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1.发明申请公开(公告)号：US20190177757A1
公开(公告)日：2019-06-13
申请号：US16281989
申请日：2019-02-21
Applicant: DANISCO US INC
Inventor： Jing Ge , Ling Hua , Sung Ho Lee , Jalsen Li , Jayarama K Shetty , Zhongmei Tang , Bo Zhang , Kun Zhong
CPC classification number: C12P19/14 , C12N9/2428 , C12P19/02 , C12Y302/01003 , Y02E50/17
Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
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公开(公告)号：US10294466B2
公开(公告)日：2019-05-21
申请号：US15553134
申请日：2016-02-25
Applicant: DANISCO US INC.
Inventor： Zheyong Yu , Zhenghong Zhang , Jing Ge , Zhen Qian , Guoqing Liu , Zhiyong Xie , Zhongmei Tang
IPC: C12N9/26 , C12N1/20 , C12N1/14 , C12P19/14 , C12P19/16 , C12P19/18 , C12P19/22 , C12P7/10 , C12C5/00 , C12C12/02 , A23L33/18 , A23L2/52 , C12P19/02
Abstract: The present disclosure relates to polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia or engineered polypeptides having alpha-glucosidase activity isolated, derived or derivable from Rasamsonia homologs. The present disclosure also pertains to polynucleotides encoding the polypeptides, nucleic acid constructs, vectors, host cells and mutant cells comprising the polynucleotides. The disclosure further pertains to compositions comprising such polypeptides, methods of producing the polypeptides and compositions, as well as methods for using such polypeptides and compositions for industrial applications.
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3.发明申请PROCESS FOR PRODUCING HIGH GLUCOSE COMPOSITIONS BY SIMULTANEOUS LIQUEFACTION AND SACCHARIFICATION OF STARCH SUBSTRATES 审中-公开生产高葡萄糖组合物的方法同时进行液相色谱和淀粉底物的分离公开(公告)号：US20160108448A1
公开(公告)日：2016-04-21
申请号：US14649168
申请日：2013-11-21
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Sung Ho Lee , Jayarama K. Shetty , Bruce A. Strohm , Zhongmei Tang , Donald E. Ward
CPC classification number: C12P19/16 , C12C5/004 , C12C12/00 , C12P19/02 , C12P19/14 , C12P19/20 , C13K1/06
Abstract: Fungal glucoamylases from Aspergillus fumigatus that are expressed in Trichoderma reesei host cells (AfGATR) are provided. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a simultaneous liquefaction and saccharification process in the presence of alpha amylase, such as Aspergillus kawachii alpha-amylase (AkAA). This greatly reduces the combined run time of liquefaction and saccharification reaction, where the pH and temperature must be readjusted for optimal use of the alpha-amylase or glucoamylase.
Abstract translation: 提供了在里氏木霉宿主细胞（AfGATR）中表达的来自烟曲霉的真菌葡糖淀粉酶。 包括AfGA1TR和AfGA2TR的AfGATR在高温和低pH下表现出高活性，因此在α淀粉酶如川曲霉α-淀粉酶（AkAA）的存在下，AfGATR可以有效地用于同时液化和糖化过程。 这大大降低了液化和糖化反应的组合运行时间，其中必须重新调整pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。
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4.发明授权Method of using alpha-amylase from Aspergillus clavatus for saccharification 有权使用来自曲霉菌的α-淀粉酶进行糖化的方法公开(公告)号：US08945889B2
公开(公告)日：2015-02-03
申请号：US13888303
申请日：2013-05-06
Applicant: Danisco US Inc.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmyl). AcAmyl has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmyl also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmyl）提供。 AcAmyl具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmyl还催化淀粉底物对显着富集DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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公开(公告)号：US20230340443A1
公开(公告)日：2023-10-26
申请号：US18151122
申请日：2023-01-06
Applicant: Danisco US Inc.
Inventor： Bart C. Koops , Paula Johanna Maria Teunissen , Marco Van Brussel-Zwijnen , Martijn Scheffers , Kees-Jan Guijt , Zhengzheng Zou , Zhongmei Tang , Zhen Qian , Jing Ge , Zhenghong Zhang
CPC classification number: C12N9/242 , C12P19/02 , C12P19/14 , C12Y302/01001 , C12Y302/01003 , C12N9/2428 , C12P7/06
Abstract: Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch.
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Patent Agency Ranking
公开(公告)号：US11525151B2
公开(公告)日：2022-12-13
申请号：US16977896
申请日：2019-03-06
Applicant: DANISCO US INC
Inventor： Jing Ge , Xiaogang Gu , Helong Hao , Karsten Matthias Kragh , Jinahua (Jalsen) Li , Wenting Li , Zhongmei Tang , Shukun Yu , Bo Zhong , Kun Zhong , Zhengzheng Zou
Abstract: Described are methods for saccharifying starch-containing materials using a glucoamylase, methods for producing fermentation products, and fermentation products produced by the method thereof as well as methods for increasing starch digestibility in a ruminant using at least one of the glucoamylases described herein.
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公开(公告)号：US10767207B2
公开(公告)日：2020-09-08
申请号：US16281989
申请日：2019-02-21
Applicant: DANISCO US INC
Inventor： Jing Ge , Ling Hua , Sung Ho Lee , Jalsen Li , Jayarama K Shetty , Zhongmei Tang , Bo Zhang , Kun Zhong
Abstract: Fungal glucoamylases from Aspergillus fumigatus—expressed in Trichoderma reesei host cells (AfGATR) are provided. Trichoderma reesei host cells express AfGATRs at higher, or at least comparable, levels to natively expressed AfGA Aspergillus fumigatus. AfGATRs, including AfGA1TR and AfGA2TR, exhibit high activity at elevated temperatures and at low pH, so AfGATRs can be used efficiently in a process of saccharification in the presence of alpha-amylase, such as Aspergillus kawachii alpha-amylase (AkAA). AfGATRs advantageously catalyze starch saccharification to an oligosaccharide composition significantly enriched in DP1 (i.e., glucose) compared to the products of saccharification catalyzed by Aspergillus niger glucoamylase (AnGA) or native AfGA expressed in Aspergillus fumigatus. AfGATRs such as AfGA1TR, AfGA2TR or a variant thereof can be used at a lower dosage than AnGA and natively expressed AfGAs to produce comparable levels of glucose.
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8.发明申请公开(公告)号：US20160160199A1
公开(公告)日：2016-06-09
申请号：US14907718
申请日：2014-09-19
Applicant: DANISCO US INC.
Inventor： Guoqing Liu , Ling Hua , Zhen Qian , Danfeng Song , Zhongmei Tang , Xie Zhiyong , Bo Zhang , Zhengzheng Zou , Wei Xu
Abstract: Disclosed are compositions and methods relating to alpha-amylase from Exiguobacterium. The compositions and methods are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and and for baking and brewing.
Abstract translation: 公开了与来自Exiguobacterium的α-淀粉酶相关的组合物和方法。 组合物和方法可用于例如淀粉液化和糖化，用于清洁衣物，餐具洗涤和其它用途中的淀粉污渍，用于织物加工（例如脱浆），动物饲料中用于改善消化率，以及用于烘烤和 酿造。
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9.发明申请METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION 审中-公开使用来自青蒿素的ALPHA-AMYLASE进行分离的方法公开(公告)号：US20150152442A1
公开(公告)日：2015-06-04
申请号：US14561085
申请日：2014-12-04
Applicant: DANISCO US INC.
Inventor： Jing Ge , Ling Hua , Martijn Silvan Scheffers , Zhongmei Tang , Marco Van Brussel-Zwijnen , Casper Vroemen , Bo Zhang , Kathleen A. Clarkson , Jacquelyn A. Huitink , Paula Johanna Maria Teunissen
CPC classification number: C12P7/14 , C12C7/04 , C12P19/14 , C12P2201/00 , Y02E50/17
Abstract: A fungal α-amylase is provided from Aspergillus clavatus (AcAmy1). AcAmy1 has an optimal pH of 4.5 and is operable at 30-75° C., allowing the enzyme to be used in combination with a glucoamylase in a saccharification reaction. This obviates the necessity of running a saccharification reaction as a batch process, where the pH and temperature must be readjusted for optimal use of the α-amylase or glucoamylase. AcAmy1 also catalyzes the saccharification of starch substrates to an oligosaccharide composition significantly enriched in DP2 and (DP1+DP2) compared to the products of saccharification catalyzed by an α-amylase from Aspergillus kawachii. This facilitates the utilization of the oligosaccharide composition by a fermenting organism in a simultaneous saccharification and fermentation process, for example.
Abstract translation: 真菌α-淀粉酶由曲霉菌（AcAmy1）提供。 AcAmy1具有4.5的最佳pH，并且在30-75℃下可操作，允许酶在糖化反应中与葡糖淀粉酶组合使用。 这消除了作为间歇法运行糖化反应的必要性，其中必须重新调节pH和温度以最佳地使用α-淀粉酶或葡糖淀粉酶。 与由川曲霉α-淀粉酶催化的糖化产物相比，AcAmy1还催化淀粉底物对显着富含DP2和（DP1 + DP2）的寡糖组合物的糖化。 这有助于例如在同时糖化和发酵过程中由发酵生物体利用寡糖组合物。
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公开(公告)号：US20230002749A1
公开(公告)日：2023-01-05
申请号：US16491078
申请日：2017-11-09
Applicant: Danisco US Inc.
Inventor： Jacob Flyvholm Cramer , Jing Ge , Marc Anton Bernhard Kolkman , Igor Nikolaev , Jayarama K. Shetty , Zhongmei Tang , Wilhelmus Antonius Van Der Kleij , Zhiyong Xie , Bo Zhang , Zhengzheng Zou
Abstract: Described are polypeptides having glucoamylase activity, compositions comprising such polypeptides, and methods of using such polypeptides and compositions.
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