公开(公告)号：US20150175969A1

公开(公告)日：2015-06-25

申请号：US14640218

申请日：2015-03-06

Applicant: FUJIFILM Corporation

Inventor： Yuichi YOSHINO ,  Rie IWATA ,  Kentaro NAKAMURA

IPC: C12N5/077 ,  C07K14/78

CPC classification number: C12N5/0655 ,  A61K38/00 ,  A61L27/227 ,  A61L2300/252 ,  A61L2430/06 ,  C07K14/78 ,  C12N2501/998

Abstract: A polypeptide having an amino acid sequence in which the number of RGD sequences contained per molecular weight of 10 kDa is not less than 0.30; the number of GFPGER sequences contained per molecular weight of 10 kDa is not less than 0.15; and the number of GVMGFP sequences contained per molecular weight of 10 kDa is less than 0.30; is provided. A scaffold composition, a composition for repairing a cartilage tissue, a composition for culturing cartilage cells, and a composition for promoting glycosaminoglycan production, which compositions contain the above polypeptide, are also provided.

Abstract translation: 具有每分子量为10kDa的RGD序列数不少于0.30的氨基酸序列的多肽; 每分子量为10kDa的GFPGER序列数不低于0.15; 每分子量为10kDa的GVMGFP序列数小于0.30; 被提供。 还提供了一种支架组合物，用于修复软骨组织的组合物，用于培养软骨细胞的组合物和用于促进糖胺聚糖生产的组合物，该组合物含有上述多肽。

公开(公告)号：US20240191294A1

公开(公告)日：2024-06-13

申请号：US18410502

申请日：2024-01-11

Applicant: FUJIFILM Corporation

Inventor： Kentaro NAKAMURA ,  Tsukasa KITAHASHI ,  Yuichi YOSHINO ,  Ryo KOGAWA

IPC: C12Q1/6876 ,  A61K35/28 ,  A61P19/02

CPC classification number: C12Q1/6876 ,  A61K35/28 ,  A61P19/02 ,  C12Q2600/158

Abstract: An object of the present invention is to provide such a quality management method for a cell that enables the sorting-out of a cell having drug efficacy, and a method of producing a cell using the quality management method. According to the present invention, there is provided a method that is a quality management method for a cell for a medical treatment or drug discovery, the method including managing a behavior of one or more specific protein molecules, which is essential for exhibiting a therapeutic effect due to a cell and managing an expression behavior of a related gene group that is characteristic of the cell.

公开(公告)号：US20160251613A1

公开(公告)日：2016-09-01

申请号：US15064619

申请日：2016-03-09

Applicant: FUJIFILM CORPORATION

Inventor： Yuta MURAKAMI ,  Sanae NOMIYAMA ,  Keita HAGIYA ,  Yuichi YOSHINO ,  Rie HANDO ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/00 ,  C12N5/074

CPC classification number: C12N5/0068 ,  C07K7/06 ,  C07K14/00 ,  C07K14/78 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2533/30 ,  C12N2533/50

Abstract: A culture method for pluripotent stem cells includes culturing pluripotent stem cells on a cell culture surface of a support by using a medium in which the concentration of 2-mercaptoethano is equal to or less than 10 μM in the presence of a polypeptide consisting of 40 to 450 amino acid residues, in which the polypeptide includes (1) a first domain including at least one amino acid sequence selected from the group consisting of an amino acid sequence represented by CSYYQSC (SEQ ID NO: 1) and an amino acid sequence represented by RGD and (2) a second domain including (2-i) an amino acid sequence which is represented by PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN (SEQ ID NO: 2), (2-ii) an amino acid sequence which shares sequence identity of equal to or higher than 50% with the amino acid sequence represented by SEQ ID NO: 2 and exhibits adsorbability with respect to the cell culture surface of the support, or (2-iii) an amino acid sequence which is formed by the addition, substitution, or deletion of 1 to 30 amino acids in the amino acid sequence represented by SEQ ID NO: 2 and exhibits adsorbability with respect to the cell culture surface of the support.

Abstract translation: 多能干细胞的培养方法包括通过使用其中2-巯基乙醇的浓度等于或小于10μM的培养基，在多肽组成的培养基中培养多能干细胞，所述多肽由40〜 450个氨基酸残基，其中多肽包括（1）包含至少一个选自由CSYYQSC（SEQ ID NO：1）表示的氨基酸序列的氨基酸序列的氨基酸序列的第一结构域和由 RGD和（2）包含（2-i）由PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN（SEQ ID NO：2）表示的氨基酸序列的第二结构域，（2-ii）具有等于或高于 50％与SEQ ID NO：2表示的氨基酸序列相比，表现出对载体的细胞培养表面的吸附性，或（2-iii）通过添加，取代或取代形成的氨基酸序列 在SEQ ID NO：2所示的氨基酸序列中为1〜30个氨基酸，并且相对于载体的细胞培养物表面具有吸附性。

公开(公告)号：US20240233867A1

公开(公告)日：2024-07-11

申请号：US18412092

申请日：2024-01-12

Applicant: FUJIFILM Corporation

Inventor： Kentaro NAKAMURA ,  Yuichi YOSHINO ,  Rie HANDO

IPC: G16B25/10 ,  A61K35/28 ,  C12N5/0775

CPC classification number: G16B25/10 ,  A61K35/28 ,  C12N5/0662

Abstract: An object of the present invention is to provide such a quality management method for a cell that enables the sorting-out of a cell having drug efficacy, and a method of producing a cell using the quality management method. According to the present invention, there is provided a method that is a quality management method for a specific cell, where the method includes evaluating a correlation relationship between an expression pattern of a gene group obtained by measuring an expression state of a related gene group that is characteristic of the specific cell and an expression pattern that serves as a reference and determining the cell to be usable in a case where the correlation relationship is equal to or higher than a certain level.

公开(公告)号：US20160244728A1

公开(公告)日：2016-08-25

申请号：US15065217

申请日：2016-03-09

Applicant: FUJIFILM Corporation

Inventor： Keita HAGIYA ,  Yuta MURAKAMI ,  Yuichi YOSHINO ,  Sanae NOMIYAMA ,  Rie HANDO ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2500/38 ,  C12N2510/00 ,  C12N2533/50 ,  C12N2533/52

Abstract: A culture method for pluripotent stem cells includes obtaining a polypeptide-coated culture surface by applying a polypeptide to a cell culture surface of a support, and culturing pluripotent stem cells by seeding the pluripotent stem cells onto the polypeptide-coated culture surface by using a medium in which the content of an ascorbic acid derivative is equal to or greater than 1.5 mmol/L (mM), in which the polypeptide is (a) a polypeptide having an amino acid sequence represented by SEQ ID NO: 1, (b) a polypeptide having an amino acid sequence, which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID NO: 1, and having culture performance for pluripotent stem cells, or (c) a polypeptide having an amino acid sequence, which is formed by the deletion, substitution, or addition of one amino acid or several amino acids in SEQ ID NO: 1, and having culture performance for pluripotent stem cells.

Abstract translation: 多能干细胞的培养方法包括通过将多肽施用于载体的细胞培养物表面获得多肽包被的培养物表面，并通过使用培养基将多能干细胞接种到多肽包被的培养物表面上来培养多能干细胞 其中抗坏血酸衍生物的含量等于或大于1.5mmol / L（mM），其中多肽是（a）具有SEQ ID NO：1所示氨基酸序列的多肽，（b）a 具有氨基酸序列的多肽，其与SEQ ID NO：1所示的氨基酸序列具有等于或高于80％的同一性，并具有多能干细胞的培养性能，或（c）具有氨基酸的多肽 序列，其通过SEQ ID NO：1中的一个氨基酸或几个氨基酸的缺失，取代或添加而形成，并具有多能干细胞的培养性能。