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    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex
    3.
    发明申请
    Method of and apparatus for the purification of nucleic acids using immobilized metal-ligand complex 审中-公开
    使用固定化金属 - 配体复合物纯化核酸的方法和设备

    公开(公告)号：US20060275815A1

    公开(公告)日：2006-12-07

    申请号：US11446795

    申请日：2006-06-05

    申请人： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    发明人： Chang-eun Yoo ,  Sang-hyun Peak ,  Sook-young Kim ,  Jong-myeon Park

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C07H21/04 ,  C12N15/1006

    摘要： Provided is a method of purifying nucleic acids using a metal-ligand complex, the method comprising: immobilizing the metal-ligand complex on a solid support; bringing a sample containing the nucleic acid into contact with the immobilized complex on the solid support to bind the nucleic acid to the complex; and adding a solution containing a chelate capable of removing the metal to elute the nucleic acid bound to the complex. The nucleic acids can be efficiently purified using a metal-ligand complex interacting with a base of the nucleic acid instead of interacting with a phosphoric acid of the nucleic acid to selectively bind the nucleic acid, and using a chelator capable of removing the metal to elute the nucleic acid.

    摘要翻译： 提供了使用金属 - 配体络合物纯化核酸的方法，所述方法包括：将所述金属 - 配体复合物固定在固体支持物上; 使含有核酸的样品与固体支持物上的固定化复合物接触以将核酸结合到复合物上; 并加入含有能除去金属的螯合物的溶液以洗脱与络合物结合的核酸。 可以使用与核酸的碱基相互作用的金属 - 配体复合物来有效纯化核酸，而不是与核酸的磷酸相互作用以选择性地结合核酸，并且使用能够去除金属洗脱的螯合剂 核酸。

    Microfluidic valve filler and valve unit including the same
    8.
    发明授权
    Microfluidic valve filler and valve unit including the same 有权
    微流量阀的填料和阀门单元包括它们

    公开(公告)号：US08281815B2

    公开(公告)日：2012-10-09

    申请号：US12027290

    申请日：2008-02-07

    申请人： Jong-myeon Park ,  Beom-seok Lee

    发明人： Jong-myeon Park ,  Beom-seok Lee

    IPC分类号： F15C1/04

    CPC分类号： F16K99/0019 ,  B01L3/502738 ,  B01L2300/1861 ,  B01L2400/0677 ,  F16K99/0001 ,  F16K99/0032 ,  F16K99/004 ,  F16K2099/0069 ,  F16K2099/0084 ,  Y10T137/2196

    摘要： Provided are a valve filler and a valve unit including the valve filler. The valve filler includes: a phase transition material; and a heating fluid comprising a carrier oil and a plurality of micro heating particles suspended in the carrier oil, the heating fluid being mixed with the phase transition material, wherein, when external energy is supplied, the micro heating particles receive the external energy and generate heat to melt the phase transition material into a fluid state, and when no external energy is supplied, the phase transition material hardens into a solid state.

    摘要翻译： 提供了一种阀填料和包括阀填料的阀单元。 阀填料包括：相变材料; 以及加热流体，其包含载体油和悬浮在载体油中的多个微加热颗粒，所述加热流体与所述相变材料混合，其中，当供应外部能量时，所述微加热颗粒接收外部能量并产生 将相变材料熔化成流体状态的热量，当没有外部能量供给时，相变材料变硬。

    Centrifugal force-based microfluidic device for protein detection and microfluidic system including the same
    10.
    发明授权
    Centrifugal force-based microfluidic device for protein detection and microfluidic system including the same 有权
    用于蛋白质检测和微流体系统的基于离心力的微流体装置包括相同的

    公开(公告)号：US07951333B2

    公开(公告)日：2011-05-31

    申请号：US12851819

    申请日：2010-08-06

    申请人： Beom Seok Lee ,  Yoon Kyoung Cho ,  Jeong Gun Lee ,  Jong-myeon Park

    发明人： Beom Seok Lee ,  Yoon Kyoung Cho ,  Jeong Gun Lee ,  Jong-myeon Park

    IPC分类号： G01N9/30

    CPC分类号： B01L3/502738 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2300/1861 ,  B01L2400/0409 ,  B01L2400/0442 ,  B01L2400/0633 ,  B01L2400/0677 ,  B01L2400/0688 ,  G01N33/54326 ,  G01N33/54366 ,  Y10T436/25375

    摘要： A centrifugal force-based microfluidic device for the detection of a target biomolecule and a microfluidic system including the same are provided. The device includes a body of revolution; a microfluidic structure disposed in the body of revolution including chambers, channels connecting the chambers, and valves disposed in the channels to control fluid flow, the microfluidic structures transmitting fluid using centrifugal force due to rotation of the body of revolution; and beads disposed in the microfluidic structures, the beads having capture probes on the surfaces thereof which are selectively bonded with target protein; and a detection probe disposed in the microfluidic structures and selectively bonded to the target protein, and which includes a material required to express an optical signal, wherein the microfluidic structure mixes the beads, biological samples, and the detection probe to react and washes and separates the beads after the reaction.

    摘要翻译： 提供了用于检测靶生物分子的离心力微流体装置和包括其的微流体系统。 该装置包括一个革命体; 设置在旋转体中的微流体结构，包括腔室，连接腔室的通道，以及设置在通道中的阀，以控制流体流动，微流体结构由于旋转体旋转而使用离心力传送流体; 和珠子，其设置在微流体结构中，珠粒在其表面上具有与靶蛋白质选择性结合的捕获探针; 以及设置在微流体结构中并选择性地结合到靶蛋白上并且包括表达光信号所需的材料的检测探针，其中微流体结构混合珠，生物样品和检测探针以反应和洗涤和分离 反应后的珠子。