公开(公告)号：US11807666B2

公开(公告)日：2023-11-07

申请号：US18059929

申请日：2022-11-29

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01J41/20 ,  B01D15/36 ,  B01D15/38 ,  A61K38/36 ,  A61K38/48 ,  A61K35/16 ,  C12N9/64 ,  B01D15/34 ,  C07K14/47 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  A61K38/00

CPC classification number: C07K1/18 ,  A61K35/16 ,  A61K38/36 ,  A61K38/4846 ,  B01D15/34 ,  B01D15/363 ,  B01D15/3847 ,  B01J41/20 ,  C07K14/472 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  C12N9/644 ,  C12N9/6424 ,  C12Y304/21022 ,  A61K38/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US11542300B2

公开(公告)日：2023-01-03

申请号：US15564232

申请日：2016-04-04

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01J41/20 ,  B01D15/36 ,  B01D15/38 ,  A61K38/36 ,  A61K38/48 ,  A61K35/16 ,  C12N9/64 ,  B01D15/34 ,  C07K14/47 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00 ,  A61K38/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of plasmaproteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US20220111310A1

公开(公告)日：2022-04-14

申请号：US17544700

申请日：2021-12-07

Applicant: Cytiva Bioprocess R&D AB

Inventor： Karol Maciej Lacki ,  Hans Blom ,  Helena Skoglar ,  Linus Laurin

IPC: B01D15/38 ,  B01J20/24 ,  B01J20/286 ,  B01J20/32 ,  C07K1/22 ,  B01J20/28 ,  C07K16/06

Abstract: The invention discloses a separation matrix comprised of porous spherical particles to which antibody-binding protein ligands have been covalently immobilized, wherein the density of said ligands is in the range of 10.5-15 mg/ml and the volume-weighted median diameter of said particles is in the range of 30-55 μm.

公开(公告)号：US20230091082A1

公开(公告)日：2023-03-23

申请号：US18059929

申请日：2022-11-29

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01J41/20 ,  B01D15/36 ,  B01D15/38 ,  A61K38/36 ,  A61K38/48 ,  A61K35/16 ,  C12N9/64 ,  B01D15/34 ,  C07K14/47 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US11285460B2

公开(公告)日：2022-03-29

申请号：US15120845

申请日：2015-02-18

Applicant: Cytiva BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Karolina Busson ,  Karol Lacki ,  Bjorn Noren ,  Helena Skoglar

IPC: B01D15/12 ,  B01D15/20 ,  B01D15/30 ,  B01D15/36 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  C07K1/22 ,  C07K14/765 ,  C07K14/77 ,  C12N1/20

Abstract: The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.