专利检索 ap:("Shiv Kumar" OR "Satyam Nampalli" OR "Constantin Neagu" OR "Mark McDougall" OR "David Loakes" OR "Dan Brown") AND inv:"Shiv Kumar" 第 1 页

1.

发明授权
Base analogues 失效
标题翻译：基础类似物

公开(公告)号：US06664058B2

公开(公告)日：2003-12-16

申请号：US09898210

申请日：2001-07-03

申请人： Shiv Kumar , Satyam Nampalli , Constantin Neagu , Mark McDougall , David Loakes , Dan Brown

发明人： Shiv Kumar , Satyam Nampalli , Constantin Neagu , Mark McDougall , David Loakes , Dan Brown

IPC分类号： C12Q168

CPC分类号： C07D487/04 , C07F9/6561 , C07H19/23 , C12Q2525/117 , G01N33/58

摘要： The present invention describes novel compounds of the formula Wherein Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue, Y=O, S, NR10, where R10 is H, alkyl alkenyl, alkynyl, X is H, alkyl, alkenyl, alkynyl, aryl, heteroaryl or a combination thereof or, preferably, a reporter group. The novel compounds are suitable for incorporation in oligonucleotides and polynucleotides.

摘要翻译： 本发明描述了新颖的式化合物，其中Q是H或糖或糖类似物或核酸主链或主链类似物，Y = O，S，NR 10，其中R 10是H，烷基烯基，炔基，X是H，烷基，烯基，炔基，芳基，杂芳基或其组合，或优选报道基团。新型化合物适用于掺入寡核苷酸和多核苷酸。

2.

发明授权
Tricyclic base analogues 失效
标题翻译：三环基类似物

公开(公告)号：US06600028B1

公开(公告)日：2003-07-29

申请号：US09402048

申请日：2000-02-01

申请人： Daniel Brown , David Loakes , David Williams , Fergal Hill , Shiv Kumar , Satyam Nampalli , Mark McDougall , Alan Hamilton , Clifford Smith , Adrian Christopher Simmonds , William Jonathan Cummins , Patrick Finn

发明人： Daniel Brown , David Loakes , David Williams , Fergal Hill , Shiv Kumar , Satyam Nampalli , Mark McDougall , Alan Hamilton , Clifford Smith , Adrian Christopher Simmonds , William Jonathan Cummins , Patrick Finn

IPC分类号： C07H2100

CPC分类号： C07D498/16 , C07H19/04 , C07H21/00

摘要： Nucleoside analogues have structure (2) wherein Q is H or a sugar moiety or sugar analogue or a modified sugar or a nucleic backbone or backbone analogue, W is an alkylene or alkenylene chain of 0-5 carbon atoms, any of which may carry a substituent R8, X is O or N or NR12 or CR10, X′ is O or S or N, provided that when X′ is O or S, then X is C, Y is CH or N, R6 is NH2 or SMe or SO2Me or NHNH2, each of R7 and R8 is independently H or F or alkyl or alkenyl or aryl or acyl or a reporter moiety, R12 is independently H or alkyl or alkenyl or aryl or acyl or a reporter moiety, and R10 is H or ═O or F or alkyl or aryl or a reporter moiety.

摘要翻译： 核苷类似物具有结构（2），其中Q是H或糖部分或糖类似物或修饰的糖或核心或骨架类似物，W是0-5个碳原子的亚烷基或亚烯基链，其中任何一个可携带取代基R8，X为O或N或NR12或CR10，X'为O或S或N，条件是当X'为O或S时，则X为C，Y为CH或N，R6为NH2或SMe或SO2Me 或NHNH 2，R 7和R 8各自独立地为H或F，或烷基或链烯基或芳基或酰基或报道部分，R 12独立地为H或烷基或链烯基或芳基或酰基或报道部分，R 10为H或= O 或F或烷基或芳基或报道部分。

3.

发明授权
Base analogues 失效
标题翻译：基础类似物

公开(公告)号：US06444682B1

公开(公告)日：2002-09-03

申请号：US09463501

申请日：2000-04-18

申请人： Adrian Christopher Simmonds , Alan Hamilton , Clifford Smith , David Loakes , Daniel Brown , Fergal Hill , Shiv Kumar , Satyam Nampalli , Mark McDougall

发明人： Adrian Christopher Simmonds , Alan Hamilton , Clifford Smith , David Loakes , Daniel Brown , Fergal Hill , Shiv Kumar , Satyam Nampalli , Mark McDougall

IPC分类号： A61K3170

CPC分类号： G01N33/5308 , C07H21/00

摘要： Nucleotide or base analogues having structure (3) or (4) wherein X═O or NH or S and each R6 is independently H or alkyl or alkenyl or alkoxy or aryl or a reporter moiety.

摘要翻译： 具有结构（3）或（4）的核苷酸或碱基类似物，其中X = O或NH或S，每个R6独立地为H或烷基或链烯基，烷氧基或芳基或报道部分。

4.

发明授权
Base analogues 失效
标题翻译：基础类似物

公开(公告)号：US06605611B2

公开(公告)日：2003-08-12

申请号：US10233993

申请日：2002-09-03

申请人： Adrian Christopher Simmonds , Alan Hamilton , Clifford Smith , David Loakes , Daniel Brown , Fergal Hill , Shiv Kumar , Satyam Nampalli , Mark McDougall

发明人： Adrian Christopher Simmonds , Alan Hamilton , Clifford Smith , David Loakes , Daniel Brown , Fergal Hill , Shiv Kumar , Satyam Nampalli , Mark McDougall

IPC分类号： A61K31495

CPC分类号： G01N33/5308 , C07H21/00

摘要： Nucleoside analogues or base analogues having structure (I), where X=O or NH or S, Y=N or CHR6 or CR6, W=N or NR6 or CHR6 or CR6 or S, n=1 or 2; each R6 is independently H or O or alkyl or alkenyl or alkoxy or aryl or a reporter moiety; where necessary (i.e. when Y and/or W is N or CR6 where R6 is not O) a double bond is present between Y and W or W and W, and Q is H or a sugar or a sugar analogue or a nucleic acid backbone or backbone analogue.

摘要翻译： 具有结构（I）的核苷类似物或碱基类似物，其中X = O或NH或S，Y = N或CHR 6或CR 6，W = N或NR 6或CHR 6或CR 6或S，n = 1或2; 每个R 6独立地为H或O或烷基或链烯基或烷氧基或芳基或报道部分; 必要时（即当Y和/或W为N或R6，R6不为O时），Y与W或W和W之间存在双键，Q为H或糖或糖类似物或核酸骨架或骨架类似物。

5.

发明授权
Heterocyclic FRETdye cassettes for labeling biological molecules and their use in DNA sequencing 有权
标题翻译：用于标记生物分子的杂环FRETdye盒及其在DNA测序中的应用

公开(公告)号：US06855503B2

公开(公告)日：2005-02-15

申请号：US10326039

申请日：2002-12-20

申请人： Satyam Nampalli , Weihong Zhang , Sudhakar Rao , Shiv Kumar

发明人： Satyam Nampalli , Weihong Zhang , Sudhakar Rao , Shiv Kumar

IPC分类号： C09B11/08 , C09B11/24 , C09B23/08 , C12Q1/68 , C07D1/02

CPC分类号： C09B23/083 , C09B11/08 , C09B11/24 , C12Q1/6818 , C12Q1/6869

摘要： Exploitation of suitably functionalized heterocyclic molecules, in the design and synthesis of Fluorescence Resonance Energy Transfer (FRET) cassettes and their corresponding dideoxynucleotide terminators culminated into efficient reagents for DNA sequencing. Additionally, these FRET cassettes/terminators, of the present invention, derived from different classes of heterocyclic systems have high potential to be used for general labelling of biological molecules to generate highly sensitized signals. Their preparation, energy transfer efficiency, and use as labels, specifically, in DNA sequencing reactions is disclosed.

摘要翻译： 在荧光共振能量转移（FRET）盒及其相应的双脱氧核苷酸终止子的设计和合成中，适当官能化的杂环分子的开发最终成为DNA测序的有效试剂。此外，衍生自不同种类的杂环体系的本发明的这些FRET盒/终止子具有很高的潜力用于生物分子的一般标记以产生高致敏信号。公开了它们的制备，能量转移效率和用作标记，特别是在DNA测序反应中。

6.

发明授权
Energy transfer dyes 失效
标题翻译：能量转移染料

公开(公告)号：US06967250B1

公开(公告)日：2005-11-22

申请号：US09386576

申请日：1999-08-30

申请人： Shiv Kumar , Satyam Nampalli , Mahesh Khot

发明人： Shiv Kumar , Satyam Nampalli , Mahesh Khot

IPC分类号： C07D265/36 , G01N33/542

CPC分类号： G01N33/542

摘要： Energy transfer dyes, their preparation, and their use as labels in biological systems is disclosed. The dyes are preferably in the form of cassettes which enable their attachment to a variety of biological materials. The dyes and the reagents that can be made from them offer a wide variety of fluorescent labels with large Stokes' shifts enabling their use in a variety of fluorescence applications over a wide range of the visible spectrum.

摘要翻译： 公开了能量转移染料，它们的制备及其作为生物系统中的标记物的用途。染料优选为使其能够附着于各种生物材料的盒的形式。可以由其制成的染料和试剂提供了广泛的荧光标记，具有大的斯托克斯变化，使其能够在广泛的可见光谱范围内用于各种荧光应用。

7.

发明授权
Derivatives of 7-deaza -2′-deoxyguanosine-5'-triphosphate, preparation and use thereof 失效
标题翻译： 7-脱氮-2'-脱氧鸟苷-5'-三磷酸的衍生物，的制备和用途

公开(公告)号：US06906185B1

公开(公告)日：2005-06-14

申请号：US09045732

申请日：1998-03-20

申请人： Carl W. Fuller , Mark McDougall , Shiv Kumar

发明人： Carl W. Fuller , Mark McDougall , Shiv Kumar

IPC分类号： C07H19/173 , C07H21/04 , C12P19/32

CPC分类号： C07H19/173 , C07H21/04

摘要： Derivatives of 7-deaza-2′-deoxyguanosine are described. Also described is the use of such compounds in synthesis of nucleic acid polymers and in methods for determining a nucleotide base sequence.

摘要翻译： 描述了7-脱氮-2'-脱氧鸟苷的衍生物。还描述了这样的化合物在核酸聚合物的合成中的用途以及用于测定核苷酸碱基序列的方法。

8.

发明授权
Terminal-phosphate-labeled nucleotides with new linkers 有权
标题翻译：末端磷酸酯标记的核苷酸与新的接头

公开(公告)号：US07393640B2

公开(公告)日：2008-07-01

申请号：US10772996

申请日：2004-02-05

申请人： Shiv Kumar , Mark McDougall , Anup Sood , John Nelson , Carl Fuller , John Macklin , Paul Mitsis

发明人： Shiv Kumar , Mark McDougall , Anup Sood , John Nelson , Carl Fuller , John Macklin , Paul Mitsis

IPC分类号： C12Q1/68 , C12P19/34

CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12P19/34

摘要： The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.

摘要翻译： 本发明描述了在锰盐存在下使用末端磷酸酯标记的核苷酸以增强其对各种酶的底物性质的方法。特别描述的是在锰盐存在的情况下，基于使用末端磷酸酯标记的核苷酸作为核酸聚合酶的底物，检测样品中的核酸的方法。还提供了末端磷酸酯标记的核苷酸的锰络合物以及具有增强的底物性质的新接头的末端磷酸酯标记的核苷酸。