公开(公告)号：US06708730B2

公开(公告)日：2004-03-23

申请号：US10101595

申请日：2002-03-21

申请人： Hiroshi Chikuma ,  Takahiro Nakakomi ,  Hirokazu Yaezawa ,  Yoshinobu Okuno ,  Katsumi Nakamura

发明人： Hiroshi Chikuma ,  Takahiro Nakakomi ,  Hirokazu Yaezawa ,  Yoshinobu Okuno ,  Katsumi Nakamura

IPC分类号： F16L4108

CPC分类号： F28F9/0248 ,  B23K33/00 ,  B23K33/006 ,  F16L37/008 ,  F16L41/08 ,  F28F9/0246 ,  F28F9/0258 ,  F28F9/182 ,  F28F2275/146

摘要： A temporary fixing structure includes recesses formed with a mounting hole of a first tubular body to face each other wherein the diameter of the mounting hole extending along the recesses is smaller than that of a flange, and protrusions arranged at the head of an end portion of a second tubular body wherein a spacing between the protrusion and the flange is substantially equal to the thickness of a mounting surface of the first tubular body and wherein the protrusions are arranged inside the fist tubular body through the recesses. The second tubular body is turned with respect to the first tubular body to bring a flat portion of the flange into close contact with the mounting surface of the first tubular body.

摘要翻译： 临时固定结构包括形成有第一管状体的安装孔以相对于彼此面对的凹部，其中沿着凹部延伸的安装孔的直径小于凸缘的直径，并且突出部设置在凸缘的端部的头部 第二管状体，其中所述突起和所述凸缘之间的间隔基本上等于所述第一管状体的安装表面的厚度，并且其中所述突起通过所述凹部布置在所述第一管状体的内部。 第二管状体相对于第一管状体转动以使凸缘的平坦部分与第一管状体的安装表面紧密接触。

公开(公告)号：US06874230B2

公开(公告)日：2005-04-05

申请号：US10029396

申请日：2001-12-28

申请人： Hiroshi Chikuma ,  Yoshinobu Okuno ,  Takahiro Nakakomi

发明人： Hiroshi Chikuma ,  Yoshinobu Okuno ,  Takahiro Nakakomi

IPC分类号： B60H1/32 ,  B21D53/08 ,  F28D1/04 ,  F28D7/16 ,  F28F1/30 ,  F28F9/013 ,  B21D53/06 ,  B23P15/26

CPC分类号： F28F9/001 ,  B21D53/085 ,  B23P15/26 ,  F28D1/0435 ,  Y10T29/4935 ,  Y10T29/49373 ,  Y10T29/49377 ,  Y10T29/49378 ,  Y10T29/49895 ,  Y10T29/49901 ,  Y10T29/49998 ,  Y10T29/53117 ,  Y10T29/53122

摘要： A method of manufacturing a heat exchanger includes processes of: arranging tubes at a predetermined pitch on a set base; inserting fins into spaces each defined between the tubes; compressing the tubes and the fins in the direction of arrangement thereof; mounting hollow headers to longitudinal ends of the tubes, each header having slits engaged with a corresponding end of the tubes; and attaching end covers to both ends of the headers, each cover closing holes which open at the corresponding end of the headers.

摘要翻译： 制造热交换器的方法包括以下步骤：将预定间距的管排列在固定基座上; 将翅片插入每个限定在管之间的空间中; 在其布置方向上压缩管和翅片; 将空心集管安装到管的纵向端部，每个集管具有与管的相应端部接合的狭缝; 并且将端盖附接到集管的两端，每个盖封闭孔，其在集管的相应端部开口。

公开(公告)号：US06651334B2

公开(公告)日：2003-11-25

申请号：US10176408

申请日：2002-06-21

申请人： Hiroshi Chikuma ,  Yoshinobu Okuno ,  Takahiro Nakakomi

发明人： Hiroshi Chikuma ,  Yoshinobu Okuno ,  Takahiro Nakakomi

IPC分类号： F28F902

CPC分类号： F28F9/0243 ,  F28F9/0212 ,  Y10T29/49389

摘要： A tank of a condenser for an air conditioning system of an automotive vehicle. The tank comprises a cylindrical hollow tank main body which is formed with an arcuate cutout formed through a wall of the tank main body. A partition plate includes generally semicircular large and small diameter sections which are integral with each other to be formed into a generally disc-shape. Two projections are radially outwardly protrude respectively from opposite end portions of the generally semicircular large diameter section. During production of the tank, the partition plate is temporarily fixed to the tank main body by riveting the two projections in a state in which the partition plate has been inserted through the cutout of the tank main body, and the partition plate is brazed to the tank main body in a state in which the plate has been temporarily fixed.

公开(公告)号：US08975378B2

公开(公告)日：2015-03-10

申请号：US13141998

申请日：2009-12-24

申请人： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Yoshinobu Okuno ,  Mikihiro Yunoki ,  Shoji Ideno ,  Masatoshi Oshita ,  Motoki Kuhara ,  Masatoshi Momota

发明人： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Yoshinobu Okuno ,  Mikihiro Yunoki ,  Shoji Ideno ,  Masatoshi Oshita ,  Motoki Kuhara ,  Masatoshi Momota

IPC分类号： C07K16/10 ,  C12N15/13 ,  A61K39/00

CPC分类号： C07K16/1018 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76

摘要： Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.

摘要翻译： 本发明提供对人流感病毒具有中和活性的人抗体。 更具体地，提供了识别人流感A型病毒亚型H3N2或人类流感B型病毒中的高度保守区域并具有针对病毒的中和活性的人抗体。 人抗体是人抗流感病毒抗体，其对人甲型流感病毒亚型H3N2具有中和活性，并与人甲型流感病毒亚型H3N2的血凝素HA1区域结合，或者具有中和活性 人流感B病毒，并且作为编码抗体可变区的DNA的碱基序列，包含SEQ ID NO：5〜12中任一项所述的序列。

公开(公告)号：US20110319600A1

公开(公告)日：2011-12-29

申请号：US13141998

申请日：2009-12-24

申请人： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Yoshinobu Okuno ,  Mikihiro Yunoki ,  Shoji Ideno ,  Masatoshi Oshita ,  Motoki Kuhara ,  Masatoshi Momota

发明人： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Yoshinobu Okuno ,  Mikihiro Yunoki ,  Shoji Ideno ,  Masatoshi Oshita ,  Motoki Kuhara ,  Masatoshi Momota

IPC分类号： C07K16/10 ,  C12N15/13

CPC分类号： C07K16/1018 ,  A61K2039/505 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76

摘要： Provided is a human antibody having a neutralization activity against a human influenza virus. More specifically, provided is a human antibody which recognizes a highly conserved region in a human influenza A virus subtype H3N2 or a human influenza B virus and has a neutralization activity against the virus. The human antibody is a human anti-human influenza virus antibody, which has a neutralization activity against a human influenza A virus subtype H3N2 and binds to a hemagglutinin HA1 region of the human influenza A virus subtype H3N2, or which has a neutralization activity against a human influenza B virus, and includes, as a base sequence of a DNA encoding a variable region of the antibody, a sequence set forth in any one of SEQ ID NOS: 5 to 12.

摘要翻译： 本发明提供对人流感病毒具有中和活性的人抗体。 更具体地，提供了识别人流感A型病毒亚型H3N2或人类流感B型病毒中的高度保守区域并具有针对病毒的中和活性的人抗体。 人抗体是人抗流感病毒抗体，其对人甲型流感病毒亚型H3N2具有中和活性，并与人甲型流感病毒亚型H3N2的血凝素HA1区域结合，或者具有中和活性 人流感B病毒，并且作为编码抗体可变区的DNA的碱基序列，包含SEQ ID NO：5〜12中任一项所述的序列。

公开(公告)号：US20120156242A1

公开(公告)日：2012-06-21

申请号：US13375661

申请日：2010-06-16

申请人： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Akifumi Yamashita ,  Norihito Kawashita ,  Mikihiro Yunoki ,  Yoshinobu Okuno ,  Shoji Ideno ,  Motoki Kuhara

发明人： Kazuyoshi Ikuta ,  Ritsuko Koketsu ,  Akifumi Yamashita ,  Norihito Kawashita ,  Mikihiro Yunoki ,  Yoshinobu Okuno ,  Shoji Ideno ,  Motoki Kuhara

IPC分类号： A61K39/145 ,  C07K7/06 ,  C07K7/08 ,  C07K14/11 ,  A61P31/16 ,  G01N33/566 ,  G01N33/569 ,  C07K16/10 ,  A61P37/04 ,  C07K2/00 ,  C07H21/00

CPC分类号： C07K16/1018 ,  A61K39/00 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K14/005 ,  C07K2317/21 ,  C07K2317/34 ,  C07K2317/76 ,  C12N2760/16122

摘要： Antigenic peptides are provided that can be used to induce global neutralizing antibodies, or antibodies reactive against a wide range of influenza A virus strains. The antigenic peptide can correspond to SEQ ID NO: 34 (EKEVLVLWG), SEQ ID NO: 2 (KFDKLYIWG), SEQ ID NO: 71(QEDLLVLWG), SEQ ID NO: 51 (EGRINYYWTLLEP), SEQ ID NO: 3 (PSRISIYWTIVKP), and/or SEQ ID NO: 82 (SGRMEFFWTILKP).

摘要翻译： 提供了可用于诱导全球中和抗体或针对大范围甲型流感病毒株反应的抗体的抗原肽。 抗原肽可以对应于SEQ ID NO：34（EKEVLVLWG），SEQ ID NO：2（KFDKLYIWG），SEQ ID NO：71（QEDLLVLWG），SEQ ID NO：51（EGRINYYWTLLEP），SEQ ID NO：3（PSRISIYWTIVKP） ，和/或SEQ ID NO：82（SGRMEFFWTILKP）。

公开(公告)号：US06720409B2

公开(公告)日：2004-04-13

申请号：US09918568

申请日：2001-08-01

申请人： Yoshinobu Okuno ,  Yuji Isegawa ,  Fuyoko Sasao ,  Shigeharu Ueda

发明人： Yoshinobu Okuno ,  Yuji Isegawa ,  Fuyoko Sasao ,  Shigeharu Ueda

IPC分类号： C07H2102

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K16/1018 ,  C12N2760/16122

摘要： [Object] To provide an anti-human influenza virus antibody and an immunogenic artificial polypeptide. [Constitution] An anti-human influenza virus antibody which recognizes the stem regions of haemagglutinin molecules of the H1N1 and H2N2 subtypes and has a neutralization activity but does not recognize the stem region of the H3N2 subtype and has no neutralization activity. An immunogenic artificial polypeptide having an antigenicity substantially same as the stem regions of haemagglutinin molecules. An immunogenic artificial polypeptide having an antigenicity substantially same as the stem regions of haemagglutinin molecule and lacking the globular head region of haemagglutinin molecules. [Effect] This antibody is useful in the diagnosis and treatment of influenza A virus, while these polypeptides are useful as a vaccine.

摘要翻译： 提供抗人流感病毒抗体和免疫原性人工多肽。[构成]一种抗人流感病毒抗体，其识别H1N1和H2N2亚型的血细胞凝集素分子的茎区，并具有中和活性，但不 识别H3N2亚型的干细胞区域，没有中和活性。 具有与血细胞凝集素分子的茎区域基本相同的抗原性的免疫原性人工多肽。 具有与血细胞凝集素分子的茎区域基本相同的抗原性的免疫原性人工多肽，并且缺乏血细胞凝集素分子的球形头部区域。[作用]该抗体可用于甲型流感病毒的诊断和治疗，而这些多肽可用作 疫苗。

公开(公告)号：US06337070B1

公开(公告)日：2002-01-08

申请号：US09004422

申请日：1998-01-08

申请人： Yoshinobu Okuno ,  Yuji Isegawa ,  Fuyoko Sasao ,  Shigeharu Ueda

发明人： Yoshinobu Okuno ,  Yuji Isegawa ,  Fuyoko Sasao ,  Shigeharu Ueda

IPC分类号： A61K3912

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K16/1018 ,  C12N2760/16122

摘要： The invention provides an anti-human influenza virus antibody which recognizes the stem regions of haemagglutinin molecules of the H1N1 and H2N2 subtypes and has a neutralization activity but does not recognize the stem region of the H3N2 subtype and has no neutralization activity. Also provided is an immunogenic artificial polypeptide having an antigenicity substantially same as the stem region of haemagglutinin molecules, particularly lacking the globular head region of haemagglutinin molecules. This antibody is useful in the diagnosis and treatment of influenza A virus, while the polypeptides are useful as a vaccine.

摘要翻译： 本发明提供一种抗人流感病毒抗体，其识别H1N1和H2N2亚型的血细胞凝集素分子的茎区，并具有中和活性，但不识别H3N2亚型的茎区并且没有中和活性。 还提供了具有与血细胞凝集素分子的茎区域基本上相同的抗原性的免疫原性人造多肽，特别是缺乏血细胞凝集素分子的球形头部区域。该抗体可用于甲型流感病毒的诊断和治疗，而多肽可用作 一种疫苗

公开(公告)号：US5589174A

公开(公告)日：1996-12-31

申请号：US229781

申请日：1994-04-19

申请人： Yoshinobu Okuno ,  Yuji Isegawa ,  Fuyoko Sasao ,  Shigeharu Ueda

发明人： Yoshinobu Okuno ,  Yuji Isegawa ,  Fuyoko Sasao ,  Shigeharu Ueda

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/11 ,  C07K16/10 ,  A61K39/395 ,  C07K16/08 ,  C12N5/20 ,  C12N15/06

CPC分类号： C07K14/005 ,  C07K16/1018 ,  A61K38/00 ,  A61K39/00 ,  C12N2760/16122 ,  C12N2760/16222

摘要： An anti-human influenza virus antibody is provided which recognizes the stem regions of haemagglutinin molecules of the H1N1 and H2N2 subtypes and has a neutralization activity, but does not recognize the stem region of the H3N2 subtype and has no neutralization activity. Also provided is an immunogenic artificial polypeptide having an antigenicity substantially the same as the stem regions of haemagglutinin molecules. Provided further is an immunogenic artificial polypeptide having an antigenicity substantially the same as the stem regions of haemagglutinin molecule and lacking the globular head region of haemagglutinin molecules. The antibody is useful in the diagnosis and treatment of influenza A virus, while the polypeptides are useful as a vaccine.

摘要翻译： 提供抗人流感病毒抗体，其识别H1N1和H2N2亚型的血细胞凝集素分子的茎区，并具有中和活性，但不识别H3N2亚型的茎区并且没有中和活性。 还提供了具有与血细胞凝集素分子的茎区域基本相同的抗原性的免疫原性人造多肽。 还提供了具有与血细胞凝集素分子的茎区域基本相同的抗原性并且缺少血细胞凝集素分子的球形头部区域的免疫原性人造多肽。 该抗体可用于甲型流感病毒的诊断和治疗，而多肽可用作疫苗。

公开(公告)号：US08101553B1

公开(公告)日：2012-01-24

申请号：US10204773

申请日：2001-02-22

申请人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Yoshitaka Iba ,  Kazuhiko Morino ,  Midori Shinohara ,  Motohide Takahashi ,  Yoshinobu Okuno ,  Kimiyasu Shiraki

发明人： Yoshikazu Kurosawa ,  Yasushi Akahori ,  Yoshitaka Iba ,  Kazuhiko Morino ,  Midori Shinohara ,  Motohide Takahashi ,  Yoshinobu Okuno ,  Kimiyasu Shiraki

IPC分类号： C40B50/06

CPC分类号： C12N15/10 ,  C07K16/00 ,  C07K2317/50 ,  C07K2317/56

摘要： An antibody library is prepared by selecting a light chain variable region capable of binding to the variable region of heavy chain to reproduce an active conformation and using the same. Because of being capable of maintaining the diversity of the heavy chain variable region at a high ratio in vitro, the antibody library of the present invention is expected as enabling the acquisition of antibodies with various binding activities.

摘要翻译： 通过选择能够结合重链可变区的轻链可变区以再现活性构象并使用它们来制备抗体文库。 由于能够在体外以高比例保持重链可变区的多样性，本发明的抗体文库预期能够获得具有各种结合活性的抗体。