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    ANTIFREEZE PROTEIN
    1.
    发明申请
    ANTIFREEZE PROTEIN 审中-公开
    抗体蛋白

    公开(公告)号：US20130165626A1

    公开(公告)日：2013-06-27

    申请号：US13643811

    申请日：2011-04-28

    申请人： Joichi Fukuoka ,  Hidehisa Kawahara ,  Hideaki Kegasa ,  Naoki Arai ,  Jun Tomono ,  Shinichi Yokota

    发明人： Joichi Fukuoka ,  Hidehisa Kawahara ,  Hideaki Kegasa ,  Naoki Arai ,  Jun Tomono ,  Shinichi Yokota

    IPC分类号： C07K14/415 ,  C07K16/16 ,  A23L3/3472 ,  A61K8/64 ,  A61Q19/00 ,  C07K7/00 ,  C09K3/18

    CPC分类号： A61K8/645 ,  A23J3/14 ,  A23V2002/00 ,  A61K2800/52 ,  A61Q19/00 ,  C09K3/185 ,  A23V2200/206 ,  A23V2250/548

    摘要： An objective of the present invention is to provide an antifreeze protein which is capable of being efficiently produced on an industrial level at low cost and which is safe and has an excellent antifreezing activity for use on a practical level. Also, an objective of the present invention is to provide a polypeptide that corresponds to the active part of the antifreeze protein; a composition, a food, a biological sample protectant and a cosmetic containing the antifreeze protein or the polypeptide; and an antibody that specifically reacts with the antifreeze protein or the polypeptide. The antifreeze protein according to the present invention is characterized in being derived from a plant and having a specific amino acid sequence or being a plant seed protein.

    摘要翻译： 本发明的目的是提供一种能够在工业上以低成本有效地生产并且在实用水平上安全且具有优异的防冻活性的防冻蛋白。 此外，本发明的目的是提供对应于抗冻蛋白的活性部分的多肽; 组合物，食品，生物样品保护剂和含有防冻蛋白或多肽的化妆品; 以及与抗冻蛋白或多肽特异性反应的抗体。 根据本发明的抗冻蛋白的特征在于衍生自植物并具有特定氨基酸序列或植物种子蛋白。

    ICE-CRYSTAL GROWTH INHIBITING SUBSTANCE
    2.
    发明申请
    ICE-CRYSTAL GROWTH INHIBITING SUBSTANCE 审中-公开
    冰晶生长抑制物质

    公开(公告)号：US20120136138A1

    公开(公告)日：2012-05-31

    申请号：US13377405

    申请日：2010-05-27

    申请人： Hideaki Kegasa ,  Naoki Arai ,  Jun Tomono ,  Shinichi Yokota ,  Hidehisa Kawahara

    发明人： Hideaki Kegasa ,  Naoki Arai ,  Jun Tomono ,  Shinichi Yokota ,  Hidehisa Kawahara

    IPC分类号： C07K14/415 ,  C07K1/34

    CPC分类号： C07K14/415 ,  A23L3/3472 ,  A23L3/3526 ,  A23L3/36 ,  A23L33/18 ,  A61K8/645 ,  A61Q19/00

    摘要： An objective to be achieved by the present invention is to provide an ice-crystal growth inhibiting substance which has an excellent ice-crystal growth inhibiting activity suitable for practical use and which can be produced easily, efficiently and economically in safe steps usable in food production. Another objective of the present invention is to provide a method for producing the ice-crystal growth inhibiting substance, a polypeptide which is an active part of the ice-crystal growth inhibiting substance, as well as an ice-crystal growth inhibiting substance composition, a food, a biological sample protectant and a cosmetic each containing the ice-crystal growth inhibiting substance or the polypeptide. The ice-crystal growth inhibiting substance according to the present invention is characterized in that the ice-crystal growth inhibiting substance is derived from a plant, and has a molecular weight of 400 kDa or more as measured by gel filtration chromatography.

    摘要翻译： 本发明要达到的目的是提供一种冰晶生长抑制物质，它具有优良的适用于实际应用的冰晶生长抑制活性，可以容易地，高效地和经济地制备用于食品生产的安全步骤 。 本发明的另一个目的是提供一种冰晶生长抑制物质的制造方法，作为冰晶生长抑制物质的活性成分的多肽，以及冰晶生长抑制物质组合物， 食品，生物样品保护剂和含有冰晶生长抑制物质或多肽的化妆品。 本发明的冰晶生长抑制物质的特征在于，冰晶生长抑制物质来自植物，通过凝胶过滤色谱法测定分子量为400kDa以上。

    PLANT EXTRACT CONTAINING ANTIFREEZE SUBSTANCE AND METHOD FOR PRODUCING SAME
    4.
    发明申请
    PLANT EXTRACT CONTAINING ANTIFREEZE SUBSTANCE AND METHOD FOR PRODUCING SAME 审中-公开
    含有抗菌物质的植物提取物及其生产方法

    公开(公告)号：US20110278492A1

    公开(公告)日：2011-11-17

    申请号：US13146543

    申请日：2010-02-12

    申请人： Naoki Arai ,  Jun Tomono ,  Shinichi Yokota ,  Yuji Tominaga

    发明人： Naoki Arai ,  Jun Tomono ,  Shinichi Yokota ,  Yuji Tominaga

    IPC分类号： C09K3/18

    CPC分类号： A23L3/3472 ,  A23L19/00 ,  A23L19/03 ,  A23V2002/00 ,  A23V2200/206 ,  A23V2250/21

    摘要： The objective of the present invention is to provide an antifreeze substance which is suitable for practical use and has excellent antifreeze activity in a safe process flow applicable to food production in a simple, efficient and inexpensive manner. The first method for producing a plant extract containing an antifreeze substance according to the present invention comprises the steps of drying a plant containing the antifreeze substance and extracting the antifreeze substance from the dried plant. The second method for producing a plant extract containing an antifreeze substance according to the present invention comprises the steps of extracting the antifreeze substance from a plant, and treating the obtained extract from the extracting step with an adsorbent.

    摘要翻译： 本发明的目的是提供一种适用于实际应用的防冻物质，并且以简单，有效和廉价的方式在适用于食品生产的安全工艺流程中具有优异的防冻活性。 制备本发明含有防冻物质的植物提取物的第一种方法包括干燥含有防冻物质的植物并从干燥植物中提取防冻物质的步骤。 制备本发明含有防冻物质的植物提取物的第二种方法包括以下步骤：从植物中提取防冻物质，并用吸附剂处理所提取的提取物。

    Liquid food composition
    6.
    发明申请
    Liquid food composition 有权
    液体食物组成

    公开(公告)号：US20130172245A1

    公开(公告)日：2013-07-04

    申请号：US13516536

    申请日：2010-12-17

    申请人： Hiroaki Inoue ,  Yui Kawashima ,  Kazuya Hamada ,  Shinichi Yokota ,  Hiromi Maeda ,  Tatsumasa Mae ,  Jun Tomono

    发明人： Hiroaki Inoue ,  Yui Kawashima ,  Kazuya Hamada ,  Shinichi Yokota ,  Hiromi Maeda ,  Tatsumasa Mae ,  Jun Tomono

    IPC分类号： A23L1/29

    CPC分类号： A23L1/296 ,  A23L33/16 ,  A23L33/17 ,  A23L33/185 ,  A23L33/21 ,  A23L33/40 ,  A61K9/0065 ,  A61K9/10

    摘要： Provided is a liquid food composition capable of semi-solidifying in the stomach, which is a one-pack type product containing a water-soluble dietary fiber preliminarily added thereto and in the form of a liquid that can be easily taken, and stably sustains the liquid nature thereof during distribution and storage. The liquid food composition, which is capable of semi-solidifying in an acidic region, comprises a water-soluble dietary fiber (a), a specific metal compound (b), a protein (c) and an emulsifier (d), and the particle size distribution of particles contained in said liquid food composition shows two or more peaks in a neutral region.

    摘要翻译： 本发明提供一种能够在胃中半固化的液体食品组合物，其为预先添加有水溶性膳食纤维的单组分产品，并且可以容易地摄取并且稳定地维持 在分配和储存期间的液体性质。 能够在酸性区域半固化的液体食品组合物包含水溶性膳食纤维（a），特定金属化合物（b），蛋白质（c）和乳化剂（d），并且 包含在所述液体食品组合物中的颗粒的粒度分布在中性区域中显示两个或多个峰。

    Acylase gene
    7.
    发明授权
    Acylase gene 失效
    淀粉酶基因

    公开(公告)号：US07195892B2

    公开(公告)日：2007-03-27

    申请号：US10516587

    申请日：2003-05-30

    申请人： Akiko Nishi ,  Takumi Mano ,  Shinichi Yokota ,  Masayuki Takano ,  Kazuyoshi Yajima

    发明人： Akiko Nishi ,  Takumi Mano ,  Shinichi Yokota ,  Masayuki Takano ,  Kazuyoshi Yajima

    IPC分类号： C12P35/00 ,  C12P21/00 ,  C12N9/84 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12P37/04 ,  C12N9/80 ,  C12N9/84

    摘要： The subject of the present invention is to provide a β-lactam acylase protein having high activity, a gene coding for said β-lactam acylase protein, a recombinant vector having said gene, a transformant containing said recombinant vector, and a method of producing a β-lactam antibiotic such as amoxycillin using said β-lactam acylase. A β-lactam acylase gene of Stenotrophomonas maltophilia was cloned, the DNA base sequence and the amino acid sequence expected therefrom was determined, and a Stenotrophomonas β-lactam acylase gene was obtained. This gene was found to code for a protein with a molecular weight of about 70 kDa and having β-lactam acylase activity, and could efficiently produce amoxycillin without being inhibited by phenylacetic acid, etc. Furthermore, by modification of the amino acid sequence, a protein which can more efficiently produce amoxycillin could be obtained.

    摘要翻译： 本发明的主题是提供具有高活性的β-内酰胺酰基转移酶蛋白，编码所述β-内酰胺酰基转移酶蛋白的基因，具有所述基因的重组载体，含有所述重组载体的转化体，以及制备 β-内酰胺抗生素如阿莫西林，使用所述β-内酰胺酰基转移酶。 克隆了嗜麦芽寡养单胞菌的β-内酰胺酰基转移酶基因，测定了DNA碱基序列和预期的氨基酸序列，得到了嗜麦芽寡养单胞菌β-内酰胺酰基转移酶基因。 发现该基因编码具有约70kDa分子量且具有β-内酰胺酰基转移酶活性的蛋白质，并且可以有效地产生阿莫西林而不被苯乙酸等抑制。此外，通过修饰氨基酸序列，a 可以获得更有效地产生阿莫西林的蛋白质。

    Electro-sensitive working medium and method of using the medium
    9.
    发明授权
    Electro-sensitive working medium and method of using the medium 有权
    电敏工作介质和使用介质的方法

    公开(公告)号：US06455955B1

    公开(公告)日：2002-09-24

    申请号：US09673569

    申请日：2000-10-18

    申请人： Yasufumi Otsubo ,  Shinichi Yokota ,  Kazuya Edamura

    发明人： Yasufumi Otsubo ,  Shinichi Yokota ,  Kazuya Edamura

    IPC分类号： G21D702

    CPC分类号： F15B21/065 ,  C11D7/28

    摘要： The even system electricity induction operation medium of this invention has 3 halogen atoms, especially fluorine atom into a/the molecule at least it is the even system electricity induction operation medium including one kind of liquid state organic compound at least. A even system electricity induction operation medium about-the electric conduction rate under the operation environment temperature are the electricity induction operation medium that, is in the range of 4×10−10˜5×10−6S/m and the surface tension of the even system electricity induction operation medium under the operation environment temperature are 22 dyn/cm following. This even system electricity induction operation medium is difficult combustion or noninflammable nature. This even system electricity induction operation medium is difficult combustion or noninflammable. The movement style of the medium is formed among the electrode when a un-uniform electric field is formed to this electricity induction operation medium and can use this movement style to the device such as ECF motor, pump, washing device. The electricity induction operation medium of this invention is noninflammable almost because a particular halogen atom, especially fluorine compound are contained and there is not the risk of ignition even if the spark etc. results among the electrode. Also, the movement style of the electricity induction operation medium can be formed effectively because the electric conduction rate and also surface tension are in a particular range.

    摘要翻译： 本发明的均匀系统的电力诱导操作介质至少具有3个卤素原子，特别是氟原子，至少是至少包括一种液态有机化合物的均匀系统的电力诱导操作介质。 在操作环境温度下，均匀系统的电力感应操作介质是导电操作介质，其在4×10-10〜5×10-6S / m的范围内，并且偶系统的电感应的表面张力 操作介质在操作环境温度为22 dyn / cm之后。 这种均匀系统的电力感应操作介质难以燃烧或不易燃性质。 这种均匀系统的电力感应操作介质难燃或不燃。 当对该感应操作介质形成不均匀的电场时，在电极之间形成介质的运动风格，并且可以将该运动样式用于诸如ECF电动机，泵，清洗装置的装置。 本发明的电感应操作介质几乎是因为含有特定的卤素原子，特别是氟化合物而不易燃，即使在电极中产生火花等，也不存在着火的危险。 此外，由于导电率和表面张力在特定的范围内，所以可以有效地形成电力感应操作介质的移动风格。