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17 条记录 (耗时 0.022 秒)

    Fluorescent dyes and methods of use thereof
    1.
    发明授权
    Fluorescent dyes and methods of use thereof 有权

    公开(公告)号：US10106573B2

    公开(公告)日：2018-10-23

    申请号：US15205438

    申请日：2016-07-08

    申请人： ENZO LIFE SCIENCES, INC.

    发明人： Zaiguo Li ,  Praveen Pande

    IPC分类号： C07H21/00 ,  C07D311/82 ,  C07H19/04 ,  C12Q1/70 ,  C09B11/24 ,  C09B11/02 ,  C09B11/06

    摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

    Processes and kits for determining multi-drug resistance of cells
    6.
    发明授权
    Processes and kits for determining multi-drug resistance of cells 有权
    确定细胞多药耐药的方法和试剂盒

    公开(公告)号：US09097673B2

    公开(公告)日：2015-08-04

    申请号：US13864524

    申请日：2013-04-17

    申请人： ENZO LIFE SCIENCES, INC.

    发明人： Irina Lebedeva ,  Praveen Pande ,  Wayne Forrest Patton

    IPC分类号： C12M1/34 ,  G01N21/64 ,  G01N33/50

    CPC分类号： G01N21/6486 ,  G01N33/502 ,  G01N2800/44

    摘要： This invention relates to multi-drug resistance (MDR) in cells, and the use of certain xanthene compounds for determining drug resistance in cells and the effect of test compounds on cell membrane transport by the membrane transporters MDR1, MRP and BCRP. Processes and kits for making these determinations and measuring these effects are described and provided.

    摘要翻译： 本发明涉及细胞中的多药耐药性（MDR），以及某些呫吨化合物用于测定细胞耐药性的用途以及受试化合物对膜转运体MDR1，MRP和BCRP对细胞膜转运的影响。 描述和提供了用于进行这些测定和测量这些影响的方法和试剂盒。

    Fluorescent dyes
    7.
    发明授权
    Fluorescent dyes 有权
    荧光染料

    公开(公告)号：US08674102B2

    公开(公告)日：2014-03-18

    申请号：US13733239

    申请日：2013-01-03

    申请人： Enzo Life Sciences, Inc.

    发明人： Praveen Pande ,  Zaiguo Li ,  Maciej Szczepanik ,  Wayne Forrest Patton

    IPC分类号： C07F9/28 ,  C12Q1/00

    CPC分类号： C09B23/164 ,  C09B23/0066 ,  C09B23/0075 ,  C09B23/0091 ,  C09B23/04 ,  C09B23/06 ,  C09B23/083 ,  C09B23/105 ,  C09B23/145 ,  C09B23/146 ,  C12Q1/68 ,  G01N33/583

    摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.

    摘要翻译： 本发明提供可用于检测或定量所需靶分子（例如蛋白质，核酸和细胞器器）的染料，活性染料和标记试剂。 提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。 还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。 已经对本发明的新型染料进行了改进以提供有益的性质。

    FLUORESCENT DYES AND METHODS OF USE THEREOF
    9.
    发明申请
    FLUORESCENT DYES AND METHODS OF USE THEREOF 有权

    公开(公告)号：US20210079034A1

    公开(公告)日：2021-03-18

    申请号：US16952399

    申请日：2020-11-19

    申请人： Enzo Life Sciences, Inc.

    发明人： Zaiguo Li ,  Praveen Pande

    IPC分类号： C07H21/00 ,  C12Q1/70 ,  C09B11/24 ,  C07H19/04 ,  C09B11/02 ,  C09B11/06

    摘要： Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

    SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION
    10.
    发明申请
    SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION 审中-公开

    公开(公告)号：US20190119719A1

    公开(公告)日：2019-04-25

    申请号：US16177676

    申请日：2018-11-01

    申请人： ENZO LIFE SCIENCES, INC.

    发明人： Irina Lebedeva ,  Wayne Forrest Patton ,  Maciej Szczepanik ,  Yuejun Xiang ,  Praveen Pande

    IPC分类号： C12Q1/26 ,  C12Q1/48 ,  C07D311/08 ,  C07D491/052 ,  C07D491/20 ,  C09K11/06 ,  C12Q1/44 ,  C12Q1/37 ,  C07D493/10

    摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.