公开(公告)号：US06906185B1

公开(公告)日：2005-06-14

申请号：US09045732

申请日：1998-03-20

申请人： Carl W. Fuller ,  Mark McDougall ,  Shiv Kumar

发明人： Carl W. Fuller ,  Mark McDougall ,  Shiv Kumar

IPC分类号： C07H19/173 ,  C07H21/04 ,  C12P19/32

CPC分类号： C07H19/173 ,  C07H21/04

摘要： Derivatives of 7-deaza-2′-deoxyguanosine are described. Also described is the use of such compounds in synthesis of nucleic acid polymers and in methods for determining a nucleotide base sequence.

摘要翻译： 描述了7-脱氮-2'-脱氧鸟苷的衍生物。 还描述了这样的化合物在核酸聚合物的合成中的用途以及用于测定核苷酸碱基序列的方法。

公开(公告)号：US06600028B1

公开(公告)日：2003-07-29

申请号：US09402048

申请日：2000-02-01

申请人： Daniel Brown ,  David Loakes ,  David Williams ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall ,  Alan Hamilton ,  Clifford Smith ,  Adrian Christopher Simmonds ,  William Jonathan Cummins ,  Patrick Finn

发明人： Daniel Brown ,  David Loakes ,  David Williams ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall ,  Alan Hamilton ,  Clifford Smith ,  Adrian Christopher Simmonds ,  William Jonathan Cummins ,  Patrick Finn

IPC分类号： C07H2100

CPC分类号： C07D498/16 ,  C07H19/04 ,  C07H21/00

摘要： Nucleoside analogues have structure (2) wherein Q is H or a sugar moiety or sugar analogue or a modified sugar or a nucleic backbone or backbone analogue, W is an alkylene or alkenylene chain of 0-5 carbon atoms, any of which may carry a substituent R8, X is O or N or NR12 or CR10, X′ is O or S or N, provided that when X′ is O or S, then X is C, Y is CH or N, R6 is NH2 or SMe or SO2Me or NHNH2, each of R7 and R8 is independently H or F or alkyl or alkenyl or aryl or acyl or a reporter moiety, R12 is independently H or alkyl or alkenyl or aryl or acyl or a reporter moiety, and R10 is H or ═O or F or alkyl or aryl or a reporter moiety.

摘要翻译： 核苷类似物具有结构（2），其中Q是H或糖部分或糖类似物或修饰的糖或核心或骨架类似物，W是0-5个碳原子的亚烷基或亚烯基链，其中任何一个可携带 取代基R8，X为O或N或NR12或CR10，X'为O或S或N，条件是当X'为O或S时，则X为C，Y为CH或N，R6为NH2或SMe或SO2Me 或NHNH 2，R 7和R 8各自独立地为H或F，或烷基或链烯基或芳基或酰基或报道部分，R 12独立地为H或烷基或链烯基或芳基或酰基或报道部分，R 10为H或= O 或F或烷基或芳基或报道部分。

公开(公告)号：US06444682B1

公开(公告)日：2002-09-03

申请号：US09463501

申请日：2000-04-18

申请人： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

发明人： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

IPC分类号： A61K3170

CPC分类号： G01N33/5308 ,  C07H21/00

摘要： Nucleotide or base analogues having structure (3) or (4) wherein X═O or NH or S and each R6 is independently H or alkyl or alkenyl or alkoxy or aryl or a reporter moiety.

摘要翻译： 具有结构（3）或（4）的核苷酸或碱基类似物，其中X = O或NH或S，每个R6独立地为H或烷基或链烯基，烷氧基或芳基或报道部分。

公开(公告)号：US06605611B2

公开(公告)日：2003-08-12

申请号：US10233993

申请日：2002-09-03

申请人： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

发明人： Adrian Christopher Simmonds ,  Alan Hamilton ,  Clifford Smith ,  David Loakes ,  Daniel Brown ,  Fergal Hill ,  Shiv Kumar ,  Satyam Nampalli ,  Mark McDougall

IPC分类号： A61K31495

CPC分类号： G01N33/5308 ,  C07H21/00

摘要： Nucleoside analogues or base analogues having structure (I), where X=O or NH or S, Y=N or CHR6 or CR6, W=N or NR6 or CHR6 or CR6 or S, n=1 or 2; each R6 is independently H or O or alkyl or alkenyl or alkoxy or aryl or a reporter moiety; where necessary (i.e. when Y and/or W is N or CR6 where R6 is not O) a double bond is present between Y and W or W and W, and Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue.

摘要翻译： 具有结构（I）的核苷类似物或碱基类似物，其中X = O或NH或S，Y = N或CHR 6或CR 6，W = N或NR 6或CHR 6或CR 6或S，n = 1或2; 每个R 6独立地为H或O或烷基或链烯基或烷氧基或芳基或报道部分; 必要时（即当Y和/或W为N或R6，R6不为O时），Y与W或W和W之间存在双键，Q为H或糖或糖类似物或核酸骨架 或骨架类似物。

公开(公告)号：US07393640B2

公开(公告)日：2008-07-01

申请号：US10772996

申请日：2004-02-05

申请人： Shiv Kumar ,  Mark McDougall ,  Anup Sood ,  John Nelson ,  Carl Fuller ,  John Macklin ,  Paul Mitsis

发明人： Shiv Kumar ,  Mark McDougall ,  Anup Sood ,  John Nelson ,  Carl Fuller ,  John Macklin ,  Paul Mitsis

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12P19/34

摘要： The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.

摘要翻译： 本发明描述了在锰盐存在下使用末端磷酸酯标记的核苷酸以增强其对各种酶的底物性质的方法。 特别描述的是在锰盐存在的情况下，基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物，检测样品中的核酸的方法。 还提供了末端磷酸酯标记的核苷酸的锰络合物以及具有增强的底物性质的新接头的末端磷酸酯标记的核苷酸。

公开(公告)号：US06664058B2

公开(公告)日：2003-12-16

申请号：US09898210

申请日：2001-07-03

申请人： Shiv Kumar ,  Satyam Nampalli ,  Constantin Neagu ,  Mark McDougall ,  David Loakes ,  Dan Brown

发明人： Shiv Kumar ,  Satyam Nampalli ,  Constantin Neagu ,  Mark McDougall ,  David Loakes ,  Dan Brown

IPC分类号： C12Q168

CPC分类号： C07D487/04 ,  C07F9/6561 ,  C07H19/23 ,  C12Q2525/117 ,  G01N33/58

摘要： The present invention describes novel compounds of the formula Wherein Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue, Y=O, S, NR10, where R10 is H, alkyl alkenyl, alkynyl, X is H, alkyl, alkenyl, alkynyl, aryl, heteroaryl or a combination thereof or, preferably, a reporter group. The novel compounds are suitable for incorporation in oligonucleotides and polynucleotides.

摘要翻译： 本发明描述了新颖的式化合物，其中Q是H或糖或糖类似物或核酸主链或主链类似物，Y = O，S，NR 10，其中R 10是H，烷基烯基， 炔基，X是H，烷基，烯基，炔基，芳基，杂芳基或其组合，或优选报道基团。 新型化合物适用于掺入寡核苷酸和多核苷酸。

公开(公告)号：US20120220013A1

公开(公告)日：2012-08-30

申请号：US13435970

申请日：2012-03-30

申请人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi V. Los ,  Mark McDougall ,  Keith V. Wood ,  Monika G. Wood ,  Chad Zimprich

IPC分类号： C12N9/14 ,  C12N15/62 ,  C12N15/55

CPC分类号： G01N33/581 ,  C07K7/06 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/43 ,  C07K2319/61 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06 ,  C09B23/105 ,  C12N9/14 ,  C12Q1/34 ,  C12Y308/01005 ,  G01N33/573 ,  G01N2333/914 ,  Y10S530/825

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US20060024808A1

公开(公告)日：2006-02-02

申请号：US11194110

申请日：2005-07-29

申请人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi Los ,  Mark McDougall ,  Keith Wood ,  Monika Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Dieter Klaubert ,  Georgyi Los ,  Mark McDougall ,  Keith Wood ,  Monika Wood ,  Chad Zimprich

IPC分类号： C12Q1/48 ,  C07H21/04 ,  C12P21/06 ,  C12N9/14 ,  C12N15/74

CPC分类号： G01N33/581 ,  C07K7/06 ,  C07K16/00 ,  C07K2317/622 ,  C07K2319/43 ,  C07K2319/61 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06 ,  C09B23/105 ,  C12N9/14 ,  C12Q1/34 ,  C12Y308/01005 ,  G01N33/573 ,  G01N2333/914 ,  Y10S530/825

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

公开(公告)号：US20050272114A1

公开(公告)日：2005-12-08

申请号：US11006031

申请日：2004-12-06

申请人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi Los ,  Mark McDougall ,  Keith Wood ,  Monika Wood ,  Chad Zimprich

发明人： Aldis Darzins ,  Lance Encell ,  Tonny Johnson ,  Dieter Klaubert ,  Georgyi Los ,  Mark McDougall ,  Keith Wood ,  Monika Wood ,  Chad Zimprich

IPC分类号： C07K14/47 ,  C12N9/14 ,  C12Q1/26

CPC分类号： C12N9/14 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C07K2299/00 ,  C12N9/0069 ,  C12Q1/26 ,  C12Q1/34 ,  C12Q1/66 ,  C12Y113/12005 ,  C12Y308/01005

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：US08309059B2

公开(公告)日：2012-11-13

申请号：US12872467

申请日：2010-08-31

申请人： Cesear Corona ,  Dieter Klaubert ,  Mark McDougall

发明人： Cesear Corona ,  Dieter Klaubert ,  Mark McDougall

IPC分类号： A61B5/00 ,  A61B8/00 ,  A61B10/00 ,  C07D209/02

CPC分类号： G01N33/582 ,  A61K49/0032 ,  A61K49/0056 ,  C09B23/0008 ,  C09B23/0066 ,  C09B23/06 ,  C09B23/083

摘要： The invention provides compounds and compositions of Formulas I-VII, and methods of using the compounds. The compounds can be used to prepare dye conjugates that are uniformly and substantially more fluorescent on proteins, nucleic acids or other biopolymers, than conjugates labeled with structurally similar known carbocyanine dyes. In addition to having more intense fluorescence emission than structurally similar dyes at virtually identical wavelengths, and decreased artifacts in their absorption spectra upon conjugation to biopolymers, the compounds can have greater photostability and/or higher absorbance (extinction coefficients) at the wavelength(s) of peak absorbance than such structurally similar dyes.

摘要翻译： 本发明提供式I-VII的化合物和组合物，以及使用该化合物的方法。 该化合物可用于制备在蛋白质，核酸或其他生物聚合物上均匀且基本上更多荧光的染料缀合物，而不是与结构上相似的已知碳菁染料标记的缀合物。 除了与实际相同的波长下的结构相似的染料具有更强烈的荧光发射以及在与生物聚合物共轭时其吸收光谱中的伪影减少，化合物在波长处具有更大的光稳定性和/或更高的吸光度（消光系数） 的吸光度比这种结构相似的染料高。