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公开(公告)号：US06441894B1
公开(公告)日：2002-08-27
申请号：US09430418
申请日：1999-10-29
Applicant: Bala S. Manian , David M. Heffelfinger , Edward M. Goldberg
Inventor： Bala S. Manian , David M. Heffelfinger , Edward M. Goldberg
IPC: G01J100
CPC classification number: G02B21/34 , G01N21/6452 , G02B21/241
Abstract: A method and apparatus for autofocus on a target layer contained within a microplate well is provided. The instrument is capable of optically sensing a reference point on the underside of a microplate. This reference point is then used to focus light onto a target layer within the microplate well, the target layer having a location that is in defined relation to the reference point. The reference point is either a surface of the bottom of the microplate well or is an optically detectable mark on the underside of the microplate. In an alternate embodiment, a light position sensitive detector is used to enable deterministic autofocus for a plurality of wells on a microplate.
Abstract translation: 提供了一种用于在微孔板中包含的靶层上的自动聚焦的方法和装置。 该仪器能够光学感测微孔板底面上的参考点。 然后将该参考点用于将光聚焦到微孔板内的目标层上，目标层具有与参考点限定的位置。 参考点是微孔板底部的表面，或者是微孔板下面的光学可检测标记。 在替代实施例中，使用光位置敏感检测器来使得微孔板上的多个孔的确定性自动聚焦。
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公开(公告)号：US5591981A
公开(公告)日：1997-01-07
申请号：US405468
申请日：1995-03-16
Applicant: David M. Heffelfinger , Franklin R. Witney
Inventor： David M. Heffelfinger , Franklin R. Witney
CPC classification number: G01N21/6456 , G01N21/64 , G01N2021/6419 , G01N2021/6421
Abstract: A method and apparatus to detect fluorescence from a sample is provided. The optical system allows the user to continuously tune the wavelengths and bandwidths of the excitation source and the emission detection system. A peaking function automatically peaks the detected fluorescent signal by fine tuning the excitation and emission detection systems. A look-up table allows the fluorescent signal for a specific wavelength to be corrected for the wavelength dependence of the optical train. In one embodiment of the system the sample is simultaneously irradiated in more than one wavelength band, each wavelength band being independently tunable.
Abstract translation: 提供了一种从样品中检测荧光的方法和装置。 光学系统允许用户连续调节激发源和发射检测系统的波长和带宽。 峰值功能通过微调激发和发射检测系统自动使检测到的荧光信号达到峰值。 查找表允许针对光学列车的波长依赖性校正特定波长的荧光信号。 在系统的一个实施例中，样本同时在多于一个波长带中照射，每个波长带是独立可调谐的。
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公开(公告)号：US5266803A
公开(公告)日：1993-11-30
申请号：US845107
申请日：1991-03-03
Applicant: David M. Heffelfinger
Inventor： David M. Heffelfinger
CPC classification number: H04N1/0281 , G02B26/103 , G02B6/32 , G02B6/403 , G02B6/3861
Abstract: A novel fiber optic cable having a bifurcated bundle of optical fibers comprised of emitter fiber(s) and collecting fiber(s), a means for focusing light into the proximal end of the emitter fiber(s), a means for focusing light out of the distal end of the emitter fiber(s), and a means for collecting light in the distal end of the collecting fiber(s) is used to increase the sensitivity and specificity of storage phosphor imaging plate scanning. The novel fiber optic cable is used in a storage phosphor imaging plate scanner to transmit light from a light source to the phosphors on the storage phosphor imaging plate and to collect any phosphorescence induced by the light. Use of the bifurcated fiber bundle with the means for focusing light into the emitter fiber(s), focus light out of the emitter fiber(s), and collect light in the collecting fiber(s) provides a method to scan storage phosphor imaging plates with high sensitivity and specificity.
Abstract translation: 一种新颖的光纤电缆，其具有由发射光纤和收集光纤组成的光纤的分叉束，用于将光聚焦到发射光纤的近端的装置，用于将光聚焦到 发射光纤的远端和用于收集聚光纤的远端的光的装置用于增加存储荧光体成像板扫描的灵敏度和特异性。 新型光纤电缆用于存储磷光体成像板扫描器中，以将来自光源的光透射到存储荧光体成像板上的荧光体，并收集由光诱导的任何磷光。 将分叉的光纤束与用于将光聚焦到发射光纤中的装置一起使用，将光聚焦在发射光纤之外，并收集聚光中的光提供了一种扫描存储荧光体成像板的方法 具有高灵敏度和特异性。
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公开(公告)号：US06853454B1
公开(公告)日：2005-02-08
申请号：US10758576
申请日：2004-01-15
Applicant: David M. Heffelfinger
Inventor： David M. Heffelfinger
CPC classification number: G01N21/6456
Abstract: A system for optical detection of kinetic samples. The system includes a dual set of detectors linked to a single processor. The time of signal integration is different for each detector, allowing one detector to have a higher sensitivity by integrating over a longer time period while the second detector using shorter integration periods is able to measure kinetic events.
Abstract translation: 一种用于光学检测动力学样品的系统。 该系统包括连接到单个处理器的双组检测器。 信号整合时间对于每个检测器是不同的，允许一个检测器通过在更长的时间段内积分而具有更高的灵敏度，而使用较短积分期的第二检测器能够测量动力学事件。
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公开(公告)号：US06750457B2
公开(公告)日：2004-06-15
申请号：US09942472
申请日：2001-08-29
Applicant: David M. Heffelfinger , Aram P. Schiffman , Bala S. Manian
Inventor： David M. Heffelfinger , Aram P. Schiffman , Bala S. Manian
IPC: G01N2100
CPC classification number: G01N21/6452 , G01J3/10 , G01J3/14 , G01J3/36 , G01J3/4406 , G01N27/44721 , G01N2021/6463
Abstract: An apparatus and method in which illumination light and collected emitted light share a pathway and subsequently are physically separated. The optical configuration is designed such that at the point of separation, the illumination light is at has a smaller cross sectional area than the collected light. Collected light is directed away from the pathway of the illumination light and to detection optics. This configuration is adaptable to illumination and light collection across a broad wavelength spectrum. This configuration is adaptable to scanning in a limited depth of field to allow high throughput optical analysis of samples.
Abstract translation: 照明光和收集的发射光共享通路并随后物理分离的装置和方法。 光学构造被设计成使得在分离点处，照明光具有比收集的光更小的横截面面积。 收集的光被引导离开照明光的路径和检测光学器件。 该配置适用于宽波长光谱下的照明和光采集。 该配置适用于在有限的景深中的扫描，以允许样品的高通量光学分析。
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Patent Agency Ranking
公开(公告)号：US6130745A
公开(公告)日：2000-10-10
申请号：US245782
申请日：1999-02-05
Applicant: Bala S. Manian , David M. Heffelfinger , Edward M. Goldberg
Inventor： Bala S. Manian , David M. Heffelfinger , Edward M. Goldberg
IPC: G01B11/00 , G01J1/00 , G01N21/03 , G01N21/55 , G01N21/64 , G01N33/483 , G02B7/28 , G02B21/24 , G02B21/34
CPC classification number: G02B21/34 , G01N21/6452 , G02B21/241
Abstract: A method and apparatus for autofocus on a target layer contained within a microplate well is provided. The instrument is capable of optically sensing a reference point on the underside of a microplate. This reference point is then used to focus light onto a target layer within the microplate well, the target layer having a location that is in defined relation to the reference point. The reference point is either a surface of the bottom of the microplate well or is an optically detectable mark on the underside of the microplate. In an alternate embodiment, a light position sensitive detector is used to enable deterministic autofocus for a plurality of wells on a microplate.
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公开(公告)号：US5784152A
公开(公告)日：1998-07-21
申请号：US729111
申请日：1996-10-11
Applicant: David M. Heffelfinger , Franklin R. Witney , Chris Cunanan
Inventor： David M. Heffelfinger , Franklin R. Witney , Chris Cunanan
CPC classification number: G01N21/64 , G01N21/253 , G01N21/6456 , G01N2021/6419 , G01N2021/6421
Abstract: A method and apparatus of analyzing samples contained in a microplate is provided. The instrument is capable of measuring fluorescence, luminescence, and/or absorption within multiple locations within a sample well. The instrument is tunable over the excitation and/or detection wavelengths. Neutral density filters are used to extend the sensitivity range of the absorption measuring aspect of the instrument. Due to the wavelength tuning capabilities of the instrument, the spectral dependence of the measured fluorescence, luminescence, and absorption of the materials in question can be analyzed. The combination of a data processor and a look-up table improve the ease of operation of the instrument. Several different formats are available for the output data including creation of a bit map of the sample.
Abstract translation: 提供了分析微孔板中包含的样品的方法和装置。 该仪器能够测量样品井内多个位置的荧光，发光和/或吸收。 仪器可以在激发和/或检测波长上调谐。 中性密度滤波器用于扩展仪器吸收测量方面的灵敏度范围。 由于仪器的波长调谐能力，可以分析所测材料的测量荧光，发光和吸收的光谱依赖性。 数据处理器和查找表的组合可以提高仪器的操作性。 输出数据可以使用几种不同的格式，包括创建样本的位图。
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公开(公告)号：US20080116814A1
公开(公告)日：2008-05-22
申请号：US11906573
申请日：2007-10-02
Applicant: David M. Heffelfinger , David A. Pringle , David B. McDonald , Ashot Nalbandyan
Inventor： David M. Heffelfinger , David A. Pringle , David B. McDonald , Ashot Nalbandyan
IPC: G03B15/02
CPC classification number: G03B15/05 , G03B2215/05
Abstract: A vehicle testing lamp system comprising an onboard controller unit, an off-board controller unit, and a lamp fixture. The onboard controller unit comprises a solid state relay, the solid state relay being switchable between an idle output power setting and a high output power setting; a timing circuit; a transfer circuit; a current sensor; a current regulator; and batteries or other auxiliary power supply. The off-board controller unit comprises a power supply electrically connected to a voltage booster circuit. The lamp fixture comprises an ignitor; a shock mount; and a lamp that is electrically connected to the igniter. The off-board controller supplies power to the lamp at its idle level. The onboard transfer circuit permits the off-board power supply to be disconnected while the onboard controller unit maintains the lamp at idle, and the onboard timing circuit limits the time that the lamp remains at high output. Also disclosed is a method for using a vehicle testing lamp system.
Abstract translation: 一种车辆测试灯系统，包括车载控制器单元，车外控制器单元和灯具。 车载控制器单元包括固态继电器，固态继电器可在空转输出功率设定和高输出功率设定之间切换; 定时电路; 传输电路; 电流传感器; 现行监管机构 电池或其他辅助电源。 车外控制器单元包括电连接到升压电路的电源。 灯具包括点火器; 一个防震架; 以及电连接到点火器的灯。 车外控制器在其空闲水平下为灯供电。 车载传输电路允许车载电源断开，而车载控制器单元将灯保持在空闲状态，并且车载定时电路限制灯保持在高输出的时间。 还公开了一种使用车辆测试灯系统的方法。
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公开(公告)号：US5951838A
公开(公告)日：1999-09-14
申请号：US814126
申请日：1997-03-10
Applicant: David M. Heffelfinger , Craig Van Horn
Inventor： David M. Heffelfinger , Craig Van Horn
IPC: G01N27/447 , G01N27/26 , G01N21/25 , G01J7/58
CPC classification number: G01N27/44721
Abstract: A method and apparatus for achieving uniform illumination in an electrophoresis apparatus is provided. Uniform illumination allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. Uniform illumination is achieved by scanning the light source, preferably in a trans-illumination mode, across the sample gel in a direction perpendicular to the axis of the source. The light source is comprised of one or more light bulbs placed in a light tray. Variations in light intensity near the source end portions may be minimized using a variety of techniques including extended light bulbs, filters, reflectors, diffusers, or supplemental sources.
Abstract translation: 提供了一种用于在电泳装置中实现均匀照明的方法和装置。 均匀的照明可以进行电泳凝胶的定量测量，从而增加从电泳分析可以获得的信息。 通过在垂直于源的轴线的方向上扫描样品凝胶上的光源，优选以跨照明模式扫描光源来实现均匀照明。 光源由放置在光盘中的一个或多个灯泡组成。 使用包括扩展的灯泡，滤光器，反射器，漫射器或补充源在内的各种技术可以最小化源端部附近的光强度的变化。
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10.发明授权
公开(公告)号：US5799773A
公开(公告)日：1998-09-01
申请号：US814125
申请日：1997-03-10
Applicant: David M. Heffelfinger , Craig Van Horn
Inventor： David M. Heffelfinger , Craig Van Horn
IPC: G01N27/447 , G01N27/26
CPC classification number: G01N27/44721
Abstract: A method and apparatus for correcting an image of an electrophoresis gel for lens and detector non-uniformities is provided. The removal of such non-uniformities, in conjunction with a uniform illumination source, allows quantitative measurements of an electrophoresis gel to be made, thus increasing the information which can be obtained from an electrophoretic analysis. Lens and detector non-uniformities are removed by first determining the magnitude of the non-uniformities using a calibration standard. The calibration standard has a uniform emittance, preferably in the same wavelength band as the labeled regions of the electrophoresis gel. By placing the calibration standard in close proximity to the entrance aperture of the lens, the calibration process is relatively insensitive to illumination source non-uniformities. Thus an image of the calibration standard taken with the same lens settings as the unknown provides detailed information on the lens and detector non-uniformities. This information in conjunction with a darkfield image is used to correct the sample image.
Abstract translation: 提供了用于校正透镜和检测器不均匀性的电泳凝胶的图像的方法和装置。 结合均匀的照明源去除这种不均匀性可以进行电泳凝胶的定量测量，从而增加可从电泳分析中获得的信息。 通过首先使用校准标准确定非均匀性的大小来去除透镜和检测器不均匀性。 校准标准具有均匀的发射率，优选地与电泳凝胶的标记区域相同的波长带。 通过将校准标准放置在靠近透镜入口孔的位置，校准过程对照明源不均匀性相对不敏感。 因此，以与未知的相同的镜头设置拍摄的校准标准的图像提供关于镜头和检测器不均匀性的详细信息。 该信息与暗场图像一起用于校正样本图像。
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