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    Superoxide dismutase fusion polypeptides for expression of mammalian proteins
    6.
    发明授权
    Superoxide dismutase fusion polypeptides for expression of mammalian proteins 失效
    用于表达哺乳动物蛋白质的超氧化物歧化酶融合多肽

    公开(公告)号：US5342921A

    公开(公告)日：1994-08-30

    申请号：US680046

    申请日：1991-03-29

    申请人： Lawrence S. Cousens ,  Patricia A. Tekamp-Olson ,  Jeffrey R. Shuster ,  James P. Merryweather

    发明人： Lawrence S. Cousens ,  Patricia A. Tekamp-Olson ,  Jeffrey R. Shuster ,  James P. Merryweather

    IPC分类号： C07K14/16 ,  C07K14/62 ,  C07K14/65 ,  C12N9/02 ,  C12N15/81 ,  C07K3/00 ,  C07K13/00

    CPC分类号： C07K14/005 ,  C07K14/62 ,  C07K14/65 ,  C12N15/81 ,  C12N9/0089 ,  C12Y115/01001 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/50 ,  C07K2319/75 ,  C12N2740/16222 ,  Y10S435/814

    摘要： Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two polypeptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield.The S. cerevisiae strain 2150-2-3 (pYASI1) was deposited at the A.T.C.C. on Feb. 27, 1985 and given accession no. 20745.The S. cerevisiae strain AB110 (pYLUIGF2-14) was deposited at the A.T.C.C. on Mar. 19, 1986 and given accession no. 20796.

    摘要翻译： 提供了新的方法和组合物用于提高真菌中异源蛋白质的产量。 所述方法和组合物涉及使用融合序列，所述融合序列涉及编码在相对大量产生的异源产物的序列，作为在开放阅读框中与第二序列融合的宿主中的稳定多肽，其中先前的序列编码不同的异源多肽，其中两个 多肽通过可选择性切割的连接键连接。 特别地，编码超氧化物歧化酶的序列在酵母表达载体的超氧化物歧化酶的任一末端与活性启动子的转录控制和导入酵母宿主和宿主生长的载体连接至另一种目的多肽。 以这种方式获得高产量的融合产物，其中可以选择性地切割融合产物以便以高产率产生超氧化物歧化酶和另一种多肽。 酿酒酵母菌株2150-2-3（pYASI1）保藏在A.T.C.C. 1985年2月27日， 酿酒酵母菌株AB110（pYLUIGF2-14）保藏在A.T.C.C. 1986年3月19日，加拿大号。 20796。

    Methods for Detection of Fungal Disease
    7.
    发明申请
    Methods for Detection of Fungal Disease 审中-公开
    真菌病检测方法

    公开(公告)号：US20080254496A1

    公开(公告)日：2008-10-16

    申请号：US12088107

    申请日：2006-09-26

    申请人： Jeffrey R. Shuster

    发明人： Jeffrey R. Shuster

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/04 ,  C12Q1/26 ,  G01N2333/37

    摘要： The present invention describes the use of enzymes having D-arabinitol oxidase activity with the concomitant generation of hydrogen peroxide for the diagnosis of fungal infections of humans and other organisms, and for the detection of fungal organisms that are present on or in surfaces or permeable materials. The present invention also describes methods and kits for the detection of fungi, and the diagnosis of fungal infections based upon D-arabinitol oxidase activity. The present invention also provides a new biochemical activity for gene products encoded by bacterial genes previously identified only with putative function. Activity of these gene products includes the ability to oxidize D-arabinitol with the concomitant generation of hydrogen peroxide.

    摘要翻译： 本发明描述了具有D-阿拉伯糖醇氧化酶活性的酶与伴随产生过氧化氢的酶用于诊断人和其他生物体的真菌感染的用途，以及用于检测存在于或表面上的真菌生物或可渗透材料 。 本发明还描述了用于检测真菌的方法和试剂盒，以及基于D-阿拉伯糖醇氧化酶活性的真菌感染的诊断。 本发明还提供了由以前仅用推定功能鉴定的细菌基因编码的基因产物的新的生化活性。 这些基因产物的活性包括伴随产生过氧化氢氧化D-阿拉伯糖醇的能力。

    Morphological mutants of filamentous fungi
    9.
    发明授权
    Morphological mutants of filamentous fungi 有权
    丝状真菌的形态突变体

    公开(公告)号：US06184026B2

    公开(公告)日：2001-02-06

    申请号：US09405564

    申请日：1999-09-24

    申请人： Jeffrey R. Shuster ,  John C. Royer

    发明人： Jeffrey R. Shuster ,  John C. Royer

    IPC分类号： C12N115

    CPC分类号： C12N9/2437 ,  C12N9/20 ,  C12N15/01 ,  C12N15/102 ,  C12N15/80 ,  C12R1/69 ,  C12R1/77

    摘要： The present invention relates to methods of obtaining a mutant cell from a filamentous fungal parent cell, comprising: (a) obtaining mutant cells of the parent cell; (b) identifying the mutant cell which exhibits a more restricted colonial phenotype and/or a more extensive hyphal branching than the parent cell; and (c) identifying the mutant cell which has an improved property for production of a heterologous polypeptide than the parent cell, when the mutant and parent cells are cultured under the same conditions.

    摘要翻译： 本发明涉及从丝状真菌亲本细胞获得突变细胞的方法，包括：（a）获得亲本细胞的突变细胞; （b）鉴定突变细胞，其表现出比母体细胞更加有限的殖民地表型和/或更广泛的菌丝分枝; 和（c）当在相同条件下培养突变体和亲代细胞时，鉴定出与亲本细胞相比具有改进的产生异源多肽性质的突变细胞。