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公开(公告)号：US10718005B2
公开(公告)日：2020-07-21
申请号：US16177676
申请日：2018-11-01
IPC分类号： C12Q1/26 , C09K11/06 , C12Q1/37 , C12Q1/44 , C12Q1/48 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10
摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.
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公开(公告)号：US08674102B2
公开(公告)日：2014-03-18
申请号：US13733239
申请日：2013-01-03
CPC分类号： C09B23/164 , C09B23/0066 , C09B23/0075 , C09B23/0091 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/145 , C09B23/146 , C12Q1/68 , G01N33/583
摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.
摘要翻译： 本发明提供可用于检测或定量所需靶分子（例如蛋白质，核酸和细胞器器）的染料，活性染料和标记试剂。 提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。 还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。 已经对本发明的新型染料进行了改进以提供有益的性质。
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公开(公告)号：US20190119719A1
公开(公告)日：2019-04-25
申请号：US16177676
申请日：2018-11-01
IPC分类号： C12Q1/26 , C12Q1/48 , C07D311/08 , C07D491/052 , C07D491/20 , C09K11/06 , C12Q1/44 , C12Q1/37 , C07D493/10
摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.
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公开(公告)号：US09574222B2
公开(公告)日：2017-02-21
申请号：US14447817
申请日：2014-07-31
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： Provided is a compound comprising the structure: (SIG)-(SI-MOD)m. In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.
摘要翻译： 在该化合物中，SIG是信号分子，SI是与SIG结合的自消除结构，使得SIG相对于没有SI的SIG的信号具有降低的信号，MOD是与SI结合的部分，其经受 活化剂，m为1至约10的整数。用该化合物，当MOD被活化剂修饰时，SI是不稳定的并且从SIG自我切割，使得SIG产生增加的信号。 还提供了使用上述化合物来确定样品是否包含活化剂的方法。 另外提供了使用上述硝基还原酶是活化剂的化合物来确定细胞是否包含硝基还原酶的方法。 还提供了使用上述硝基还原酶是活化剂的化合物来确定哺乳动物细胞是否缺氧的方法。 还提供了使用上述硝基还原酶为活化剂的化合物检测包含硝基还原酶的微生物的方法。 还提供了使用上述硝酸还原酶是活化剂的化合物来鉴定样品中的硝基还原酶的方法。
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公开(公告)号：US11073512B2
公开(公告)日：2021-07-27
申请号：US16202213
申请日：2018-11-28
发明人： Jack Coleman , Maciej Szczepanik , Richard Jin
IPC分类号： G01N33/53 , C07H21/00 , C07H21/04 , C09B11/08 , C09B11/24 , C12Q1/6818 , G01N33/58 , G01N33/82 , G01N33/94
摘要： Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
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公开(公告)号：US08604204B2
公开(公告)日：2013-12-10
申请号：US13733324
申请日：2013-01-03
IPC分类号： C07D417/02 , C12Q1/00
CPC分类号： C09B23/164 , C09B23/0066 , C09B23/0075 , C09B23/0091 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/145 , C09B23/146 , C12Q1/68 , G01N33/583
摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.
摘要翻译： 本发明提供可用于检测或定量所需靶分子（例如蛋白质，核酸和细胞器器）的染料，活性染料和标记试剂。 提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。 还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。 已经对本发明的新型染料进行了改进以提供有益的性质。
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