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    Acoustic separation of cellular supporting materials from cultured cells
    4.
    发明授权
    Acoustic separation of cellular supporting materials from cultured cells 有权

    公开(公告)号：US09663756B1

    公开(公告)日：2017-05-30

    申请号：US15053359

    申请日：2016-02-25

    Applicant: FloDesign Sonics, Inc.

    Inventor： Bart Lipkens ,  Rudolf Gilmanshin ,  Ben Ross-Johnsrud ,  Kedar Chitale

    IPC: C12N5/00 ,  C12M1/00 ,  C12M1/26 ,  C12N13/00

    CPC classification number: C12M47/04 ,  C12M33/00 ,  C12M33/08 ,  C12M47/02 ,  C12N5/0062 ,  C12N13/00 ,  C12N2527/00

    Abstract: Methods, systems, and apparatus for acoustic separation of cellular supporting materials such as microcarriers or microbubbles from cell culture are provided. In one aspect, a method includes flowing a fluid containing the cellular supporting material and the cells through a flow chamber; driving at least one acoustic transducer to launch an acoustic wave from the acoustic transducer positioned on a first wall of the flow chamber to a reflector positioned on a second wall of the flow chamber to create, in the flow chamber, a multi-dimensional field that includes first spatial locales where acoustic pressure amplitude is elevated from a baseline level when the acoustic transducer is turned off, and second spatial locales where acoustic pressure amplitude is substantially identical to the baseline level when the acoustic transducer is turned off, wherein the first wall is opposite to the second wall; preferentially trapping, and gravity separating, the cellular supporting material or the cells at the first or second spatial locales inside the flow chamber; and collecting cells separated from the cellular supporting material using a first collection duct coupled to the flow chamber.

    DEVICE FOR MANIPULATING BIOLOGICAL CELLS USING A VIBRATING SUPPORT
    7.
    发明申请
    DEVICE FOR MANIPULATING BIOLOGICAL CELLS USING A VIBRATING SUPPORT 审中-公开
    使用振动支持来控制生物细胞的装置

    公开(公告)号：US20160244715A1

    公开(公告)日：2016-08-25

    申请号：US15046803

    申请日：2016-02-18

    Applicant: Commissariat a L'Energie Atomique et aux Energies Alternatives

    Inventor： Fabrice CASSET ,  Arnaud MILLET

    IPC: C12M1/00 ,  C12M1/26 ,  C12M3/06

    CPC classification number: C12M47/04 ,  C12M23/16 ,  C12M23/20 ,  C12M25/02 ,  C12M33/08

    Abstract: Device for sorting living cells, comprising at least one support including a surface having adherence properties with respect to said type of living cells, actuators capable of making said surface vibrate at at least one given frequency and a controller for controlling the actuators such that the surface vibrates at a frequency causing the detachment of at least one type of living cells.

    Abstract translation: 用于分选活细胞的装置，包括至少一个支撑件，其包括相对于所述类型的活细胞具有粘附性质的表面，能够使所述表面以至少一个给定频率振动的致动器和用于控制致动器的控制器， 以导致至少一种类型的活细胞分离的频率振动。

    CELL DETACHMENT DEVICE
    8.
    发明申请
    CELL DETACHMENT DEVICE 有权
    细胞分离装置

    公开(公告)号：US20150315537A1

    公开(公告)日：2015-11-05

    申请号：US14650053

    申请日：2013-12-06

    Applicant: KAWASAKI JUKOGYO KABUSHIKI KAISHA

    Inventor： Keizo WATAKABE ,  Junji IIZAKA ,  Hitoshi HASUNUMA ,  Katsumi NAKASHIMA

    IPC: C12M1/26

    CPC classification number: C12M33/08

    Abstract: A cell detachment device includes: a base; a vessel holder, to which a culture vessel is to be fitted; a guide mechanism configured to guide reciprocating movement of the vessel holder; a collided member configured to collide with the vessel holder when the vessel holder is moving; an urging member configured to urge the culture vessel toward the collided member; and a power imparting mechanism configured to impart, to the vessel holder, power for causing the vessel holder to move.

    Abstract translation: 细胞分离装置包括：基底; 将要安装培养容器的容器支架; 引导机构，其构造成引导所述容器保持器的往复运动; 碰撞构件，构造成当所述容器保持器移动时与所述容器保持器碰撞; 推动构件，其构造成将所述培养容器推向所述碰撞构件; 以及动力施加机构，其构造成将容器保持器移动的动力赋予所述容器保持器。

    METHODS FOR INOCULATING CULTURE MEDIA ON PETRI DISHES BY MEANS OF VIBRATION FREQUENCIES
    9.
    发明申请
    METHODS FOR INOCULATING CULTURE MEDIA ON PETRI DISHES BY MEANS OF VIBRATION FREQUENCIES 有权
    通过振动频率对培养基培养培养基的方法

    公开(公告)号：US20140220616A1

    公开(公告)日：2014-08-07

    申请号：US14238673

    申请日：2012-08-30

    Applicant: Bruno Colin ,  Marie-Pierre Montet

    Inventor： Bruno Colin ,  Marie-Pierre Montet

    IPC: C12Q1/24 ,  C12M1/22

    CPC classification number: C12Q1/24 ,  C12M23/10 ,  C12M23/38 ,  C12M33/08

    Abstract: The present invention relates to a method for inoculating at least one agar culture medium, contained in a Petri dish, with at least one sample likely to contain microorganisms. The method includes the steps of: providing at least one agar culture medium contained in a Petri dish; providing the sample likely to contain microorganisms in liquid form; performing at least one deposition of the liquid sample onto the lid of the Petri dish such that the liquid sample can be transferred from the lid to the agar culture medium; and spraying the liquid sample, initially deposited onto the cover, onto the agar culture medium by vibrating the Petri dish.

    Abstract translation: 本发明涉及用至少一种可能含有微生物的样品接种培养皿中含有的至少一种琼脂培养基的方法。 该方法包括以下步骤：提供培养皿中所含的至少一种琼脂培养基; 提供样品可能含有液体形式的微生物; 将液体样品的至少一次沉积物执行到培养皿的盖上，使得液体样品可以从盖子转移到琼脂培养基中; 并通过振动培养皿将初始沉积到盖上的液体样品喷洒到琼脂培养基上。

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