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公开(公告)号:US20210277381A1
公开(公告)日:2021-09-09
申请号:US17323868
申请日:2021-05-18
摘要: Methods and systems for processing material in a host fluid use an acoustophoresis device. These methods and systems can deflect material (e.g., a second fluid, cells, beads or other particles, exosomes, viruses, oil droplets) in host fluid streams at high flow rates.
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公开(公告)号:US20200308533A1
公开(公告)日:2020-10-01
申请号:US16880787
申请日:2020-05-21
发明人: Bart Lipkens , Erik Miller , Benjamin Ross-Johnsrud , Walter M. Presz, JR. , Kedar Chitale , Thomas J. Kennedy, III , Lauryn Winiarski
摘要: Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.
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公开(公告)号:US20190011407A9
公开(公告)日:2019-01-10
申请号:US15963809
申请日:2018-04-26
发明人: Bart Lipkens , Rui Tostoes , Kedar C. Chitale , Benjamin Ross-Johnsrud , Walter M. Presz, JR. , Jack Saloio
IPC分类号: G01N30/76 , G01N30/46 , G01N30/72 , G01N33/543
摘要: Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.
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公开(公告)号:US20170217794A1
公开(公告)日:2017-08-03
申请号:US15490878
申请日:2017-04-18
发明人: Bart Lipkens , Walter M. Presz, JR. , Kedar Chitale , Brian McCarthy , Benjamin Ross-Johnsrud , Thomas J. Kennedy, III , Dane Mealey , Brian Dutra , David Sokolowski
CPC分类号: C02F1/36 , B01D17/04 , B01D17/041 , B01D17/044 , B01D19/0078 , B01D21/28 , B06B1/0622 , B06B1/0644 , C02F1/40 , C12M35/04 , C12M47/02 , C12N1/02 , C12N13/00 , G10K11/28 , H01L41/0913
摘要: Separation of particles or droplets from a host fluid may be achieved using a transducer and/or reflector that is a thin, non-planar structure. The thin non-planar structure improves operation of an acoustic standing wave generated by an acoustic transducer. The structure may operate as a pressure release boundary and may be constructed as plastic film.
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公开(公告)号:US20170175073A1
公开(公告)日:2017-06-22
申请号:US15453582
申请日:2017-03-08
发明人: Bart Lipkens , Louis Masi , Stanley Kowalski, III , Walter M. Presz, JR. , Jason Dionne , Brian Dutra , Ari Mercado , Thomas J. Kennedy, III , Arthur Martin , John Rozembersky
IPC分类号: C12M1/00
CPC分类号: C12M47/10 , B01D17/04 , B01D17/06 , B01D21/283 , B01J19/10 , B01J19/18 , B01J2219/00094 , B06B1/0644 , C12M23/14 , C12M23/22 , C12M27/02 , C12M29/10 , C12M29/18 , C12M33/08 , C12M33/14 , C12M35/04 , C12M47/02
摘要: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.
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公开(公告)号:US20150274550A1
公开(公告)日:2015-10-01
申请号:US14711627
申请日:2015-05-13
发明人: Bart Lipkens , Jason Dionne , Ari Mercado , Brian Dutra , Walter M. Presz, JR. , Thomas J. Kennedy, III , Louis Masi
CPC分类号: C02F1/36 , B01D17/045 , B01D17/12 , B01D21/283 , B01D43/00 , B06B1/0644 , C02F1/40 , C02F2103/007 , C02F2103/10
摘要: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.
摘要翻译: 使用声驻波在具有声流装置的流体流动方案中将组分与多组分流体(例如来自油 - 水混合物的油)分离。 例如,流动方案和装置允许在油聚结,聚集并且变得比水更浮力时捕获油。
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公开(公告)号:US20140154795A1
公开(公告)日:2014-06-05
申请号:US14175766
申请日:2014-02-07
发明人: Bart Lipkens , Louis Masi , Stanley Kowalski, III , Walter M. Presz, JR. , Jason Dionne , Brian Dutra , Ari Mercado , Thomas J. Kennedy, III , Arthur Martin
IPC分类号: C12M1/12
CPC分类号: C12M25/00 , B01D17/04 , B01D17/06 , B01D21/283 , B06B1/0644 , C12M29/10 , C12M33/08 , C12M35/04 , C12M47/02 , H01L41/0973
摘要: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.
摘要翻译: 灌注生物反应器包括至少一个可声学地产生多维驻波的超声换能器。 驻波可用于将细胞保留在生物反应器中,并且也可用于从生物反应器中生产的废物中脱水或进一步收获产品。
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公开(公告)号:US20190144843A1
公开(公告)日:2019-05-16
申请号:US16124184
申请日:2018-09-06
发明人: Bart Lipkens , Walter M. Presz, JR. , Brian Dutra , Jason Dionne , Goutam Ghoshal , Kedar C. Chitale
摘要: Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.
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公开(公告)号:US20180362918A1
公开(公告)日:2018-12-20
申请号:US16010296
申请日:2018-06-15
发明人: Bart Lipkens , Rui Tostoes , Brian Dutra , Rudolf Gilmanshin , Jason Dionne , Walter M. Presz, JR. , Benjamin Ross-Johnsrud , Goutam Ghoshal , Kedar Chitale
摘要: A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.
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公开(公告)号:US20180015392A1
公开(公告)日:2018-01-18
申请号:US15690263
申请日:2017-08-29
发明人: Bart Lipkens , Walter M. Presz, JR. , Jeffrey King , Jason Barnes , Dane Mealey , Brian McCarthy , Ben Ross-Johnsrud , Kedar Chitale
摘要: Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume.
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