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1.发明申请公开(公告)号：US20160051985A1
公开(公告)日：2016-02-25
申请号：US14833939
申请日：2015-08-24
发明人： Ivor T. KNIGHT , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
CPC分类号： B01L3/502746 , B01L3/502784 , B01L7/52 , B01L7/525 , B01L2200/025 , B01L2200/027 , B01L2200/028 , B01L2200/10 , B01L2300/0663 , B01L2300/0816 , B01L2300/1822 , B01L2300/1827 , B01L2300/1844 , B01L2400/0487 , C12Q1/686 , G01N21/77 , G01N35/08 , G01N35/1095 , G01N2021/7786 , C12Q2561/113 , C12Q2565/629
摘要： The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
摘要翻译： 本发明涉及用于在聚合酶链反应（PCR）和热熔体应用中实时处理核酸的系统和方法。 根据本发明的一个方面，提供了用于多重核酸测定的快速连续处理的系统。 在一个实施例中，该系统包括但不限于：具有微流体（流通）通道的微流控筒，荧光成像系统，温度测量和控制系统; 压力测量和控制系统，用于将可变气动压力施加到微流控筒; 用于容纳多个试剂（例如，孔板）的储存装置; 液体处理系统，包括至少一个用于将试剂混合物抽吸，混合和分配到微流控盒的机器人移液器; 用于数据存储，处理和输出的系统; 和一个系统控制器来协调各种设备和功能。
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2.发明授权Systems and methods for minimization or elimination of diffusion effects in a microfluidic system 有权用于在微流体系统中最小化或消除扩散效应的系统和方法公开(公告)号：US08992860B2
公开(公告)日：2015-03-31
申请号：US14252234
申请日：2014-04-14
发明人： Brian Murphy , Scott Corey , Alex Flamm , Ben Lane , Conrad Laskowski , Chad Schneider
IPC分类号： B01L3/00 , C12Q1/68 , B01L7/00 , G01N33/50 , G01N21/66 , F04B49/10 , H05B1/02 , F04B49/06 , G01N35/00
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502784 , B01L7/525 , B01L2200/0684 , B01L2200/10 , B01L2200/141 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/18 , C12Q2565/629 , F04B49/065 , F04B49/106 , G01N21/66 , G01N33/50 , G01N2035/00544 , H05B1/025 , Y10S435/808 , Y10S436/805 , Y10S436/809 , Y10T436/25
摘要： The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.
摘要翻译： 本发明涉及用于最小化或消除扩散效应的系统和方法。 多个可混溶流体物质的分段流的扩散区域可以排放到废物通道，并且流体的非扩散区域可以优先地从包含分段流动的通道中拉出。 可以收集未经扩散污染的多个流体样品，以便在单个通道中进行分析和测量。 可以使用用于最小化或消除扩散效应的系统和方法来最小化或消除用于监测DNA分子的扩增和DNA分子的解离行为的微流体系统中的扩散效应。
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3.发明申请SYSTEMS AND METHODS FOR MINIMIZATION OR ELIMINATION OF DIFFUSION EFFECTS IN A MICROFLUIDIC SYSTEM 有权微流控系统中微量化或消除扩散效应的系统和方法公开(公告)号：US20140220584A1
公开(公告)日：2014-08-07
申请号：US14252234
申请日：2014-04-14
发明人： Brian Murphy , Scott Corey , Alex Flamm , Ben Lane , Conrad Laskowski , Chad Schneider
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502784 , B01L7/525 , B01L2200/0684 , B01L2200/10 , B01L2200/141 , B01L2200/147 , B01L2300/0816 , B01L2300/0861 , B01L2300/0867 , B01L2300/18 , C12Q2565/629 , F04B49/065 , F04B49/106 , G01N21/66 , G01N33/50 , G01N2035/00544 , H05B1/025 , Y10S435/808 , Y10S436/805 , Y10S436/809 , Y10T436/25
摘要： The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.
摘要翻译： 本发明涉及用于最小化或消除扩散效应的系统和方法。 多个可混溶流体物质的分段流的扩散区域可以排出到废物通道，并且流体的非扩散区域可以优先地从包含分段流动的通道中拉出。 可以收集未经扩散污染的多个流体样品，以便在单个通道中进行分析和测量。 可以使用用于最小化或消除扩散效应的系统和方法来最小化或消除用于监测DNA分子的扩增和DNA分子的解离行为的微流体系统中的扩散效应。
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公开(公告)号：US10363558B2
公开(公告)日：2019-07-30
申请号：US14833939
申请日：2015-08-24
发明人： Ivor T. Knight , Kenton C. Hasson , Johnathan S. Coursey , Hongye Liang , Sami Kanderian , Gregory H. Owen , Weidong Cao , Ying-Xin Wang , Scott Corey , Ben Lane , Conrad Laskowski , Alex Flamm , Brian Murphy , Eric Schneider , Takayoshi Hanagata , Hiroshi Inoue , Shulin Zeng , Brian Bean , Franklin Regan
摘要： The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.
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