公开(公告)号：US5585261A

公开(公告)日：1996-12-17

申请号：US328739

申请日：1994-10-25

申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

IPC分类号： C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  C12P19/02 ,  C12N9/86 ,  C12N1/20

CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield.

摘要翻译： 公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。

公开(公告)号：US06423834B1

公开(公告)日：2002-07-23

申请号：US09247164

申请日：1999-02-09

申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

IPC分类号： C07H1500

CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.

摘要翻译： 公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。 另外，保护形式的KDO醛缩酶产物，例如， 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

公开(公告)号：US5869316A

公开(公告)日：1999-02-09

申请号：US767182

申请日：1996-12-16

申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

IPC分类号： C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  C12P19/02 ,  C12N1/20

CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

摘要： Aureobacterium barkeri strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.

摘要翻译： 公开了分离自巴斯德氏菌杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。 另外，保护形式的KDO醛缩酶产物，例如， 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

公开(公告)号：US5358859A

公开(公告)日：1994-10-25

申请号：US993140

申请日：1992-12-18

申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

IPC分类号： C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  C12P19/02

CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The DKDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.

摘要翻译： 公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）KDO醛缩酶（EC 4.1.2.23）。 进一步公开了DKDO醛缩酶对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。 另外，保护形式的KDO醛缩酶产物，例如， 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

公开(公告)号：US06319695B1

公开(公告)日：2001-11-20

申请号：US07961076

申请日：1992-10-14

申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen ,  Kun-Chin Liu

发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen ,  Kun-Chin Liu

IPC分类号： C12P1918

CPC分类号： C07H5/02 ,  C07H3/06 ,  C07H11/04 ,  C07H13/04 ,  C07H13/08 ,  C07H15/18 ,  C07H19/20 ,  C12P19/18 ,  C12P19/26

摘要： This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.

摘要翻译： 本发明考虑了碳水化合物，特别是岩藻糖基化碳水化合物的酶促生产的改进方法。 还考虑了使用化学和酶学方法改进的1或2-磷酸糖基酯的合成。 然后将磷酸化糖苷用于产生糖核苷酸，其又被用作供体糖用于受体碳水化合物的糖基化。 本文特别优选使用所公开的岩藻糖基化方法。

公开(公告)号：US5593887A

公开(公告)日：1997-01-14

申请号：US476685

申请日：1995-06-07

申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

IPC分类号： C07H3/06 ,  C07H7/027 ,  C07H13/06 ,  C12N1/21 ,  C12N9/12 ,  C12N15/09 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26 ,  C12R1/19 ,  C07H5/04 ,  C12N15/00

CPC分类号： C12N9/1241 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

摘要： Oligosacaharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.

摘要翻译： 公开了作为底物的寡糖沙必利化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。 还公开了糖基化的方法。 用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

公开(公告)号：US5385833A

公开(公告)日：1995-01-31

申请号：US841718

申请日：1992-02-26

申请人： Curt W. Bradshaw ,  Chi-Huey Wong ,  Gwo-Jenn Shen

发明人： Curt W. Bradshaw ,  Chi-Huey Wong ,  Gwo-Jenn Shen

IPC分类号： C12N9/04 ,  C12P7/02 ,  C12N1/20 ,  C12P7/00 ,  C12R1/38

CPC分类号： C12N9/0006 ,  C12P7/02 ,  C12R1/38 ,  Y10S435/874

摘要： The present invention provides a substantially pure culture of Pseudomonas sp. strain PED having the ATCC designation 49794. Processes for making R-configured alcohols and for transferring a hydride ion from an R-configured alcohol to the pro-R face of NAD using PED alcohol dehydrogenase isolated and purified from Pseudomonas sp. strain PED are also provided.

摘要翻译： 本发明提供了基本上纯的假单胞菌属培养物。 具有ATCC命名为49794的菌株PED。使用从假单胞菌属（Pseudomonas sp。）分离和纯化的PED醇脱氢酶制备R-构型的醇并将氢离子从R构型的醇转移到NAD的前R面的方法。 还提供了菌株PED。

公开(公告)号：US07335500B2

公开(公告)日：2008-02-26

申请号：US09992680

申请日：2001-11-19

申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen ,  Kun-Chin Liu

发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen ,  Kun-Chin Liu

IPC分类号： C12P19/22

CPC分类号： C07H5/02 ,  C07H3/06 ,  C07H11/04 ,  C07H13/04 ,  C07H13/08 ,  C07H15/18 ,  C07H19/20 ,  C12P19/18 ,  C12P19/26

摘要： This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.

摘要翻译： 本发明考虑了碳水化合物，特别是岩藻糖基化碳水化合物的酶促生产的改进方法。 还考虑了使用化学和酶学方法改进的1或2-磷酸糖基酯的合成。 然后将磷酸化糖苷用于产生糖核苷酸，其又被用作供体糖用于受体碳水化合物的糖基化。 本文特别优选使用所公开的岩藻糖基化方法。

公开(公告)号：US5759823A

公开(公告)日：1998-06-02

申请号：US472877

申请日：1995-06-07

申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

IPC分类号： C07H3/06 ,  C07H7/027 ,  C07H13/06 ,  C12N1/21 ,  C12N9/12 ,  C12N15/09 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26 ,  C12R1/19 ,  C12P19/12

CPC分类号： C12N9/1241 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.

摘要翻译： 公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。 还公开了糖基化的方法。 用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

公开(公告)号：US5461143A

公开(公告)日：1995-10-24

申请号：US219242

申请日：1994-03-29

申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

IPC分类号： C12N9/12 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26 ,  C07H5/04 ,  C07H5/10

CPC分类号： C12N9/1241 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.

摘要翻译： 公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。 还公开了糖基化的方法。 用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。