001/4094

摘要： An ultrasonic microfluidic system includes a separation channel for conveying a sample fluid containing small particles and large particles, flowing substantially parallel, adjacent to a recovery fluid, with which it is in contact. An acoustic transducer produces an ultrasound standing wave, that generates a pressure field having at least one node of minimum pressure amplitude. An acoustic extension structure is located proximate to said separation channel for positioning said acoustic node off center in said acoustic area and concentrating the large particles in said recovery fluid stream.

摘要翻译： 超声波微流体系统包括用于输送包含小颗粒和大颗粒的样品流体的分离通道，所述样品流体基本上平行于与其接触的回收流体相邻流动。 声换能器产生超声驻波，其产生具有至少一个最小压力振幅节点的压力场。 声学延伸结构位于所述分离通道附近，用于将所述声音节点偏离所述声学区域的中心，并将大颗粒集中在所述回收流体流中。

公开(公告)号：US20120228141A1

公开(公告)日：2012-09-13

申请号：US13413161

申请日：2012-03-06

申请人： Byoungsok Jung ,  Klint A. Rose ,  Maxim Shusteff ,  Alexandre Persat ,  Juan Santiago

发明人： Byoungsok Jung ,  Klint A. Rose ,  Maxim Shusteff ,  Alexandre Persat ,  Juan Santiago

IPC分类号： C25B9/00 ,  B01D43/00

CPC分类号： B01D43/00 ,  B01D57/02 ,  G01N27/44769

摘要： The present invention provides a mechanism for separating or isolating charged particles under the influence of an electric field without metal electrodes being in direct contact with the sample solution. The metal electrodes normally in contact with the sample are replaced with high conductivity fluid electrodes situated parallel and adjacent to the sample. When the fluid electrodes transmit the electric field across the sample, particles within the sample migrate according to their electrophoretic mobility.

摘要翻译： 本发明提供了一种用于在没有金属电极与样品溶液直接接触的电场的影响下分离或分离带电粒子的机理。 通常与样品接触的金属电极被替换为与样品平行且相邻的高导电性流体电极。 当流体电极跨越样品传播电场时，样品内的颗粒根据其电泳迁移率而迁移。

公开(公告)号：US08524064B2

公开(公告)日：2013-09-03

申请号：US12843269

申请日：2010-07-26

申请人： Dietrich A. Dehlinger ,  Klint A. Rose ,  Maxim Shusteff ,  Christopher G. Bailey ,  Raymond P. Mariella, Jr.

发明人： Dietrich A. Dehlinger ,  Klint A. Rose ,  Maxim Shusteff ,  Christopher G. Bailey ,  Raymond P. Mariella, Jr.

IPC分类号： G01N27/26 ,  G01N27/453 ,  B03C5/02 ,  B01D57/02

CPC分类号： B03C5/005 ,  B01L2400/0424 ,  B03C5/026 ,  G01N27/447

摘要： A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.

摘要翻译： 一种用于从样品中分离颗粒的介电电泳装置，包括装置体; 所述设备主体中的介电电泳通道，所述介电电泳通道具有中心轴线，底部，顶部，第一侧面和第二侧面; 第一台面从底部突出到介电电泳通道中并且从第一侧穿过介电电泳通道延伸到第二侧，第一台面以与介电电泳通道的中心轴线成一定角度延伸; 沿第一台面延伸的第一电极; 第二台面从底部突出到介电电泳通道中，并从第一侧穿过介电电泳通道延伸到第二侧，第二台面以与介电电泳通道的中心轴成一定角度延伸; 第一电极和第二电极和第二电极和第二侧中的至少一个之间的空间; 以及第一电极和第二电极之间的间隙。

公开(公告)号：US07030411B2

公开(公告)日：2006-04-18

申请号：US10939771

申请日：2004-09-13

申请人： Peter A. Krulevitch ,  Mariam N. Maghribi ,  William J. Benett ,  Julie K. Hamilton ,  Klint A. Rose ,  James Courtney Davidson ,  Mark S. Strauch

发明人： Peter A. Krulevitch ,  Mariam N. Maghribi ,  William J. Benett ,  Julie K. Hamilton ,  Klint A. Rose ,  James Courtney Davidson ,  Mark S. Strauch

IPC分类号： H01L29/04 ,  H01L31/20 ,  H01L31/036 ,  H01L31/0376

CPC分类号： A61N1/0456 ,  A61B2560/0412 ,  A61N1/0452 ,  A61N1/0476 ,  A61N1/0492 ,  A61N1/05 ,  A61N1/0534 ,  H01L23/5387 ,  H01L25/065 ,  H01L2924/0002 ,  H01L2924/09701 ,  H05K1/0313 ,  H05K1/0393 ,  H05K2201/0162 ,  H05K2201/09118 ,  H01L2924/00

摘要： A peel and stick electronic system comprises a silicone body, and at least one electronic unit operatively connected to the silicone body. The electronic system is produce by providing a silicone layer on a substrate, providing a metal layer on the silicone layer, and providing at least one electronic unit connected to the metal layer.

摘要翻译： 剥离和粘贴电子系统包括硅树脂体和至少一个可操作地连接到硅氧烷体的电子单元。 该电子系统是通过在衬底上提供硅树脂层，在硅树脂层上提供金属层，并提供至少一个与金属层连接的电子单元来制造的。

公开(公告)号：US08968865B2

公开(公告)日：2015-03-03

申请号：US13171323

申请日：2011-06-28

申请人： Marcus A. Worsley ,  Theodore F. Baumann ,  Joe H. Satcher ,  Tammy Y. Olson ,  Joshua D. Kuntz ,  Klint A. Rose

发明人： Marcus A. Worsley ,  Theodore F. Baumann ,  Joe H. Satcher ,  Tammy Y. Olson ,  Joshua D. Kuntz ,  Klint A. Rose

IPC分类号： B32B3/26 ,  B32B5/14 ,  C25D13/02 ,  C25D15/00 ,  C25D13/22 ,  C25D1/00

CPC分类号： C25D13/02 ,  C25D1/006 ,  C25D13/22 ,  C25D15/00 ,  Y10T428/249921 ,  Y10T428/249961 ,  Y10T428/249999 ,  Y10T428/254

摘要： In one embodiment, an aerogel includes a layer of shaped particles having a particle packing density gradient in a thickness direction of the layer, wherein the shaped particles are characterized by being formed in an electrophoretic deposition (EPD) process using an impurity. In another embodiment, a method for forming a functionally graded porous nanostructure includes adding particles of an impurity and a solution to an EPD chamber, applying a voltage difference across the two electrodes of the EPD chamber to create an electric field in the EPD chamber, and depositing the material onto surfaces of the particles of the impurity to form shaped particles of the material. Other functionally graded materials and methods are described according to more embodiments.

摘要翻译： 在一个实施方案中，气凝胶包括在层的厚度方向上具有颗粒填充密度梯度的成形颗粒层，其中成型颗粒的特征在于使用杂质在电泳沉积（EPD）方法中形成。 在另一个实施方案中，用于形成功能梯度的多孔纳米结构的方法包括将杂质颗粒和溶液添加到EPD室中，在EPD室的两个电极之间施加电压差以在EPD室中产生电场，以及 将材料沉积在杂质颗粒的表面上以形成材料的成形颗粒。 根据更多实施例描述了其它功能梯度材料和方法。

公开(公告)号：US20130306481A1

公开(公告)日：2013-11-21

申请号：US13945768

申请日：2013-07-18

申请人： Dietrich A. Dehlinger ,  Klint A. Rose ,  Maxim Shusteff ,  Christopher G. Bailey ,  Raymond P. Mariella, JR.

发明人： Dietrich A. Dehlinger ,  Klint A. Rose ,  Maxim Shusteff ,  Christopher G. Bailey ,  Raymond P. Mariella, JR.

IPC分类号： B03C5/00

CPC分类号： B03C5/005 ,  B01L2400/0424 ,  B03C5/026 ,  G01N27/447

摘要： A dielectrophoresis apparatus for separating particles from a sample, including an apparatus body; a dielectrophoresis channel in the apparatus body, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a first electrode extending along the first mesa; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa extending at an angle to the central axis of the dielectrophoresis channel; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode.

摘要翻译： 一种用于从样品中分离颗粒的介电电泳装置，包括装置体; 所述设备主体中的介电电泳通道，所述介电电泳通道具有中心轴线，底部，顶部，第一侧面和第二侧面; 第一台面从底部突出到介电电泳通道中并且从第一侧穿过介电电泳通道延伸到第二侧，第一台面以与介电电泳通道的中心轴线成一定角度延伸; 沿第一台面延伸的第一电极; 从底部突出到介电电泳通道的第二台面，并且从第一侧穿过介电电泳通道延伸到第二侧，第二台面以与介电电泳通道的中心轴线成一定角度延伸; 第一电极和第二电极和第二电极和第二侧中的至少一个之间的空间; 以及第一电极和第二电极之间的间隙。