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15 条记录 (耗时 0.037 秒)

    Method and composition for synthesizing sialylated glycosyl compounds
    1.
    发明授权
    Method and composition for synthesizing sialylated glycosyl compounds 失效
    合成唾液酸化糖基化合物的方法和组成

    公开(公告)号：US5278299A

    公开(公告)日：1994-01-11

    申请号：US670701

    申请日：1991-03-18

    申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

    IPC分类号： C12N1/21 ,  C12N9/12 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26 ,  C07H1/00 ,  C12N9/24

    CPC分类号： C12N9/1241 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

    摘要： The present invention provides a method for synthesizing a sialylated glycosyl compound comprising reacting in the presence of each other a sialic acid, a glycosyl compound, a CMP-sialic acid regenerating system, a pyrophosphate scavenger and catalytic amounts of a CMP-sialic acid synthetase and a sialyl transferase having substrate specificity for the glycosyl compound. The present invention also provides a composition for sialylating glycosyl compounds comprising a sialic acid, CMP-sialic acid regenerating system, a pyrophosphate scavenger and a catalytic amount of CMP-sialic acid synthetase. The composition can further comprise an aqueous solvent having a suitable buffer and enzyme cofactors as well as a catalytic amount of a sialyl transferase having substrate specificity for the glycosyl compound. A phagemid-transformed E. coli. that overproduces CMP-sialic acid synthetase is also disclosed.

    摘要翻译： 本发明提供了合成唾液酸化糖基化合物的方法，包括在唾液酸，糖基化合物，CMP-唾液酸再生系统，焦磷酸盐清除剂和催化量的CMP-唾液酸合成酶的存在下彼此反应，以及 具有对糖基化合物具有底物特异性的唾液酸转移酶。 本发明还提供了用于唾液酸化糖基化合物的组合物，其包含唾液酸，CMP-唾液酸再生系统，焦磷酸盐清除剂和催化量的CMP-唾液酸合成酶。 组合物还可以包含具有合适的缓冲液和酶辅因子的水性溶剂以及催化量的对糖基化合物具有底物特异性的唾液酸转移酶。 噬菌粒转化的大肠杆菌。 也公开了过度生产CMP-唾液酸合成酶。

    Oligosaccharide enzyme substrates and inhibitors: methods and compositions
    2.
    发明授权
    Oligosaccharide enzyme substrates and inhibitors: methods and compositions 失效
    寡糖酶底物和抑制剂：方法和组合物

    公开(公告)号：US06168934A

    公开(公告)日：2001-01-02

    申请号：US09072958

    申请日：1998-05-05

    申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

    IPC分类号： C12P1918

    CPC分类号： C12N9/1241 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

    摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.

    摘要翻译： 公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。 还公开了糖基化的方法。 用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

    KDO aldolase and condensation reactions employed therewith
    8.
    发明授权
    KDO aldolase and condensation reactions employed therewith 失效
    KDO醛缩酶和与其一起使用的缩合反应

    公开(公告)号：US06423834B1

    公开(公告)日：2002-07-23

    申请号：US09247164

    申请日：1999-02-09

    申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    IPC分类号： C07H1500

    CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

    摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.

    摘要翻译： 公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。 另外，保护形式的KDO醛缩酶产物，例如， 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

    Biologically pure culture of aureobacterium barkeri KDO-37-2
    10.
    发明授权
    Biologically pure culture of aureobacterium barkeri KDO-37-2 失效
    生物纯培养的巴氏杆菌KDO-37-2

    公开(公告)号：US5869316A

    公开(公告)日：1999-02-09

    申请号：US767182

    申请日：1996-12-16

    申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    IPC分类号： C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  C12P19/02 ,  C12N1/20

    CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

    摘要： Aureobacterium barkeri strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.

    摘要翻译： 公开了分离自巴斯德氏菌杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。 另外，保护形式的KDO醛缩酶产物，例如， 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。