公开(公告)号：US4473647A

公开(公告)日：1984-09-25

申请号：US349691

申请日：1982-02-17

申请人： Charles R. Carpenter ,  Robert O. Cone, Jr.

发明人： Charles R. Carpenter ,  Robert O. Cone, Jr.

IPC分类号： G01N33/50 ,  A61K38/21 ,  C12N5/00 ,  C12N7/00 ,  C12N15/00 ,  C12N15/02 ,  A61K35/16 ,  C07G7/00 ,  C12N1/38

CPC分类号： C12N5/0018 ,  C12N2500/84 ,  Y10S530/83

摘要： A natural bovine serum-derived serum which has low lipid levels and may additionally have similar globulin and albumin profile as fetal calf serum, as well as controlled levels of hemoglobin, enveloped viruses, steroid hormones, mycoplasma, cholesterol, triglycerides and pesticides, is useful for the promotion of growth of animal and plant cells in tissue culture.

摘要翻译： 具有低脂质水平的天然牛血清来源的血清，并且可以另外具有与胎牛血清相似的球蛋白和白蛋白谱，以及血红蛋白，包膜病毒，类固醇激素，支原体，胆固醇，甘油三酯和农药的受控水平是有用的 用于促进组织培养中动物和植物细胞的生长。

公开(公告)号：US4481298A

公开(公告)日：1984-11-06

申请号：US351153

申请日：1982-02-19

申请人： Robert O. Cone, Jr. ,  Charles R. Carpenter

发明人： Robert O. Cone, Jr. ,  Charles R. Carpenter

IPC分类号： G01N33/531 ,  G01N33/537 ,  G01N33/563 ,  G01N33/78 ,  G01N33/54

CPC分类号： G01N33/537 ,  G01N33/531 ,  G01N33/563 ,  G01N33/78 ,  Y10S435/975 ,  Y10S436/804 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/817 ,  Y10S530/806 ,  Y10S530/807 ,  Y10S530/866

摘要： An immunoassay process for the detection of an antigen in a sample, which comprises: (a) forming a mixture of the sample with (1) a preformed complex of a primary antibody and a secondary binding macromolecule therefor, wherein the primary antibody is present at low concentrations and has substantial specificity for the antigen, the secondary binding macromolecule has substantial affinity for the Fc portion of the primary antibody, and the second binding macromolecule is affinity purified; and with (2) a detectably labeled form of the antigen; (b) incubating the mixture formed in step (a) for a time sufficient to allow the antigen and the detectably labeled antigen to competitively bind to the primary antibody of the preformed complex; (c) detecting the separated complex or the separated suspension medium.

摘要翻译： 一种用于检测样品中的抗原的免疫测定方法，其包括：（a）形成所述样品与（1）预先形成的第一抗体复合物和其第二结合大分子的混合物，其中所述第一抗体存在于 低浓度且对抗原具有相当大的特异性，二级结合大分子对第一抗体的Fc部分具有实质的亲和力，第二结合大分子是亲和纯化的; 和（2）抗原的可检测标记形式; （b）将步骤（a）中形成的混合物温育足以允许抗原和可检测标记的抗原与预先形成的复合物的一级抗体竞争性结合的时间; （c）检测分离的复合物或分离的悬浮介质。

公开(公告)号：US20100124652A1

公开(公告)日：2010-05-20

申请号：US12657309

申请日：2010-01-19

申请人： Usama E. Younes ,  David M. Baily ,  Charles R. Carpenter

发明人： Usama E. Younes ,  David M. Baily ,  Charles R. Carpenter

IPC分类号： B32B3/26 ,  C08G18/32 ,  C07C265/02 ,  A43B13/02

CPC分类号： C08G18/12 ,  C08G18/4841 ,  C08G18/6674 ,  C08G2101/0008 ,  C08G2101/0066 ,  C08G2410/00 ,  C08J9/122 ,  C08J2375/04 ,  Y10T428/249978

摘要： Microcellular polyurethane flexible foams having densities no greater than 0.3 g/cc which are suitable for use as lightweight shoe sole components are produced with carbon dioxide in an amount such that the polyurethane-forming mixture has a free rise density of from about 0.03 to about 0.3 g/cc. At least a portion of that carbon dioxide is dissolved as a gas into one or both of the reaction components. The amount of dissolved carbon dioxide must be such that the froth density of the isocyanate and/or isocyanate-reactive component(s) in which the carbon dioxide is dissolved will be from about 0.1 to about 0.8 g/cc. Additional carbon dioxide may be formed by the reaction of water and isocyanate during the polyurethane-forming reaction but the total amount of CO2 present should be controlled to ensure that the polyurethane-forming mixture has a free rise density of from about 0.03 to 0.3 g/cc. Use of a preferred isocyanate-reactive component in which a specified ratio of diol to triol is satisfied makes it possible to use more water than had been expected. Use of a preferred prepolymer makes it possible to produce microcellular polyurethanes having good physical properties solely with a diol. The product microcellular foams possess a uniform cell structure and enhanced physical properties as compared to all water-blown foams of the same basic formulation and density. The hardness of the foams is more suitable for shoe sole, particularly midsole applications, than that of the water-blown foams, despite the lower urea hard segment content of the CO2 blown foams.

公开(公告)号：US20070269840A1

公开(公告)日：2007-11-22

申请号：US11437203

申请日：2006-05-19

申请人： Charles R. Carpenter ,  Giosi Farace

发明人： Charles R. Carpenter ,  Giosi Farace

IPC分类号： G01N33/53

CPC分类号： G01N33/541

摘要： A method for the determination of analytes in samples using a pre-precipitated antibody complex. The complex includes an anti-analyte antibody and a secondary binding molecule that results in the precipitation of the antibody. Labeled-analyte analog competes for binding sites on the complex with analyte in the sample. The presence or amount of the analyte in the sample can is determined by detecting the amount of the label associated with the complex. The pre-precipitated antibody complex may be preloaded with labeled-analyte analog.

摘要翻译： 使用预沉淀抗体复合物测定样品中分析物的方法。 复合物包括导致抗体沉淀的抗分析物抗体和二级结合分子。 标记分析物类似物与样品中分析物的复合物上的结合位点竞争。 通过检测与复合物相关的标签的量来确定样品中分析物的存在或量。 预沉淀抗体复合物可以用标记的分析物类似物预加载。

公开(公告)号：US08178591B2

公开(公告)日：2012-05-15

申请号：US12657309

申请日：2010-01-19

申请人： Usama E. Younes ,  David M. Baily ,  Charles R. Carpenter

发明人： Usama E. Younes ,  David M. Baily ,  Charles R. Carpenter

IPC分类号： C08J9/12

CPC分类号： C08G18/12 ,  C08G18/4841 ,  C08G18/6674 ,  C08G2101/0008 ,  C08G2101/0066 ,  C08G2410/00 ,  C08J9/122 ,  C08J2375/04 ,  Y10T428/249978

摘要： Microcellular polyurethane flexible foams having densities no greater than 0.3 g/cc which are suitable for use as lightweight shoe sole components are produced with carbon dioxide in an amount such that the polyurethane-forming mixture has a free rise density of from about 0.03 to about 0.3 g/cc. At least a portion of that carbon dioxide is dissolved as a gas into one or both of the reaction components. The amount of dissolved carbon dioxide must be such that the froth density of the isocyanate and/or isocyanate-reactive component(s) in which the carbon dioxide is dissolved will be from about 0.1 to about 0.8 g/cc. Additional carbon dioxide may be formed by the reaction of water and isocyanate during the polyurethane-forming reaction but the total amount of CO2 present should be controlled to ensure that the polyurethane-forming mixture has a free rise density of from about 0.03 to 0.3 g/cc. Use of a preferred isocyanate-reactive component in which a specified ratio of diol to triol is satisfied makes it possible to use more water than had been expected. Use of a preferred prepolymer makes it possible to produce microcellular polyurethanes having good physical properties solely with a diol. The product microcellular foams possess a uniform cell structure and enhanced physical properties as compared to all water-blown foams of the same basic formulation and density. The hardness of the foams is more suitable for shoe sole, particularly midsole applications, than that of the water-blown foams, despite the lower urea hard segment content of the CO2 blown foams.

摘要翻译： 使用适合用作轻量级鞋底部件的密度不大于0.3g / cc的微孔聚氨酯柔性泡沫体，其二氧化碳的量使得聚氨酯形成混合物的自由上升密度为约0.03至约0.3 g / cc。 至少一部分二氧化碳作为气体溶解在一个或两个反应组分中。 溶解的二氧化碳的量必须使得二氧化碳溶解的异氰酸酯和/或异氰酸酯反应性组分的泡沫密度为约0.1至约0.8g / cc。 另外的二氧化碳可以在聚氨酯形成反应期间通过水和异氰酸酯的反应形成，但是应当控制存在的CO 2的总量，以确保聚氨酯形成混合物的自由起始密度为约0.03至0.3g / cc。 使用其中满足特定比例的二醇与三醇的优选异氰酸酯反应性组分可以使用比预期更多的水。 使用优选的预聚物使得可以仅用二醇生产具有良好物理性能的微孔聚氨酯。 与具有相同基本配方和密度的所有水吹泡沫相比，产品微孔泡沫具有均匀的细胞结构和增强的物理性能。 尽管二氧化碳吹泡沫的尿素硬链段含量较低，泡沫塑料的硬度更适用于鞋底，特别是中底层应用。

公开(公告)号：US06413778B1

公开(公告)日：2002-07-02

申请号：US09235124

申请日：1999-01-21

申请人： Charles R. Carpenter ,  Melanie Tornberg ,  Genevieve Clark

发明人： Charles R. Carpenter ,  Melanie Tornberg ,  Genevieve Clark

IPC分类号： G01N3348

CPC分类号： G01N33/493 ,  Y10T436/25375

摘要： The present invention relates to devices, methods and kits for the detection and identification of crystals which may be present in a biological fluid, such as urine from a cat. The device may include a filter and an absorbent material. The fluid may be transferred onto the filter, and fluid passes through the filter and into the absorbent material, thereby isolating crystals on a surface of or within the structure of the filter or membrane. The crystals are then available for staining which will reveal a determining component of the crystals. The device may also include a member for inhibiting backflow from the absorbent material to the filter. The present invention also provides methods for detecting and identifying crystals which may be present in biological fluids. The methods include the steps of isolating the crystals on the surface of or within the structure of a filter or membrane, and contacting the crystals with an indicator which is specific for a determining component of the crystals type. Also provided are kits which include the devices of the present invention, and reagents and other materials necessary to conduct the methods of the present invention.

摘要翻译： 本发明涉及用于检测和鉴定可能存在于生物流体例如来自猫的尿液中的晶体的装置，方法和试剂盒。 该装置可以包括过滤器和吸收材料。 流体可以转移到过滤器上，并且流体通过过滤器并进入吸收材料，从而在过滤器或膜的结构的表面上或内部分离晶体。 然后晶体可用于染色，其将显示晶体的确定组分。 该装置还可以包括用于抑制从吸收材料向过滤器回流的构件。本发明还提供了用于检测和鉴定可能存在于生物流体中的晶体的方法。 所述方法包括以下步骤：将过滤器或膜的结构表面上或结晶内的晶体分离，并将晶体与特定于晶体类型的确定组分的指示剂接触。还提供了包括装置的试剂盒 本发明的方法，以及进行本发明方法所需的试剂和其他材料。

公开(公告)号：US5753456A

公开(公告)日：1998-05-19

申请号：US394315

申请日：1995-02-22

申请人： Ali Naqui ,  Mark W. Pierson ,  Thomas R. Weschler ,  Stephen C. Wardlaw ,  Michael P. Finnerty ,  Charles R. Carpenter

发明人： Ali Naqui ,  Mark W. Pierson ,  Thomas R. Weschler ,  Stephen C. Wardlaw ,  Michael P. Finnerty ,  Charles R. Carpenter

IPC分类号： G01N33/487 ,  B01L3/00 ,  B65D75/32 ,  B65D75/34 ,  B65D75/36 ,  B65D75/40 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/02

CPC分类号： B01L3/5085 ,  B01L3/505 ,  B65D75/324 ,  C12Q1/04 ,  G01N2333/245 ,  Y10S435/81 ,  Y10S435/975

摘要： An article adapted for holding a liquid sample for quantification of biological material in the liquid sample. The article includes a bag having an upper surface sheet and a lower surface sheet enclosing a volume therebetween. The bag has an upper opening through which the liquid sample can be poured into the volume in the bag. The bag also has a plurality of partitions configured to separate one or more portions of adequate sample in the bag. Also provided is a passage through which a liquid sample can be distributed throughout the volume in the bag. The bag is made of material which can be caused to form discreet non-permeable compartments for holding separate aliquots of the liquid sample.

摘要翻译： 一种用于容纳用于定量液体样品中的生物材料的液体样品的制品。 该物品包括具有上表面片和在其间包围体积的下表面片的袋。 该袋具有上部开口，液体样品可以通过该上部开口倒入袋中的体积中。 该袋还具有多个分隔件，其被配置为分离袋中足够样品的一个或多个部分。 还提供了一种通道，液体样品可通过该通道分布在袋中的整个体积中。 该袋由材料制成，其可以形成离散的非渗透性隔室，用于保持液体样品的单独等分试样。

公开(公告)号：US06551842B1

公开(公告)日：2003-04-22

申请号：US09525151

申请日：2000-03-14

申请人： Charles R. Carpenter

发明人： Charles R. Carpenter

IPC分类号： G01N33533

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S435/91

摘要： A disposable, dry chemistry analytical system is disclosed which is broadly useful for the detection of a variety of analytes present in biological fluids such as whole blood, serum, plasma, urine and cerebral spinal fluid. The invention discloses the use of the reaction interface that forms between two liquids converging from opposite directions within a bibulous material. The discovery comprises a significant improvement over prior art disposable, analytical reagent systems in that the detectable reactant zone is visually distinct and separate from the unreacted reagents allowing for the use of reaction indicators exhibiting only minor changes as well as extremely high concentrations of reactants. In addition, staged, multiple reagents can be incorporated. Whole blood can be used as a sample without the need for separate cell separating materials. Finally, the invention is useful for the detection of analytes in a broad variety of materials such as milk, environmental samples, and other samples containing target analytes.

摘要翻译： 公开了一次性干燥化学分析系统，其广泛用于检测生物流体如全血，血清，血浆，尿液和脑脊液中存在的各种分析物。 本发明公开了在吸水材料内从两相反方向汇合的两种液体之间形成的反应界面的应用。 该发现包括对现有技术的一次性分析试剂系统的显着改进，因为可检测的反应物区域在视觉上是不同的，并且与未反应的试剂分离，允许使用仅显示微小变化的反应指示剂以及极高浓度的反应物。 此外，可以并入分阶段的多种试剂。 全血可用作样品，不需要单独的细胞分离材料。 最后，本发明可用于检测多种材料中的分析物，例如牛奶，环境样品和含有目标分析物的其它样品。

公开(公告)号：US06511814B1

公开(公告)日：2003-01-28

申请号：US09439024

申请日：1999-11-12

申请人： Charles R. Carpenter

发明人： Charles R. Carpenter

IPC分类号： G01N33567

CPC分类号： G01N33/523 ,  G01N33/80 ,  Y10S435/81 ,  Y10S435/97

摘要： A disposable, dry chemistry analytical system is disclosed which is broadly useful for the detection of a variety of analytes present in biological fluids such as whole blood, serum, plasma, urine and cerebral spinal fluid. The invention discloses the use of the reaction interface that forms between two liquids converging from opposite directions within a bibulous material. The discovery comprises a significant improvement over prior art disposable, analytical reagent systems in that the detectable reactant zone is visually distinct and separate from the unreacted reagents allowing for the use of reaction indicators exhibiting only minor changes as well as extremely high concentrations of reactants. In addition, staged, multiple reagents can be incorporated. Whole blood can be used as a sample without the need for separate cell separating materials. Finally, the invention is useful for the detection of analytes in a broad variety of materials such as milk, environmental samples, and other samples containing target analytes.

公开(公告)号：US4483922A

公开(公告)日：1984-11-20

申请号：US378441

申请日：1982-05-14

申请人： Charles R. Carpenter ,  Robert H. Dodge ,  Kenneth A. Rosanoff

发明人： Charles R. Carpenter ,  Robert H. Dodge ,  Kenneth A. Rosanoff

IPC分类号： C12N9/99 ,  G01N33/96 ,  C12Q1/00 ,  C12Q1/32 ,  C12Q1/42 ,  C12Q1/44

CPC分类号： G01N33/96 ,  C12N9/0006 ,  C12N9/104 ,  C12N9/16 ,  C12N9/50 ,  C12N9/99 ,  C12Y101/01027 ,  C12Y203/02002 ,  C12Y207/03002 ,  C12Y301/03001 ,  G01N2333/595 ,  G01N2333/62 ,  G01N2333/695 ,  G01N2496/05

摘要： A method for inactivating enzymes, particularly enzymes found in human serum. The method comprises reacting the enzyme in a suitable medium, e.g. human serum, with an inactivating amount of peracetic acid. In the preferred embodiment the treated serum is subsequently neutralized and dialyzed. Preferably, the serums are used as standards for calibration or as a control in assay kits, e.g. RIA, and EIA or enzyme kits, for the determination of specific ligands and enzymes.

摘要翻译： 灭活酶的方法，特别是在人血清中发现的酶。 该方法包括使酶在合适的培养基中，例如， 人血清，具有灭活量的过乙酸。 在优选的实施方案中，随后中和和透析处理的血清。 优选地，将血清用作校准的标准物或作为测定试剂盒中的对照，例如。 RIA和EIA或酶试剂盒，用于测定特异性配体和酶。