公开(公告)号：US20190002937A1

公开(公告)日：2019-01-03

申请号：US16064552

申请日：2016-12-20

申请人： Danisco US Inc.

发明人： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC分类号： C12P19/02 ,  C12P19/14 ,  C12N9/34 ,  C12N9/30

摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US09365871B2

公开(公告)日：2016-06-14

申请号：US14561085

申请日：2014-12-04

申请人： DANISCO US INC.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P19/14 ,  C12C7/04 ,  C12P7/14

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US08945889B2

公开(公告)日：2015-02-03

申请号：US13888303

申请日：2013-05-06

申请人： Danisco US Inc.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P7/36 ,  C12P7/02 ,  C12P19/14 ,  C12C7/04

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmyl）提供。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20190010473A1

公开(公告)日：2019-01-10

申请号：US16064518

申请日：2016-12-20

申请人： Danisco US Inc.

发明人： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC分类号： C12N9/30 ,  C12P19/14 ,  C12P7/06 ,  C12N9/34 ,  C12P19/02

摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US20190002854A1

公开(公告)日：2019-01-03

申请号：US16064577

申请日：2016-12-20

申请人： Bart C. KOOPS ,  Paula Johanna Maria TEUNISSEN ,  Marco VAN BRUSSEL-ZWIJNEN ,  Martijn SCHEFFERS ,  Kees-Jan GUIJT ,  Zhengzheng ZOU ,  Zhongmei TANG ,  Zhen QIAN ,  Jing GE ,  Zhenghong ZHANG ,  Danisco US Inc.

发明人： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC分类号： C12N9/34 ,  C12P7/06 ,  C12P19/12 ,  C12P19/02

摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US20130323798A1

公开(公告)日：2013-12-05

申请号：US13888303

申请日：2013-05-06

申请人： Danisco US Inc.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P19/14 ,  C12C7/04

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 从曲霉菌（AcAmyl）提供真菌α-淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20230340443A1

公开(公告)日：2023-10-26

申请号：US18151122

申请日：2023-01-06

申请人： Danisco US Inc.

发明人： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC分类号： C12N9/30 ,  C12P19/02 ,  C12P19/14 ,  C12N9/34 ,  C12P7/06

CPC分类号： C12N9/242 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01001 ,  C12Y302/01003 ,  C12N9/2428 ,  C12P7/06

摘要： Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US20150152442A1

公开(公告)日：2015-06-04

申请号：US14561085

申请日：2014-12-04

申请人： DANISCO US INC.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P7/14 ,  C12C7/04

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。