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公开(公告)号:US08722059B2
公开(公告)日:2014-05-13
申请号:US13214110
申请日:2011-08-19
申请人: Erich Hoffman , Hong Jin , Bin Lu , Greg Duke , George Kemble
发明人: Erich Hoffman , Hong Jin , Bin Lu , Greg Duke , George Kemble
IPC分类号: A61K39/145 , C12N7/00 , C12N7/04
CPC分类号: A61K39/145 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2760/16122 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16243 , C12N2760/16262
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。
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公开(公告)号:US08012736B2
公开(公告)日:2011-09-06
申请号:US10423828
申请日:2003-04-25
申请人: Erich Hoffman , Hong Jin , Bin Lu , Greg Duke , George Kemble
发明人: Erich Hoffman , Hong Jin , Bin Lu , Greg Duke , George Kemble
IPC分类号: C12N7/02
CPC分类号: A61K39/145 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2760/16122 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16243 , C12N2760/16262
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。
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公开(公告)号:US20120020997A1
公开(公告)日:2012-01-26
申请号:US13214110
申请日:2011-08-19
申请人: Erich Hoffman , Hong Jin , Bin Lu , Greg Duke , George Kemble
发明人: Erich Hoffman , Hong Jin , Bin Lu , Greg Duke , George Kemble
IPC分类号: A61K39/145 , C12N7/04 , C12N7/00 , C07K14/11 , C40B40/08 , C12N5/00 , C12N7/01 , C07H21/04 , C12N7/02 , C12N15/86
CPC分类号: A61K39/145 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N15/86 , C12N15/87 , C12N2760/16122 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16243 , C12N2760/16262
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。
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公开(公告)号:US20090175907A1
公开(公告)日:2009-07-09
申请号:US12254131
申请日:2008-10-20
申请人: Erich Hoffman , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
发明人: Erich Hoffman , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
IPC分类号: A61K39/145 , C12N7/01 , C12N15/74 , A61P31/16
CPC分类号: C12N15/86 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2760/16122 , C12N2760/16134 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16234 , C12N2760/16243 , C12N2760/16262 , C12N2840/20
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外,本发明提供了生产能够在含胚鸡蛋和/或细胞(例如Vero和/或MDCK)中复制的流感病毒的方法,并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应(ca)流感病毒的方法(和包含其的免疫原性组合物)。
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公开(公告)号:US08114415B2
公开(公告)日:2012-02-14
申请号:US12254131
申请日:2008-10-20
申请人: Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
发明人: Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
IPC分类号: A61K39/145 , A61K39/00 , A61K39/12 , C12P21/04
CPC分类号: C12N15/86 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2760/16122 , C12N2760/16134 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16234 , C12N2760/16243 , C12N2760/16262 , C12N2840/20
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外,本发明提供了生产能够在含胚鸡蛋和/或细胞(例如Vero和/或MDCK)中复制的流感病毒的方法,并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应(ca)流感病毒的方法(和包含其的免疫原性组合物)。
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公开(公告)号:US07465456B2
公开(公告)日:2008-12-16
申请号:US11133345
申请日:2005-05-20
申请人: Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
发明人: Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
IPC分类号: A61K39/145 , C12Q1/70 , C12P19/34
CPC分类号: C12N15/86 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2760/16122 , C12N2760/16134 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16234 , C12N2760/16243 , C12N2760/16262 , C12N2840/20
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外,本发明提供了生产能够在含胚鸡蛋和/或细胞(例如Vero和/或MDCK)中复制的流感病毒的方法,并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应(ca)流感病毒的方法(和包含其的免疫原性组合物)。
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公开(公告)号:US20050266026A1
公开(公告)日:2005-12-01
申请号:US11133345
申请日:2005-05-20
申请人: Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
发明人: Erich Hoffmann , Hong Jin , Bin Lu , Gregory Duke , George Kemble , Zhongying Chen
CPC分类号: C12N15/86 , A61K2039/5254 , C07K14/005 , C12N7/00 , C12N15/85 , C12N2760/16122 , C12N2760/16134 , C12N2760/16143 , C12N2760/16162 , C12N2760/16222 , C12N2760/16234 , C12N2760/16243 , C12N2760/16262 , C12N2840/20
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外,本发明提供了生产能够在含胚鸡蛋和/或细胞(例如Vero和/或MDCK)中复制的流感病毒的方法,并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应(ca)流感病毒的方法(和包含其的免疫原性组合物)。
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公开(公告)号:US08354114B2
公开(公告)日:2013-01-15
申请号:US12090435
申请日:2006-10-17
IPC分类号: A61K39/145 , A61K39/285
CPC分类号: C12N7/00 , A61K35/76 , A61K38/00 , A61K39/12 , A61K39/145 , A61K39/295 , A61K2039/5254 , C07K14/005 , C12N15/86 , C12N2760/16122 , C12N2760/16134 , C12N2760/16143 , C12N2760/16151 , C12N2760/16222 , C12N2760/16234 , C12N2760/16243 , C12N2760/16251
摘要: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics.
摘要翻译: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供用于多质粒流感病毒表达系统的双向表达载体。 另外,本发明提供了生产能够在含胚鸡蛋和/或细胞(例如Vero和/或MDCK)中复制的流感病毒的方法,并进一步提供具有增强的复制特征的流感病毒。
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公开(公告)号:US20120282673A1
公开(公告)日:2012-11-08
申请号:US13474409
申请日:2012-05-17
申请人: Hong JIN , Robert Brazas , Bin Lu
发明人: Hong JIN , Robert Brazas , Bin Lu
IPC分类号: C12N7/01
CPC分类号: C12N7/045 , A61K39/12 , A61K39/155 , A61K2039/5254 , A61K2039/543 , C07K14/005 , C12N7/00 , C12N15/86 , C12N2760/18522 , C12N2760/18534 , C12N2760/18543 , C12N2760/18561 , C12N2800/40 , G01N33/56983 , G01N2469/20 , G01N2500/00
摘要: The present invention provides recombinant respiratory syncytial viruses that have an attenuated phenotype and that comprise one or more mutations in the viral P, M2-1 and/or M2-2 proteins, as well as live attenuated vaccines comprising such viruses and nucleic acids encoding such viruses. Recombinant RSV P, M2-1 and M2-2 proteins are described. Methods of producing attenuated recombinant RSV, and methods of quantitating neutralizing antibodies that utilize recombinant viruses of family Paramyxoviridae, are also provided.
摘要翻译: 本发明提供具有减毒表型并且包含病毒P,M2-1和/或M2-2蛋白中的一个或多个突变的重组呼吸道合胞病毒,以及包含这样的病毒的活减毒疫苗和编码这种病毒的核酸 病毒 描述了重组RSV P,M2-1和M2-2蛋白。 还提供了产生减毒重组RSV的方法,以及定量利用副粘病毒科重组病毒的中和抗体的方法。
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公开(公告)号:US20100190232A1
公开(公告)日:2010-07-29
申请号:US12537288
申请日:2009-08-07
申请人: Hong Jin , Robert Brazas , Bin Lu
发明人: Hong Jin , Robert Brazas , Bin Lu
IPC分类号: C12N7/01
CPC分类号: C12N7/045 , A61K39/12 , A61K39/155 , A61K2039/5254 , A61K2039/543 , C07K14/005 , C12N7/00 , C12N15/86 , C12N2760/18522 , C12N2760/18534 , C12N2760/18543 , C12N2760/18561 , C12N2800/40 , G01N33/56983 , G01N2469/20 , G01N2500/00
摘要: The present invention provides recombinant respiratory syncytial viruses that have an attenuated phenotype and that comprise one or more mutations in the viral P, M2-1 and/or M2-2 proteins, as well as live attenuated vaccines comprising such viruses and nucleic acids encoding such viruses. Recombinant RSV P, M2-1 and M2-2 proteins are described. Methods of producing attenuated recombinant RSV, and methods of quantitating neutralizing antibodies that utilize recombinant viruses of family Paramyxoviridae, are also provided.
摘要翻译: 本发明提供具有减毒表型并且包含病毒P,M2-1和/或M2-2蛋白中的一个或多个突变的重组呼吸道合胞病毒,以及包含这样的病毒的活减毒疫苗和编码这种病毒的核酸 病毒 描述了重组RSV P,M2-1和M2-2蛋白。 还提供了产生减毒重组RSV的方法,以及定量利用副粘病毒科重组病毒的中和抗体的方法。
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