公开(公告)号：US20160272945A1

公开(公告)日：2016-09-22

申请号：US15139497

申请日：2016-04-27

Applicant: FUJIFILM Corporation

Inventor： Keita HAGIYA ,  Sanae MORIOKA ,  Yuta MURAKAMI ,  Rie HANDO ,  Kouo SUZUKI ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/074 ,  C07K14/78

CPC classification number: C12N5/0696 ,  C07K14/78 ,  C12N5/0018 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/998

Abstract: A polypeptide composition induces a pluripotent stem cell culturing property, particularly, an excellent cell growth ability. The polypeptide composition contains a predetermined polypeptide including an amino acid sequence of human vitronectin or an amino acid sequence of a predetermined first region derived from human vitronectin. The polypeptide composition includes a multimeric polypeptide, which is composed of two or more monomers held together by intermolecular cross-linking via cysteine residues included in the first region, in an amount equal to or less than 20% by mass of a total mass of polypeptides contained in the composition. A culture method for pluripotent stem cells includes culturing pluripotent stem cells in the presence of the polypeptide composition. Also provided is a culture vessel including a support which has a cell culture surface and the polypeptide contained in the polypeptide composition disposed on the cell culture surface of the support.

Abstract translation: 多肽组合物诱导多能干细胞培养特性，特别是优良的细胞生长能力。 多肽组合物含有包含人玻连蛋白的氨基酸序列或来自人玻连蛋白的预定第一区的氨基酸序列的预定多肽。 多肽组合物包括多聚体多肽，其由两个或更多个通过分子间交联通过包含在第一区域中的半胱氨酸残基保持在一起的单体组成，其量等于或小于20质量％ 包含在组合物中。 多能干细胞的培养方法包括在多肽组合物存在下培养多能干细胞。 还提供了一种培养容器，其包括具有细胞培养表面的载体和多肽组合中所含的多肽，其设置在载体的细胞培养表面上。

公开(公告)号：US20200032183A1

公开(公告)日：2020-01-30

申请号：US16573126

申请日：2019-09-17

Applicant: FUJIFILM Corporation ,  KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION

Inventor： Kentaro NAKAMURA ,  Rie HANDO ,  Satoshi GOJO ,  Daisuke KAMI

IPC: C12M3/00 ,  C12M1/34 ,  C12N5/00 ,  C07K14/78

Abstract: An object of the present invention is to provide a method of manufacturing a large number of uniform cell structures for a short period of time. According to the present invention, provided is a method of manufacturing a cell structure, including: a step of adding a mixture of biocompatible macromolecular blocks, cells, and a liquid medium to a first culture container having a culture surface on which the plurality of recessed portions are formed and a side wall part standing on an outer periphery of the culture surface such that a liquid surface of the mixture is over the culture surface; a step of allowing a first culture container to stand and forming a cell structure in the recessed portion; and a step of stirring and culturing a content of the first culture container in a second culture container including stirring means.

公开(公告)号：US20160251613A1

公开(公告)日：2016-09-01

申请号：US15064619

申请日：2016-03-09

Applicant: FUJIFILM CORPORATION

Inventor： Yuta MURAKAMI ,  Sanae NOMIYAMA ,  Keita HAGIYA ,  Yuichi YOSHINO ,  Rie HANDO ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/00 ,  C12N5/074

CPC classification number: C12N5/0068 ,  C07K7/06 ,  C07K14/00 ,  C07K14/78 ,  C12N5/0696 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2533/30 ,  C12N2533/50

Abstract: A culture method for pluripotent stem cells includes culturing pluripotent stem cells on a cell culture surface of a support by using a medium in which the concentration of 2-mercaptoethano is equal to or less than 10 μM in the presence of a polypeptide consisting of 40 to 450 amino acid residues, in which the polypeptide includes (1) a first domain including at least one amino acid sequence selected from the group consisting of an amino acid sequence represented by CSYYQSC (SEQ ID NO: 1) and an amino acid sequence represented by RGD and (2) a second domain including (2-i) an amino acid sequence which is represented by PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN (SEQ ID NO: 2), (2-ii) an amino acid sequence which shares sequence identity of equal to or higher than 50% with the amino acid sequence represented by SEQ ID NO: 2 and exhibits adsorbability with respect to the cell culture surface of the support, or (2-iii) an amino acid sequence which is formed by the addition, substitution, or deletion of 1 to 30 amino acids in the amino acid sequence represented by SEQ ID NO: 2 and exhibits adsorbability with respect to the cell culture surface of the support.

Abstract translation: 多能干细胞的培养方法包括通过使用其中2-巯基乙醇的浓度等于或小于10μM的培养基，在多肽组成的培养基中培养多能干细胞，所述多肽由40〜 450个氨基酸残基，其中多肽包括（1）包含至少一个选自由CSYYQSC（SEQ ID NO：1）表示的氨基酸序列的氨基酸序列的氨基酸序列的第一结构域和由 RGD和（2）包含（2-i）由PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN（SEQ ID NO：2）表示的氨基酸序列的第二结构域，（2-ii）具有等于或高于 50％与SEQ ID NO：2表示的氨基酸序列相比，表现出对载体的细胞培养表面的吸附性，或（2-iii）通过添加，取代或取代形成的氨基酸序列 在SEQ ID NO：2所示的氨基酸序列中为1〜30个氨基酸，并且相对于载体的细胞培养物表面具有吸附性。

公开(公告)号：US20240233867A1

公开(公告)日：2024-07-11

申请号：US18412092

申请日：2024-01-12

Applicant: FUJIFILM Corporation

Inventor： Kentaro NAKAMURA ,  Yuichi YOSHINO ,  Rie HANDO

IPC: G16B25/10 ,  A61K35/28 ,  C12N5/0775

CPC classification number: G16B25/10 ,  A61K35/28 ,  C12N5/0662

Abstract: An object of the present invention is to provide such a quality management method for a cell that enables the sorting-out of a cell having drug efficacy, and a method of producing a cell using the quality management method. According to the present invention, there is provided a method that is a quality management method for a specific cell, where the method includes evaluating a correlation relationship between an expression pattern of a gene group obtained by measuring an expression state of a related gene group that is characteristic of the specific cell and an expression pattern that serves as a reference and determining the cell to be usable in a case where the correlation relationship is equal to or higher than a certain level.

公开(公告)号：US20160244728A1

公开(公告)日：2016-08-25

申请号：US15065217

申请日：2016-03-09

Applicant: FUJIFILM Corporation

Inventor： Keita HAGIYA ,  Yuta MURAKAMI ,  Yuichi YOSHINO ,  Sanae NOMIYAMA ,  Rie HANDO ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N2500/38 ,  C12N2510/00 ,  C12N2533/50 ,  C12N2533/52

Abstract: A culture method for pluripotent stem cells includes obtaining a polypeptide-coated culture surface by applying a polypeptide to a cell culture surface of a support, and culturing pluripotent stem cells by seeding the pluripotent stem cells onto the polypeptide-coated culture surface by using a medium in which the content of an ascorbic acid derivative is equal to or greater than 1.5 mmol/L (mM), in which the polypeptide is (a) a polypeptide having an amino acid sequence represented by SEQ ID NO: 1, (b) a polypeptide having an amino acid sequence, which shares identity of equal to or higher than 80% with the amino acid sequence represented by SEQ ID NO: 1, and having culture performance for pluripotent stem cells, or (c) a polypeptide having an amino acid sequence, which is formed by the deletion, substitution, or addition of one amino acid or several amino acids in SEQ ID NO: 1, and having culture performance for pluripotent stem cells.

Abstract translation: 多能干细胞的培养方法包括通过将多肽施用于载体的细胞培养物表面获得多肽包被的培养物表面，并通过使用培养基将多能干细胞接种到多肽包被的培养物表面上来培养多能干细胞 其中抗坏血酸衍生物的含量等于或大于1.5mmol / L（mM），其中多肽是（a）具有SEQ ID NO：1所示氨基酸序列的多肽，（b）a 具有氨基酸序列的多肽，其与SEQ ID NO：1所示的氨基酸序列具有等于或高于80％的同一性，并具有多能干细胞的培养性能，或（c）具有氨基酸的多肽 序列，其通过SEQ ID NO：1中的一个氨基酸或几个氨基酸的缺失，取代或添加而形成，并具有多能干细胞的培养性能。