Hybridization promotion reagents
    4.
    发明授权
    Hybridization promotion reagents 失效
    杂交促进试剂

    公开(公告)号:US5589335A

    公开(公告)日:1996-12-31

    申请号:US290001

    申请日:1994-08-11

    IPC分类号: C12Q1/68

    摘要: Novel reagents are provided along with methods for their use in nucleic acid hybridization assays for detecting target nucleic acids in samples. The most preferred reagent is a mixture of guanidinium thiocyanate and tetramethylammonium:Trifluoracetate and possesses many properties for facilitating hybridization between target nucleic acid and nucleic acid probes capable of binding thereto and surprisingly results in the phenomenon of superstoichiometric labeling.

    摘要翻译: 提供了新的试剂以及用于检测样品中靶核酸的核酸杂交测定中使用的方法。 最优选的试剂是硫氰酸胍和四甲基铵:三氟乙酸盐的混合物,并且具有许多促进靶核酸与能够结合到其上的核酸探针之间的杂交的性质,并且令人惊讶地导致超化学计量标记的现象。