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公开(公告)号：US20060147991A1
公开(公告)日：2006-07-06
申请号：US11377058
申请日：2006-03-16
申请人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
发明人： Hiroshi Shinoki , Yoshihiko Makino , Yumiko Takeshita , Junichi Yamanouchi , Yukio Sudo , Osamu Seshimoto
CPC分类号： B01J19/0046 , B01J2219/00315 , B01J2219/00497 , B01J2219/00527 , B01J2219/00533 , B01J2219/00576 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00711 , B01J2219/00722 , B82Y30/00 , C07B2200/11 , C07C317/08 , C07H21/00 , C40B40/00
摘要： A reactive solid carrier favorably employable for manufacturing DNA chip is composed of a solid carrier and a plurality of vinylsulfonyl groups or their reactive precursors each of which is fixed onto a surface of the solid carrier by covalent bonding via a linking group, and a method for producing a DNA chip is performed by bringing the reactive solid carrier into contact with nucleotide derivatives or their analogues having a reactive group which is reactive with the vinylsulfonyl group or reactive precursor fixed to the solid carrier.
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公开(公告)号：US07572578B2
公开(公告)日：2009-08-11
申请号：US10568101
申请日：2004-09-08
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
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公开(公告)号：USD634055S1
公开(公告)日：2011-03-08
申请号：US29358302
申请日：2010-03-25
申请人： Kaoru Hokazono , Yumiko Takeshita
设计人： Kaoru Hokazono , Yumiko Takeshita
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公开(公告)号：US20070009893A1
公开(公告)日：2007-01-11
申请号：US10568101
申请日：2004-09-08
CPC分类号： C12N15/1006 , C12N15/1017
摘要： The present invention is a method for isolating and purifying a nucleic acid, where generation of foams is able to be suppressed whereby the isolation and purification of a nucleic acid are easily and efficiently carried out, the method for isolating and purifying a nucleic acid comprising the step of: (1) contacting a sample solution containing nucleic acid to a solid phase to adsorb the nucleic acid onto the solid phase; (2) contacting a washing solution to the solid phase to wash the solid phase in such a state that the nucleic acid is adsorbed; and (3) contacting an elution solution to the solid phase to desorb the nucleic acid, wherein the sample solution containing nucleic acid contains an antifoaming agent.
摘要翻译： 本发明是分离和纯化核酸的方法，其中能够抑制泡沫的产生，从而容易且有效地进行核酸的分离和纯化，分离和纯化包含 步骤：（1）将含有核酸的样品溶液与固相接触以将核酸吸附到固相上; （2）将洗涤溶液与固相接触以在核酸被吸附的状态下洗涤固相; 和（3）使洗脱溶液与固相接触以解吸核酸，其中含有核酸的样品溶液含有消泡剂。
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公开(公告)号：USD634056S1
公开(公告)日：2011-03-08
申请号：US29358303
申请日：2010-03-25
申请人： Kaoru Hokazono , Yumiko Takeshita
设计人： Kaoru Hokazono , Yumiko Takeshita
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公开(公告)号：US20080138884A1
公开(公告)日：2008-06-12
申请号：US11794581
申请日：2006-03-02
CPC分类号： C12N15/1006
摘要： A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of adding at least two enzymes.
摘要翻译： 一种分离和纯化核酸的方法，所述方法包括：（1）使含有核酸的样品溶液与固相接触以将核酸吸附在固相上的步骤; （2）使洗涤液与固相接触以在核酸被吸附在固相上的状态下洗涤固相的步骤; 和（3）使回收溶液与固相接触以从固相解吸核酸的步骤，其中通过包括加入至少两种酶的步骤制备样品溶液。
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公开(公告)号：USD670860S1
公开(公告)日：2012-11-13
申请号：US29395923
申请日：2012-04-23
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公开(公告)号：US20090270603A1
公开(公告)日：2009-10-29
申请号：US11794754
申请日：2006-03-02
CPC分类号： C07H1/00 , C12N15/1006
摘要： A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate.
摘要翻译： 一种分离和纯化核酸的方法，所述方法包括：（1）使含有核酸的样品溶液与固相接触以将核酸吸附在固相上的步骤; （2）使洗涤液与固相接触以在核酸被吸附在固相上的状态下洗涤固相的步骤; 和（3）使回收溶液与固相接触以从固相解吸核酸的步骤，其中通过包括除去沉淀组分的步骤，加入表面活性剂和水溶性的方法制备样品溶液 有机溶剂沉淀到上清液中。
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