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    Mesenchymal precursor cell
    2.
    发明授权
    Mesenchymal precursor cell 有权
    间充质前体细胞

    公开(公告)号：US07122178B1

    公开(公告)日：2006-10-17

    申请号：US10030411

    申请日：2000-07-07

    申请人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    发明人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    IPC分类号： A01N63/00 ,  A61K35/26 ,  C12N5/00 ,  C12N5/08

    CPC分类号： C12N5/0663 ,  A61K2035/124 ,  C12N2500/42 ,  C12N2501/39

    摘要： A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

    摘要翻译： 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i）对间质前体细胞特异性标志物的存在，ii）不存在对分化的间充质细胞特异性的标记，或iii）对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤，随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤，以及 VCAM-1的表达。

    Mesenchymal precursor cell
    3.
    发明申请
    Mesenchymal precursor cell 有权

    公开(公告)号：US20060008452A1

    公开(公告)日：2006-01-12

    申请号：US11178920

    申请日：2005-07-11

    申请人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    发明人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    IPC分类号： A61K48/00 ,  C12N5/08

    CPC分类号： C12N5/0663 ,  A61K2035/124 ,  C12N2500/42 ,  C12N2501/39

    摘要： A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

    PERIVASCULAR MESENCHYMAL PRECURSOR CELL INDUCED BLOOD VESSEL FORMATION
    4.
    发明申请
    PERIVASCULAR MESENCHYMAL PRECURSOR CELL INDUCED BLOOD VESSEL FORMATION 审中-公开
    血管内膜前列腺细胞诱导血管形成

    公开(公告)号：US20160317581A1

    公开(公告)日：2016-11-03

    申请号：US14871234

    申请日：2015-09-30

    申请人： Stan Gronthos ,  Andrew Zannettino

    发明人： Stan Gronthos ,  Andrew Zannettino

    IPC分类号： A61K35/28 ,  C12N5/0775 ,  A61K9/00

    摘要： Mesenchymal precursors cells have been isolated from perivascular niches from a range of tissues utilizing a perivascular marker. A new mesenchymal precursor cell phenotype is described characterized by the presence of the perivascular marker 3G5, and preferably also alpha smooth muscle actin together with early developmental markers such as STRO-1 and CD146/MUC18. The perivascular mesenchymal precursor cell is shown to induce neovascularisation and improvement in cardiac function. Suitable administration of preparations of the mesenchymal precursor cells are useful for treatment of cardiovascular diseases, cerebrovascular diseases and peripheral vascular diseases.

    摘要翻译： 间充质前体细胞已经使用血管周围标志物从一系列组织的血管周围位置中分离出来。 描述了新的间充质前体细胞表型，其特征在于存在血管周围标志物3G5，优选还包括α平滑肌肌动蛋白以及早期发育标志物如STRO-1和CD146 / MUC18。 显示血管周围间充质前体细胞诱导心脏功能的新生血管形成和改善。 间充质前体细胞的制剂的合适施用可用于治疗心血管疾病，脑血管疾病和外周血管疾病。

    PERIVASCULAR MESENCHYMAL PRECURSOR CELLS
    5.
    发明申请
    PERIVASCULAR MESENCHYMAL PRECURSOR CELLS 审中-公开
    周围蛋白前体细胞

    公开(公告)号：US20120082649A1

    公开(公告)日：2012-04-05

    申请号：US13098892

    申请日：2011-05-02

    申请人： Stan Gronthos ,  Andrew Zannettino ,  Songtao Shi

    发明人： Stan Gronthos ,  Andrew Zannettino ,  Songtao Shi

    IPC分类号： A61K35/12 ,  C12N5/077

    摘要： Mesenchymal precursors cells have been isolated from perivascular niches from a range of tissues utilizing a perivascular marker. A new mesenchymal precursor cell phenotype is described characterized by the presence of the perivascular marker 3G5, and preferably also alpha smooth muscle actin together with early developmental markers such as MUC 18, VCAM-1 and STRO-1bri. The perivascular mesenchymal precursor cell is multipotential and is shown to form, vascular tissue, as well as bone marrow dentin and pulp. A method of enriching using cell sorting based on these markers is also described.

    摘要翻译： 间充质前体细胞已经使用血管周围标志物从一系列组织的血管周围位置中分离出来。 描述了新的间充质前体细胞表型，其特征在于存在血管周围标志物3G5，并且优选还包括α平滑肌肌动蛋白以及早期发育标志物如MUC18，VCAM-1和STRO-1bri。 血管周围间质前体细胞是多能的，并且显示出形成血管组织，以及骨髓牙本质和纸浆。 还描述了使用基于这些标记的细胞分选进行富集的方法。

    Mesenchymal precursor cell
    6.
    发明申请
    Mesenchymal precursor cell 有权
    间充质前体细胞

    公开(公告)号：US20050281790A1

    公开(公告)日：2005-12-22

    申请号：US11169875

    申请日：2005-06-29

    申请人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    发明人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    IPC分类号： A61K35/12 ,  C12N5/0775 ,  A61K45/00 ,  C12N5/08

    CPC分类号： C12N5/0663 ,  A61K2035/124 ,  C12N2500/42 ,  C12N2501/39

    摘要： A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

    摘要翻译： 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i）对间质前体细胞特异性标志物的存在，ii）不存在对分化的间充质细胞特异性的标记，或iii）对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤，随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤，以及 VCAM-1的表达。

    Mesenchymal precursor cell
    8.
    发明授权
    Mesenchymal precursor cell 有权
    间充质前体细胞

    公开(公告)号：US08158118B2

    公开(公告)日：2012-04-17

    申请号：US11169875

    申请日：2005-06-29

    申请人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    发明人： Paul Simmons ,  Andrew Zannettino ,  Stan Gronthos

    IPC分类号： A01N63/00 ,  A61K35/26 ,  C12N5/08 ,  C12N5/00

    CPC分类号： C12N5/0663 ,  A61K2035/124 ,  C12N2500/42 ,  C12N2501/39

    摘要： A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two color FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

    摘要翻译： 富含间充质前体的方法包括基于至少两种标记物富集细胞的步骤。 标记可能是i）对间质前体细胞特异性标志物的存在，ii）不存在对分化的间充质细胞特异性的标记，或iii）对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤，随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤，以及 VCAM-1的表达。

    Mesenchymal precursor cell
    9.
    发明授权
    Mesenchymal precursor cell 有权
    间充质前体细胞

    公开(公告)号：US08546138B2

    公开(公告)日：2013-10-01

    申请号：US13270863

    申请日：2011-10-11

    申请人： Stan Gronthos ,  Andrew Zannettino ,  Paul Simmons

    发明人： Stan Gronthos ,  Andrew Zannettino ,  Paul Simmons

    IPC分类号： C12N5/08 ,  C12N5/00 ,  C12N5/02

    CPC分类号： A61K35/28 ,  A61K47/48861 ,  A61K47/6923 ,  A61K48/00 ,  A61K2035/124 ,  C12N5/0663 ,  C12N2500/42 ,  C12N2501/39

    摘要： A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilising MACS recognising expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

    摘要翻译： 一种富含间充质前体细胞的方法，包括基于至少两种标记物富集细胞的步骤。 标记可能是i）对间质前体细胞特异性标志物的存在，ii）不存在对分化的间充质细胞特异性的标记，或iii）对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤，随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤，以及 VCAM-1的表达。

    MESENCHYMAL PRECURSOR CELL
    10.
    发明申请
    MESENCHYMAL PRECURSOR CELL 有权

    公开(公告)号：US20120276553A1

    公开(公告)日：2012-11-01

    申请号：US13270863

    申请日：2011-10-11

    申请人： Stan Gronthos ,  Andrew Zannettino ,  Paul Simmons

    发明人： Stan Gronthos ,  Andrew Zannettino ,  Paul Simmons

    IPC分类号： C12N5/0775 ,  G01N33/577 ,  C12N15/85 ,  G01N33/566

    CPC分类号： A61K35/28 ,  A61K47/48861 ,  A61K47/6923 ,  A61K48/00 ,  A61K2035/124 ,  C12N5/0663 ,  C12N2500/42 ,  C12N2501/39

    摘要： A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilising MACS recognising expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilising two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.

    摘要翻译： 一种富含间充质前体细胞的方法，包括基于至少两种标记物富集细胞的步骤。 标记可能是i）对间质前体细胞特异性标志物的存在，ii）不存在对分化的间充质细胞特异性的标记，或iii）对间充质前体细胞特异的标志物的表达水平。 该方法可以包括利用识别STRO-1 Mab抗原表达的MACS的第一固相分选步骤，随后使用两色FACS来筛选高水平STRO-1抗原表达的存在的第二分选步骤，以及 VCAM-1的表达。