公开(公告)号：US20190194692A1

公开(公告)日：2019-06-27

申请号：US15541665

申请日：2016-01-06

Applicant: DSM IP ASSETS B.V. ,  RIJKSUNIVERSITEIT GRONINGEN

Inventor： Bernard MEIJRINK ,  René VERWAAL ,  Bianca Elisabeth Maria GIELESEN ,  Johannes Andries ROUBOS ,  Carsten POHL ,  Arnold Jacob Mattieu DRIESSEN ,  Roelof Ary Lans BOVENBERG ,  Yvonne Irene NYGÅRD

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

CPC classification number: C12N15/902 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2800/80

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.

公开(公告)号：US20170191089A1

公开(公告)日：2017-07-06

申请号：US15314492

申请日：2015-05-28

Applicant: DSM IP Assets B.V.

Inventor： Zheng ZHAO ,  Bernard MEIJRINK ,  Robertus Antonius Mijindert VAN DER HOEVEN ,  Liang WU ,  Johannes Andries ROUBOS

IPC: C12P7/44 ,  C12N9/10 ,  C12N15/52 ,  C07K14/395 ,  C12P7/62 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/44 ,  C07K14/395 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/03001 ,  C12Y401/01006 ,  C12Y401/01031 ,  C12Y401/01032 ,  C12Y604/01001

Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.

公开(公告)号：US20200172934A1

公开(公告)日：2020-06-04

申请号：US16780063

申请日：2020-02-03

Applicant: DSM IP ASSETS B.V.

Inventor： René VERWAAL ,  Bernard MEIJRINK ,  Nathalie WIESSENHAAN ,  Brenda VONK ,  Johannes Andries ROUBOS

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22 ,  C12N15/81 ,  C12N15/10 ,  C12P23/00

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.

公开(公告)号：US20180010151A1

公开(公告)日：2018-01-11

申请号：US15541431

申请日：2016-01-06

Applicant: DSM IP ASSETS B.V.

Inventor： René VERWAAL ,  Bernard MEIJRINK ,  Nathalie WIESSENHAAN ,  Brenda VONK ,  Johannes Andries ROUBOS

IPC: C12N15/90 ,  C12N15/113 ,  C12N9/22 ,  C12P23/00

CPC classification number: C12N15/905 ,  C12N9/22 ,  C12N15/102 ,  C12N15/113 ,  C12N15/81 ,  C12N15/902 ,  C12N2310/20 ,  C12P23/00

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.

公开(公告)号：US20200165637A1

公开(公告)日：2020-05-28

申请号：US16780389

申请日：2020-02-03

Applicant: DSM IP ASSETS B.V.

Inventor： Bernard MEIJRINK ,  René VERWAAL ,  Bianca Elisabeth Maria GIELESEN ,  Johannes Andries ROUBOS

IPC: C12N15/90 ,  C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.

公开(公告)号：US20170191091A1

公开(公告)日：2017-07-06

申请号：US15314494

申请日：2015-05-28

Applicant: DSM IP Assets B.V.

Inventor： Zheng ZHAO ,  Bernard MEIJRINK ,  Robertus Antonius Mijndert VAN DER HOEVEN ,  Liang WU ,  Johannes Andries ROUBOS ,  Marina VAN GELDER

IPC: C12P7/62 ,  C12N15/52 ,  C12P7/46

CPC classification number: C12P7/62 ,  C12N15/52 ,  C12P7/46

Abstract: The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid, 4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.