公开(公告)号：US20160051985A1

公开(公告)日：2016-02-25

申请号：US14833939

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Ivor T. KNIGHT ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC: B01L3/00 ,  G01N21/77 ,  B01L7/00

CPC classification number: B01L3/502746 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/77 ,  G01N35/08 ,  G01N35/1095 ,  G01N2021/7786 ,  C12Q2561/113 ,  C12Q2565/629

Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

Abstract translation: 本发明涉及用于在聚合酶链反应（PCR）和热熔体应用中实时处理核酸的系统和方法。 根据本发明的一个方面，提供了用于多重核酸测定的快速连续处理的系统。 在一个实施例中，该系统包括但不限于：具有微流体（流通）通道的微流控筒，荧光成像系统，温度测量和控制系统; 压力测量和控制系统，用于将可变气动压力施加到微流控筒; 用于容纳多个试剂（例如，孔板）的储存装置; 液体处理系统，包括至少一个用于将试剂混合物抽吸，混合和分配到微流控盒的机器人移液器; 用于数据存储，处理和输出的系统; 和一个系统控制器来协调各种设备和功能。

公开(公告)号：US08992860B2

公开(公告)日：2015-03-31

申请号：US14252234

申请日：2014-04-14

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Brian Murphy ,  Scott Corey ,  Alex Flamm ,  Ben Lane ,  Conrad Laskowski ,  Chad Schneider

IPC: B01L3/00 ,  C12Q1/68 ,  B01L7/00 ,  G01N33/50 ,  G01N21/66 ,  F04B49/10 ,  H05B1/02 ,  F04B49/06 ,  G01N35/00

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/18 ,  C12Q2565/629 ,  F04B49/065 ,  F04B49/106 ,  G01N21/66 ,  G01N33/50 ,  G01N2035/00544 ,  H05B1/025 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/25

Abstract: The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.

Abstract translation: 本发明涉及用于最小化或消除扩散效应的系统和方法。 多个可混溶流体物质的分段流的扩散区域可以排放到废物通道，并且流体的非扩散区域可以优先地从包含分段流动的通道中拉出。 可以收集未经扩散污染的多个流体样品，以便在单个通道中进行分析和测量。 可以使用用于最小化或消除扩散效应的系统和方法来最小化或消除用于监测DNA分子的扩增和DNA分子的解离行为的微流体系统中的扩散效应。

公开(公告)号：US20140220584A1

公开(公告)日：2014-08-07

申请号：US14252234

申请日：2014-04-14

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Brian Murphy ,  Scott Corey ,  Alex Flamm ,  Ben Lane ,  Conrad Laskowski ,  Chad Schneider

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/18 ,  C12Q2565/629 ,  F04B49/065 ,  F04B49/106 ,  G01N21/66 ,  G01N33/50 ,  G01N2035/00544 ,  H05B1/025 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/25

Abstract: The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.

Abstract translation: 本发明涉及用于最小化或消除扩散效应的系统和方法。 多个可混溶流体物质的分段流的扩散区域可以排出到废物通道，并且流体的非扩散区域可以优先地从包含分段流动的通道中拉出。 可以收集未经扩散污染的多个流体样品，以便在单个通道中进行分析和测量。 可以使用用于最小化或消除扩散效应的系统和方法来最小化或消除用于监测DNA分子的扩增和DNA分子的解离行为的微流体系统中的扩散效应。

公开(公告)号：US10363558B2

公开(公告)日：2019-07-30

申请号：US14833939

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Ivor T. Knight ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC: B01L3/00 ,  B01L7/00 ,  G01N21/77 ,  C12Q1/686 ,  G01N35/08 ,  G01N35/10

Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

公开(公告)号：US09919314B2

公开(公告)日：2018-03-20

申请号：US14923058

申请日：2015-10-26

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Ben Lane ,  Eric Schneider

IPC: B01L3/00 ,  B01L7/00 ,  F28F9/02 ,  C12M1/40 ,  C12P19/34 ,  G01N35/08 ,  C07H1/06 ,  C07H21/04 ,  G01N35/00

CPC classification number: B01L7/52 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/147 ,  B01L2300/041 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1805 ,  B01L2300/1838 ,  B01L2300/1844 ,  B01L2300/1883 ,  B01L2300/1894 ,  C12P19/34 ,  F28F9/02 ,  G01N35/08 ,  G01N2035/00346 ,  Y02P20/129

Abstract: Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.

公开(公告)号：US20160038945A1

公开(公告)日：2016-02-11

申请号：US14923058

申请日：2015-10-26

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Ben Lane ,  Eric Schneider

IPC: B01L7/00 ,  F28F9/02 ,  C12P19/34

CPC classification number: B01L7/52 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/147 ,  B01L2300/041 ,  B01L2300/06 ,  B01L2300/18 ,  B01L2300/1805 ,  B01L2300/1838 ,  B01L2300/1844 ,  B01L2300/1883 ,  B01L2300/1894 ,  C12P19/34 ,  F28F9/02 ,  G01N35/08 ,  G01N2035/00346 ,  Y02P20/129

Abstract: Systems and methods for air cooling a microfluidic device using confinement channels to isolate cooling air from exposed liquids are disclosed. The systems and methods may also thermally condition the cooling airflow for improved robustness of the microfluidic device. In one embodiment, the air cooling system includes a split-level cooling manifold including an inlet duct that directs cooling air to a microfluidic device and an outlet duct that directs air heated by the microfluidic device away from the microfluidic device. The temperature of cooling air may be measured. The cooling air may be preheated to a temperature that is higher than an expected ambient temperature. The temperature of the cooling air after being heated by a microfluidic device may be measured.

Abstract translation: 公开了使用限制通道空气冷却微流体装置以将冷却空气与暴露液体隔离的系统和方法。 该系统和方法还可以热调节冷却气流，以改善微流体装置的鲁棒性。 在一个实施例中，空气冷却系统包括分级冷却歧管，其包括将冷却空气引导到微流体装置的入口管道和将微流体装置加热的空气远离微流体装置的出口管道。 可以测量冷却空气的温度。 冷却空气可以被预热到比预期的环境温度高的温度。 可以测量由微流体装置加热后的冷却空气的温度。