公开(公告)号：US6066484A

公开(公告)日：2000-05-23

申请号：US912560

申请日：1997-08-18

申请人： Haruyo Hatanaka ,  Toshihiko Ashikari ,  Jun Ogawa ,  Sakayu Shimizu

发明人： Haruyo Hatanaka ,  Toshihiko Ashikari ,  Jun Ogawa ,  Sakayu Shimizu

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12C5/00 ,  C12C7/00 ,  C12N1/21 ,  C12N9/24 ,  C12N15/00 ,  C12N15/56 ,  C12R1/01 ,  C12R1/19 ,  C12N9/16 ,  C12C1/00

CPC分类号： C12Y302/02001 ,  C12C5/00 ,  C12C5/004 ,  C12C7/00 ,  C12N9/2497

摘要： Novel purine nucleosidase derived from Ochrobactrum anthropi microorganisms, a gene system coding therefor, uses therefor and particularly its use in the production of beer.

摘要翻译： 衍生自Ochrobactrum人参微生物的新型嘌呤核苷酸酶，其编码的基因系统用于其，特别是其用于生产啤酒。

公开(公告)号：US20080269329A1

公开(公告)日：2008-10-30

申请号：US11660859

申请日：2005-08-19

申请人： Shigeaki Fujikawa ,  Eiji Sakuradani ,  Jun Ogawa ,  Sakayu Shimizu

发明人： Shigeaki Fujikawa ,  Eiji Sakuradani ,  Jun Ogawa ,  Sakayu Shimizu

IPC分类号： C12P7/64 ,  A61K31/201 ,  C07C57/00 ,  A61K31/202

CPC分类号： A61K8/375 ,  A23D9/013 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12 ,  A23V2002/00 ,  A61K8/67 ,  A61K8/922 ,  A61K8/99 ,  A61Q19/00 ,  C11B1/10 ,  C11B3/12 ,  C12P7/6472 ,  A23V2250/182 ,  A23V2250/1882

摘要： In a process for the production of diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid, a process which is characterized in that a microbe being able to produce said fat/oil is incubated and, if desired, said fat/oil is collected.

摘要翻译： 在制备含二酰基甘油的脂肪/油的方法中，其中二酰基甘油与总中性脂质的比例大于20重量％，多不饱和脂肪酸是构成脂肪酸的方法，其特征在于将微生物 能够产生所述脂肪/油的温育，如果需要，收集所述脂肪/油。

公开(公告)号：US08273562B2

公开(公告)日：2012-09-25

申请号：US13344110

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov, legal representative ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C12Q1/68 ,  C12P21/06 ,  C12P21/04 ,  C07H21/04

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

公开(公告)号：US07927843B2

公开(公告)日：2011-04-19

申请号：US11878558

申请日：2007-07-25

申请人： Sakayu Shimizu ,  Jun Ogawa

发明人： Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12P19/02

CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphates or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphates or the enzymes derived from the microorganism.

摘要翻译： 本发明公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶但基本上不含磷酸盐的微生物本身存在下使甘油醛-3-磷酸和乙醛反应制备2-脱氧核糖-5-磷酸的方法， 微生物。 本发明还公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶和丙糖磷酸异构酶但基本上不含磷酸盐的微生物本身存在下使磷酸二羟丙酮和乙醛反应制备2-脱氧核糖-5-磷酸的方法，或者 源于微生物的酶。

公开(公告)号：US20070298468A1

公开(公告)日：2007-12-27

申请号：US11878558

申请日：2007-07-25

申请人： Sakayu Shimizu ,  Jun Ogawa

发明人： Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12P19/02

CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.

公开(公告)号：US09051590B2

公开(公告)日：2015-06-09

申请号：US13254512

申请日：2010-03-17

申请人： Misa Ochiai ,  Eiji Sakuradani ,  Sakayu Shimizu ,  Jun Ogawa

发明人： Misa Ochiai ,  Eiji Sakuradani ,  Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P21/06 ,  C12P21/04 ,  C12N15/00 ,  C12P7/64 ,  A23L1/30 ,  C12N9/00

CPC分类号： C12P7/6427 ,  A23L33/115 ,  A23V2002/00 ,  C12N9/93 ,  C12Y604/01002 ,  A23V2250/1882

摘要： The present invention provides a novel acetyl-CoA carboxylase.The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.

摘要翻译： 本发明提供了一种新的乙酰辅酶A羧化酶。 本发明的目的是通过本发明的SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的氨基酸序列来实现的。

公开(公告)号：US08367381B2

公开(公告)日：2013-02-05

申请号：US13344170

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi ,  Vitaly Grigorievich Paraskevov

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06 ,  C12P21/06 ,  C12P21/04 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/26 ,  C07H21/04

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

摘要翻译： 提供了一种来自苏云金芽孢杆菌的高活性L-异亮氨酸双加氧酶。 在L-异亮氨酸双加氧酶存在下，通过使L-异亮氨酸在水性溶剂中反应，并分离（2S，3R，4S） - 还提供了4-羟基-L-异亮氨酸。

公开(公告)号：US20120107883A1

公开(公告)日：2012-05-03

申请号：US13344170

申请日：2012-01-05

申请人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

发明人： Tomohiro Kodera ,  Sergey Vasilievich Smirnov ,  Natalia Nikolaevna Samsonova ,  Veronika Aleksandrovna Kotliarova ,  Natalia Yurievna Rushkevich ,  Yury Ivanovich Kozlov ,  Vitaly Grigorievich Paraskevov ,  Sakayu Shimizu ,  Jun Ogawa ,  Makoto Hibi

IPC分类号： C12P13/06

CPC分类号： C12N9/0069 ,  C12P13/06

摘要： A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.

公开(公告)号：US20070298467A1

公开(公告)日：2007-12-27

申请号：US11878553

申请日：2007-07-25

申请人： Sakayu Shimizu ,  Jun Ogawa

发明人： Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12P19/02

CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.

摘要翻译： 本发明公开了一种在含有2-脱氧核糖-5-磷酸醛缩酶但基本上不含磷酸酶的微生物本身存在下使甘油醛-3-磷酸和乙醛反应制备2-脱氧核糖-5-磷酸的方法， 微生物。 本发明还公开了在含有2-脱氧核糖-5-磷酸醛缩酶和丙糖磷酸异构酶但基本上不含磷酸酶的微生物本身的存在下使磷酸二羟丙酮和乙醛反应制备2-脱氧核糖-5-磷酸的方法，或者 源于微生物的酶。

公开(公告)号：US07270992B2

公开(公告)日：2007-09-18

申请号：US10652252

申请日：2003-09-02

申请人： Sakayu Shimizu ,  Jun Ogawa

发明人： Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12P19/02

CPC分类号： C12R1/22 ,  C12N9/88 ,  C12N9/90 ,  C12P19/02

摘要： The present invention discloses a method of preparing 2-deoxyribose 5-phosphate by reacting glyceraldehyde 3-phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase but substantially no phosphatase or the enzyme derived from the microorganism. The present invention also discloses a method of preparing 2-deoxyribose 5-phosphate by reacting dihydroxyacetone phosphate and acetaldehyde in the presence of either a microorganism itself which contains 2-deoxyribose-5-phosphate aldolase and triose-phosphate isomerase but substantially no phosphatase or the enzymes derived from the microorganism.