公开(公告)号：US20180193839A1

公开(公告)日：2018-07-12

申请号：US15863330

申请日：2018-01-05

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Sami Kanderian ,  Gregory H. Owen ,  Hongye Liang ,  Scott Corey ,  Brian Bean

IPC: B01L3/00 ,  G01N33/58

CPC classification number: B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/143 ,  B01L2200/147 ,  B01L2400/082 ,  G01N33/582 ,  Y10T137/0318

Abstract: The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs.

公开(公告)号：US20160051985A1

公开(公告)日：2016-02-25

申请号：US14833939

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Ivor T. KNIGHT ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC: B01L3/00 ,  G01N21/77 ,  B01L7/00

CPC classification number: B01L3/502746 ,  B01L3/502784 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/10 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2400/0487 ,  C12Q1/686 ,  G01N21/77 ,  G01N35/08 ,  G01N35/1095 ,  G01N2021/7786 ,  C12Q2561/113 ,  C12Q2565/629

Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

Abstract translation: 本发明涉及用于在聚合酶链反应（PCR）和热熔体应用中实时处理核酸的系统和方法。 根据本发明的一个方面，提供了用于多重核酸测定的快速连续处理的系统。 在一个实施例中，该系统包括但不限于：具有微流体（流通）通道的微流控筒，荧光成像系统，温度测量和控制系统; 压力测量和控制系统，用于将可变气动压力施加到微流控筒; 用于容纳多个试剂（例如，孔板）的储存装置; 液体处理系统，包括至少一个用于将试剂混合物抽吸，混合和分配到微流控盒的机器人移液器; 用于数据存储，处理和输出的系统; 和一个系统控制器来协调各种设备和功能。

公开(公告)号：US10363558B2

公开(公告)日：2019-07-30

申请号：US14833939

申请日：2015-08-24

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Ivor T. Knight ,  Kenton C. Hasson ,  Johnathan S. Coursey ,  Hongye Liang ,  Sami Kanderian ,  Gregory H. Owen ,  Weidong Cao ,  Ying-Xin Wang ,  Scott Corey ,  Ben Lane ,  Conrad Laskowski ,  Alex Flamm ,  Brian Murphy ,  Eric Schneider ,  Takayoshi Hanagata ,  Hiroshi Inoue ,  Shulin Zeng ,  Brian Bean ,  Franklin Regan

IPC: B01L3/00 ,  B01L7/00 ,  G01N21/77 ,  C12Q1/686 ,  G01N35/08 ,  G01N35/10

Abstract: The present invention relates to systems and methods for the real time processing of nucleic acid during polymerase chain reaction (PCR) and thermal melt applications. According to an aspect of the invention, a system for the rapid serial processing of multiple nucleic acid assays is provided. In one embodiment, the system includes, but is not limited to: a microfluidic cartridge having microfluidic (flow-through) channels, a fluorescence imaging system, a temperature measurement and control system; a pressure measurement and control system for applying variable pneumatic pressures to the microfluidic cartridge; a storage device for holding multiple reagents (e.g., a well-plate); a liquid handling system comprising at least one robotic pipettor for aspirating, mixing, and dispensing reagent mixtures to the microfluidic cartridge; systems for data storage, processing, and output; and a system controller to coordinate the various devices and functions.

公开(公告)号：US08992860B2

公开(公告)日：2015-03-31

申请号：US14252234

申请日：2014-04-14

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Brian Murphy ,  Scott Corey ,  Alex Flamm ,  Ben Lane ,  Conrad Laskowski ,  Chad Schneider

IPC: B01L3/00 ,  C12Q1/68 ,  B01L7/00 ,  G01N33/50 ,  G01N21/66 ,  F04B49/10 ,  H05B1/02 ,  F04B49/06 ,  G01N35/00

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/18 ,  C12Q2565/629 ,  F04B49/065 ,  F04B49/106 ,  G01N21/66 ,  G01N33/50 ,  G01N2035/00544 ,  H05B1/025 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/25

Abstract: The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.

Abstract translation: 本发明涉及用于最小化或消除扩散效应的系统和方法。 多个可混溶流体物质的分段流的扩散区域可以排放到废物通道，并且流体的非扩散区域可以优先地从包含分段流动的通道中拉出。 可以收集未经扩散污染的多个流体样品，以便在单个通道中进行分析和测量。 可以使用用于最小化或消除扩散效应的系统和方法来最小化或消除用于监测DNA分子的扩增和DNA分子的解离行为的微流体系统中的扩散效应。

公开(公告)号：US20140220584A1

公开(公告)日：2014-08-07

申请号：US14252234

申请日：2014-04-14

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Brian Murphy ,  Scott Corey ,  Alex Flamm ,  Ben Lane ,  Conrad Laskowski ,  Chad Schneider

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502715 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/18 ,  C12Q2565/629 ,  F04B49/065 ,  F04B49/106 ,  G01N21/66 ,  G01N33/50 ,  G01N2035/00544 ,  H05B1/025 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/809 ,  Y10T436/25

Abstract: The present invention relates to systems and methods for minimizing or eliminating diffusion effects. Diffused regions of a segmented flow of multiple, miscible fluid species may be vented off to a waste channel, and non-diffused regions of fluid may be preferentially pulled off the channel that contains the segmented flow. Multiple fluid samples that are not contaminated via diffusion may be collected for analysis and measurement in a single channel. The systems and methods for minimizing or eliminating diffusion effects may be used to minimize or eliminate diffusion effects in a microfluidic system for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules.

Abstract translation: 本发明涉及用于最小化或消除扩散效应的系统和方法。 多个可混溶流体物质的分段流的扩散区域可以排出到废物通道，并且流体的非扩散区域可以优先地从包含分段流动的通道中拉出。 可以收集未经扩散污染的多个流体样品，以便在单个通道中进行分析和测量。 可以使用用于最小化或消除扩散效应的系统和方法来最小化或消除用于监测DNA分子的扩增和DNA分子的解离行为的微流体系统中的扩散效应。

公开(公告)号：US20180179602A1

公开(公告)日：2018-06-28

申请号：US15905330

申请日：2018-02-26

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Brian Bean ,  Scott Corey

IPC: C12Q3/00 ,  C12Q1/686 ,  B01L7/00 ,  B01L3/00

CPC classification number: C12Q3/00 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0487 ,  C12Q1/686

Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.

公开(公告)号：US09903003B2

公开(公告)日：2018-02-27

申请号：US13838411

申请日：2013-03-15

Applicant: Canon U.S. Life Sciences, Inc.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Brian Bean ,  Scott Corey

IPC: C12Q1/68 ,  C12Q3/00 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q3/00 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0487 ,  C12Q1/686

Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.

公开(公告)号：US20140272927A1

公开(公告)日：2014-09-18

申请号：US13838411

申请日：2013-03-15

Applicant: CANON U.S. LIFE SCIENCES, INC.

Inventor： Johnathan S. Coursey ,  Kenton C. Hasson ,  Brian Bean ,  Scott Corey

IPC: C12Q3/00 ,  C12Q1/68

CPC classification number: C12Q3/00 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/148 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1827 ,  B01L2400/0487 ,  C12Q1/686

Abstract: Methods, devices, and systems for performing polymerase chain reaction (PCR) amplification and melt data acquisition according to a single slug approach in which a single slug in a microfluidic channel fills an entire thermal zone of the microfluidic channel, and the thermal zone used for both PCR temperature cycling and melt data acquisition. A detector may be configured to detect fluorescence from the thermal zone during the PCR temperature cycling for real-time PCR and/or during temperature ramping in the melt data acquisition. Slug position control may be achieved by detecting leading or trailing edges in a slug build target zone into which a slug passes after passing through the thermal zone. The single slug approach may break coupling between one or more events of the PCR amplification and melt data acquisition and enable events to be independently optimized.

Abstract translation: 用于根据单细胞块方法进行聚合酶链式反应（PCR）扩增和熔融数据采集的方法，装置和系统，其中微流体通道中的单个颗粒填充微流体通道的整个热区，以及用于 PCR温度循环和熔融数据采集。 检测器可以被配置为在用于实时PCR的PCR温度循环期间和/或在熔融数据采集期间的温度斜坡期间检测来自热区域的荧光。 可以通过检测在穿过热区之后塞子通过的塞子构建目标区域中的前缘或后缘来实现塞子位置控制。 单个插段方法可能会破坏PCR扩增的一个或多个事件与熔融数据采集之间的耦合，并使事件能够独立优化。