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33 条记录 (耗时 0.025 秒)

    Microfluidic chip and method of fabricating the same
    6.
    发明授权
    Microfluidic chip and method of fabricating the same 有权
    微流控芯片及其制造方法

    公开(公告)号：US07858042B2

    公开(公告)日：2010-12-28

    申请号：US11934811

    申请日：2007-11-05

    申请人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chin-sung Park ,  Sung-young Jeong

    发明人： Kyu-youn Hwang ,  Joon-ho Kim ,  Chin-sung Park ,  Sung-young Jeong

    IPC分类号： B01N3/00

    CPC分类号： B01L3/502707 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/16 ,  Y10T428/14

    摘要： Provided are a microfluidic chip and a method of fabricating the same. The microfluidic chip includes: a lower substrate; an upper substrate formed of a silicone resin, wherein the lower substrate and the upper substrate, bonded together, provide a channel through which a fluid can flow and a chamber to receive the fluid; and an organic thin film formed on the upper surface of the lower substrate except for portions on which the lower substrate and the upper substrate are attached to each other.

    摘要翻译： 提供了一种微流控芯片及其制造方法。 微流体芯片包括：下基板; 由硅树脂形成的上基板，其中所述下基板和所述上基板接合在一起，提供流体可以流过的通道和容纳所述流体的室; 以及形成在下基板的上表面上的有机薄膜，除了下基板和上基板彼此附接的部分之外。

    Device and method for pre-treating and injecting liquid specimen
    7.
    发明授权
    Device and method for pre-treating and injecting liquid specimen 有权
    预处理和注射液体样品的装置和方法

    公开(公告)号：US07837943B2

    公开(公告)日：2010-11-23

    申请号：US11484068

    申请日：2006-07-11

    申请人： Sung-young Jeong ,  Hun-joo Lee

    发明人： Sung-young Jeong ,  Hun-joo Lee

    IPC分类号： A61M5/19 ,  A61M5/315

    CPC分类号： G01N1/14 ,  G01N1/405

    摘要： A device for pre-treating and injecting a liquid specimen includes a first syringe including an open top first tube including a first hole in the bottom and protrusions, a second syringe including an open top second tube including a second hole in the bottom and an inner diameter greater than an outer diameter of the first tube, a second piston movable along an inner wall of the second tube and including a third hole and a communication channel providing fluid communication between the first hole and the third hole, and a joint portion attachable to and detachable from a top of the second tube and including an opening corresponding to a transverse cross-section of the first tube and grooves corresponding to the shape of the protrusions such that the first tube passes through the joint portion when the protrusions are matched with the grooves.

    摘要翻译： 一种用于预处理和注射液体试样的装置包括：第一注射器，其包括开口顶部第一管，其包括底部中的第一孔和突起;第二注射器，其包括开口顶部第二管，其包括底部中的第二孔， 直径大于第一管的外径，第二活塞可沿着第二管的内壁移动并且包括第三孔和提供第一孔和第三孔之间的流体连通的连通通道，以及连接部分，其可附接到 并且可从第二管的顶部拆卸并且包括对应于第一管的横截面的开口和对应于突起的形状的槽，使得当突起与第二管匹配时，第一管穿过接合部 凹槽

    Method of amplifying nucleic acid from a cell using a nonplanar solid substrate
    9.
    发明授权
    Method of amplifying nucleic acid from a cell using a nonplanar solid substrate 有权
    使用非平面固体底物从细胞中扩增核酸的方法

    公开(公告)号：US07919278B2

    公开(公告)日：2011-04-05

    申请号：US11841107

    申请日：2007-08-20

    申请人： Sung-young Jeong ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Jung-im Han ,  Hun-joo Lee

    发明人： Sung-young Jeong ,  Kyu-youn Hwang ,  Joon-ho Kim ,  Jung-im Han ,  Hun-joo Lee

    IPC分类号： C12P19/34 ,  C12Q1/68

    CPC分类号： C12N15/1006 ,  B01L7/52 ,  B01L2300/0636 ,  C12N2533/30 ,  C12Q1/6806

    摘要： Provided is a method of amplifying nucleic acid from a comprising: contacting a cell-containing sample with a nonplanar solid substrate in a liquid medium having a pH range of 3.0-6.0 to attach the cell to the solid substrate; washing the nonplanar solid substrate to remove materials that are not attached thereto; and performing PCR using the nucleic acid from the cell attached to the nonplanar solid substrate as a template sample to amplify nucleic acid from the cell, wherein the contacting, washing and PCR processes are performed in a single vessel.

    摘要翻译： 本发明提供一种扩增来自a的核酸的方法，其特征在于：在pH范围为3.0-6.0的液体介质中使含细胞的样品与非平面固体基质接触，以将该细胞附着在固体基质上; 洗涤非平面固体基质以除去未附着于其上的材料; 以及使用来自附着在所述非平面固体底物上的细胞的核酸作为模板样品进行PCR以扩增来自所述细胞的核酸，其中所述接触，洗涤和PCR过程在单个容器中进行。