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公开(公告)号：US20240166318A1
公开(公告)日：2024-05-23
申请号：US17992641
申请日：2022-11-22
Applicant: David Root
Inventor： David Root
CPC classification number: B63C9/22 , B63C9/1255 , F41B11/62 , F41B11/80
Abstract: A projectile launcher assembly includes a housing mounted to the deck of a maritime vessel. The housing has a plurality of launching wells each extending into the housing. A plurality of projectiles is positioned within a respective one of the launching wells. A launching unit is positioned in the housing to launch each of the projectiles outwardly from the respective launching wells thereby facilitating the plurality of projectiles to be dispersed into a body of water in which the maritime vessel is traveling. A plurality of buoyancy vests is each positioned within a respective one of the projectiles. Each of the buoyancy vests is inflatable when the projectiles are launched from the housing to facilitate the individual that has fallen overboard to wear one of the buoyancy vests to inhibit the individual from drowning.
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2.发明申请公开(公告)号：US20080166270A1
公开(公告)日：2008-07-10
申请号：US12018600
申请日：2008-01-23
Applicant: Jean I. Montagu , Roger Dowd , David Root
Inventor： Jean I. Montagu , Roger Dowd , David Root
IPC: B01J19/00
CPC classification number: G01N33/544 , G01N33/54366
Abstract: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (14), silica-based materials and other materials are used to unite layers of the composite. In multiwell plates the oxide promotes joining of a bottom plate (95, 95′) and upper, well-defining structure (94) of dissimilar material. The oxides (14) also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
Abstract translation: 用于生物材料的固定装置包括承载具有生物固定性质的聚合物的基底层（20,20'）的刚性支撑体（12），例如。 用于氨基和核酸。 具有厚度小于约5微米，优选约0.1至0.5微米的基本上固体的超薄基底层（20'）和厚度小于约5微米的微孔超薄基底层（20'） ，优选小于3微米，2或1微米，其可以通过分离护羊M分段。基底层在显微镜载玻片（302），圆盘（122），生物盒，底部 多孔板，以及管内的涂层。 显示荧光或发光强度和几何校准点（420）。 通过强度校准点（420）增强读数，以使得在不均匀照射条件下的读数正常化，如通过暗场，侧面照明模式读取。 参考点被显示为同时印刷生物斑点阵列或用相同的设备打印。 形成装置层的方法包括从涂料组合物的浴中进行控制拉伸和干燥，以及C-D形基材的纺丝。 后处理通过电晕处理和辐射显示。 使用粘附金属氧化物（14），二氧化硅基材料和其他材料来组合复合材料层。 在多孔板中，氧化物促进底板（95,95'）和不同材料的上部，良好限定结构（94）的接合。 氧化物（14）还提供有益的不透明性，以防止光进入玻璃支撑体，以将电位施加到基底等。
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公开(公告)号：US5516564A
公开(公告)日：1996-05-14
申请号：US54442
申请日：1993-04-28
Applicant: David Root , Lewis Lawton
Inventor： David Root , Lewis Lawton
IPC: A61L2/08 , B01L3/02 , C08L25/06 , C08L27/12 , C08L101/00 , B29D22/00 , B32B27/00 , B65B1/04 , B65D97/18
CPC classification number: C08L25/06 , A61L2/08 , B01L3/0275 , C08L101/00 , C08L27/12 , Y10T428/1352 , Y10T428/139 , Y10T428/1397 , Y10T428/3154
Abstract: The present invention features hydrophobic articles of manufacture having a composition comprising a plastic and a fluoropolymer. The plastic-fluoropolymer composition is resistant to irradiation allowing the article to maintain hydrophobic properties through standard irradiation processes.
Abstract translation: 本发明的特征在于具有包含塑料和含氟聚合物的组合物的疏水制品。 塑料含氟聚合物组合物耐受照射，允许制品通过标准照射方法保持疏水性。
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4.发明授权公开(公告)号：US07384742B2
公开(公告)日：2008-06-10
申请号：US10524614
申请日：2003-08-18
Applicant: Jean I. Montagu , Roger Dowd , David Root
Inventor： Jean I. Montagu , Roger Dowd , David Root
IPC: C12Q1/68
CPC classification number: G01N33/544 , G01N33/54366
Abstract: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (14), silica-based materials and other materials are used to unite layers of the composite. In multi-well plates the oxide promotes joining of a bottom plate (95, 95′) and upper, well-defining structure (94) of dissimilar material. The oxides (14) also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
Abstract translation: 用于生物材料的固定装置包括承载具有生物固定性质的聚合物的基底层（20,20'）的刚性支撑体（12），例如。 用于氨基和核酸。 具有厚度小于约5微米，优选约0.1至0.5微米的基本上固体的超薄基底层（20'）和厚度小于约5微米的微孔超薄基底层（20'） ，优选小于3微米，2或1微米，其可以通过分离护羊M分段。基底层在显微镜载玻片（302），圆盘（122），生物盒，底部 多孔板，以及管内的涂层。 显示荧光或发光强度和几何校准点（420）。 通过强度校准点（420）增强读数，以使得在不均匀照射条件下的读数正常化，如通过暗场，侧面照明模式读取。 参考点被显示为同时印刷生物斑点阵列或使用相同的设备。形成设备层的方法包括从涂料组合物浴中进行控制拉伸和干燥，以及C-D形基材的纺丝。 后处理通过电晕处理和辐射显示。 使用粘附金属氧化物（14），二氧化硅基材料和其他材料来组合复合材料层。 在多孔板中，氧化物促进底板（95,95'）和不同材料的上部，良好限定结构（94）的接合。 氧化物（14）还提供有益的不透明性，以防止光进入玻璃支撑体，以将电位施加到基底等。
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5.发明申请公开(公告)号：US20060286550A1
公开(公告)日：2006-12-21
申请号：US11431997
申请日：2006-05-08
Applicant: Jean Montagu , Roger Dowd , David Root
Inventor： Jean Montagu , Roger Dowd , David Root
CPC classification number: G01N33/544 , G01N33/54366
Abstract: An immobilizing device for biological material comprises a rigid support (12) carrying a substrate layer (20, 20′) of polymer having biological immobilizing properties, e.g. for amino and nucleic acids. Substantially solid ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably between about 0.1 and 0.5 micron, and micro-porous, ultra-thin substrate layers (20′) having a thickness less than about 5 micron, preferably less than 3 micron, 2 or 1 micron are shown, which may be segmented by isolating moats M. The substrate layer is on a microscope slide (302), round disc (122), bio-cassette, at the bottom of a well of a multiwell plate, and as a coating inside a tube. Fluorescence or luminescence intensity and geometric calibration spots (420) are shown. Reading is enhanced by the intensity calibration spots. (420) to enable normalization of readings under uneven illumination conditions, as when reading by dark field, side illumination mode. The reference spots are shown being printed simultaneously with printing an array of biological spots or with the same equipment. Methods of forming layers of the device include controlled drawing from a bath of coating composition and drying, and spinning of C-D shaped substrates. Post-forming treatment is shown by corona treatment and radiation. Adherent metal oxides (14), silica-based materials and other materials are used to unite layers of the composite. In multi-well plates the oxide promotes joining of a bottom plate (95, 95′) and upper, well-defining structure (94) of dissimilar material. The oxides (14) also provide beneficial opacity to prevent light entering the glass support, for applying potential to the substrate, etc.
Abstract translation: 用于生物材料的固定装置包括承载具有生物固定性质的聚合物的基底层（20,20'）的刚性支撑体（12），例如。 用于氨基和核酸。 具有厚度小于约5微米，优选约0.1至0.5微米的基本上固体的超薄基底层（20'）和厚度小于约5微米的微孔超薄基底层（20'） ，优选小于3微米，2或1微米，其可以通过分离护羊M分段。基底层在显微镜载玻片（302），圆盘（122），生物盒，底部 多孔板，以及管内的涂层。 显示荧光或发光强度和几何校准点（420）。 通过强度校准点增强读数。 （420），以便在不均匀照射条件下使读数标准化，如通过暗场，侧面照明模式读取。 参考点被显示为同时印刷生物斑点阵列或用相同的设备打印。 形成装置层的方法包括从涂料组合物的浴中进行控制拉伸和干燥，以及C-D形基材的纺丝。 后处理通过电晕处理和辐射显示。 使用粘附金属氧化物（14），二氧化硅基材料和其他材料来组合复合材料层。 在多孔板中，氧化物促进底板（95,95'）和不同材料的上部，良好限定结构（94）的接合。 氧化物（14）还提供有益的不透明性，以防止光进入玻璃支撑体，以将电位施加到基底等。
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Patent Agency Ranking
公开(公告)号：US20060161409A1
公开(公告)日：2006-07-20
申请号：US11035651
申请日：2005-01-14
Applicant: David Root , David Sharrit , John Wood
Inventor： David Root , David Sharrit , John Wood
IPC: G06F17/50
CPC classification number: G06F17/5036
Abstract: A model of a device is generated. An input port of the device is stimulated with a large amplitude signal having a central frequency. A first port of the device is perturbed with a small amplitude signal tone. The small amplitude signal tone is at a frequency offset slightly from a harmonic of the central frequency. Spectral component frequencies of a resulting signal from the device are obtained to determine model coefficients for the device. At least some of the spectral component frequencies occur at frequencies offset slightly from harmonics of the central frequency.
Abstract translation: 生成设备的型号。 设备的输入端口被具有中心频率的大振幅信号激励。 设备的第一个端口受到小振幅信号音的干扰。 小振幅信号音频率偏离中心频率谐波的频率偏移。 获得来自装置的结果信号的频谱分量频率以确定装置的模型系数。 至少一些频谱分量频率以与中心频率的谐波略微偏移的频率出现。
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公开(公告)号：US5466602A
公开(公告)日：1995-11-14
申请号：US180695
申请日：1994-01-13
Applicant: George Lyman , Gregory Mathus , David Root
Inventor： George Lyman , Gregory Mathus , David Root
Abstract: The present invention relates to an apparatus for growing tissue culture in vitro. The apparatus features a housing which fits into a well containing media. A bottom membrane surface is maintained at a depth within the well, and the housing centered in the well by a flange and rim which cooperate with the well.
Abstract translation: 本发明涉及体外培养组织培养的装置。 该装置具有适合于容纳介质的容器的壳体。 底部膜表面保持在井内的深度，并且壳体通过与井配合的凸缘和边缘在井中居中。
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公开(公告)号：US5215920A
公开(公告)日：1993-06-01
申请号：US695300
申请日：1991-05-03
Applicant: George Lyman , Gregory Mathus , David Root
Inventor： George Lyman , Gregory Mathus , David Root
Abstract: Apparatus for growing tissue cultures in vitro, which permits a concentration gradient of nutrients to develop through a permeable membrane to which a sample of tissue is attached. The permeable membrane is attached to the bottom end of a tubular support that in turn hangs by a flange connected to its upper end on the top of a well containing the nutrients. Typically, the well is part of a tissue culture cluster dish. The flange of the support positions the support and membrane centrally in the well so as to avoid capillary action in the space between the well and support. The configuration of the support and its cooperation with the lid of the cluster dish also prevents the support and membrane from floating in the nutrient solution in the well. Openings in the support provide access for a pipette to add and withdraw fluid from the space between the well and membrane support and from the space below the membrane.
Abstract translation: 用于在体外生长组织培养物的装置，其允许营养物的浓度梯度通过附着有组织样品的渗透膜发育。 可渗透膜附接到管状支撑件的底端，管状支撑件的底端又通过连接到容纳营养物质的孔的顶部上方的凸缘而悬挂。 通常，孔是组织培养簇盘的一部分。 支撑件的凸缘将支撑件和膜中心定位在井中，以避免在井和支撑件之间的空间中的毛细作用。 支架的配置及其与簇盘盖的配合也可防止支撑和膜浮在井中的营养液中。 支架中的开口提供移液管的通道，以从孔和膜支撑之间的空间以及膜下方的空间添加和抽出流体。
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公开(公告)号：US20080103059A1
公开(公告)日：2008-05-01
申请号：US12001850
申请日：2007-12-13
Applicant: Brian Webb , Jinlin Peng , Michael Brady , Mircea Despa , Keith Horn , Joydeep Lahiri , David Root , James Stamatoff
Inventor： Brian Webb , Jinlin Peng , Michael Brady , Mircea Despa , Keith Horn , Joydeep Lahiri , David Root , James Stamatoff
IPC: C40B30/04
CPC classification number: B01L3/50853 , B01J2219/00317 , B01J2219/00504 , B01J2219/00511 , B01J2219/00585 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00653 , B01J2219/00662 , B01J2219/00707 , B01L3/5025 , B01L2200/025 , B01L2200/026 , B01L2300/046 , B01L2300/0636 , B01L2300/0829 , C12Q1/6837 , C40B60/14 , G01N33/54366 , G01N33/54373 , G01N33/54386 , G01N35/028
Abstract: A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity.
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公开(公告)号：US20070141594A1
公开(公告)日：2007-06-21
申请号：US11546019
申请日：2006-10-11
Applicant: Biao Luo , David Root , Xiaoping Yang , Gregory Hinkle
Inventor： Biao Luo , David Root , Xiaoping Yang , Gregory Hinkle
CPC classification number: C12N15/1093 , C12N15/111 , C12N2310/14 , C12N2310/53 , C12N2330/31
Abstract: Described herein is a method of cloning synthetic oligos (including in situ synthesized oligos) into an (one or more) expression vector for library (e.g., shRNA library) production. The oligos are synthesized with one portion of the first stem of the hairpin, followed by a first loop sequence, the complete second stem, a second loop sequence, and finished with the remaining portion of the first stem of the hairpin. The two portions of the first stem anneal to the second stem, juxtaposing the 5′ end close to the 3′ end of the oligo. The methods described herein selected for hairpins with perfectly base-paired stems. After annealing, a ligase is added to the annealed oligos and the base-paired hairpins are preferentially annealed, and ligated, creating closed circular oligos. The now circularized hairpins served as templates for rolling circle amplification using a polymerase with high processivity. One or more primers complementary to the two strands of the amplified double stranded circular hairpins initiate the rolling circle amplification in the presence of a polymerase. Using primers (e.g., a sense and antisense primer), the rolling circle amplification yields double stranded hairpin sequences. These can be digested (e.g., using restriction enzymes) to produce a double-stranded hairpin fragment encoding a single hairpin. The fragment can be cloned into an appropriately digested vector for a variety of uses including expression.
Abstract translation: 本文描述了将合成寡核苷酸（包括原位合成的寡核苷酸）克隆到（一个或多个）用于文库（例如shRNA文库）生产的表达载体中的方法。 寡核苷酸与发夹的第一茎的一部分合成，随后是第一环序列，完整的第二茎，第二环序列，并用发夹的第一茎的剩余部分完成。 第一茎的两个部分与第二茎退火，将5'末端与寡核苷酸的3'末端并列。 本文所述的方法选择用于具有完全碱基配对茎的发夹。 退火后，将连接酶加入到退火的寡核苷酸中，并且将碱基配对的发夹优先退火并连接，产生闭合的环状寡核苷酸。 现在的圆形发夹作为使用高活性的聚合酶进行滚环扩增的模板。 与扩增的双链环状发夹的两条链互补的一个或多个引物在聚合酶存在下引发滚环扩增。 使用引物（例如有义和反义引物），滚环扩增产生双链发夹序列。 这些可以被消化（例如，使用限制酶）以产生编码单个发夹的双链发夹片段。 可以将片段克隆到适当消化的载体中，用于多种用途，包括表达。
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