公开(公告)号：US4839279A

公开(公告)日：1989-06-13

申请号：US749425

申请日：1985-06-27

申请人： Akira Kosaka ,  Sawao Murao ,  Kenichi Hirano ,  Noriaki Tanaka ,  Kuniyoshi Matsunaga

发明人： Akira Kosaka ,  Sawao Murao ,  Kenichi Hirano ,  Noriaki Tanaka ,  Kuniyoshi Matsunaga

IPC分类号： C12Q1/26 ,  C12Q1/54 ,  C12Q1/60 ,  C12Q1/62

CPC分类号： C12Q1/62 ,  C12Q1/26 ,  C12Q1/54 ,  C12Q1/60 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/825 ,  Y10T436/103332 ,  Y10T436/144444 ,  Y10T436/146666 ,  Y10T436/25125

摘要： A novel enzyme of bilirubin oxidase and a conventional enzyme of laccase are found to oxidize bilirubin to biliverdin without formation of hydrogen peroxide, thereby permitting the content of conjugated bilirubin in biological fluid to be quantitatively determined by reacting therewith the bilirubin oxidase of a microorganism of genus Myrothecium origin, and also permitting the content of total bilirubin in biological fluid to be quantitatively determined by reacting therewith such a bilirubin oxidase or laccase in the presence of a specific additive compound.

摘要翻译： 发现胆红素氧化酶的新型酶和漆酶的常规酶将胆红素氧化成胆绿素而不形成过氧化氢，从而通过与生物液体中的微生物的胆红素氧化酶反应来定量地测定生物液中的共轭胆红素含量 通过与特定的添加剂化合物的存在下的胆红素氧化酶或漆酶反应，可以定量测定生物体液中总胆红素的含量。

公开(公告)号：US4554249A

公开(公告)日：1985-11-19

申请号：US436385

申请日：1982-10-25

申请人： Akira Kosaka ,  Sawao Murao ,  Kenichi Hirano ,  Noriaki Tanaka ,  Kuniyoshi Matsunaga

发明人： Akira Kosaka ,  Sawao Murao ,  Kenichi Hirano ,  Noriaki Tanaka ,  Kuniyoshi Matsunaga

IPC分类号： C12Q1/26 ,  C12Q1/54 ,  C12Q1/60 ,  C12Q1/62 ,  C07G17/00 ,  C12N9/02

CPC分类号： C12Q1/62 ,  C12Q1/26 ,  C12Q1/54 ,  C12Q1/60 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/825 ,  Y10T436/103332 ,  Y10T436/144444 ,  Y10T436/146666 ,  Y10T436/25125

摘要： A novel enzyme of bilirubin oxidase produced by a genus Myrothecium or genus Coprinus origin microorganism and a conventional enzyme of laccase are found, in the presence of a specific additive compound, e.g. a surface active agent, aromatic carboxylic acid, sulfa drug or protease, to oxidize both conjugated and unconjugated bilirubin in biological fluid to biliverdin without formation of hydrogen peroxide, such that in the case of conventional enzymatic methods of the quantitative determination of glucose, cholesterol, neutral fats, free fatty acids, phospholipids or uric acid all existing together with bilirubin in biological fluid, the usual interference with such determination, as otherwise caused by bilirubin coexisting in such fluid, can be prevented by adding such a bilirubin oxidase or laccase together with such a specific additive compound to the determinative reaction system.

摘要翻译： 在特定的添加剂化合物的存在下，发现了由黑曲霉属或鬼伞属起源微生物和常规酶漆酶产生的胆红素氧化酶的新型酶。 表面活性剂，芳族羧酸，磺胺药物或蛋白酶，以将生物液体中的共轭和非共轭胆红素氧化成胆绿素而不形成过氧化氢，使得在常规的酶法测定葡萄糖，胆固醇， 中性脂肪，游离脂肪酸，磷脂或尿酸都与生物液体中的胆红素一起存在，通过将这种胆红素共存在这种流体中的其他方式引起的这种测定的通常干扰可以通过将这样的胆红素氧化酶或漆酶与 这种特定的添加剂化合物与决定性反应体系。

公开(公告)号：US4294754A

公开(公告)日：1981-10-13

申请号：US27029

申请日：1979-04-04

申请人： Yoshiyuki Takahara ,  Yoshiteru Hirose ,  Yoko Takeuchi ,  Asao Murai ,  Masatsune Kainosho ,  Sawao Murao

发明人： Yoshiyuki Takahara ,  Yoshiteru Hirose ,  Yoko Takeuchi ,  Asao Murai ,  Masatsune Kainosho ,  Sawao Murao

IPC分类号： A61K35/74 ,  A61K38/00 ,  A61P31/04 ,  C07K7/06 ,  C07K11/00 ,  C07K11/02 ,  C07K14/195 ,  C07K14/41 ,  C12P21/04 ,  C07C103/52

CPC分类号： C07K7/06 ,  C12R1/09 ,  Y10S930/19 ,  Y10S930/27

摘要： A purified ring peptide antibiotic of the following empirical formula:N.alpha.-(3-hydroxy-4-methyl-1-oxohexyl)-L-.alpha.,.gamma.-diaminobutyryl-L-isoleucyl-L-.alpha.,.gamma.-diaminobutyryl-D-phenylalanyl-L-leucyl-L-.alpha.,.gamma.-diaminobutyryl-D-valyl-L-leucyl-L-serine (9-1)-lactoneand which has the following structural formula: ##STR1## and wherein Dab represents 2,4-diamino butyric acid.

摘要翻译： 具有以下经验式的纯化的环肽抗生素：Nα-（3-羟基-4-甲基-1-氧代己基）-L-α，γ-二氨基丁酰基-L-异亮氨酰-L-α，γ-二氨基丁酰基-D- 苯丙氨酰-L-亮氨酰-L-α，γ-二氨基丁酰-D-缬氨酰-L-亮氨酰-L-丝氨酸（9-1） - 内酯，其具有以下结构式：其中Dab表示2,4 - 二氨基丁酸。

公开(公告)号：US5494812A

公开(公告)日：1996-02-27

申请号：US274896

申请日：1994-07-14

申请人： Sawao Murao ,  Takashi Shin ,  Kyoichi Sugawa ,  Amachi Teruo

发明人： Sawao Murao ,  Takashi Shin ,  Kyoichi Sugawa ,  Amachi Teruo

IPC分类号： A01N63/02 ,  A01N63/00 ,  C07G99/00 ,  C12N9/99 ,  C12P1/06 ,  C12R1/465 ,  C12N1/20 ,  C12N9/98

CPC分类号： A01N63/00 ,  C12P1/06 ,  Y10S435/886

摘要： A novel trehalase inhibitor having the following physicochemical properties:(1) Activity: a specific inhibitory activity to trehalase;(2) Form: white powder, water-soluble;(3) Somogy-Nelson test: negative(4) Rydon-Smith test: positive(5) Optical rotation [.alpha.].sub.D.sup.23 : 113.8.degree. (C, 0.1, H.sub.2 O)(6) Molecular weight: 335 ([M+H].sup.+ =336)(7) Ultraviolet absorption spectrum: 258.5 nm (max.)(8) .sup.13 C-NMR(ppm): 28.9, 41.0, 56.9, 58.4, 64.3, 65.0, 71.3, 74.0, 75.2, 75.8, 76.0, 77.1, 123.0, 144.7

摘要翻译： 具有以下物理化学性质的新型海藻糖酶抑制剂：（1）活性：对海藻糖酶的特异性抑制活性; （2）形式：白色粉末，水溶性; （3）Somogy-Nelson测试：阴性（4）Rydon-Smith测试：阳性（5）旋光度D23：113.8°（C，0.1，H 2 O）（6）分子量335（[M + H] + = 336）（7）紫外吸收光谱：258.5nm（最大）（8）13 C-NMR（ppm）：28.9,41.0,56.9,58.4,64.3,65.0,71.3,74.0,75.02,75.8,76.0,77.1 ，123.0,144。

公开(公告)号：US4059487A

公开(公告)日：1977-11-22

申请号：US559462

申请日：1975-03-18

申请人： Toyokazu Nishino ,  Yasutaro Hamagishi ,  Toshikazu Oki ,  Sawao Murao

发明人： Toyokazu Nishino ,  Yasutaro Hamagishi ,  Toshikazu Oki ,  Sawao Murao

IPC分类号： A61K38/45 ,  C07H19/20 ,  C12N9/12 ,  C12P19/32 ,  C12D13/06

CPC分类号： A61K38/45 ,  C07H19/20 ,  C12N9/12 ,  C12P19/32 ,  Y10S435/826 ,  Y10S435/886

摘要： New purine nucleoside 5'-phosphate (mono, di or tri) 3'(2')-diphosphates and the sodium, lithium and potassium salts thereof are elaborated by the enzymatic transfer of pyrophosphoryl group from ATP (adenosine triphosphate), dATP (deoxyadenosine triphosphate) and pppApp (adenosine 5'-triphosphate 3'(2')diphosphate) to specified 5'-purine nucleotides, and a new nucleotide pyrophosphotransferase used for this enzyme reaction is produced by actinomycetes and other microorganisms and recovered from the culture filtrate and mycelium by the conventional methods for recovering enzymes.

摘要翻译： 新的嘌呤核苷5'-磷酸（1，二或三）3'（2'） - 二磷酸盐及其钠，锂和钾盐通过从ATP（三磷酸腺苷），dATP（脱氧腺苷） 三磷酸盐）和pppApp（腺苷5'-三磷酸3'（2'）二磷酸）到特定的5'-嘌呤核苷酸，并且用于该酶反应的新的核苷酸焦磷酸转移酶由放线菌和其他微生物产生，并从培养滤液和 菌丝体通过常规方法回收酶。

公开(公告)号：US5354685A

公开(公告)日：1994-10-11

申请号：US40184

申请日：1993-03-29

申请人： Sawao Murao ,  Takashi Shin ,  Kyoichi Sugawa ,  Amachi Teruo

发明人： Sawao Murao ,  Takashi Shin ,  Kyoichi Sugawa ,  Amachi Teruo

IPC分类号： A01N63/02 ,  A01N63/00 ,  C07G99/00 ,  C12N9/99 ,  C12P1/06 ,  C12R1/465 ,  C12N1/20 ,  C12N9/98

CPC分类号： A01N63/00 ,  C12P1/06 ,  Y10S435/886

摘要： A novel trehalase inhibitor having the following physicochemical properties:(1) Activity: a specific inhibitory activity to trehalase;(2) Form: white powder, water-soluble;(3) Somogy-Nelson test: negative(4) Rydon-Smith test: positive(5) Optical rotation [.alpha.].sub.D.sup.23 : 113.8.degree. (C, 0.1 H.sub.2 O)(6) Molecular weight: 335 ([M+H].sup.+ =336)(7) Ultraviolet absorption spectrum: 258.5 nm (max.)(8) .sup.13 C-NMR(ppm): 28.9, 41.0, 56.9, 58.4, 64.3, 65.0, 71.3, 74.0, 75.2, 75.8, 76.0, 77.1, 123.0, 144.7

摘要翻译： 具有以下物理化学性质的新型海藻糖酶抑制剂：（1）活性：对海藻糖酶的特异性抑制活性; （2）形式：白色粉末，水溶性; （3）Somogy-Nelson测试：阴性（4）Rydon-Smith测试：阳性（5）旋光度D23：113.8℃（C，0.1H 2 O）（6）分子量：335（[M + H] + = 336）（7）紫外吸收光谱：258.5nm（最大）（8）13 C-NMR（ppm）：28.9,41.0,56.9,58.4,64.3,65.0,71.3,74.0,75.02,75.8,76.0,77.1， 123.0,144。

公开(公告)号：US4010258A

公开(公告)日：1977-03-01

申请号：US557555

申请日：1975-03-12

申请人： Sawao Murao

发明人： Sawao Murao

IPC分类号： A61K31/715 ,  C12P19/04 ,  A61K35/00 ,  G07F3/02

CPC分类号： A61K31/715 ,  C12P19/04 ,  Y10S435/886

摘要： A new amylase inhibitor, called Amylostatin-A, which can be distinguished from those disclosed heretofore on the basis of its constitution, molecular weight, specificity for certain types of amylase and adsorbability, is obtained by cultivating a strain of actinomycetes belonging to Streptomyces diastaticus var. amylostaticus FERM P-2499 in a medium containing starch, preferably amylopectin, as the sole source of carbon; the conditions of cultivation, methods of isolation and purification and therapeutical application thereof are disclosed.

摘要翻译： 基于其构成，分子量，某些类型的淀粉酶的特异性和吸附性，可以区别于迄今为止公开的新淀粉酶抑制剂，即淀粉酶抑制剂A，通过培养属于链霉菌属（Streptomyces diastaticus var）的放线菌菌株 。 淀粉FERM P-2499在含有淀粉的培养基中，优选支链淀粉作为唯一的碳源; 公开了培养条件，分离和纯化方法及其治疗应用。

公开(公告)号：US5372816A

公开(公告)日：1994-12-13

申请号：US948236

申请日：1992-09-21

申请人： Sawao Murao ,  Takashi Shin

发明人： Sawao Murao ,  Takashi Shin

IPC分类号： A01N63/00 ,  A01N63/02 ,  C07H7/02 ,  C07H7/06 ,  C07H17/08 ,  C07H19/02 ,  C07H19/24 ,  C12N1/20 ,  C12N9/99 ,  C12P19/12 ,  C12P19/26 ,  C12P19/28 ,  C07H5/04 ,  C12P7/00

CPC分类号： C07H17/08 ,  A01N63/00 ,  C07H19/02 ,  C07H19/24 ,  C12P19/26 ,  C12P19/28 ,  C12R1/04 ,  Y10S435/822 ,  Y10S435/911

摘要： This invention relates to the substance Trehalostatin which is a white powder soluble in water but hardly or only slightly soluble in hexane, benzene, ethers and petroleum ether, shows no absorption maxima at 220 nm or above in its ultraviolet visible light absorption spectrum, is positive in Rydon-Smith reaction and negative in nynhydrin reaction, 3,6-dinitrophthalic acid reaction and Elson-Morgan reaction, and has an RF value of 0.37 in Merck Kieselgal 50 F.sub.254 thin-layer chromatography using 3:1:2 mixture on n-butanol, acetic acid and water as a developing solvent, Rt of 11.0 minutes in YMC PA03 (0.7 .times.27 cm) high performance liquid chromatography using 65% v/v acetonitrile (in H.sub.2 O) as a solvent at a flow rate of 1.0 ml/min, a molecular weight of 366, [alpha].sub.D of +115.degree., and specific NMR spectrum.Also disclosed is a process for preparing trehalostatin and a specific strain of Amycolatopsis trehalostatica which has FERM accession number BP-2784.In addition the trehalostatin compound shows an inhibitory effect against trehalase in insects, especially Aldrichina grahami, even at a very low concentration and is therefore useful as an insecticide.

摘要翻译： 本发明涉及物质Trehalostatin，其为白色粉末，可溶于水，但几乎或仅微溶于己烷，苯，醚和石油醚，其紫外可见光吸收光谱在220nm或以上无最大吸收，为阳性 在Rydon-Smith反应中，在Nynhydrin反应中为负，3,6-二硝基邻苯二甲酸反应和Elson-Morgan反应，并且在Merck Kieselgal 50 F254薄层色谱法中的RF值为0.37，使用3：1： 丁醇，乙酸和水作为显影溶剂，使用65％v / v乙腈（在H 2 O中）作为溶剂，以1.0ml / min的流速在YMC PA03（0.7×27cm）高效液相色谱中Rt为11.0分钟 ，分子量为366，αD为+115°，具体NMR谱。 还公开了一种制备具有FERM登录号BP-2784的海藻抑素和一种特异菌株的嗜酸沙门氏菌纲的方法。 此外，即使在非常低的浓度下，海藻抑素化合物也显示出对昆虫，特别是Aldrichina grahami中海藻糖酶的抑制作用，因此可用作杀虫剂。

公开(公告)号：US5169778A

公开(公告)日：1992-12-08

申请号：US601738

申请日：1990-12-06

申请人： Sawao Murao ,  Takashi Shin

发明人： Sawao Murao ,  Takashi Shin

IPC分类号： A01N63/00 ,  A01N63/02 ,  C07H7/02 ,  C07H7/06 ,  C07H17/08 ,  C07H19/02 ,  C07H19/24 ,  C12N1/20 ,  C12N9/99 ,  C12P19/12 ,  C12P19/26 ,  C12P19/28

CPC分类号： C07H17/08 ,  A01N63/00 ,  C07H19/02 ,  C07H19/24 ,  C12P19/26 ,  C12P19/28 ,  C12R1/04 ,  Y10S435/822 ,  Y10S435/911

摘要： This invention relates to a substance Trehalostatin which is a white powder soluble in water but hardly or only slightly soluble in hexane, benzene, ethers and petroleum ether, shows no absorption maxima at 220 nm or above in its ultraviolet visible light absorption spectrum, is positive in Rydon-Smith reaction and negative in ninhydrin reaction, 3,6-dinitrophthalic acid reaction and Elson-Morgan reaction, has an Rf value of 0.37 in Merk Kieselgal 60 G.sub.254 thin layer chromatography using a 3:1:2 mixture of n-butanol, acetic acid and water as a developing solvent, and Rt of 11.0 minutes in YMC PA03 (0.7.times.27 cm) high performance liquid chromatography using 65% v/v acetonitrile (in H.sub.2 O) as a solvent at a flow rate of 1.0 ml/min, has a molecular weight of 366 and a specific rotatory power [.alpha.].sub.D of +115.degree., and presents an NMR spectrum described below:H-NMR/D.sub.2 O 3.3 (ppm), dd, 1H, 3.5 (ppm), m, 1H, 3.5 (ppm), t, 1H, 3.6 (ppm), d, 1H, 3.6 (ppm), d, 1H, 3.7 (ppm), d, 1H, 3.7 (ppm), d, 1H, 3.8 (ppm), ddd, 1H, 4.1 (ppm), dd, 1H, 4.2 (ppm), d, 1H, 4.8 (ppm), ddd, 1H, 5.2 (ppm), d, 1H,.sup.13 C-NMR/D.sub.2 O: 63.5 (ppm), 64.8 (ppm), 72.4 (ppm), 72.8 (ppm), 74.8 (ppm), 75.8 (ppm), 76.2 (ppm), 83.0 (ppm), 83.2 (ppm), 83.4 (ppm), 85.6 (ppm), 89.9 (ppm), 163.8 (ppm),to a process for preparing said substance, and to actinomycetes capable of producing said substance.Trehalostatin of the present invention shows an inhibitory effect against trehalase in insects, especially Aldrichina grahami, even at a very low concentration and is therefore useful as an insecticide.

公开(公告)号：US4769323A

公开(公告)日：1988-09-06

申请号：US688423

申请日：1985-01-02

申请人： Sawao Murao ,  Toyokazu Nishino

发明人： Sawao Murao ,  Toyokazu Nishino

IPC分类号： C12N9/99 ,  C12Q1/37 ,  C12Q1/52 ,  C12N9/10 ,  C12R1/465

CPC分类号： C12N9/99 ,  C12Q1/52 ,  Y10S435/81 ,  Y10S435/886

摘要： A method of inactivating cytosolic aspartate aminotransferase isozyme comprises addition of a specific inhibitory enzyme.A method for the fractional determination of aspartate aminotransferase isozyme activities, which comprises (a) inactivating the cytosolic aspartate aminotransferase isozyme in a reaction mixture containing the specimen by the addition of a specific inhibitory enzyme, followed by determination of the residual mitochondrial aspartate aminotransferase isozyme activity, and (b) determination of the cytosolic isozyme activity by subtracting the activity of mitochondrial isozyme determined in (a) from the total activity of aspartate aminotransferase isozymes.A cytosolic aspartate aminotransferase isozyme inhibiting composition contains an effective cytosolic aspartate aminotransferase isozyme inhibitory amount of a specific inhibitory enzyme.

摘要翻译： 灭活胞质天冬氨酸氨基转移酶同工酶的方法包括加入特异性抑制酶。 一种天冬氨酸氨基转移酶同工酶活性的分级测定方法，其特征在于：（a）通过加入特异性抑制酶使包含标本的反应混合物中的胞质内天冬氨酸氨基转移酶同工酶失活，然后测定残留的线粒体天冬氨酸氨基转移酶同工酶活性 ，和（b）通过从天冬氨酸氨基转移酶同功酶的总活性中减去（a）中测定的线粒体同功酶的活性来测定细胞溶质同功酶活性。 胞质天冬氨酸氨基转移酶同功酶抑制组合物含有有效的胞质内天冬氨酸氨基转移酶同功酶抑制量的特异性抑制酶。