公开(公告)号：US07638035B2

公开(公告)日：2009-12-29

申请号：US12363118

申请日：2009-01-30

申请人： Hidehiro Sasaki ,  Akio Oki

发明人： Hidehiro Sasaki ,  Akio Oki

IPC分类号： G01N27/30

CPC分类号： G01N27/3277

摘要： An object of the invention is to provide an electrode plate for electrochemical measurements for detecting with high sensitivity and determining a substance included in a living body. The electrode plate of the present invention has on both faces of body of the substrate, oxidation electrode and reduction electrode opened respectively at upper layer opening and lower layer opening having the same area; and further has a plurality of through-holes that penetrate through from the face of the oxidation electrode to the face of the reduction electrode, in which electrode pairs are formed which exhibit a redox cycle effect between the oxidation electrode and the reduction electrode by applying the potential which can proceed an oxidative reaction of a reductant on the oxidation electrode, and the potential which can proceed a reductive reaction of an oxidant on the reduction electrode.

摘要翻译： 本发明的目的是提供一种用于电化学测量的电极板，用于高灵敏度地检测并确定生物体中所含的物质。 本发明的电极板在基板的两面，氧化电极和还原电极分别在相同面积的上层开口和下层开口处开口; 并且还具有从氧化电极的表面贯通到还原电极的表面的多个通孔，其中形成电极对，在氧化电极和还原电极之间施加氧化还原循环效应， 能够进行还原剂在氧化电极上的氧化反应的电位，以及可以进行还原电极上的氧化剂的还原反应的电位。

公开(公告)号：US20090145780A1

公开(公告)日：2009-06-11

申请号：US12363118

申请日：2009-01-30

申请人： Hidehiro Sasaki ,  Akio Oki

发明人： Hidehiro Sasaki ,  Akio Oki

IPC分类号： G01N27/26

CPC分类号： G01N27/3277

摘要： An object of the invention is to provide an electrode plate for electrochemical measurements for detecting with high sensitivity and determining a substance included in a living body in a slight amount.The electrode plate for electrochemical measurements 10 of the present invention has on both faces of body of the substrate 32S, oxidation electrode 32W and reduction electrode 32w opened respectively at upper layer opening 31W and lower layer opening 33w having the same area; and further has a plurality of through-holes 32H that penetrate through from the superior face of the oxidation electrode 32W to the inferior face of the reduction electrode 32w, in which electrode pairs are formed which exhibit a redox cycle effect between the oxidation electrode 32W and the reduction electrode 32w by applying the potential which can proceed an oxidative reaction of a reductant on the oxidation electrode 32W, and the potential which can proceed a reductive reaction of an oxidant on the reduction electrode 32w.

摘要翻译： 本发明的目的是提供一种用于电化学测量的电极板，用于高灵敏度地检测并且以微量的方式确定包括在生物体中的物质。 本发明的电化学测定用电极板10具有分别在上层开口部31W开口的基板32S，氧化电极32W和还原电极32w的两面以及相同面积的下层开口部33w。 并且还具有从氧化电极32W的上表面穿过的多个贯通孔32H到还原电极32w的下表面，其中形成有在氧化电极32W与氧化电极32W之间具有氧化还原循环效应的电极对 通过施加可以进行氧化电极32W上的还原剂的氧化反应的电位的还原电极32w以及可以进行还原电极32w上的氧化剂的还原反应的电位。

公开(公告)号：US08062489B2

公开(公告)日：2011-11-22

申请号：US12971885

申请日：2010-12-17

申请人： Akio Oki ,  Norihito Tsukahara ,  Masato Suzuki ,  Hiroaki Oka

发明人： Akio Oki ,  Norihito Tsukahara ,  Masato Suzuki ,  Hiroaki Oka

IPC分类号： G01N27/327 ,  G01N33/92 ,  B01L3/00

CPC分类号： G01N33/48728

摘要： An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution. The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 μl. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; adding a lipid solution to the artificial lipid membrane forming portion; adding to the second chamber a second electrolytic solution having a viscosity of not lower than 1.3 mPa·s and not higher than 200 mPa·s; and forming the artificial lipid membrane.

摘要翻译： 本发明的目的是提供一种在抑制电解液的泄漏和蒸发的同时稳定地形成人造脂膜的方法。 本发明是使用人造脂质膜形成装置形成人造脂质膜的人造脂质膜形成方法。 人造脂膜形成装置包括第一室，第二室，分隔壁和人造脂膜形成部。 第一室和第二室中的每一个具有不小于10pl且不大于200μl的容量。 本发明的人造脂膜形成方法包括以下步骤：制备人造脂膜形成装置; 向第一室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第一电解液; 向人造脂膜形成部分添加脂质溶液; 向第二室中加入粘度不低于1.3mPa·s且不高于200mPa·s的第二电解液; 并形成人造脂膜。

公开(公告)号：US20080028821A1

公开(公告)日：2008-02-07

申请号：US11659599

申请日：2005-08-09

申请人： Yasuhiro Horiike ,  Akio Oki

发明人： Yasuhiro Horiike ,  Akio Oki

IPC分类号： G01N33/48 ,  B04B5/02 ,  G01N27/26 ,  G01N35/00 ,  G01N27/333

CPC分类号： B01L3/502753 ,  A61B5/150022 ,  A61B5/150213 ,  A61B5/150221 ,  A61B5/150389 ,  A61B5/150503 ,  A61B5/15142 ,  A61B5/15186 ,  B01L2200/14 ,  B01L2200/148 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0409 ,  G01N27/4163 ,  G01N35/00693 ,  G01N2035/00495 ,  Y10T436/111666

摘要： A corpuscle/plasma separating part is disposed at the lower end of the substrate, and a sensor part connected to the corpuscle/plasma separating part is disposed at the upper end of the substrate, with a calibration solution reservoir being disposed on the lower side of the sensor part, and a calibration solution waste reservoir being disposed on the upper side of the sensor part. A first centrifugal axis is located upper to the corpuscle fraction storing part and lower to the plasma fraction storing part of the corpuscle/plasma separating part, while a second centrifugal axis is located within or close to the sensor part. Conveyance and discharge of the calibration solution can be carried out by performing centrifugation around the first centrifugal axis which is distant from the sensor part at a low speed of rotation, so that the centrifugal force exerted on the sensors would be small. During the centrifuge operation at a high speed of rotation for the separation of blood corpuscles, centrifugation can be performed around the second centrifugal axis so that the centrifugal force exerted on the sensors is small. Centrifuge operation allows separation of the blood corpuscles and blood plasma, and conveyance of the blood plasma and the calibration solution, as Well as certain discharge of the calibration solution from the sensors, thereby allowing precise analysis. Any damage in the sensors due to strong centrifugal force during the separation of blood corpuscles and blood plasma can be prevented.

摘要翻译： 微粒/等离子体分离部设置在基板的下端，并且连接到微粒/等离子体分离部的传感器部分设置在基板的上端，校准溶液储存器设置在基板的下侧 传感器部分和校准溶液废液储存器设置在传感器部分的上侧。 第一离心轴位于微粒分数存储部的上方，并且位于小体/等离子体分离部的等离子体馏分存储部的上方，而第二离心轴位于传感器部内或其附近。 校准溶液的输送和排出可以通过围绕离开传感器部分的低速旋转的第一离心轴进行离心而进行，使得施加在传感器上的离心力将变小。 在用于分离血球的高速旋转离心机操作期间，可以围绕第二离心轴进行离心，使得施加在传感器上的离心力小。 离心机操作允许分离血小板和血浆，以及血浆和校准溶液的输送，以及校准溶液从传感器中的某些放电，从而允许精确的分析。 可以防止在分离血液和血浆期间强力离心力导致的传感器损伤。

公开(公告)号：US07828947B2

公开(公告)日：2010-11-09

申请号：US12772558

申请日：2010-05-03

申请人： Akio Oki ,  Hiroaki Oka

发明人： Akio Oki ,  Hiroaki Oka

IPC分类号： G01N27/327 ,  G01N33/92 ,  B01L3/00

CPC分类号： G01N33/6872 ,  B01L3/502707 ,  B01L3/502769

摘要： An object of the present invention is to provide a method for easily forming an artificial lipid membrane in a short period of time and an artificial lipid membrane forming apparatus suitable for carrying out such method.The present invention relates to a method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The apparatus comprises a substrate, a first spacer, a first thin film, a second spacer, a second thin film, and a cover, wherein a first chamber is formed between the substrate and the first thin film, the first thin film has a first through hole, a second chamber is formed between the first thin film and the second thin film, the second thin film has a second through hole, and the cover has an inlet. The method includes in this order a first electrolytic solution pouring step of pouring an electrolytic solution to the first chamber, a lipid solution pouring step of pouring a lipid solution to the first through hole or the second through hole, and a second electrolytic solution pouring step of pouring the electrolytic solution to the inlet to form the artificial lipid membrane inside the through hole to which the lipid solution is poured.

摘要翻译： 本发明的目的是提供一种在短时间内容易地形成人造脂质膜的方法和适于实施这种方法的人造脂膜形成装置。 本发明涉及使用人造脂膜形成装置形成人造脂膜的方法。 所述装置包括基板，第一间隔件，第一薄膜，第二间隔件，第二薄膜和盖子，其中在所述基板和所述第一薄膜之间形成第一腔室，所述第一薄膜具有第一 通孔，在第一薄膜和第二薄膜之间形成第二室，第二薄膜具有第二通孔，盖具有入口。 该方法包括以下顺序：向第一室注入电解液的第一电解溶液倾倒步骤，将脂质溶液倒入第一通孔或第二通孔的脂质溶液注入步骤，以及第二电解液倾倒步骤 将电解液注入入口，以在通过该孔注入脂质溶液之前形成人造脂质膜。

公开(公告)号：US08056395B2

公开(公告)日：2011-11-15

申请号：US12957890

申请日：2010-12-01

申请人： Akio Oki

发明人： Akio Oki

IPC分类号： G01N27/70

CPC分类号： B03C3/017 ,  B03C3/014 ,  B03C3/383 ,  B03C3/68 ,  B03C2201/24 ,  B03C2201/26 ,  B03C2201/32 ,  G01N1/4022 ,  G01N2001/4033

摘要： The present invention provides a method for detecting easily and efficiently a chemical substance contained in a gas sample at an ultralow amount.The present invention is directed to detecting method of a chemical substance contained in a gas sample, using an analyzing device with electrostatic atomizer. The analyzing device comprises a vessel, a inlet, a cooling part, an atomizing electrode, a counter electrode, an intermediate electrode, a liquid detecting part, and a detecting electrode. According to a detecting method of the present invention, the gas sample is condensed as a first condensate liquid at the surface of the atomizing electrode. The first condensate liquid is configured to be electric-charged fine particles to obtain a second condensate liquid at the surface of the counter electrode. The resulted second condensate liquid is brought in contact with the detecting electrode and a current voltage is applied between the counter electrode and the detecting electrode. The chemical substance is detected on the basis of the generated current value.

摘要翻译： 本发明提供一种容易且高效地检测气体样品中含有的化学物质的方法。 本发明涉及使用具有静电雾化器的分析装置来检测包含在气体样品中的化学物质的方法。 分析装置包括容器，入口，冷却部分，雾化电极，对电极，中间电极，液体检测部分和检测电极。 根据本发明的检测方法，气体样品在雾化电极的表面作为第一冷凝液冷凝。 第一冷凝液被配置为带电的微粒，以在对电极的表面获得第二冷凝液。 所得到的第二冷凝液与检测电极接触，并且在对电极和检测电极之间施加电流电压。 根据产生的电流值检测化学物质。

公开(公告)号：US07678577B2

公开(公告)日：2010-03-16

申请号：US11659599

申请日：2005-08-09

申请人： Yasuhiro Horiike ,  Akio Oki

发明人： Yasuhiro Horiike ,  Akio Oki

IPC分类号： G01N9/30 ,  B01D21/00 ,  B01D21/26 ,  B01L3/02 ,  B01L11/00 ,  B01L3/00 ,  B01L9/00 ,  G01N21/00

CPC分类号： B01L3/502753 ,  A61B5/150022 ,  A61B5/150213 ,  A61B5/150221 ,  A61B5/150389 ,  A61B5/150503 ,  A61B5/15142 ,  A61B5/15186 ,  B01L2200/14 ,  B01L2200/148 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0409 ,  G01N27/4163 ,  G01N35/00693 ,  G01N2035/00495 ,  Y10T436/111666

摘要： A corpuscle/plasma separating part is disposed at the lower end of the substrate, and a sensor part connected to the corpuscle/plasma separating part is disposed at the upper end of the substrate, with a calibration solution reservoir being disposed on the lower side of the sensor part, and a calibration solution waste reservoir being disposed on the upper side of the sensor part. A first centrifugal axis is located upper to the corpuscle fraction storing part and lower to the plasma fraction storing part of the corpuscle/plasma separating part, while a second centrifugal axis is located within or close to the sensor part. Conveyance and discharge of the calibration solution can be carried out by performing centrifugation around the first centrifugal axis which is distant from the sensor part at a low speed of rotation, so that the centrifugal force exerted on the sensors would be small. During the centrifuge operation at a high speed of rotation for the separation of blood corpuscles, centrifugation can be performed around the second centrifugal axis so that the centrifugal force exerted on the sensors is small. Centrifuge operation allows separation of the blood corpuscles and blood plasma, and conveyance of the blood plasma and the calibration solution, as well as certain discharge of the calibration solution from the sensors, thereby allowing precise analysis. Any damage in the sensors due to strong centrifugal force during the separation of blood corpuscles and blood plasma can be prevented.

摘要翻译： 微粒/等离子体分离部设置在基板的下端，并且连接到微粒/等离子体分离部的传感器部分设置在基板的上端，校准溶液储存器设置在基板的下侧 传感器部分和校准溶液废液储存器设置在传感器部分的上侧。 第一离心轴位于微粒分数存储部的上方，并且位于小体/等离子体分离部的等离子体部分存储部的上方，而第二离心轴位于传感器部内或靠近传感器部。 校准溶液的输送和排出可以通过围绕离开传感器部分的低速旋转的第一离心轴进行离心而进行，使得施加在传感器上的离心力将变小。 在用于分离血球的高速旋转离心机操作期间，可以围绕第二离心轴进行离心，使得施加在传感器上的离心力小。 离心机操作允许血细胞和血浆分离，血浆和校正溶液的输送，以及校准溶液从传感器中的一定排放，从而允许精确的分析。 可以防止在分离血液和血浆期间强力离心力导致的传感器损伤。

公开(公告)号：US08097013B2

公开(公告)日：2012-01-17

申请号：US12827472

申请日：2010-06-30

申请人： Akio Oki ,  Hiroaki Oka

发明人： Akio Oki ,  Hiroaki Oka

IPC分类号： A61B17/34

CPC分类号： A61B5/15186 ,  A61B5/150022 ,  A61B5/150068 ,  A61B5/150335 ,  A61B5/150389 ,  A61B5/150427 ,  A61B5/150442 ,  A61B5/150503 ,  A61B5/150541 ,  A61B5/150618 ,  A61B5/150717 ,  A61B5/15113 ,  A61B5/15117 ,  A61B5/1519 ,  A61B5/157 ,  A61B5/411

摘要： Provided are skin incision instrument to efficiently incise minimal portions and a method for incising skin with the skin incision instrument. The skin incision instrument according to the present invention comprises a holder, a needle, a needle drive unit, two or more skin expander sets, and a reader, wherein the skin expander sets comprise a first skin expander and a second skin expander; the first skin expander and the second skin expander are capable of expanding skin at both sides of the linear incision portion away from the linear incision portion and expanding the linear incision portion in a direction to expand the linear incision portion; and, minimal portions are efficiently incised by selecting a single skin expander set from two or more of the skin expander set based on the direction of the linear incision portion, such that an angle is adjusted to 45 degrees or more and 90 degrees or less wherein the angle is formed by the direction of the linear incision portion read by the reader and the direction to expand the skin by the single skin expander set.

摘要翻译： 提供有效切割最小部分的皮肤切割仪器和用皮肤切开仪器切割皮肤的方法。 根据本发明的皮肤切开仪器包括保持器，针头，针驱动单元，两个或更多个皮肤膨胀器组和读取器，其中所述皮肤膨胀器组包括第一皮肤膨胀器和第二皮肤膨胀器; 第一皮肤扩张器和第二皮肤扩张器能够使直线切口部分的两侧的皮肤远离线性切口部分，并使线性切口部分沿着扩大线性切口部分的方向膨胀; 并且通过基于线性切口部分的方向从两个或更多个皮肤扩张器组中选择单个皮肤扩张器组，使得角度被调节到45度以上且90度以下，有效地切割最小部分，其中 该角度由读取器读取的线性切口部分的方向和通过单个皮肤扩张器组展开皮肤的方向形成。

公开(公告)号：US20100268259A1

公开(公告)日：2010-10-21

申请号：US12827472

申请日：2010-06-30

申请人： Akio Oki ,  Hiroaki Oka

发明人： Akio Oki ,  Hiroaki Oka

IPC分类号： A61B17/34

CPC分类号： A61B5/15186 ,  A61B5/150022 ,  A61B5/150068 ,  A61B5/150335 ,  A61B5/150389 ,  A61B5/150427 ,  A61B5/150442 ,  A61B5/150503 ,  A61B5/150541 ,  A61B5/150618 ,  A61B5/150717 ,  A61B5/15113 ,  A61B5/15117 ,  A61B5/1519 ,  A61B5/157 ,  A61B5/411

摘要： Provided are skin incision instrument to efficiently incise minimal portions and a method for incising skin with the skin incision instrument. The skin incision instrument according to the present invention comprises a holder, a needle, a needle drive unit, two or more skin expander sets, and a reader, wherein the skin expander sets comprise a first skin expander and a second skin expander; the first skin expander and the second skin expander are capable of expanding skin at both sides of the linear incision portion away from the linear incision portion and expanding the linear incision portion in a direction to expand the linear incision portion; and, minimal portions are efficiently incised by selecting a single skin expander set from two or more of the skin expander set based on the direction of the linear incision portion, such that an angle is adjusted to 45 degrees or more and 90 degrees or less wherein the angle is formed by the direction of the linear incision portion read by the reader and the direction to expand the skin by the single skin expander set.

摘要翻译： 提供有效切割最小部分的皮肤切割仪器和用皮肤切开仪器切割皮肤的方法。 根据本发明的皮肤切开仪器包括保持器，针头，针驱动单元，两个或更多个皮肤膨胀器组和读取器，其中所述皮肤膨胀器组包括第一皮肤膨胀器和第二皮肤膨胀器; 第一皮肤扩张器和第二皮肤扩张器能够使直线切口部分的两侧的皮肤远离线性切口部分，并使线性切口部分沿着扩大线性切口部分的方向膨胀; 并且通过基于线性切口部分的方向从两个或更多个皮肤扩张器组中选择单个皮肤扩张器组，使得角度被调节到45度以上且90度以下，有效地切割最小部分，其中 该角度由读取器读取的线性切口部分的方向和通过单个皮肤扩张器组展开皮肤的方向形成。

公开(公告)号：US20140054170A1

公开(公告)日：2014-02-27

申请号：US14113697

申请日：2012-02-17

申请人： Norihito Tsukahara ,  Keiko Ikuta ,  Akio Oki

发明人： Norihito Tsukahara ,  Keiko Ikuta ,  Akio Oki

IPC分类号： G01N27/327

CPC分类号： G01N27/327 ,  G01N27/40 ,  G01N33/5438 ,  G01N33/92

摘要： A biosensor device which is possible to be downsized and potable, is free of the problem of solution outflow, and enables detection accurately and stably is realized. A biosensor device (30) having an artificial lipid membrane is provided with an artificial lipid membrane (1) having first and second membrane surfaces (1a, 1b) which are located opposite each other, a first chamber (11a) for encapsulating a first electrolyte solution (2a) in contact with the first membrane surface (1a) of the artificial lipid membrane, a second chamber (11b) for encapsulating a second electrolyte solution (2b) in contact with the second membrane surface (1b) of the artificial lipid membrane, first electrodes (3a, 3a′) for contacting the first electrolyte solution (2a) in the first chamber (11a), and second electrodes (3b, 3b′) for contacting the second electrolyte solution (2b) in the second chamber (11b), wherein the second chamber (11b) has gas permeability at its wall portion (10) for encapsulating the second electrolyte solution (2b) in at least a wall thickness direction.

摘要翻译： 可以小型化并且可饮用的生物传感器装置没有溶液流出的问题，能够准确，稳定地进行检测。 具有人造脂膜的生物传感器装置（30）设置有具有彼此相对定位的第一和第二膜表面（1a，1b）的人造脂膜（1），用于封装第一电解质 与人造脂膜的第一膜表面（1a）接触的溶液（2a），用于封装与人造脂膜的第二膜表面（1b）接触的第二电解质溶液（2b）的第二室（11b） ，用于与第一室（11a）中的第一电解液（2a）接触的第一电极（3a，3a'）和用于接触第二室（11b）中的第二电解液（2b）的第二电极 ），其中所述第二室（11b）在其壁部（10）处具有气体渗透性，用于将所述第二电解质溶液（2b）封装在至少壁厚方向上。