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    Lyase enzymes, nucleic acids encoding them and methods for making and using them
    1.
    发明授权
    Lyase enzymes, nucleic acids encoding them and methods for making and using them 有权
    裂解酶，编码它们的核酸和制备和使用它们的方法

    公开(公告)号：US08735107B2

    公开(公告)日：2014-05-27

    申请号：US13287567

    申请日：2011-11-02

    申请人： David P. Weiner ,  Alexander Varvak ,  Toby Richardson ,  Mircea Podar ,  Ellen Burke ,  Shaun Healey

    发明人： David P. Weiner ,  Alexander Varvak ,  Toby Richardson ,  Mircea Podar ,  Ellen Burke ,  Shaun Healey

    IPC分类号： C12P13/24 ,  C12P13/22

    CPC分类号： C12N9/88 ,  C12P7/42 ,  C12P13/222 ,  C12P13/225 ,  C12P13/24 ,  C12Y403/01005 ,  C12Y403/01023 ,  D06M16/003

    摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X═NO2, Cl, Br, NH2, OH, H, alkyl at one or several o, m, and p positions R═H or alkyl.

    摘要翻译： 本发明提供了具有裂解酶活性的多肽，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 一方面，本发明涉及具有氨裂解酶活性的多肽，例如苯丙氨酸氨裂解酶，酪氨酸氨裂解酶和/或组氨酸氨裂解酶活性，包括耐热和耐热活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸 和多肽。 本发明的多肽可用于多种药物，农业和工业环境。 在一个或几个o，m和p位置R = H或烷基时，X = NO 2，Cl，Br，NH 2，OH，H。

    Variant CBH I polypeptides
    5.
    发明授权
    Variant CBH I polypeptides 有权
    变体CBH I多肽

    公开(公告)号：US09234216B2

    公开(公告)日：2016-01-12

    申请号：US13824315

    申请日：2011-10-06

    申请人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    发明人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    IPC分类号： C12P7/14 ,  C12N9/42 ,  C12P19/02 ,  C12P19/14

    CPC分类号： C12P7/14 ,  C12N9/2437 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

    摘要： In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.

    摘要翻译： 在替代实施方案中，本发明提供具有木质纤维素分解（木质纤维素）活性的多肽，例如木质素分解和纤维素分解活性，包括例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶（cbhl）（例如，外切纤维二糖水解酶 例如具有可以逐步释放纤维二糖单元和-1,4葡萄糖 - 葡萄糖二糖的“外显子”活性，β-葡糖苷酶，木聚糖酶，甘露聚糖，木糖苷酶（例如，（β-谷氨酸） 和/或阿拉伯呋喃糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法在一个实施方案中，本发明提供了本发明的多肽的热稳定性和耐热性形式，使用本发明的多肽和核酸 在各种制药，农业和工业环境中;例如，作为生物质生物转化的酶，例如木质纤维素残基，i 在一方面，这些糖用作生产乙醇和燃料的化学原料，例如生物燃料，例如合成液体或气体燃料，包括乙醇，甲醇等。

    VARIANT CBH I POLYPEPTIDES
    10.
    发明申请
    VARIANT CBH I POLYPEPTIDES 有权
    变种CBH多糖

    公开(公告)号：US20130203128A1

    公开(公告)日：2013-08-08

    申请号：US13824315

    申请日：2011-10-06

    申请人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    发明人： Justin T. Stege ,  Alexander Varvak ,  John Poland ,  Chris S. Lyon ,  Shaun Healey ,  Peter Luginbuhl

    IPC分类号： C12N9/42 ,  C12P19/02

    CPC分类号： C12P7/14 ,  C12N9/2437 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

    摘要： In alternative embodiments, the invention provides polypeptides having a lignocellulolytic (lignocellulosic) activity, e.g., a ligninolytic and cellulolytic activity, including, e.g., a glycosyl hydrolase, a cellulase, an endoglucanase, a cellobiohydrolase (cbhl) (e.g., an exo-cellobiohydrolase, e.g., having an “exo” activity that can processively release cellobiose units β-1,4 glucose-glucose disaccharide), a beta-glucosidase, a xylanase, a mannanse, a xylosidase (e.g., a (β-xylosidase) and/or an arabinofuranosidase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one embodiment, the invention provides thermostable and thermotolerant forms of polypeptides of the invention. The polypeptides and nucleic acids of the invention are used in a variety of pharmaceutical, agricultural and industrial contexts; for example, as enzymes for the bioconversion of a biomass, e.g., lignocellulosic residues, into fermentable sugars, where in one aspect these sugars are used as a chemical feedstock for the production of ethanol and fuels, e.g., biofuels, e.g., synthetic liquid or gas fuels, including ethanol, methanol and the like.

    摘要翻译： 在替代实施方案中，本发明提供具有木质纤维素分解（木质纤维素）活性的多肽，例如木质素分解和纤维素分解活性，包括例如糖基水解酶，纤维素酶，内切葡聚糖酶，纤维二糖水解酶（cbhl）（例如，外切纤维二糖水解酶 例如，具有可以逐步释放纤维二糖单位β-1,4葡萄糖 - 葡萄糖二糖的“外部”活性），β-葡糖苷酶，木聚糖酶，甘露聚糖，木糖苷酶（例如（β-木糖苷酶）和/ 或阿拉伯呋喃糖苷酶活性，编码这些多肽的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法在一个实施方案中，本发明提供了本发明的多肽的耐热和耐热形式。本发明的多肽和核酸用于 各种制药，农业和工业环境;例如，作为生物质生物转化的酶，例如木质纤维素残基，转化为Fe 可再生糖，其中一方面，这些糖被用作用于生产乙醇和燃料的化学原料，例如生物燃料，例如合成液体或气体燃料，包括乙醇，甲醇等。