公开(公告)号：US5268277A

公开(公告)日：1993-12-07

申请号：US834486

申请日：1992-02-12

申请人： Guido Grandi ,  Elisabetta Franchi ,  Federico Maisano ,  Silvia A. Testori

发明人： Guido Grandi ,  Elisabetta Franchi ,  Federico Maisano ,  Silvia A. Testori

IPC分类号： A61K38/00 ,  C07K14/61 ,  C12N9/56 ,  C12N15/75 ,  C12P21/02 ,  C12N15/00

CPC分类号： C12N9/54 ,  C07K14/61 ,  C12N15/75 ,  A61K38/00 ,  C07K2319/036 ,  C07K2319/75

摘要： A hybrid plasmid which is capable of inducing the expression and the secretion of human growth hormone (hGH) in its natural form and a method for the preparation of hGH which comprises the culture in a suitable environment of Bacillus subtilis cells transformed by the hybrid plasmid, are described.The hGH thus obtained has the correct amino acid sequence and is particularly useful for treatment of man.

摘要翻译： 能够以其天然形式诱导人生长激素（hGH）的表达和分泌的杂交质粒和制备hGH的方法，其包括在杂交质粒转化的枯草芽孢杆菌细胞的合适环境中培养， 被描述。 所得hGH具有正确的氨基酸序列，特别适用于人的治疗。

公开(公告)号：US5047333A

公开(公告)日：1991-09-10

申请号：US288358

申请日：1988-12-22

申请人： Guido Grandi ,  Elisabetta Franchi ,  Federico Maisano ,  Silvia Astrua Testori

发明人： Guido Grandi ,  Elisabetta Franchi ,  Federico Maisano ,  Silvia Astrua Testori

IPC分类号： C12P21/02 ,  A61K38/27 ,  A61P3/04 ,  A61P17/00 ,  A61P43/00 ,  C07K1/20 ,  C07K14/00 ,  C07K14/575 ,  C07K14/61 ,  C12N15/18 ,  C12N15/62 ,  C12N15/75 ,  C12R1/07

CPC分类号： C12N15/75 ,  C07K14/61 ,  C12N15/62 ,  C07K2319/50

摘要： An improved method is described for the preparation of natural human growth hormone (hGH) in pure form, which comprises the preparation of a precursor of hGH by means of the culture of Bacillus subtilis cells transformed by a hybrid plasmid, the separation of the precursor from the total proteins, the digestion of the precursor with the enzyme Factor Xa and finally the purification of the natural hGH thus obtained from the enzymatic hydrolysis mixture.

公开(公告)号：US5334531A

公开(公告)日：1994-08-02

申请号：US837659

申请日：1992-02-14

申请人： Marina Del Bue ,  Paola Cosmina ,  Elisabetta Franchi ,  Guido Grandi

发明人： Marina Del Bue ,  Paola Cosmina ,  Elisabetta Franchi ,  Guido Grandi

IPC分类号： C12N15/09 ,  A61K38/00 ,  C07K14/61 ,  C12N1/21 ,  C12N15/18 ,  C12N15/70 ,  C12N15/75 ,  C12P21/02 ,  C12R1/07 ,  C12R1/125 ,  G01N33/50

CPC分类号： C12N15/70 ,  C07K14/61 ,  C12N15/75 ,  A61K38/00 ,  Y10S435/832 ,  Y10S435/839

摘要： The description relates to a plasmid vector expression in Bacillus and used for cloning the structural gene which codes for the human growth hormone, a recombinant DNA molecule comprising plasmid vector and the structural gene of the human growth hormone and a method of preparing the hormone comprising inserting the recombinant DNA molecule into strains of Bacillus and cultivating the resulting transformed strains in a suitable culture environment and recovering the thus-synthesized hormone from the cells.

摘要翻译： 描述涉及在芽孢杆菌中的质粒载体表达，用于克隆编码人生长激素的结构基因，包含质粒载体和人生长激素的结构基因的重组DNA分子和制备激素的方法，包括插入 将重组DNA分子转化到芽孢杆菌菌株中，并在合适的培养环境中培养所得的转化菌株，并从细胞中回收合成的激素。

公开(公告)号：US09206399B2

公开(公告)日：2015-12-08

申请号：US11666786

申请日：2005-10-28

申请人： Jeannette Adu-Bobie ,  Mariagrazia Pizza ,  Nathalie Norais ,  Germano Ferrari ,  Guido Grandi

发明人： Jeannette Adu-Bobie ,  Mariagrazia Pizza ,  Nathalie Norais ,  Germano Ferrari ,  Guido Grandi

IPC分类号： A61K39/095 ,  C12N9/10 ,  A61K39/108

CPC分类号： C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K39/095 ,  C07K14/22 ,  C12N9/1051 ,  Y02A50/474

摘要： Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titres than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity of MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.

摘要翻译： 脑膜炎球菌mltA同系物的敲除使细菌自发释放富含免疫原性外膜蛋白的囊泡，并且能够以比正常生产过程制备的OMV更高的杀菌滴度引发交叉保护性抗体应答。 因此，本发明提供了具有其mltA基因的敲除突变的细菌。 本发明还提供了一种细菌，其中所述细菌：（i）具有包含肽聚糖的细胞壁; 和（ii）不表达具有MltA蛋白的裂解转糖苷酶活性的蛋白质。 本发明还提供包含囊泡的组合物，其在本发明的细菌培养期间释放到培养基中。

公开(公告)号：US09067987B2

公开(公告)日：2015-06-30

申请号：US13405213

申请日：2012-02-24

申请人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Marirosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

发明人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Marirosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

IPC分类号： C07K14/22 ,  A61K39/095 ,  A61K39/00 ,  A61K48/00

CPC分类号： A61K39/095 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/55505 ,  C07K14/22

摘要： This invention provides, among other things, proteins, polypeptides, and fragments thereof, derived from the bacteria Neisseria meningitidis B. Also provided are nucleic acids encoding for such proteins, polypeptides, and/or fragments, as well as nucleic acids complementary thereto e.g., antisense nucleic acids). Additionally, this invention provides antibodies which bind to the proteins, polypeptides, and/or fragments. This invention further provides expression vectors useful for making the proteins, polypeptides, and/or fragments, as well as host cells transformed with such vectors. This invention also provides compositions of the proteins, polypeptides, fragments, and/or nucleic acids, for use as vaccines, diagnostic reagents, immunogenic compositions, and the like. Methods of making the compositions and methods of treatment with the compositions are also provided. This invention also provides methods of detecting the proteins, polypeptides, fragments, and/or nucleic acids.

摘要翻译： 本发明尤其提供衍生自脑膜炎奈瑟氏球菌B细菌的蛋白质，多肽和其片段。还提供编码这种蛋白质，多肽和/或片段的核酸，以及与其互补的核酸，例如， 反义核酸）。 另外，本发明提供了结合蛋白质，多肽和/或片段的抗体。 本发明进一步提供了可用于制备蛋白质，多肽和/或片段以及用这些载体转化的宿主细胞的表达载体。 本发明还提供用作疫苗，诊断试剂，免疫原性组合物等的蛋白质，多肽，片段和/或核酸的组合物。 还提供了用组合物制备组合物和处理方法的方法。 本发明还提供了检测蛋白质，多肽，片段和/或核酸的方法。

公开(公告)号：US08858957B2

公开(公告)日：2014-10-14

申请号：US13830774

申请日：2013-03-14

申请人： Immaculada Margarit Y Ros ,  Guido Grandi ,  Chiara Zingaretti

发明人： Immaculada Margarit Y Ros ,  Guido Grandi ,  Chiara Zingaretti

IPC分类号： A61K39/09 ,  A61K39/02 ,  A61K39/00 ,  A61K38/00 ,  C07K1/00 ,  C07K14/315 ,  C07K16/12 ,  C07K16/40

CPC分类号： C07K14/315 ,  A61K38/164 ,  A61K38/18 ,  A61K38/19 ,  A61K38/22 ,  A61K39/092 ,  A61K45/06 ,  A61K2039/522 ,  A61K2039/6037 ,  A61K2039/6043 ,  A61K2039/6068 ,  C07K14/22 ,  C07K14/235 ,  C07K14/285 ,  C07K14/33 ,  C07K14/475 ,  C07K14/52 ,  C07K14/575 ,  C07K16/1275 ,  C07K16/40 ,  C07K2319/00 ,  C07K2319/55 ,  Y02A50/466 ,  Y02A50/478 ,  Y10S530/825

摘要： The invention provides mutants of GAS57 (Spy0416) which are unable to cleave IL-8 and similar substrates but which still maintain the ability to induce protection against S. pyogenes. The invention also provides antibodies which specifically bind to GAS57 and which inhibit its ability to cleave IL-8 and similar substrates. The mutants are useful, inter alia, in vaccine compositions to induce protection against S. pyogenes. The antibodies are useful, e.g., as therapeutics for treating S. pyogenes infections.

摘要翻译： 本发明提供的GAS57突变体（Spy0416）不能切割IL-8和类似的底物，但仍保持对化脓性链球菌的保护的能力。 本发明还提供了特异性结合GAS57并且抑制其分裂IL-8和类似底物的能力的抗体。 突变体尤其可用于诱导保护成脓性葡萄球菌的疫苗组合物。 抗体是有用的，例如用作治疗化脓性链球菌感染的治疗剂。

公开(公告)号：US08481057B2

公开(公告)日：2013-07-09

申请号：US12086571

申请日：2006-12-19

申请人： Guido Grandi ,  Lehutova Livia

发明人： Guido Grandi ,  Giulio Ratti

IPC分类号： A61K39/118 ,  C07K14/295

CPC分类号： A61K39/118

摘要： The invention is in the field of immunology and vaccinology. In particular, it relates to antigens derived from Chlamydia trachomatis that are expressed on the cell surface and so are ideal for use in immunization as well as combinations of these antigens.

摘要翻译： 本发明在免疫学和疫苗学领域。 特别地，本发明涉及在细胞表面表达的来自沙眼衣原体的抗原，因此是用于免疫的理想用途以及这些抗原的组合。

公开(公告)号：US20120276129A1

公开(公告)日：2012-11-01

申请号：US13405213

申请日：2012-02-24

申请人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Mariarosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

发明人： Cesira Galeotti ,  Guido Grandi ,  Vega Masignani ,  Mariarosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

IPC分类号： A61K39/095 ,  A61P37/04 ,  C07K14/22

CPC分类号： A61K39/095 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/55505 ,  C07K14/22

摘要： This invention provides, among other things, proteins, polypeptides, and fragments thereof, derived from the bacteria Neisseria meningitidis B. Also provided are nucleic acids encoding for such proteins, polypeptides, and/or fragments, as well as nucleic acids complementary thereto e.g., antisense nucleic acids). Additionally, this invention provides antibodies which bind to the proteins, polypeptides, and/or fragments. This invention further provides expression vectors useful for making the proteins, polypeptides, and/or fragments, as well as host cells transformed with such vectors. This invention also provides compositions of the proteins, polypeptides, fragments, and/or nucleic acids, for use as vaccines, diagnostic reagents, immunogenic compositions, and the like. Methods of making the compositions and methods of treatment with the compositions are also provided. This invention also provides methods of detecting the proteins, polypeptides, fragments, and/or nucleic acids.

摘要翻译： 本发明尤其提供衍生自脑膜炎奈瑟氏球菌B细菌的蛋白质，多肽和其片段。还提供编码这种蛋白质，多肽和/或片段的核酸，以及与其互补的核酸，例如， 反义核酸）。 另外，本发明提供了结合蛋白质，多肽和/或片段的抗体。 本发明进一步提供了可用于制备蛋白质，多肽和/或片段以及用这些载体转化的宿主细胞的表达载体。 本发明还提供用作疫苗，诊断试剂，免疫原性组合物等的蛋白质，多肽，片段和/或核酸的组合物。 还提供了用组合物制备组合物和处理方法的方法。 本发明还提供了检测蛋白质，多肽，片段和/或核酸的方法。

公开(公告)号：US20120219578A1

公开(公告)日：2012-08-30

申请号：US13405162

申请日：2012-02-24

申请人： Cesira GALEOTTI ,  Guido Grandi ,  Vega Masignani ,  Mariarosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

发明人： Cesira GALEOTTI ,  Guido Grandi ,  Vega Masignani ,  Mariarosa Mora ,  Mariagrazia Pizza ,  Rino Rappuoli ,  Guilio Ratti ,  Vincenzo Scarlato ,  Maria Scarselli

IPC分类号： A61K39/095 ,  A61P31/04 ,  A61P37/04 ,  C07K14/22

CPC分类号： A61K39/095 ,  A61K39/00 ,  A61K48/00 ,  A61K2039/55505 ,  C07K14/22

摘要： This invention provides, among other things, proteins, polypeptides, and fragments thereof, derived from the bacteria Neisseria meningitidis B. Also provided are nucleic acids encoding for such proteins, polypeptides, and/or fragments, as well as nucleic acids complementary thereto e.g., antisense nucleic acids). Additionally, this invention provides antibodies which bind to the proteins, polypeptides, and/or fragments. This invention further provides expression vectors useful for making the proteins, polypeptides, and/or fragments, as well as host cells transformed with such vectors. This invention also provides compositions of the proteins, polypeptides, fragments, and/or nucleic acids, for use as vaccines, diagnostic reagents, immunogenic compositions, and the like. Methods of making the compositions and methods of treatment with the compositions are also provided. This invention also provides methods of detecting the proteins, polypeptides, fragments, and/or nucleic acids.

公开(公告)号：US20120207778A1

公开(公告)日：2012-08-16

申请号：US13398079

申请日：2012-02-16

申请人： John Telford ,  Vega Masignani ,  Immaculada Margarit Y Ros ,  Guido Grandi ,  Claire Fraser ,  Hervé Tettelin

发明人： John Telford ,  Vega Masignani ,  Immaculada Margarit Y Ros ,  Guido Grandi ,  Claire Fraser ,  Hervé Tettelin

IPC分类号： A61K39/09 ,  C07K16/12 ,  C07K7/08 ,  A61K38/16 ,  A61P31/04 ,  C07K7/06 ,  C07K14/315 ,  C07H21/04

CPC分类号： C07K14/315 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/53 ,  C07K2319/00 ,  Y02A50/464 ,  Y02A50/466 ,  Y02A90/22 ,  Y02A90/26

摘要： The invention provides proteins from group B streptococcus (Streptococcus agalactiae) and group A streptococcus (Streptococcus pyogenes), including amino acid sequences and the corresponding nucleotide sequences.

摘要翻译： 本发明提供来自B族链球菌（无乳链球菌）和A型链球菌（化脓链球菌）的蛋白质，包括氨基酸序列和相应的核苷酸序列。