公开(公告)号：US20080153142A1

公开(公告)日：2008-06-26

申请号：US11660957

申请日：2005-08-18

申请人： Hiroshi Kawashima ,  Eiji Sakuradani ,  Jun Ogawa ,  Sakayu Shimizu

发明人： Hiroshi Kawashima ,  Eiji Sakuradani ,  Jun Ogawa ,  Sakayu Shimizu

IPC分类号： C12P7/64 ,  C07C57/00 ,  C12N1/14

CPC分类号： A23K20/158 ,  A23D9/00 ,  A23L2/52 ,  A23L33/115 ,  C11B1/10 ,  C12P7/6472

摘要： The invention provides a process, for producing fats or oils in which the rate of triglycerides constituted by the same polyunsaturated fatty acids linking to the three hydroxyl groups of glycerol is 20% or more by weight for the total triglycerides, which comprises culturing a microorganism capable of producing said fats or oils and if required recovering said fats or oils; fats or oils produced by this process; and a process for producing said fats or oils.

摘要翻译： 本发明提供了一种用于生产脂肪或油的方法，其中由与甘油的三个羟基连接的相同的多不饱和脂肪酸构成的甘油三酯的比例为总甘油三酯的20重量％或更多，其包括培养能够培养的微生物 生产所述脂肪或油，并且如果需要回收所述脂肪或油; 通过这种方法生产的油脂; 以及生产所述脂肪或油的方法。

公开(公告)号：US09051590B2

公开(公告)日：2015-06-09

申请号：US13254512

申请日：2010-03-17

申请人： Misa Ochiai ,  Eiji Sakuradani ,  Sakayu Shimizu ,  Jun Ogawa

发明人： Misa Ochiai ,  Eiji Sakuradani ,  Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P21/06 ,  C12P21/04 ,  C12N15/00 ,  C12P7/64 ,  A23L1/30 ,  C12N9/00

CPC分类号： C12P7/6427 ,  A23L33/115 ,  A23V2002/00 ,  C12N9/93 ,  C12Y604/01002 ,  A23V2250/1882

摘要： The present invention provides a novel acetyl-CoA carboxylase.The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.

摘要翻译： 本发明提供了一种新的乙酰辅酶A羧化酶。 本发明的目的是通过本发明的SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的氨基酸序列来实现的。

公开(公告)号：US20080138874A1

公开(公告)日：2008-06-12

申请号：US10594772

申请日：2005-03-28

申请人： Misa Ochiai ,  Hiroshi Kawashima ,  Sakayu Shimizu ,  Eiji Sakuradani

发明人： Misa Ochiai ,  Hiroshi Kawashima ,  Sakayu Shimizu ,  Eiji Sakuradani

IPC分类号： C12P1/02 ,  C12N15/74 ,  C12N1/00

CPC分类号： C12P7/6472 ,  C12P7/6463 ,  C12R1/645

摘要： A method of breeding a lipid-producing strain belonging to the genus Mortierella. According to the method of breeding a lipid-producing strain which involves an expression-inhibiting step of inhibiting the expression of a specific gene in the lipid-producing strain as described above, a lipid-producing strain belonging to the genus Mortierella can be efficiently and effectively bred.

摘要翻译： 育种属于被孢霉属的脂质生产菌株的方法。 根据如上所述的涉及抑制脂质生产菌株中的特定基因表达的表达抑制步骤的产生脂质的菌株的方法，可以有效地和属于被孢霉属的脂质生产菌株 有效繁殖。

公开(公告)号：US20120264960A1

公开(公告)日：2012-10-18

申请号：US13254512

申请日：2010-03-17

申请人： Misa Ochiai ,  Eiji Sakuradani ,  Sakayu Shimizu ,  Jun Ogawa

发明人： Misa Ochiai ,  Eiji Sakuradani ,  Sakayu Shimizu ,  Jun Ogawa

IPC分类号： C12N9/00 ,  C12N15/63 ,  C12P7/64 ,  C07C57/03 ,  C12N1/15 ,  C12N1/19 ,  C12N5/10 ,  C12P9/00 ,  C12N15/52 ,  C12N1/21

CPC分类号： C12P7/6427 ,  A23L33/115 ,  A23V2002/00 ,  C12N9/93 ,  C12Y604/01002 ,  A23V2250/1882

摘要： The present invention provides a novel acetyl-CoA carboxylase. The object of the present invention is attained by the nucleotide sequence of SEQ ID NO: 1 and the amino acid sequence of SEQ ID NO: 2 of the present invention.

摘要翻译： 本发明提供了一种新的乙酰辅酶A羧化酶。 本发明的目的是通过本发明的SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的氨基酸序列来实现的。

公开(公告)号：US20080269329A1

公开(公告)日：2008-10-30

申请号：US11660859

申请日：2005-08-19

申请人： Shigeaki Fujikawa ,  Eiji Sakuradani ,  Jun Ogawa ,  Sakayu Shimizu

发明人： Shigeaki Fujikawa ,  Eiji Sakuradani ,  Jun Ogawa ,  Sakayu Shimizu

IPC分类号： C12P7/64 ,  A61K31/201 ,  C07C57/00 ,  A61K31/202

CPC分类号： A61K8/375 ,  A23D9/013 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12 ,  A23V2002/00 ,  A61K8/67 ,  A61K8/922 ,  A61K8/99 ,  A61Q19/00 ,  C11B1/10 ,  C11B3/12 ,  C12P7/6472 ,  A23V2250/182 ,  A23V2250/1882

摘要： In a process for the production of diacylglycerol-containing fat/oil where the ratio of diacylglycerol to the total neutral lipid is more than 20% by weight and a polyunsaturated fatty acid is a constituting fatty acid, a process which is characterized in that a microbe being able to produce said fat/oil is incubated and, if desired, said fat/oil is collected.

摘要翻译： 在制备含二酰基甘油的脂肪/油的方法中，其中二酰基甘油与总中性脂质的比例大于20重量％，多不饱和脂肪酸是构成脂肪酸的方法，其特征在于将微生物 能够产生所述脂肪/油的温育，如果需要，收集所述脂肪/油。

公开(公告)号：US07615364B2

公开(公告)日：2009-11-10

申请号：US11660093

申请日：2005-08-19

申请人： Sakayu Shimizu ,  Eiji Sakuradani

发明人： Sakayu Shimizu ,  Eiji Sakuradani

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C07H21/02 ,  C12N5/00 ,  A01H9/00

CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6409

摘要： The present invention provides a polypeptide having a wide substrate specificity and having a ω3 fatty acid desaturation activity, which makes efficiently unsaturated bond at ω3 position, and a polynucleotide coding for the same. By expressing the polypeptide in the organism, mass production of n-3 series PUFAs is enabled. Specifically, the polypeptide having a ω3 fatty acid desaturation activity and consisting of the amino acid sequence represented by SEQ ID NO: 1, a polynucleotide coding for a polypeptide having a ω3 fatty acid desaturation activity that consists of the base sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3, and the like is useful for production of n-3 series fatty acids.

摘要翻译： 本发明提供了具有宽底物特异性并具有ω3脂肪酸去饱和活性的多肽，其在ω3位上有效地形成不饱和键，以及编码该多肽的多核苷酸。 通过在生物体中表达多肽，可以批量生产n-3系PUFA。 具体地说，具有ω3脂肪酸去饱和活性的多肽，由SEQ ID NO：1表示的氨基酸序列组成，该多核苷酸编码具有ω3脂肪酸去饱和活性的多肽，其由SEQ ID NO： ：2或SEQ ID NO：3等可用于生产n-3系列脂肪酸。

公开(公告)号：US20080032335A1

公开(公告)日：2008-02-07

申请号：US11660093

申请日：2005-08-19

申请人： Sakayu Shimizu ,  Eiji Sakuradani

发明人： Sakayu Shimizu ,  Eiji Sakuradani

IPC分类号： C12N15/09 ,  A23L1/30 ,  C07H21/04 ,  C07K14/37 ,  C12N5/10 ,  C12P7/64 ,  C12P21/00 ,  C12N15/63 ,  C07K16/14 ,  C12N1/15

CPC分类号： C12P7/6427 ,  C12N9/0083 ,  C12P7/6409

摘要： The present invention provides a polypeptide having a wide substrate specificity and having a ω3 fatty acid desaturation activity, which makes efficiently unsaturated bond at ω3 position, and a polynucleotide coding for the same. By expressing the polypeptide in the organism, mass production of n-3 series PUFAs is enabled. Specifically, the polypeptide having a ω3 fatty acid desaturation activity and consisting of the amino acid sequence represented by SEQ ID NO: 1, a polynucleotide coding for a polypeptide having a ω3 fatty acid desaturation activity that consists of the base sequence represented by SEQ ID NO: 2 or SEQ ID NO: 3, and the like is useful for production of n-3 series fatty acids.

摘要翻译： 本发明提供了具有宽底物特异性并具有ω3脂肪酸去饱和活性的多肽，其在ω3位上有效地形成不饱和键，以及编码该多肽的多核苷酸。 通过在生物体中表达多肽，可以批量生产n-3系PUFA。 具体地说，具有ω3脂肪酸去饱和活性的多肽，由SEQ ID NO：1表示的氨基酸序列组成，该多核苷酸编码具有ω3脂肪酸去饱和活性的多肽，其由SEQ ID NO： ：2或SEQ ID NO：3等可用于生产n-3系列脂肪酸。

公开(公告)号：US5376541A

公开(公告)日：1994-12-27

申请号：US953044

申请日：1992-09-29

申请人： Hiroshi Kawashima ,  Kengo Akimoto ,  Hideaki Yamada ,  Sakayu Shimizu

发明人： Hiroshi Kawashima ,  Kengo Akimoto ,  Hideaki Yamada ,  Sakayu Shimizu

IPC分类号： C12P7/64 ,  C12R1/645 ,  C12R1/66 ,  C12R1/785 ,  C12R1/80 ,  C12P7/40 ,  C12P7/62

CPC分类号： C12P7/6427 ,  C12P7/6472

摘要： A process for the production of 8,11-eicosadienoic acid or a lipid containing 8,11-eicosadienoic acid comprising the steps of, culturing a microorganism having an ability to produce an omega 9 type polyunsaturated fatty acid in a medium supplemented with a .DELTA.5 desaturase inhibitor, or adding a .DELTA.5 desaturase inhibitor to a medium in which said microorganism has been cultured and further culturing the microorganism to produce 8,11-eicosadienoic acid, or a lipid containing 8,11-eicosadienoic acid, and recovering the 8,11-eicosadienoic acid, or the lipid containing 8,11-eicosadienoic acid.

摘要翻译： 制备8,11-二十碳二烯酸或含有8,11-二十碳二烯酸的脂质的方法，包括以下步骤：在补充有DELTA 5的培养基中培养具有产生ω9型多不饱和脂肪酸的能力的微生物 去饱和酶抑制剂，或向已经培养了所述微生物的培养基中加入DELTA5去饱和酶抑制剂，并进一步培养该微生物以产生8,11-二十碳二烯酸或含有8,11-二十碳二烯酸的脂质， 11-二十碳二烯酸或含有8,11-二十碳二烯酸的脂质。

公开(公告)号：US20070099279A1

公开(公告)日：2007-05-03

申请号：US11606983

申请日：2006-12-01

申请人： Kengo Akimoto ,  Hiroshi Kawashima ,  Sakayu Shimizu

发明人： Kengo Akimoto ,  Hiroshi Kawashima ,  Sakayu Shimizu

IPC分类号： C12P7/64 ,  C12N1/16

CPC分类号： A61K31/202 ,  A23C9/1528 ,  A23C11/04 ,  A23D9/00 ,  A23K20/158 ,  A23L2/52 ,  A23L5/00 ,  A23L31/00 ,  A23L33/12 ,  A23L33/14 ,  A23V2002/00 ,  A61K8/14 ,  A61K8/92 ,  A61K8/925 ,  A61K8/99 ,  A61K31/201 ,  A61Q19/00 ,  C12P7/6472 ,  C12R1/645 ,  A23V2200/224 ,  A23V2250/18 ,  A23V2250/1862 ,  A23V2250/187 ,  A23V2250/1868

摘要： There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.

公开(公告)号：US20070077638A1

公开(公告)日：2007-04-05

申请号：US11607032

申请日：2006-12-01

申请人： Kengo Akimoto ,  Hiroshi Kawashima ,  Sakayu Shimizu

发明人： Kengo Akimoto ,  Hiroshi Kawashima ,  Sakayu Shimizu

IPC分类号： C12P7/64 ,  C12N1/16

CPC分类号： A61K31/202 ,  A23C9/1528 ,  A23C11/04 ,  A23D9/00 ,  A23K20/158 ,  A23L2/52 ,  A23L5/00 ,  A23L31/00 ,  A23L33/12 ,  A23L33/14 ,  A23V2002/00 ,  A61K8/14 ,  A61K8/92 ,  A61K8/925 ,  A61K8/99 ,  A61K31/201 ,  A61Q19/00 ,  C12P7/6472 ,  C12R1/645 ,  A23V2200/224 ,  A23V2250/18 ,  A23V2250/1862 ,  A23V2250/187 ,  A23V2250/1868

摘要： There are provided microorganisms having a property of producing a lipid containing unsaturated fatty acids as constituent fatty acids and extracellularly secreting the produced lipid encapsulated in lipid vesicles, methods of screening said microorganisms, as well as methods of efficiently producing a fatty acid-containing lipid using said microorganisms. Furthermore, there are provided lipid vesicles encapsulating a lipid containing unsaturated fatty acids, and foods, cosmetics, and animal feeds comprising said lipid vesicles added thereto. Artificially treated microorganisms or microorganisms collected from nature are grown on a solid medium, and microbial strains that form lipid vesicles at the periphery of the colonies and/or microbial strains that, when cultured in a transparent liquid medium, make the culture liquid cloudy are selected. The microorganisms obtained are cultured, lipid-containing lipid vesicles secreted in the culture liquid, are separated from the culture liquid, and the lipid is separated and purified.