公开(公告)号：US20180193839A1

公开(公告)日：2018-07-12

申请号：US15863330

申请日：2018-01-05

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Sami Kanderian ,  Gregory H. Owen ,  Hongye Liang ,  Scott Corey ,  Brian Bean

IPC: B01L3/00 ,  G01N33/58

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2400/082 ,  G01N33/582 ,  Y10T137/0318

Abstract: The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.

公开(公告)号：US20160051985A1

公开(公告)日：2016-02-25

申请号：US14833939

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Ivor T. KNIGHT ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC: B01L3/00 ,  G01N21/77 ,  B01L7/00

CPC classification number: B01L3/502746 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/77 ,  G01N35/08 ,  G01N35/1095 ,  G01N2021/7786 ,  C12Q2561/113 ,  C12Q2565/629

Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

Abstract translation: 本发明涉及用于在聚合酶链反应（PCR）和热熔体应用中实时处理核酸的系统和方法。 根据本发明的一个方面，提供了用于多重核酸测定的快速连续处理的系统。 在一个实施例中，该系统包括但不限于：具有微流体（流通）通道的微流控筒，荧光成像系统，温度测量和控制系统; 压力测量和控制系统，用于将可变气动压力施加到微流控筒; 用于容纳多个试剂（例如，孔板）的储存装置; 液体处理系统，包括至少一个用于将试剂混合物抽吸，混合和分配到微流控盒的机器人移液器; 用于数据存储，处理和输出的系统; 和一个系统控制器来协调各种设备和功能。

公开(公告)号：US20140039802A1

公开(公告)日：2014-02-06

申请号：US13855329

申请日：2013-04-02

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Sami Kanderian

IPC: G06F19/22

CPC classification number: G16B30/00 ,  C12Q1/6827 ,  G16B40/00 ,  C12Q2527/101 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of determining whether a genotype is present in a biological sample. The present invention includes methods and systems for determining whether a genotype is present in a biological sample, through generating a dynamic profile an unknown genotype, correlating the dynamic profile to an average profile for a known genotype to generate a correlation value, and determining whether the correlation value falls within an acceptable threshold to determine if the unknown genotype is the known genotype. The present invention also relates to methods and systems for generating a training set to allow a machine to recognize a known genotype from within a class of known genotypes through generating dynamic profiles of a known genotype, averaging the dynamic profiles to generate an average profile for the genotype, and correlating the dynamic profiles of the genotype with an average profile for each known genotype in a class of genotypes to generate a correlation vector. The training set generated by these methods and systems may be used to assist in identification of unknown genotypes.

Abstract translation: 本发明涉及用于分析核酸解离行为的方法和系统以及确定基因型是否存在于生物样品中的鉴定。 本发明包括用于通过生成未知基因型的动态分布来确定生物样品中是否存在基因型的方法和系统，将动态分布与已知基因型的平均分布相关联以产生相关值，以及确定是否 相关值落在可接受的阈值内，以确定未知的基因型是否是已知的基因型。 本发明还涉及用于生成训练集的方法和系统，以允许机器通过生成已知基因型的动态分布来识别来自一类已知基因型的已知基因型，对动态特征进行平均以生成平均轮廓 基因型，并将基因型的动态特征与一类基因型中的每种已知基因型的平均轮廓相关联以产生相关性载体。 由这些方法和系统产生的训练集可用于帮助识别未知的基因型。

公开(公告)号：US20140107937A1

公开(公告)日：2014-04-17

申请号：US13937522

申请日：2013-07-09

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Sami Kanderian

IPC: G06F19/18

CPC classification number: G16B20/00 ,  C12Q1/6827 ,  G16B40/00 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids. The present invention includes methods and systems for analyzing dynamic profiles of genotypes of nucleic acids, including the steps of using a computer, including a processor and a memory, to convert dynamic profiles of known genotypes of a nucleic acid to multi-dimensional data points, wherein the dynamic profiles each comprise measurements of a signal representing a physical change of a nucleic acid containing the known genotype relative to an independent variable; using the computer to reduce the multi-dimensional data points into reduced-dimensional data points; and generating a plot of the reduced-dimensional data points for each genotype. The present invention also relates to methods and systems for calculating error statistics for an assay for identifying a genotype in a biological sample using an enhanced Monte Carlo simulation method to generate a set of N random data points for each known genotype within a class of known genotypes, where each set of N random data points has the same mean data point and covariance matrix as a data set for each of the known genotypes.

Abstract translation: 本发明涉及用于分析核酸解离行为的方法和系统。 本发明包括用于分析核酸基因型的动态谱的方法和系统，包括使用包括处理器和存储器的计算机将核酸的已知基因型的动态谱转化为多维数据点的步骤， 其中所述动态分布图包括相对于独立变量表示含有已知基因型的核酸的物理变化的信号的测量值; 使用计算机将多维数据点减少为维数据点; 并且生成针对每个基因型的缩小维数据点的图。 本发明还涉及用于使用增强型蒙特卡罗模拟方法来计算生物样品中基因型的测定的误差统计的方法和系统，以生成一组已知基因型内的每个已知基因型的一组N个随机数据点 ，其中每组N个随机数据点具有与每个已知基因型的数据集相同的平均数据点和协方差矩阵。

公开(公告)号：US10363558B2

公开(公告)日：2019-07-30

申请号：US14833939

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Ivor T. Knight ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC: B01L3/00 ,  B01L7/00 ,  G01N21/77 ,  C12Q1/686 ,  G01N35/08 ,  G01N35/10

Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

公开(公告)号：US09292653B2

公开(公告)日：2016-03-22

申请号：US14101647

申请日：2013-12-10

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Deborah Boles ,  Kenton C. Hasson ,  Sami Kanderian

IPC: G06F19/24 ,  G06F19/20 ,  C12Q1/68 ,  B01L7/00 ,  G06F19/18

CPC classification number: G06F19/24 ,  B01L7/52 ,  C12Q1/6816 ,  G06F19/18 ,  G06F19/20 ,  C12Q2527/107 ,  C12Q2537/165 ,  C12Q2539/101

Abstract: The present invention relates to methods and systems for the analysis of the dissociation behavior of nucleic acids and the identification of nucleic acids. In one aspect, methods and systems are disclosed for identifying a nucleic acid in a sample including an unknown nucleic acid and for detecting a single nucleotide polymorphism in a nucleic acid in a sample. In another aspect, methods and systems are disclosed for identification of a nucleic acid in a biological sample including at least one unknown nucleic acid by fitting denaturation data including measurements of a quantifiable physical change of the sample at a plurality of independent sample property points to a function to determine an intrinsic physical value and to obtain an estimated physical change function, and identifying the nucleic acid in the biological sample by comparing the intrinsic physical value for at least one unknown nucleic acid to an intrinsic physical value for a known nucleic acid.

Abstract translation: 本发明涉及用于分析核酸的解离行为和核酸鉴定的方法和系统。 在一个方面，公开了用于鉴定样品中的核酸的方法和系统，所述样品包括未知核酸并用于检测样品中核酸中的单核苷酸多态性。 在另一方面，公开了用于通过拟合变性数据来包括至少一种未知核酸的生物样品中的核酸的鉴定的方法和系统，所述变性数据包括在多个独立样本特性点处的样品的可量化物理变化的测量值 用于确定固有物理值并获得估计的物理变化功能，以及通过将至少一种未知核酸的固有物理值与已知核酸的固有物理值进行比较来鉴定生物样品中的核酸。