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180 条记录 (耗时 0.038 秒)

    KDO aldolase
    4.
    发明授权
    KDO aldolase 失效
    KDO醛缩酶

    公开(公告)号：US5585261A

    公开(公告)日：1996-12-17

    申请号：US328739

    申请日：1994-10-25

    申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    IPC分类号： C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  C12P19/02 ,  C12N9/86 ,  C12N1/20

    CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

    摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield.

    摘要翻译： 公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。

    Oligosaccharide enzyme substrates and inhibitors: methods and compositions
    8.
    发明授权
    Oligosaccharide enzyme substrates and inhibitors: methods and compositions 失效
    寡糖酶底物和抑制剂：方法和组合物

    公开(公告)号：US06168934A

    公开(公告)日：2001-01-02

    申请号：US09072958

    申请日：1998-05-05

    申请人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Yoshitaka Ichikawa ,  Gwo-Jenn Shen

    IPC分类号： C12P1918

    CPC分类号： C12N9/1241 ,  C12P19/04 ,  C12P19/18 ,  C12P19/26

    摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.

    摘要翻译： 公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。 还公开了糖基化的方法。 用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

    KDO aldolase and condensation reactions employed therewith
    9.
    发明授权
    KDO aldolase and condensation reactions employed therewith 失效
    KDO醛缩酶和与其一起使用的缩合反应

    公开(公告)号：US06423834B1

    公开(公告)日：2002-07-23

    申请号：US09247164

    申请日：1999-02-09

    申请人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    发明人： Chi-Huey Wong ,  Takeshi Sugai ,  Gwo-Jenn Shen

    IPC分类号： C07H1500

    CPC分类号： C12P19/02 ,  C07H7/027 ,  C12N9/88 ,  C12P7/58 ,  C12P9/00 ,  Y10S435/822

    摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.

    摘要翻译： 公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。 进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。 特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。 另外，保护形式的KDO醛缩酶产物，例如， 六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。