公开(公告)号：US20220195399A1

公开(公告)日：2022-06-23

申请号：US17528564

申请日：2021-11-17

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Natnael Behabtu ,  Zhenghong Zhang ,  Laurie A. Howe

IPC: C12N9/10 ,  C08B37/00 ,  C12P19/04

Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.

公开(公告)号：US20150240223A1

公开(公告)日：2015-08-27

申请号：US14433941

申请日：2013-09-17

Applicant: DANISCO US INC.

Inventor： Ling Hua ,  Zhongmei Tang ,  Casper Willem Vroemen ,  Bo Zhang ,  Zhenghong Zhang

IPC: C12N9/30 ,  C12P7/14 ,  A21D8/04 ,  C11D3/386 ,  C12C11/07 ,  C12P19/02 ,  C12P19/14

CPC classification number: C12N9/242 ,  A21D8/042 ,  A23L29/35 ,  C11D3/386 ,  C12C5/004 ,  C12C7/04 ,  C12C7/14 ,  C12C11/003 ,  C12C11/07 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01001 ,  D06L1/12 ,  D06L1/14 ,  D06M16/003 ,  Y02E50/16 ,  Y02E50/17

Abstract: A fungal alpha amylase is provided from Talaromyces emersonii (TeAmy1), along with variants of the same. TeAmy1 has an optimal pH of 3.5 and is operable over a temperature range of at least 30 75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha amylase or glucoamylase. TeAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 真菌α淀粉酶从艾美（Talaromyces emersonii）（TeAmy1）提供，以及相同的变体。 TeAmy1具有3.5的最佳pH，并且在至少3075℃的温度范围内是可操作的，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇方法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α淀粉酶或葡糖淀粉酶。 与由来自曲霉（Aspergillus kawachii）的α淀粉酶催化的糖化产物相比，TeAmy1还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US10294466B2

公开(公告)日：2019-05-21

申请号：US15553134

申请日：2016-02-25

Applicant: DANISCO US INC.

Inventor： Zheyong Yu ,  Zhenghong Zhang ,  Jing Ge ,  Zhen Qian ,  Guoqing Liu ,  Zhiyong Xie ,  Zhongmei Tang

IPC: C12N9/26 ,  C12N1/20 ,  C12N1/14 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18 ,  C12P19/22 ,  C12P7/10 ,  C12C5/00 ,  C12C12/02 ,  A23L33/18 ,  A23L2/52 ,  C12P19/02

Abstract: The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.

公开(公告)号：US11802275B2

公开(公告)日：2023-10-31

申请号：US17261937

申请日：2019-07-18

Applicant: DANISCO US INC

Inventor： Slavko Kralj ,  Zheyong Yu ,  Zhenghong Zhang

IPC: C12P19/04 ,  C12N9/10

CPC classification number: C12N9/1051 ,  C12P19/04 ,  C12Y204/01001 ,  C12Y204/01007

Abstract: Reactions are disclosed herein comprising water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and an alpha-1,4-glucan phosphorylase. Novel alpha-1,4-glucan phosphorylase enzymes are also disclosed. Additional disclosures herein regard sucrose phosphorylase enzymes and methods of use thereof to produce alpha-G1P.

公开(公告)号：US20190002937A1

公开(公告)日：2019-01-03

申请号：US16064552

申请日：2016-12-20

Applicant: Danisco US Inc.

Inventor： Bart C. Koops ,  Paula Johanna Maria Teunissen ,  Marco Van Brussel-Zwijnen ,  Martijn Scheffers ,  Kees-Jan Guijt ,  Zhengzheng Zou ,  Zhongmei Tang ,  Zhen Qian ,  Jing Ge ,  Zhenghong Zhang

IPC: C12P19/02 ,  C12P19/14 ,  C12N9/34 ,  C12N9/30

Abstract: Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.

公开(公告)号：US20160040202A1

公开(公告)日：2016-02-11

申请号：US14649170

申请日：2013-12-05

Applicant: DANISCO US INC.

Inventor： Ling Hua ,  Martijn Scheffers ,  Marco Van Brussel ,  Casper Vroemen ,  Bo Zhang ,  Zhenghong Zhang

IPC: C12P19/02 ,  A23L2/38 ,  C12C5/00 ,  C12P19/14 ,  C12P7/14

CPC classification number: C12P19/02 ,  A21D8/042 ,  A23L2/382 ,  C11D3/38636 ,  C12C5/004 ,  C12C7/04 ,  C12C7/16 ,  C12C11/003 ,  C12C12/04 ,  C12G3/02 ,  C12N9/242 ,  C12P7/14 ,  C12P19/14 ,  C12Y302/01001 ,  C13K1/06 ,  D06L1/06

Abstract: A fungal alpha-amylase is provided from Aspergillus fumigatus (AfAmy1). AfAmy1 has an optimal pH of 3.5 and is operable at 30-75 degrees C., allowing the enzyme to be used in combination with a glucoamylase and a pullulanase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase. AfAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an alpha-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.

Abstract translation: 从烟曲霉（AfAmy1）提供真菌α-淀粉酶。 AfAmy1具有3.5的最佳pH并且在30-75摄氏度下可操作，允许酶在糖化反应中与葡糖淀粉酶和支链淀粉酶组合使用。 这消除了作为分批方法进行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AfAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。

公开(公告)号：US20240384205A1

公开(公告)日：2024-11-21

申请号：US18687521

申请日：2022-09-01

Applicant: Danisco US Inc.

Inventor： Jonathan Lassila ,  Zhenghong Zhang ,  Kun Zhong

IPC: C11D3/386 ,  C12N9/22

Abstract: Disclosed herein are compositions and methods for preventing, reducing, or removing biofilms.

公开(公告)号：US11180740B2

公开(公告)日：2021-11-23

申请号：US16383820

申请日：2019-04-15

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Natnael Behabtu ,  Zhenghong Zhang ,  Laurie A Howe

IPC: C12N9/10 ,  C12P19/04 ,  C08B37/00

Abstract: Reaction compositions are disclosed herein comprising at least water, alpha-glucose-1-phosphate (alpha-G1P), an acceptor molecule, and a beta-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with beta-1,3 glycosidic linkages. Further disclosed are methods of isolating beta-1,3-glucan.

公开(公告)号：US10808269B2

公开(公告)日：2020-10-20

申请号：US16281225

申请日：2019-02-21

Applicant: DANISCO US INC

Inventor： Zheyong Yu ,  Slavko Kralj ,  Zhenghong Zhang ,  Laurie A. Howe

IPC: C12P19/08 ,  C12N9/10 ,  C12P19/04 ,  C12P19/18

Abstract: Reaction compositions are disclosed herein comprising at least water, beta-glucose-1-phosphate (beta-G1P), an acceptor molecule, and an alpha-1,3-glucan phosphorylase enzyme. These reactions can synthesize oligosaccharides and polysaccharides with alpha-1,3 glycosidic linkages. Further disclosed are alpha-1,3-glucan phosphorylase enzymes and methods of use thereof.

公开(公告)号：US20200277632A1

公开(公告)日：2020-09-03

申请号：US16645687

申请日：2018-08-30

Applicant: DANISCO US INC.

Inventor： Zhongmei Tang ,  Helong Hao ,  Zhiyong Xie ,  Zhenghong Zhang

IPC: C12P7/06 ,  C13K1/06 ,  C12N9/34

Abstract: Described are methods of saccharifying starch-containing materials using a glucoamylase, as well as the methods of producing fermentation products and the fermentation products produced by the method thereof.