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公开(公告)号：US20140045701A1
公开(公告)日：2014-02-13
申请号：US13824129
申请日：2011-10-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.
摘要翻译： 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。
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公开(公告)号：US09399217B2
公开(公告)日：2016-07-26
申请号：US13481858
申请日：2012-05-27
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.
摘要翻译： 本发明的方面包括通过利用未标记的非可合并核苷酸或通过重新构建基于集落的测序反应来提高测序反应的准确性和读取长度的方法。 其他方面包括用于改进与珠粒相关的生物反应测量的系统和装置，其可以通过与所述珠粒相关联的抗衡离子的电流测量方法，由于存在与所述珠粒结合或相关联的反应物，其中用于产生和 测量所述电流可以在所述焊道的德拜长度内。 本发明的其它方面包括用于确定输入样品的浓度的方法，用于重复使用阵列的装置，用于分离具有不同电荷水平的珠子的方法和装置。
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公开(公告)号：US20180051332A9
公开(公告)日：2018-02-22
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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公开(公告)号：US09926596B2
公开(公告)日：2018-03-27
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parizi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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公开(公告)号：US20160108469A9
公开(公告)日：2016-04-21
申请号：US13481858
申请日：2012-05-27
IPC分类号： C12Q1/68 , G01N27/414 , C12M1/34
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： Aspects of the invention include methods for improving the accuracy and read length of sequencing reactions by utilizing unlabeled unincorporable nucleotides, or by rephasing colony based sequencing reactions. Other aspects include systems and devices for improved measurement of biological reactions associated with bead which may be removed, utilizing current measurement methods through the counter ions associated with said beads due to the presence of reactants bound or associated with said bead, wherein electrodes for generating and measuring said current may be within the Debye length of said bead. Other aspects of the invention include methods for determining concentrations of input samples, means for reuse of an array, methods and apparatus for separating beads with different charge levels from each other.
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6.发明授权公开(公告)号：US09187783B2
公开(公告)日：2015-11-17
申请号：US13824129
申请日：2011-10-04
IPC分类号： C12Q1/68 , G01N27/414
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.
摘要翻译： 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。
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公开(公告)号：US20140235457A1
公开(公告)日：2014-08-21
申请号：US14119859
申请日：2012-05-29
申请人： Hesaam Esfandyarpour , Kosar Baghbani Parazi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
发明人： Hesaam Esfandyarpour , Kosar Baghbani Parazi , Mark F. Oldham , Eric S. Nordman , Richard T. Reel , Susanne Baumhueter , Cheryl Heiner , Frank Lee
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , G01N27/327 , G01N33/54313 , G01N33/5438 , C12Q2527/119 , C12Q2563/116 , C12Q2565/607 , C12Q2549/126
摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.
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公开(公告)号：US20130096013A1
公开(公告)日：2013-04-18
申请号：US13632513
申请日：2012-10-01
申请人： Hesaam Esfandyarpour , Mark Oldham , Eric Nordman
发明人： Hesaam Esfandyarpour , Mark Oldham , Eric Nordman
CPC分类号： C12Q1/6874 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L2200/0652 , B01L2200/0668 , B01L2200/0689 , B01L2200/10 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/089 , B01L2300/12 , B01L2300/123 , B01L2400/0415 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0478 , B01L2400/0655 , B03C1/286 , B03C1/288 , B03C1/30 , B03C5/005 , B03C5/022 , B03C5/026 , B03C2201/26 , C12Q1/6848 , C12Q1/6853 , C12Q1/6869 , C40B60/10 , G01N1/40 , G01N27/4145 , G01N27/4146 , G01N33/5438 , G01N2001/4038 , H01L29/00 , C12Q2523/307 , C12Q2563/143 , C12Q2565/543 , C12Q2563/116 , C12Q2565/607
摘要： The present invention provides for methods and systems for Electronic DNA sequencing, single molecule DNA sequencing, and combinations of the above, providing low cost and convenient sequencing.
摘要翻译： 本发明提供电子DNA测序，单分子DNA测序及其组合的方法和系统，提供低成本和方便的测序。
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公开(公告)号：US20130029851A1
公开(公告)日：2013-01-31
申请号：US13481363
申请日：2012-05-25
CPC分类号： G01N25/4806 , B01L3/5027
摘要： A calorimeter device includes various components located on a common substrate. A first (calorimeter) integrated chip device is located on the substrate. This first device has a first microfluidic channel that has first side and a second side. A first heat sensing circuit is located on the first side of the first channel and a second heat sensing circuit is located on the second side of the channel, opposite the first side and facing the first heat sensing circuit. A second integrated chip device is located on the substrate and proximal to the first device. The second device includes a second microfluidic channel having a fourth side and fifth side. A third heat sensing circuit is located on the third side of the second channel. A fourth heat sensing circuit is located on the fourth side of the channel, opposite the third side and facing the third heat sensing circuit.
摘要翻译： 量热器装置包括位于公共基板上的各种部件。 第一（量热计）集成芯片器件位于衬底上。 该第一装置具有第一微流体通道，其具有第一侧和第二侧。 第一热感测电路位于第一通道的第一侧上，并且第二热感测电路位于通道的第二侧上，与第一侧相对并且面向第一热感测电路。 第二集成芯片器件位于衬底上并且靠近第一器件。 第二装置包括具有第四侧和第五侧的第二微流体通道。 第三热感测电路位于第二通道的第三侧。 第四热感测电路位于通道的第四侧，与第三侧相对并面对第三热感应电路。
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公开(公告)号：US08696989B2
公开(公告)日：2014-04-15
申请号：US13481363
申请日：2012-05-25
CPC分类号： G01N25/4806 , B01L3/5027
摘要： A calorimeter device includes various components located on a common substrate. A first (calorimeter) integrated chip device is located on the substrate. This first device has a first microfluidic channel that has first side and a second side. A first heat sensing circuit is located on the first side of the first channel and a second heat sensing circuit is located on the second side of the channel, opposite the first side and facing the first heat sensing circuit. A second integrated chip device is located on the substrate and proximal to the first device. The second device includes a second microfluidic channel having a third side and fourth side. A third heat sensing circuit is located on the third side of the second channel. A fourth heat sensing circuit is located on the fourth side of the channel, opposite the third side and facing the third heat sensing circuit.
摘要翻译： 量热器装置包括位于公共基板上的各种部件。 第一（量热计）集成芯片器件位于衬底上。 该第一装置具有第一微流体通道，其具有第一侧和第二侧。 第一热感测电路位于第一通道的第一侧上，并且第二热感测电路位于通道的第二侧上，与第一侧相对并且面向第一热感测电路。 第二集成芯片器件位于衬底上并且靠近第一器件。 第二装置包括具有第三侧面和第四侧面的第二微流体通道。 第三热感测电路位于第二通道的第三侧。 第四热感测电路位于通道的第四侧，与第三侧相对并面对第三热感应电路。
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