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公开(公告)号：US09765374B2
公开(公告)日：2017-09-19
申请号：US14649173
申请日：2013-12-05
Applicant: Danisco US Inc.
Inventor： Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhengong Zhang
IPC: C12P7/06 , C12P19/14 , A21D8/04 , C11D3/386 , D06L1/06 , C12C5/00 , C12N9/30 , C12N9/44 , C12C11/00 , C12P19/02 , A23L7/104
CPC classification number: C12P19/14 , A21D8/042 , A23L7/107 , A23V2002/00 , C11D3/386 , C12C5/004 , C12C11/003 , C12N9/242 , C12N9/246 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01068 , D06L1/06 , Y02E50/17
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
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2.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION 有权使用来自青蒿素的ALPHA-AMYLASE进行分离的方法公开(公告)号：US20130323798A1
公开(公告)日：2013-12-05
申请号：US13888303
申请日：2013-05-06
Applicant: Danisco US Inc.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 从曲霉菌（AcAmyl）提供真菌α-淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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公开(公告)号：US10767207B2
公开(公告)日：2020-09-08
申请号：US16281989
申请日：2019-02-21
Applicant: DANISCO US INC
Inventor： Jing Ge , Ling Hua , Sung Ho Lee , Jalsen Li , Jayarama K Shetty , Zhongmei Tang , Bo Zhang , Kun Zhong
Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
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公开(公告)号：US09890374B2
公开(公告)日：2018-02-13
申请号：US15215844
申请日：2016-07-21
Applicant: DANISCO US INC
Inventor： Jing Ge , Ling Hua , Ayrookaran J. Poulose
CPC classification number: C12N9/88 , B01D53/62 , B01D53/84 , B01D2251/95 , B01D2258/0283 , C02F3/342 , C12N11/14 , C12Y402/01001 , Y02E50/343
Abstract: The present compositions and methods relate to a thermostable carbonic anhydrases, polynucleotides encoding the carbonic anhydrase, and methods of make and/or use thereof. Formulations containing the carbonic anhydrase are suitable for use in extracting carbon dioxide.
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5.发明申请公开(公告)号：US20160160199A1
公开(公告)日：2016-06-09
申请号：US14907718
申请日：2014-09-19
Applicant: DANISCO US INC.
Inventor： Guoqing Liu , Ling Hua , Zhen Qian , Danfeng Song , Zhongmei Tang , Xie Zhiyong , Bo Zhang , Zhengzheng Zou , Wei Xu
Abstract: Disclosed are compositions and methods relating to alpha-amylase from Exiguobacterium. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and and for baking and brewing.
Abstract translation: 公开了与来自Exiguobacterium的α-淀粉酶相关的组合物和方法。 组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它用途中的淀粉污渍，用于织物加工（例如脱浆），动物饲料中用于改善消化率，以及用于烘烤和 酿造。
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Patent Agency Ranking
公开(公告)号：US20160130571A1
公开(公告)日：2016-05-12
申请号：US14898700
申请日：2014-05-15
Applicant: Danisco US Inc.
Inventor： Ling Hua , Marc Kolkman , Zhen Qian , Bo Zhang
CPC classification number: C12N9/2417 , A23K20/189 , A23L29/35 , C11D3/386 , C11D3/38681 , C12N9/2414 , C12Y302/01001
Abstract: Disclosed are compositions and methods relating to an alpha-amylase from a Bacillaceae family member. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing.
Abstract translation: 公开了与来自芽孢杆菌科家族成员的α-淀粉酶相关的组合物和方法。 组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它应用中的淀粉污渍，用于织物加工（例如脱浆），用于改善消化率的动物饲料中，以及烘烤和酿造 。
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7.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION 审中-公开使用来自青蒿素的ALPHA-AMYLASE进行分离的方法公开(公告)号：US20150152442A1
公开(公告)日：2015-06-04
申请号：US14561085
申请日：2014-12-04
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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8.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND PULLULANASE FOR SACCHARIFICATION 审中-公开使用来自ASPERGILLUS FUMIGATUS和PULLULANASE的ALPHA-AMYLASE进行鉴定的方法公开(公告)号：US20160040202A1
公开(公告)日：2016-02-11
申请号：US14649170
申请日：2013-12-05
Applicant: DANISCO US INC.
Inventor： Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhenghong Zhang
CPC classification number: C12P19/02 , A21D8/042 , A23L2/382 , C11D3/38636 , C12C5/004 , C12C7/04 , C12C7/16 , C12C11/003 , C12C12/04 , C12G3/02 , C12N9/242 , C12P7/14 , C12P19/14 , C12Y302/01001 , C13K1/06 , D06L1/06
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 从烟曲霉（AfAmy1）提供真菌α-淀粉酶。 AfAmy1具有3.5的最佳pH并且在30-75摄氏度下可操作，允许酶在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AfAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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9.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION 有权从ASPERGILLUS FUMIGATUS和ISOAMYLASE使用ALPHA-AMYLASE进行鉴定的方法公开(公告)号：US20160032338A1
公开(公告)日：2016-02-04
申请号：US14649173
申请日：2013-12-05
Applicant: DANISCO US INC.
Inventor： Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang , Zhengong Zhang
CPC classification number: C12P19/14 , A21D8/042 , A23L7/107 , A23V2002/00 , C11D3/386 , C12C5/004 , C12C11/003 , C12N9/242 , C12N9/246 , C12P7/06 , C12P19/02 , C12Y302/01001 , C12Y302/01068 , D06L1/06 , Y02E50/17
Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmyl). AfAmyl has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DPI+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由烟曲霉（AfAmyl）提供。 AfAmyl具有3.5的最佳pH并且在30-75摄氏度下可操作，允许酶在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川崎曲霉α-淀粉酶催化的糖化产物相比，AfAmyl还催化淀粉底物对显着富集DP2和（DPI + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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10.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS TERREUS AND ISOAMYLASE FOR SACCHARIFICATION 审中-公开从ASPERGILLUS TERREUS和ISOAMYLASE使用ALPHA-AMYLASE进行分类的方法公开(公告)号：US20150376668A1
公开(公告)日：2015-12-31
申请号：US14649805
申请日：2013-12-06
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Martijn Scheffers , Marco Van Brussel , Casper Vroemen , Bo Zhang
CPC classification number: C12P19/14 , A21D8/042 , A23L2/382 , A23L7/107 , A23L29/35 , C11D3/38618 , C11D3/38681 , C12C5/004 , C12N9/242 , C12P7/14 , C12P19/02 , D06L1/14 , D06M16/003
Abstract: A fungal alpha-amylase is provided from Aspergillus terreus (AtAmy1). AtAmy1 has an optimal pH of 4.5 and is operable at 30 75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and an isoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AtAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the ligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 从Aspergillus terreus（AtAmy1）提供真菌α-淀粉酶。 AtAmy1具有4.5的最佳pH，并且在30℃下可操作，允许酶在糖化反应中与葡糖淀粉酶和异淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，AtAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有利于在同时糖化和发酵过程中由发酵生物体利用该链状糖组合物。
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