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    Energetically activated biomedical nanotheurapeutics integrating dental and medical treatments and procedures
    1.
    发明申请
    Energetically activated biomedical nanotheurapeutics integrating dental and medical treatments and procedures 审中-公开
    积极激活的生物医学纳米治疗，整合牙科和医疗治疗和程序

    公开(公告)号：US20080070195A1

    公开(公告)日：2008-03-20

    申请号：US11895404

    申请日：2007-08-24

    申请人： Enrico DiVito ,  Douglas Glover ,  Kemmons Tubbs

    发明人： Enrico DiVito ,  Douglas Glover ,  Kemmons Tubbs

    IPC分类号： A61C5/02

    CPC分类号： A61C5/50 ,  A61C5/40

    摘要： A method that includes the following effects: the ability to expand, porositize, clean, decontaminate, debride, break-down and/or destroys, or aids and accentuates the dissolution or extraction of biological structures, nerves and tissues. The methodology uses nano-technology and/or other chemistries may or may not be excited by energy sources to expand and porositize the tissue, which enhances the penetration and effectiveness and acceleration of subsequent chemical, mechanical, and/or procedural steps in the protocol or process. Furthermore, the method includes the ability to associate with and/or initiate in the reconstructive growth and healing process associated with healthy tissue after surgery and/or the ability to destroy diseased or necrotic tissues or cells. The method also includes the ability to begin, construct or stage the activation of cells and/or tissues, including the area of transplantation and use in stem or primordial cell accentuation, their attachment and/or stimulation for growth and differentiation.

    摘要翻译： 一种包括以下效果的方法：扩展，固化，清洁，净化，清除，分解和/或破坏的能力，或辅助和强调生物结构，神经和组织的溶解或提取。 该方法使用纳米技术和/或其他化学物质可能激发或可能不被能量来激发，以扩大和增加组织的孔隙度，这增强了协议中随后的化学，机械和/或程序步骤的穿透和有效性和加速， 处理。 此外，该方法包括与在手术后与健康组织相关的重建生长和愈合过程中和/或破坏患病或坏死组织或细胞的能力缔合和/或启动的能力。 该方法还包括开始，构建或分化细胞和/或组织的活化的能力，包括在茎或原始细胞突变中的移植和使用的区域，其附着和/或刺激生长和分化的能力。

    METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS
    5.
    发明申请
    METHOD AND APPARATUS FOR MASS SPECTROMETRIC IMMUNOASSAY ANALYSIS OF SPECIFIC BIOLOGICAL FLUID PROTEINS 审中-公开
    特异性生物流体蛋白质质谱免疫分析方法与装置

    公开(公告)号：US20070128734A1

    公开(公告)日：2007-06-07

    申请号：US11671576

    申请日：2007-02-06

    申请人： Urban Kiernan ,  Eric Niederkofler ,  Kemmons Tubbs ,  Dobrin Nedelkov ,  Randall Nelson

    发明人： Urban Kiernan ,  Eric Niederkofler ,  Kemmons Tubbs ,  Dobrin Nedelkov ,  Randall Nelson

    IPC分类号： G01N33/543

    CPC分类号： G01N33/6803

    摘要： Presented herein are methods, devices and kits for the mass spectrometric immunoassay (MSIA) of proteins and their variants that are present in complex biological fluids or extracts. Protein and variant levels can be determined using quantitative methods in which the protein/variant signals are normalized to signals of internal reference standard species (either doped into the samples prior to the MSIA analysis, or other endogenous protein co-extracted with the target proteins) and the values compared to working curves constructed from samples containing known concentrations of the protein or variants.

    摘要翻译： 本文介绍的是存在于复杂生物液体或提取物中的蛋白质及其变体的质谱法免疫测定（MSIA）的方法，装置和试剂盒。 蛋白质和变体水平可以使用定量方法来确定，其中蛋白质/变体信号被归一化为内部参考标准物质的信号（在MSIA分析前掺入样品或与靶蛋白共提取的其它内源性蛋白质） 并且与从含有已知浓度的蛋白质或变体的样品构建的工作曲线相比较的值。

    Analysis of insulin-like growth factors from biological fluids by the use of affinity-based mass spectrometric methods
    6.
    发明申请
    Analysis of insulin-like growth factors from biological fluids by the use of affinity-based mass spectrometric methods 审中-公开
    通过使用基于亲和力的质谱法分析来自生物液体的胰岛素样生长因子

    公开(公告)号：US20080064044A9

    公开(公告)日：2008-03-13

    申请号：US10756100

    申请日：2004-01-12

    申请人： Randall Nelson ,  Dobrin Nedelkov ,  Urban Kiernan ,  Kemmons Tubbs

    发明人： Randall Nelson ,  Dobrin Nedelkov ,  Urban Kiernan ,  Kemmons Tubbs

    IPC分类号： G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/54373 ,  G01N33/6851 ,  G01N2333/475

    摘要： Presented herein are affinity-based mass spectrometric methods and assays for analysis of insulin like growth factors 1 and 2 (IGF-1 and IGF-2) present in complex biological mixtures and fluids. IGF-1 and IGF-2 were assayed from human plasma via BIA/MS, utilizing antibodies as ligands for affinity retrieval. Detection of both targeted and non-targeted IGFs in the mass spectra indicated possible protein complex retrieval by the individual antibodies. Plasma samples were investigated under variable denaturing conditions to confirm the detection of both free and bound IGFs. In a MSIA approach to IGF detection, pipettor tips containing porous solid supports covalently derivatized with anti-IGF antibodies were used to extract specific IGFs from plasma in preparation for mass spectrometry. Single or multiplex IGF-1 and IGF-2 assays were performed, resulting in detection of wild-type IGF-1 and 2, and a truncated IGF-2 variant, missing its N-terminal Alanine (also detected in the BIA/MS experiments). IGF-1 was quantified from several individuals via the use of internal reference standard species (rat IGF-1, doped into the samples prior to the MSIA analysis) and a working curve constructed from samples containing known concentrations of IGF-1.

    摘要翻译： 本文提出了基于亲和力的质谱法和用于分析复合生物混合物和流体中存在的胰岛素样生长因子1和2（IGF-1和IGF-2）的分析方法。 通过BIA / MS从人血浆中检测IGF-1和IGF-2，利用抗体作为亲和力检索的配体。 在质谱中检测靶向和非靶向IGFs表明可能的单个抗体的蛋白质复合物检索。 在可变变性条件下研究血浆样品，以确认游离和结合的IGF的检测。 在用于IGF检测的MSIA方法中，使用含有用抗IGF抗体共价衍生的多孔固体支持物的移液器尖端来提取来自血浆的特异性IGF，以进行质谱分析。 进行单次或多重IGF-1和IGF-2测定，导致野生型IGF-1和2以及截短的IGF-2变体的检测，缺失其N-末端丙氨酸（也在BIA / MS实验中检测到 ）。 通过使用内部参考标准物质（大鼠IGF-1，在MSIA分析之前掺入样品中）从几个个体定量IGF-1和从含有已知浓度的IGF-1的样品构建的工作曲线。

    Analysis of insulin-like growth factors from biological fluids by the use of affinity-based mass spectrometric methods
    8.
    发明申请
    Analysis of insulin-like growth factors from biological fluids by the use of affinity-based mass spectrometric methods 审中-公开
    通过使用基于亲和力的质谱法分析来自生物液体的胰岛素样生长因子

    公开(公告)号：US20050032116A1

    公开(公告)日：2005-02-10

    申请号：US10756100

    申请日：2004-01-12

    申请人： Randall Nelson ,  Dobrin Nedelkov ,  Urban Kiernan ,  Kemmons Tubbs

    发明人： Randall Nelson ,  Dobrin Nedelkov ,  Urban Kiernan ,  Kemmons Tubbs

    IPC分类号： C12M1/34 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

    CPC分类号： G01N33/54373 ,  G01N33/6851 ,  G01N2333/475

    摘要： Presented herein are affinity-based mass spectrometric methods and assays for analysis of insulin like growth factors 1 and 2 (IGF-1 and IGF-2) present in complex biological mixtures and fluids. IGF-1 and IGF-2 were assayed from human plasma via BIA/MS, utilizing antibodies as ligands for affinity retrieval. Detection of both targeted and non-targeted IGFs in the mass spectra indicated possible protein complex retrieval by the individual antibodies. Plasma samples were investigated under variable denaturing conditions to confirm the detection of both free and bound IGFs. In a MSIA approach to IGF detection, pipettor tips containing porous solid supports covalently derivatized with anti-IGF antibodies were used to extract specific IGFs from plasma in preparation for mass spectrometry. Single or multiplex IGF-1 and IGF-2 assays were performed, resulting in detection of wild-type IGF-1 and 2, and a truncated IGF-2 variant, missing its N-terminal Alanine (also detected in the BIA/MS experiments). IGF-1 was quantified from several individuals via the use of internal reference standard species (rat IGF-1, doped into the samples prior to the MSIA analysis) and a working curve constructed from samples containing known concentrations of IGF-1.

    摘要翻译： 本文提出了基于亲和力的质谱法和用于分析复合生物混合物和流体中存在的胰岛素样生长因子1和2（IGF-1和IGF-2）的分析方法。 通过BIA / MS从人血浆中检测IGF-1和IGF-2，利用抗体作为亲和力检索的配体。 在质谱中检测靶向和非靶向IGFs表明可能的单个抗体的蛋白质复合物检索。 在可变变性条件下研究血浆样品，以确认游离和结合的IGF的检测。 在用于IGF检测的MSIA方法中，使用含有用抗IGF抗体共价衍生的多孔固体支持物的移液器尖端来提取来自血浆的特异性IGF，以进行质谱分析。 进行单次或多重IGF-1和IGF-2测定，导致野生型IGF-1和2以及截短的IGF-2变体的检测，缺失其N-末端丙氨酸（也在BIA / MS实验中检测到 ）。 通过使用内部参考标准物质（大鼠IGF-1，在MSIA分析之前掺入样品中）从几个个体定量IGF-1和从含有已知浓度的IGF-1的样品构建的工作曲线。