公开(公告)号：US20160319245A1

公开(公告)日：2016-11-03

申请号：US15103237

申请日：2014-12-12

Applicant: DSM IP ASSETS B.V.

Inventor： René Marcel DE JONG ,  Zheng ZHAO ,  Ben DEN DULK ,  Remko Tsjibbe WINTER

IPC: C12N9/02 ,  C12P7/46

CPC classification number: C12N9/001 ,  C12P7/46 ,  C12Y103/01006

Abstract: The present invention relates to a variant polypeptide having fumarate reductase activity, which has modified NADP(H)-dependent and/or NAD(H)-dependent activity as compared with a reference polypeptide having fumarate reductase activity. Such a variant may be overexpressed in a host cell in order to improve production of a dicarboxylic acid.

Abstract translation: 本发明涉及具有富马酸酯还原酶活性的变体多肽，其与具有富马酸盐还原酶活性的参照多肽相比具有修饰的NADP（H） - 依赖和/或NAD（H） - 依赖性活性。 这种变体可以在宿主细胞中过表达，以改善二羧酸的产生。

公开(公告)号：US20180245106A1

公开(公告)日：2018-08-30

申请号：US15314489

申请日：2015-05-28

Applicant: DSM IP Assets B.V.

Inventor： Zheng ZHAO

IPC: C12P7/44 ,  C12P7/62

CPC classification number: C12P7/44 ,  C12P7/46 ,  C12P7/62

Abstract: The present invention relates to a method for the production of itaconic acid or an ester of itaconic acid, which method comprises fermenting a recombinant cell capable of producing itaconic acid or an ester of itaconic acid in a suitable fermentation medium under anaerobic conditions, wherein the itaconic acid or ester of itaconic acid is produced.

公开(公告)号：US20170191091A1

公开(公告)日：2017-07-06

申请号：US15314494

申请日：2015-05-28

Applicant: DSM IP Assets B.V.

Inventor： Zheng ZHAO ,  Bernard MEIJRINK ,  Robertus Antonius Mijndert VAN DER HOEVEN ,  Liang WU ,  Johannes Andries ROUBOS ,  Marina VAN GELDER

IPC: C12P7/62 ,  C12N15/52 ,  C12P7/46

CPC classification number: C12P7/62 ,  C12N15/52 ,  C12P7/46

Abstract: The present invention relates to a recombinant cell which is capable of producing one or more of itaconic acid, 4-methyl itaconate or 1-methyl itaconate, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. A recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —one or more nucleic acids encoding polypeptides which separately or together catalyze a reaction towards acetyl CoA, wherein the said recombinant microorganism has been modified in its genome such that it results in a deficiency in the production of a trans-aconitate methyltransferase. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate or 1-methyl itaconate.

公开(公告)号：US20200181657A1

公开(公告)日：2020-06-11

申请号：US16063810

申请日：2016-12-22

Applicant: DSM IP ASSETS B.V.

Inventor： Alrik Pieter LOS ,  Zheng ZHAO ,  René VERWAAL ,  Jacobus Thomas PRONK ,  Rolf POLDERMANS

IPC: C12P7/46 ,  C12N9/02 ,  C12N9/04 ,  C12N15/81

Abstract: The present invention relates to a host cell which is capable of producing a dicarboxylic acid and which comprises at least one genetic modification in its genome resulting in the deficiency of at least one enzymatic step catalysing the oxidation of a cofactor. The invention also relates to a process for producing a dicarboxylic acid, which method comprises fermenting such a host cell in a suitable fermentation medium and producing the dicarboxylic acid.

公开(公告)号：US20170191089A1

公开(公告)日：2017-07-06

申请号：US15314492

申请日：2015-05-28

Applicant: DSM IP Assets B.V.

Inventor： Zheng ZHAO ,  Bernard MEIJRINK ,  Robertus Antonius Mijindert VAN DER HOEVEN ,  Liang WU ,  Johannes Andries ROUBOS

IPC: C12P7/44 ,  C12N9/10 ,  C12N15/52 ,  C07K14/395 ,  C12P7/62 ,  C12N9/88 ,  C12N9/00

CPC classification number: C12P7/44 ,  C07K14/395 ,  C12N9/0008 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12P7/62 ,  C12Y203/03001 ,  C12Y401/01006 ,  C12Y401/01031 ,  C12Y401/01032 ,  C12Y604/01001

Abstract: The present invention relates to a recombinant yeast cell which is capable of producing one or more of 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate. The invention also relates to a recombinant yeast cell which is capable of producing itaconic acid and which overexpresses: —a nucleic acid encoding a polypeptide having cis-aconitate decarboxylase activity; and —a nucleic acid encoding a polypeptide which catalyzes a reaction towards acetyl CoA. These recombinant yeast cells may be used in processes for the production of itaconic acid, 4-methyl itaconate, 1-methyl itaconate or 1,4-dimethyl itaconate.