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公开(公告)号：US20090305409A1
公开(公告)日：2009-12-10
申请号：US11887030
申请日：2006-03-24
IPC分类号： C12N5/00
摘要： Disclosed is a non-viral vector capable of delivering a given substance into the nucleus of a target cell effectively even when the target cells is a undividable cell such as a dendritic cell. A bilamellar liposome having a first lipid membrane and a second lipid membrane successively from the outside, the first lipid membrane having a membrane-fusing ability and the second lipid membrane having on its surface a nuclear transport peptide.
摘要翻译： 公开了即使当靶细胞是不可分割的细胞例如树突状细胞时，能够有效地将给定物质有效地递送到靶细胞的细胞核中的非病毒载体。 具有从外部连续地具有第一脂质膜和第二脂质膜的第二脂质膜，所述第一脂质膜具有膜融合能力，所述第二脂质膜在其表面上具有核转运肽。
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2.发明申请AGENT FOR ENHANCING THE RESISTANCE OF LIPOSOME AGAINST BIOLOGICAL COMPONENT, AND LIPOSOME MODIFIED WITH THE AGENT 审中-公开用于增强生物组分的脂质体抗性的试剂和用该试剂修饰的脂质体公开(公告)号：US20100104623A1
公开(公告)日：2010-04-29
申请号：US12449817
申请日：2008-02-27
申请人： Hideyoshi Harashima , Kentaro Kogure , Hidetaka Akita , Akitada Iwasa , Yoshio Nakamura , Shiroh Futaki
发明人： Hideyoshi Harashima , Kentaro Kogure , Hidetaka Akita , Akitada Iwasa , Yoshio Nakamura , Shiroh Futaki
IPC分类号： A61K9/127
摘要： It is intended to provide a positively charged liposome, particularly having a polyarginine peptide on a surface thereof, which is capable of increasing the resistance to a negatively charged biological component such as a protein in the blood and maintaining a high ability to deliver a substance even in the blood. An agent for enhancing the resistance of liposome against biological component comprising a peptide having at least one of the following characteristics (a) and (b) as an active ingredient: (a) A peptide comprising an amino acid sequence represented by SEQ ID No: 1 or 2; and (b) A peptide comprising an amino acid sequence represented by SEQ ID No: 1 or 2, in which one or more amino acids are deleted, substituted or added, and having an activity for promoting lipid membrane fusion under acidic condition. An agent for enhancing the resistance of liposome against biological component in this invention, being included in a liposome, is capable of increasing the resistance to a negatively charged biological component such as a protein in the blood and maintaining a high ability to deliver a substance even in the blood.
摘要翻译： 旨在提供带正电荷的脂质体，特别是在其表面具有聚精氨酸肽，其能够增加对带负电的生物成分如血液中的蛋白质的抗性，并且保持高的物质输送能力 在血中 用于增强脂质体对生物成分的抗性的试剂，其包含具有以下特征（a）和（b）中的至少一个作为活性成分的肽：（a）包含SEQ ID No： 1或2; 和（b）包含SEQ ID No：1或2所示的氨基酸序列的肽，其中一个或多个氨基酸缺失，取代或添加，并且具有在酸性条件下促进脂质膜融合的活性。 包含在脂质体中的用于增强本发明中脂质体对抗生物成分的抗性的药剂能够增加对带负电的生物成分如血液中的蛋白质的抗性，并保持高的物质输送能力 在血中
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公开(公告)号：US20130122054A1
公开(公告)日：2013-05-16
申请号：US13641812
申请日：2011-04-20
申请人： Hideyoshi Harashima , Hidetaka Akita , Mohammad Shaheen Sharif , Takashi Nakamura , Soichiro Ishii , Shiroh Futaki
发明人： Hideyoshi Harashima , Hidetaka Akita , Mohammad Shaheen Sharif , Takashi Nakamura , Soichiro Ishii , Shiroh Futaki
IPC分类号： A61K47/42
CPC分类号： A61K9/1271 , A61K9/127 , A61K9/1272 , A61K31/711 , A61K39/39 , A61K47/50 , A61K47/62 , A61K47/6911 , A61K48/0041 , A61K2039/5154 , A61K2039/55555 , C12N15/00 , C12N15/88 , C12N2810/40
摘要： A lipid membrane structure for delivering a substance into a nucleus of a cell, wherein lipid membrane is modified with (a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and/or (b) a polypeptide consisting of an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, but including deletion and/or substitution and/or insertion of one or several amino acid residues, and having an activity of promoting migration of the lipid membrane structure into a nucleus of a cell, which can efficiently deliver a nucleic acid into a nucleus of an immunocyte such as dendritic cell.
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公开(公告)号：US08981044B2
公开(公告)日：2015-03-17
申请号：US13641812
申请日：2011-04-20
申请人： Hideyoshi Harashima , Hidetaka Akita , Mohammad Shaheen Sharif , Takashi Nakamura , Soichiro Ishii , Shiroh Futaki
发明人： Hideyoshi Harashima , Hidetaka Akita , Mohammad Shaheen Sharif , Takashi Nakamura , Soichiro Ishii , Shiroh Futaki
IPC分类号： C07K14/00 , A61K47/42 , A61K9/127 , A61K31/711 , A61K39/39 , A61K47/48 , A61K48/00 , C12N15/00 , C12N15/88 , A61K39/00
CPC分类号： A61K9/1271 , A61K9/127 , A61K9/1272 , A61K31/711 , A61K39/39 , A61K47/50 , A61K47/62 , A61K47/6911 , A61K48/0041 , A61K2039/5154 , A61K2039/55555 , C12N15/00 , C12N15/88 , C12N2810/40
摘要： A lipid membrane structure for delivering a substance into a nucleus of a cell, wherein lipid membrane is modified with (a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and/or (b) a polypeptide consisting of an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, but including deletion and/or substitution and/or insertion of one or several amino acid residues, and having an activity of promoting migration of the lipid membrane structure into a nucleus of a cell, which can efficiently deliver a nucleic acid into a nucleus of an immunocyte such as dendritic cell.
摘要翻译： 一种用于将物质递送到细胞核的脂质膜结构，其中脂质膜用（a）包含SEQ ID NO：1的氨基酸序列的多肽和/或（b）由氨基酸组成的多肽 酸序列，其包含SEQ ID NO：1的氨基酸序列，但包括缺失和/或取代和/或插入一个或几个氨基酸残基，并具有促进脂质膜结构迁移到细胞核中的活性 细胞，其可以有效地将核酸递送到免疫细胞如树突状细胞的细胞核中。
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5.发明授权公开(公告)号：US08946380B2
公开(公告)日：2015-02-03
申请号：US11652865
申请日：2007-01-12
IPC分类号： A61K38/28 , C07K1/00 , A61K9/127 , C07K5/00 , C07K19/00 , C07K17/00 , C07K14/00 , C07K7/00 , C07K16/00
CPC分类号： A61K9/127 , A61K38/28 , C07K1/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K16/00 , C07K17/00 , C07K19/00
摘要： With the aim of providing a liposome, having a hydrophilic polymer introduced into the outer surface of the liposome membrane, which is a liposome capable of allowing the liposome-entrapped substance to escape from the endosome and be released into the cytoplasm, a liposome membrane component bound to the peptide shown by (a) or (b) below and a liposome membrane component bound to one end of a hydrophilic polymer the other end of which is bound to the peptide shown by (a) or (b) below are included in the liposome: (a) a peptide comprising the amino acid sequence of SEQ ID NO:1; (b) a peptide comprising the amino acid sequence of SEQ ID NO:1 with 1 or more amino acids deleted, replaced or added therein, and capable of fusing lipid membranes with one another under acidic conditions.
摘要翻译： 为了提供脂质体，其具有引入脂质体膜的外表面的亲水性聚合物，脂质体膜是能够使脂质体包裹的物质从内体中逸出并释放到细胞质中的脂质体，脂质体膜组分 与下文（a）或（b）所示的肽结合，并且与另一端与下文（a）或（b）所示的肽结合的亲水性聚合物的一端结合的脂质体膜组分包括在 脂质体：（a）包含SEQ ID NO：1的氨基酸序列的肽; （b）包含SEQ ID NO：1的氨基酸序列的肽，其中1个或多个缺失，替换或添加的氨基酸，并且能够在酸性条件下彼此融合脂质膜。
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6.发明申请公开(公告)号：US20070299244A1
公开(公告)日：2007-12-27
申请号：US11652865
申请日：2007-01-12
CPC分类号： A61K9/127 , A61K38/28 , C07K1/00 , C07K5/00 , C07K7/00 , C07K14/00 , C07K16/00 , C07K17/00 , C07K19/00
摘要： With the aim of providing a liposome, having a hydrophilic polymer introduced into the outer surface of the liposome membrane, which is a liposome capable of allowing the liposome-entrapped substance to escape from the endosome and be released into the cytoplasm, a liposome membrane component bound to the peptide shown by (a) or (b) below and a liposome membrane component bound to one end of a hydrophilic polymer the other end of which is bound to the peptide shown by (a) or (b) below are included in the liposome: (a) a peptide comprising the amino acid sequence of SEQ ID NO:1; (b) a peptide comprising the amino acid sequence of SEQ ID NO:1 with 1 or more amino acids deleted, replaced or added therein, and capable of fusing lipid membranes with one another under acidic conditions.
摘要翻译： 为了提供脂质体，其具有引入脂质体膜的外表面的亲水性聚合物，脂质体膜是能够使脂质体包裹的物质从内体中逸出并释放到细胞质中的脂质体，脂质体膜组分 与下文（a）或（b）所示的肽结合，并且与另一端与下文（a）或（b）所示的肽结合的亲水性聚合物的一端结合的脂质体膜组分包括在 脂质体：（a）包含SEQ ID NO：1的氨基酸序列的肽; （b）包含SEQ ID NO：1的氨基酸序列的肽，其中1个或多个缺失，替换或添加的氨基酸，并且能够在酸性条件下彼此融合脂质膜。
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公开(公告)号：US08592210B2
公开(公告)日：2013-11-26
申请号：US11396081
申请日：2006-03-31
CPC分类号： C12N15/88 , A61K9/1273 , A61K47/34 , A61K47/645 , A61K47/6911 , C07K1/1077
摘要： With the object of providing a liposome having cellular and nuclear entry ability, to achieve this object, a liposome is provided having on its surface a peptide comprising multiple consecutive arginine residues, and specifically a liposome is provided wherein the peptide is modified with a hydrophobic group or hydrophobic compound and the hydrophobic group or hydrophobic compound is inserted into a lipid bilayer so that the peptide is exposed on the surface of the bilayer.
摘要翻译： 为了提供具有细胞和核进入能力的脂质体，为了达到这个目的，提供了一种脂质体，其表面上含有多个连续的精氨酸残基的肽，特别是提供一种脂质体，其中肽用疏水基团修饰 或疏水性化合物，并且将疏水基团或疏水性化合物插入脂质双层中，使得该肽暴露在双层的表面上。
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公开(公告)号：US20070059353A1
公开(公告)日：2007-03-15
申请号：US11396081
申请日：2006-03-31
CPC分类号： C12N15/88 , A61K9/1273 , A61K47/34 , A61K47/645 , A61K47/6911 , C07K1/1077
摘要： With the object of providing a liposome having cellular and nuclear entry ability, to achieve this object, a liposome is provided having on its surface a peptide comprising multiple consecutive arginine residues, and specifically a liposome is provided wherein the peptide is modified with a hydrophobic group or hydrophobic compound and the hydrophobic group or hydrophobic compound is inserted into a lipid bilayer so that the peptide is exposed on the surface of the bilayer.
摘要翻译： 为了提供具有细胞和核进入能力的脂质体，为了达到这个目的，提供了一种脂质体，其表面上含有多个连续的精氨酸残基的肽，特别是提供一种脂质体，其中肽用疏水基团修饰 或疏水性化合物，并且将疏水基团或疏水性化合物插入脂质双层中，使得该肽暴露在双层的表面上。
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公开(公告)号：US20100136099A1
公开(公告)日：2010-06-03
申请号：US12449670
申请日：2008-02-26
申请人： Hideyoshi Harashima , Kentaro Kogure , Takashi Nakamura , Rumiko Moriguchi , Kohei Ono , Homhuan Atthachai
发明人： Hideyoshi Harashima , Kentaro Kogure , Takashi Nakamura , Rumiko Moriguchi , Kohei Ono , Homhuan Atthachai
CPC分类号： A61K9/1272 , A61K39/0011 , A61K39/385 , A61K39/39 , A61K2039/55516 , A61K2039/55555
摘要： Disclosed is a liposome which includes an antigenic substance therein and has, on its surface, a peptide containing several contiguous arginine residues. The liposome enables an antigen-presenting cell to achieve the selective antigen presentation through an MHC Class-I molecule, and can induce a cell-mediated immunity specifically. The liposome can also induce the antigen presentation by a matured dendritic cell.
摘要翻译： 公开了一种在其中包含抗原物质的脂质体，其表面上含有几个连续的精氨酸残基的肽。 脂质体使得抗原呈递细胞能够通过MHC I类分子实现选择性抗原呈递，并且可以特异性诱导细胞介导的免疫。 脂质体也可以通过成熟的树突状细胞诱导抗原呈递。
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10.发明申请PEPTIDES IMPARTING CELL PERMEABILITY TO LIPID MEMBRANE STRUCTURE AND/OR ENHANCING CELL PERMEABILITY OF LIPID MEMBRANE STRUCTURE, AND LIPID MEMBRANE STRUCTURE COMPRISING LIPID BOUND TO SUCH PEPTIDE AS CONSTITUENT LIPID AND HAVING CELL PERMEABILITY OR SHOWING ENHANCED CELL PERMEABILITY 有权PEPTIDES将脂质膜结构和/或增强脂质膜结构的细胞渗透性和/或增强脂质膜结构的细胞渗透性的脂质渗透性，以及将包含脂质的脂质膜结合到这些肽作为组织脂质并具有细胞渗透性或显示增强的细胞渗透性公开(公告)号：US20120309937A1
公开(公告)日：2012-12-06
申请号：US13515970
申请日：2010-12-14
CPC分类号： C07K7/06 , A61K9/1271 , A61K38/00 , A61K47/60 , A61K47/62 , A61K47/6911
摘要： Provided are peptides imparting cell permeability to a lipid membrane structure and/or enhancing the cell permeability of a lipid membrane structure, and a lipid membrane structure which comprises, as a constituent lipid, a lipid bound to such a peptide and has cell permeability or shows enhanced cell permeability. The amino acid sequences of the peptides imparting cell permeability to a lipid membrane structure and/or enhancing the cell permeability of a lipid membrane structure are represented by: LX1X2X1X1X1L, LLX2X1X1X1L and LX1X2X1X1L (wherein L represents a leucine residue; X1 represents a polar amino acid residue; and X2 represents a polar, non-charged and branched chain amino acid residue).
摘要翻译： 提供赋予脂质膜结构的细胞渗透性和/或增强脂质膜结构的细胞渗透性的肽，以及脂质膜结构，其包含作为构成脂质的与这种肽结合的脂质并具有细胞渗透性或显示 增强细胞渗透性。 赋予细胞对脂质膜结构的渗透性和/或增强脂质膜结构的细胞渗透性的肽的氨基酸序列由以下表示：LX1X2X1X1X1L，LLX2X1X1X1L和LX1X2X1X1L（其中L表示亮氨酸残基; X1表示极性氨基酸 残基; X2表示极性，非带电荷和支链氨基酸残基）。
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