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1.发明申请公开(公告)号：US20220213465A1
公开(公告)日：2022-07-07
申请号：US17526743
申请日：2021-11-15
摘要： The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.
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2.发明申请公开(公告)号：US20220290208A1
公开(公告)日：2022-09-15
申请号：US17826637
申请日：2022-05-27
发明人： Ian M. White , Stephen Restaino , Marina Pranda
IPC分类号： C12Q1/24
摘要： The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow pathogen cell lysis. In one embodiment, the microfluidic device comprises a microfluidic channel, a microporous structure within the channel, and an enzyme immobilized on the surface of the microporous structure configured to lyse pathogen cells in fluid flowing through the microfluidic channel.
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3.发明授权公开(公告)号：US11174478B2
公开(公告)日：2021-11-16
申请号：US15160935
申请日：2016-05-20
摘要： The present invention relates to a method for concentrating a biological sample containing nucleic acids by using magnetic chitosan microparticles and subsequently performing a PCR reaction on the nucleic acids captured on the microparticles. The chitosan microparticles added to the biological sample at a PCR compatible pH are mechanically agitated to provide for cell lysis and simultaneous DNA capture, and then serve as a solid support for the nucleic acid template during the PCR reaction. As the chitosan microparticles are utilized for lysis and the nucleic acids do not need to be removed from the microparticles before PCR, the ease of the sample preparation procedure is dramatically improved.
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4.发明授权公开(公告)号：US11345944B2
公开(公告)日：2022-05-31
申请号：US14586499
申请日：2014-12-30
发明人： Ian M. White , Stephen Restaino , Marina Pranda
摘要： The present invention relates to methods and systems for cell lysis in a microfluidic device. More specifically, embodiments of the present invention relate to methods and systems for rapid continuous flow pathogen cell lysis. In one embodiment, the microfluidic device comprises a microfluidic channel, a microporous structure within the channel, and an enzyme immobilized on the surface of the microporous structure configured to lyse pathogen cells in fluid flowing through the microfluidic channel.
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5.发明授权公开(公告)号：US10183291B2
公开(公告)日：2019-01-22
申请号：US15857754
申请日：2017-12-29
摘要： The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.
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6.发明授权公开(公告)号：US09855555B2
公开(公告)日：2018-01-02
申请号：US15160891
申请日：2016-05-20
CPC分类号： B01L3/502715 , B01L3/502784 , B01L2200/0621 , B01L2200/0642 , B01L2200/0673 , B01L2300/0816 , B01L2300/0838 , B01L2300/0858 , B01L2300/0864 , B01L2300/088 , B01L2300/10 , B01L2300/14 , B01L2300/18 , B01L2400/0487 , B01L2400/0666 , B01L2400/0688
摘要： The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.
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7.发明申请公开(公告)号：US20230063411A1
公开(公告)日：2023-03-02
申请号：US17811491
申请日：2022-07-08
发明人： Micaela Everitt , David Boegner , Ian M. White
摘要： Disclosed are liquid and solid assay compositions and portable sample reader devices for use in a sample-to-answer diagnostic system for the detection of one or more analytes, preferably the detection of circulating histones in whole blood. Further provided are methods of making and using the assay compositions and portable sample reader, including the collection of a raw sample, testing the sample using the assay compositions, and analyzing the sample via the portable sample reader. More particularly, assay compositions comprising a sacrificial partition, target molecule, detectable label, and sacrificial partition are used in combination with a sample reader comprising an optical system and a housing unit as part of a sample-to-answer diagnostic system for quantifying circulating histones in whole blood as a mechanism of predicting the risk of multiple organ failure.
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8.发明授权公开(公告)号：US10675624B2
公开(公告)日：2020-06-09
申请号：US16252020
申请日：2019-01-18
摘要： The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.
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9.发明申请公开(公告)号：US20190224672A1
公开(公告)日：2019-07-25
申请号：US16252020
申请日：2019-01-18
IPC分类号： B01L3/00
CPC分类号： B01L3/502715 , B01L3/502784 , B01L2200/0621 , B01L2200/0642 , B01L2200/0673 , B01L2300/0816 , B01L2300/0838 , B01L2300/0858 , B01L2300/0864 , B01L2300/088 , B01L2300/10 , B01L2300/14 , B01L2300/18 , B01L2400/0487 , B01L2400/0666 , B01L2400/0688
摘要： The invention relates to a method and system for generating droplets of an aqueous solution on a microfluidic chip with an air continuous phase. Specifically, the droplet generator according to the present invention is integrated into a microfluidic chip to generate and introduce droplets of an aqueous solution into the microfluidic chip. The droplets travelling in a network of chip channels may be captured in on-chip traps in a manner defined by hydrodynamic resistances of chip channels. A biological reaction may be performed on a droplet trapped on the microfluidic chip.
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公开(公告)号：US20180045626A1
公开(公告)日：2018-02-15
申请号：US15797704
申请日：2017-10-30
发明人： Jeffrey M. Burke , Ian M. White
摘要： A spiral inertial filtration device is capable of high-throughput (1 mL/min), high-purity particle separation while concentrating recovered target particles by more than an order of magnitude. Large fractions of sample fluid are removed from a microchannel without disruption of concentrated particle streams by taking advantage of particle focusing in inertial spiral microfluidics, which is achieved by balancing inertial lift forces and Dean drag forces. To enable the calculation of channel geometries in the device for specific concentration factors, an equivalent circuit model was developed and experimentally validated. Large particle concentration factors were achieved by maintaining either average fluid velocity or Dean number throughout the entire length of the channel during the incremental removal of sample fluid. Also provided is the ability to simultaneously separate more than one particle from the same sample.
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