公开(公告)号：US09365871B2

公开(公告)日：2016-06-14

申请号：US14561085

申请日：2014-12-04

Applicant: DANISCO US INC.

Inventor： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC: C12P19/14 ,  C12C7/04 ,  C12P7/14

CPC classification number: C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20150218606A1

公开(公告)日：2015-08-06

申请号：US14419902

申请日：2013-08-13

Applicant: DANISCO US INC.

Inventor： Marco van Brussel-Zwijnen ,  Jacquelyn A. Huitink ,  Martijn Silvan Scheffers ,  Paula Johanna Maria Teunissen ,  Casper Vroemen

IPC: C12P19/02 ,  C12N9/30 ,  C12P19/16 ,  A21D8/04 ,  C11D3/386 ,  C12C5/00 ,  C12C11/00 ,  C12N9/44 ,  C12P7/14

CPC classification number: C12P19/02 ,  A21D8/042 ,  C11D3/386 ,  C11D3/38636 ,  C12C5/004 ,  C12C11/003 ,  C12N9/242 ,  C12N9/2457 ,  C12P7/06 ,  C12P7/14 ,  C12P19/14 ,  C12P19/16 ,  C12Y302/01001 ,  C12Y302/01041 ,  D06L1/14 ,  D06L4/40 ,  Y02E50/17

Abstract: A fungal alpha amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75 C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 从曲霉菌（AcAmyl）提供真菌α淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，使得酶可在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为间歇方法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α淀粉酶或葡糖淀粉酶。 AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化，与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20150152442A1

公开(公告)日：2015-06-04

申请号：US14561085

申请日：2014-12-04

Applicant: DANISCO US INC.

Inventor： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC: C12P7/14 ,  C12C7/04

CPC classification number: C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US09765374B2

公开(公告)日：2017-09-19

申请号：US14649173

申请日：2013-12-05

Applicant: Danisco US Inc.

Inventor： Ling Hua ,  Martijn Scheffers ,  Marco Van Brussel ,  Casper Vroemen ,  Bo Zhang ,  Zhengong Zhang

IPC: C12P7/06 ,  C12P19/14 ,  A21D8/04 ,  C11D3/386 ,  D06L1/06 ,  C12C5/00 ,  C12N9/30 ,  C12N9/44 ,  C12C11/00 ,  C12P19/02 ,  A23L7/104

CPC classification number: C12P19/14 ,  A21D8/042 ,  A23L7/107 ,  A23V2002/00 ,  C11D3/386 ,  C12C5/004 ,  C12C11/003 ,  C12N9/242 ,  C12N9/246 ,  C12P7/06 ,  C12P19/02 ,  C12Y302/01001 ,  C12Y302/01068 ,  D06L1/06 ,  Y02E50/17

Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

公开(公告)号：US09540669B2

公开(公告)日：2017-01-10

申请号：US14419906

申请日：2013-08-12

Applicant: DANISCO US INC.

Inventor： Su Yin Marina Chow ,  Thomas P. Graycar ,  Jacquelyn A. Huitink ,  Casper Vroemen ,  David L. Wong

IPC: C12N9/34 ,  C12P19/20 ,  C12P19/14 ,  C12P19/02 ,  C12P7/10

CPC classification number: C12P19/20 ,  C12N9/2428 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01003 ,  Y02E50/16 ,  Y02P20/52

Abstract: Embodiments of the present disclosure relate to Trichoderma reesei glucoamylase (TrGA) variants having improved properties (e.g., improved thermostability, improved specific activity, and/or resistant to oxidation-related activity loss). Also provided are compositions comprising variant glucoamylases. These compositions are useful in various starch process applications.

Abstract translation: 本公开的实施方案涉及具有改善的性质（例如改善的热稳定性，改善的比活性和/或耐氧化相关活性损失）的里氏木霉葡糖淀粉酶（TrGA）变体。 还提供了包含变体葡糖淀粉酶的组合物。 这些组合物可用于各种淀粉工艺应用。

公开(公告)号：US20140227754A1

公开(公告)日：2014-08-14

申请号：US14172790

申请日：2014-02-04

Applicant: Danisco US Inc.

Inventor： Wolfgang Aehle ,  Richard R. Bott ,  Martijn Scheffers ,  Pieter van Solingen ,  Casper Vroemen

IPC: C12N9/34 ,  C12P7/06

CPC classification number: C12N9/2428 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/17

Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

Abstract translation: 本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。 特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。 本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。

公开(公告)号：US20130344542A1

公开(公告)日：2013-12-26

申请号：US13899057

申请日：2013-05-21

Applicant: DANISCO US INC.

Inventor： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, JR. ,  Brian E. Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

IPC: C12N9/28

CPC classification number: C12N9/2417 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L29/06

Abstract: Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

Abstract translation: 描述了具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质的亲本α-淀粉酶的变体（突变体）及其使用方法。

公开(公告)号：US20130323798A1

公开(公告)日：2013-12-05

申请号：US13888303

申请日：2013-05-06

Applicant: Danisco US Inc.

Inventor： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC: C12P19/14 ,  C12C7/04

CPC classification number: C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 从曲霉菌（AcAmyl）提供真菌α-淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US09290753B2

公开(公告)日：2016-03-22

申请号：US14172790

申请日：2014-02-04

Applicant: Danisco US Inc.

Inventor： Wolfgang Aehle ,  Richard R. Bott ,  Martijn Scheffers ,  Pieter van Solingen ,  Casper Vroemen

IPC: C12N15/56 ,  C12N9/34 ,  C12P7/06 ,  C12P19/14 ,  C12P19/02

CPC classification number: C12N9/2428 ,  C12P7/06 ,  C12P19/02 ,  C12P19/14 ,  Y02E50/17

Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.

公开(公告)号：US20160010128A1

公开(公告)日：2016-01-14

申请号：US14649807

申请日：2013-12-06

Applicant: DANISCO US INC.

Inventor： Jing Ge ,  Ling Hua ,  Martijn Scheffers ,  Marco Van Brussel ,  Casper Vroemen ,  Bo Zhang

IPC: C12P19/14 ,  C12P19/02 ,  C12P7/14

CPC classification number: C12P19/14 ,  A21D8/042 ,  A23L2/382 ,  A23L7/107 ,  A23L29/35 ,  C11D3/386 ,  C12C5/004 ,  C12C7/04 ,  C12C7/16 ,  C12C11/003 ,  C12C12/04 ,  C12N9/242 ,  C12P7/06 ,  C12P7/14 ,  C12P19/02 ,  C12P2203/00 ,  C13K1/06 ,  C13K11/00 ,  D06L1/06 ,  Y02E50/17

Abstract: A fungal alpha-amylase is provided from Aspergillus terreus (AtAmyl). AtAmyl has an optimal pH of 4.5 and is operable at 30 75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 由Aspergillus terreus（AtAmyl）提供真菌α-淀粉酶。 AtAmyl具有4.5的最佳pH，并且在30±75℃下可操作，使酶在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α-淀粉酶催化的糖化产物相比，AtAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。