公开(公告)号：US09365871B2

公开(公告)日：2016-06-14

申请号：US14561085

申请日：2014-12-04

申请人： DANISCO US INC.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P19/14 ,  C12C7/04 ,  C12P7/14

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US08945889B2

公开(公告)日：2015-02-03

申请号：US13888303

申请日：2013-05-06

申请人： Danisco US Inc.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P7/36 ,  C12P7/02 ,  C12P19/14 ,  C12C7/04

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmyl）提供。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20160068879A1

公开(公告)日：2016-03-10

申请号：US14419906

申请日：2013-08-12

申请人： DANISCO US INC.

发明人： Su Yin Maria Chow ,  Thomas P. Graycar ,  Jacquelyn A. Huitink ,  Casper Vroemen ,  David L. Wong

IPC分类号： C12P19/20 ,  C12P7/10 ,  C12P19/02 ,  C12N9/34

CPC分类号： C12P19/20 ,  C12N9/2428 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01003 ,  Y02E50/16 ,  Y02P20/52

摘要： Embodiments of the present disclosure relate to Trichoderma reesei glucoamylase (TrGA) variants having improved properties (e.g., improved thermostability, improved specific activity, and/or resistant to oxidation-related activity loss). Also provided are compositions comprising variant glucoamylases. These compositions are useful in various starch process applications.

摘要翻译： 本公开的实施方案涉及具有改善的性质（例如改善的热稳定性，改善的比活性和/或耐氧化相关活性损失）的里氏木霉葡糖淀粉酶（TrGA）变体。 还提供了包含变体葡糖淀粉酶的组合物。 这些组合物可用于各种淀粉工艺应用。

公开(公告)号：US20150218606A1

公开(公告)日：2015-08-06

申请号：US14419902

申请日：2013-08-13

申请人： DANISCO US INC.

发明人： Marco van Brussel-Zwijnen ,  Jacquelyn A. Huitink ,  Martijn Silvan Scheffers ,  Paula Johanna Maria Teunissen ,  Casper Vroemen

IPC分类号： C12P19/02 ,  C12N9/30 ,  C12P19/16 ,  A21D8/04 ,  C11D3/386 ,  C12C5/00 ,  C12C11/00 ,  C12N9/44 ,  C12P7/14

CPC分类号： C12P19/02 ,  A21D8/042 ,  C11D3/386 ,  C11D3/38636 ,  C12C5/004 ,  C12C11/003 ,  C12N9/242 ,  C12N9/2457 ,  C12P7/06 ,  C12P7/14 ,  C12P19/14 ,  C12P19/16 ,  C12Y302/01001 ,  C12Y302/01041 ,  D06L1/14 ,  D06L4/40 ,  Y02E50/17

摘要： A fungal alpha amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75 C, allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 从曲霉菌（AcAmyl）提供真菌α淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，使得酶可在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为间歇方法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α淀粉酶或葡糖淀粉酶。 AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化，与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20150152442A1

公开(公告)日：2015-06-04

申请号：US14561085

申请日：2014-12-04

申请人： DANISCO US INC.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P7/14 ,  C12C7/04

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20170183691A1

公开(公告)日：2017-06-29

申请号：US15305036

申请日：2015-04-06

申请人： Danisco US Inc.

发明人： Kathleen A. Clarkson ,  Matthew T. Reboli ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen ,  Gopal K. Chotani ,  Jayarama K. Shetty

IPC分类号： C12P7/14

CPC分类号： C12P7/14 ,  C12P7/06 ,  Y02E50/17

摘要： A conversion process provides using different co-cultured cell lines to express different sets of enzymes catalyzing the same process. For example, in a Simultaneous Saccharification and Co-Fermentation (SSCF) process, a starch substrate is converted to alcohol by contacting the substrate with yeast and Aspergillus niger cells. Because A. niger expresses an endogenous glucoamylase and alpha-amylase, these enzymes do not need to be added during the SSCF process.

公开(公告)号：US09540669B2

公开(公告)日：2017-01-10

申请号：US14419906

申请日：2013-08-12

申请人： DANISCO US INC.

发明人： Su Yin Marina Chow ,  Thomas P. Graycar ,  Jacquelyn A. Huitink ,  Casper Vroemen ,  David L. Wong

IPC分类号： C12N9/34 ,  C12P19/20 ,  C12P19/14 ,  C12P19/02 ,  C12P7/10

CPC分类号： C12P19/20 ,  C12N9/2428 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01003 ,  Y02E50/16 ,  Y02P20/52

摘要： Embodiments of the present disclosure relate to Trichoderma reesei glucoamylase (TrGA) variants having improved properties (e.g., improved thermostability, improved specific activity, and/or resistant to oxidation-related activity loss). Also provided are compositions comprising variant glucoamylases. These compositions are useful in various starch process applications.

摘要翻译： 本公开的实施方案涉及具有改善的性质（例如改善的热稳定性，改善的比活性和/或耐氧化相关活性损失）的里氏木霉葡糖淀粉酶（TrGA）变体。 还提供了包含变体葡糖淀粉酶的组合物。 这些组合物可用于各种淀粉工艺应用。

公开(公告)号：US20130323798A1

公开(公告)日：2013-12-05

申请号：US13888303

申请日：2013-05-06

申请人： Danisco US Inc.

发明人： Jing Ge ,  Ling Hua ,  Martijn Silvan Scheffers ,  Zhongmei Tang ,  Marco Van Brussel-Zwijnen ,  Casper Vroemen ,  Bo Zhang ,  Kathleen A. Clarkson ,  Jacquelyn A. Huitink ,  Paula Johanna Maria Teunissen

IPC分类号： C12P19/14 ,  C12C7/04

CPC分类号： C12P7/14 ,  C12C7/04 ,  C12P19/14 ,  C12P2201/00 ,  Y02E50/17

摘要： A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

摘要翻译： 从曲霉菌（AcAmyl）提供真菌α-淀粉酶。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。