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    Fixation of nucleotide derivatives to solid carrier
    2.
    发明授权
    Fixation of nucleotide derivatives to solid carrier 有权
    核苷酸衍生物固定于固体载体

    公开(公告)号：US06664051B1

    公开(公告)日：2003-12-16

    申请号：US09706405

    申请日：2000-11-03

    申请人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    发明人： Hiroshi Shinoki ,  Yukio Sudo ,  Osamu Seshimoto

    IPC分类号： C07H2104

    CPC分类号： C40B40/06 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00677 ,  B82Y30/00 ,  C40B50/06

    摘要： A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

    摘要翻译： 通过以下方法制备DNA芯片或其由连接于固体载体上的核苷酸衍生物组成的类似物的方法：使得在每个末端具有反应性基团的核苷酸衍生物与其中具有反应性基团的固体载体在水相中存在下接触 能够通过核苷酸衍生物的反应性基团和固体载体的反应性基团的重排而产生共价键的转移酶。

    Fluorescent substances
    4.
    发明授权
    Fluorescent substances 失效
    荧光物质

    公开(公告)号：US06642375B2

    公开(公告)日：2003-11-04

    申请号：US09731279

    申请日：2000-12-06

    申请人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    发明人： Hiroko Inomata ,  Hiroshi Shinoki ,  Masayoshi Kojima ,  Yukio Sudo ,  Junji Nishigaki ,  Osamu Seshimoto

    IPC分类号： C07H1904

    CPC分类号： G01N33/582

    摘要： The present invention provides a fluorescent substance which is represented by a formula: A-B-C wherein A is a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue, or A is a residue of avidin or streptavidin; B is a divalent linking group or a single bond; and C is a monovalent group derived from a general formula (I) and binds to B at a reactive group present in R1 or R2: wherein R1 and R2 each independently represent an alkyl group that may be substituted with a reactive group capable of covalently bonding to A-B-; R3, R4, R5, and R6 each independently represent an alkyl group, and R3 and R4, and/or R5 and R6 may bind to each other to form a saturated carbon-ring together with a carbon atom(s) to which they bind; V1, V2, V3, V4, V5, V6, V7, V8, V9 and V10 each independently represent a hydrogen atom or a monovalent substituent, and two adjacent groups thereof may bind to form a ring; L1, L2, and L3 represent a substituted or unsubstituted methine group; each of m, n, s, and t represents 0 or 1, provided that m+n=1 and s+t=1; p represents 1, 2, or 3; M represents a counter ion; and q represents a number required to neutralize the charge of a molecule. The fluorescent substance of the present invention is useful as a labeling substance for nucleic acids, or as a reagent for analyzing biological components such as nucleic acids, proteins or sugars.

    摘要翻译： 本发明提供一种荧光物质，其由下式表示：AB-C，其中A是天然或合成的核苷酸，寡核苷酸，多核苷酸或其衍生物的残基，并与所述残基中的碱基部分的B结合，或A是 抗生物素蛋白或链霉亲和素的残基; B是二价连接基团或单键; 和C是衍生自通式（I）的一价基团，并且在R 1或R 2中存在的反应性基团上与B结合：其中R 1和R 2各自独立地表示烷基， 可以被能够与AB-共价结合的反应性基团取代; R 3，R 4，R 5和R 6各自独立地表示烷基，R 3和R 4和/或R 5和R 6独立地表示烷基， 可以彼此结合形成饱和碳环与它们所结合的碳原子一起; V 1，V 2，V 3，V 4，V 5，V 6，V 7，V 8，V 9和V 10 独立地表示氢原子或一价取代基，其两个相邻基团可以结合形成环; L 1，L 2和L 3表示取代或未取代的次甲基; m，n，s和t中的每一个表示0或1，条件是m + n = 1且s + t = 1; p表示1,2或3; M表示抗衡离子; q表示中和分子电荷所需的数。 本发明的荧光物质可用作核酸的标记物质，或用作分析核酸，蛋白质或糖类等生物成分的试剂。

    Copolymer mordant for photographic dyes and photographic element containing the same
    5.
    发明授权
    Copolymer mordant for photographic dyes and photographic element containing the same 失效
    用于照相染料的共聚物媒染剂和含有它们的摄影元素

    公开(公告)号：US4596756A

    公开(公告)日：1986-06-24

    申请号：US614596

    申请日：1984-05-29

    申请人： Junichi Yamanouchi ,  Yukio Sudo

    发明人： Junichi Yamanouchi ,  Yukio Sudo

    IPC分类号： C08F8/00 ,  C08F8/32 ,  G03C1/835 ,  G03C7/396 ,  G03C8/56 ,  G03C5/54 ,  C08F20/44

    CPC分类号： G03C1/835 ,  G03C7/396 ,  G03C8/56 ,  Y10S430/142

    摘要： A photographic element having at least one layer containing a copolymer mordant having the repeating unit represented by the following general formula (I) ##STR1## wherein A represents a monomer unit (copolymer component) derived from a copolymerizable monomer having at least two ethylenically unsaturated groups; B represents a monomer unit (copolymer unit) derived from a copolymerizable ethylenically unsaturated monomer represented by the following general formula (II) ##STR2## wherein R.sub.5 represents a hydrogen atom or an alkyl group; L represents an alkylene group or an arylene group; and m is 0 or 1; D represents a monomer unit (copolymer component) derived from a copolymerizable ethylenically unsaturated monomer other than the monomer units shown by A and B and the monomer unit having component ratio z; R.sub.1 represents a hydrogen atom or a lower alkyl group; R.sub.2, R.sub.3 and R.sub.4, which may be the same or different, each represents an alkyl group or an aralkyl group, and at least two of R.sub.2, R.sub.3 and R.sub.4 may combine to form a heterocyclic structure with a nitrogen atom; n represents an integer of from 1 to about 12; x.sup..crclbar. represents an anion; w represents 0 to about 10 mol %; x represents from about 5 to about 90 mol %; y represents from 0 to about 80 mol %; and z represents from about 10 to about 95 mol %.

    摘要翻译： 具有至少一层含有具有下述通式（I）所示的重复单元的共聚物媒染剂的照相元件，其中A表示由可共聚单体衍生的单体单元（共聚物组分），所述共聚单体具有至少两个 烯属不饱和基团; B表示由下述通式（II）表示的可共聚烯属不饱和单体衍生的单体单元（共聚单元），其中R 5表示氢原子或烷基; L表示亚烷基或亚芳基; m为0或1; D表示除了由A和B所示的单体单元以外的可共聚烯属不饱和单体和单体单元的组分比率z的单体单元（共聚物组分） R1表示氢原子或低级烷基; R 2，R 3和R 4可以相同或不同，各自表示烷基或芳烷基，R 2，R 3和R 4中的至少两个可以结合形成与氮原子的杂环结构; n表示1〜12的整数， x（ - ）表示阴离子; w表示0〜约10摩尔％ x表示约5至约90mol％; y表示0〜约80摩尔％。 z表示约10至约95mol％。

    Microdevice for performing method of separating and purifying nucleic acid
    6.
    发明申请
    Microdevice for performing method of separating and purifying nucleic acid 审中-公开
    用于分离和纯化核酸的微型设备

    公开(公告)号：US20060068491A1

    公开(公告)日：2006-03-30

    申请号：US11227245

    申请日：2005-09-16

    申请人： Yoshihiko Makino ,  Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe

    发明人： Yoshihiko Makino ,  Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe

    IPC分类号： C12M1/34 ,  C07H21/04

    CPC分类号： B01L3/502753 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2200/0631 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0487

    摘要： A microdevice for performing a method for separating and purifying a nucleic acid, the microdevice comprising: at least one opening; and at least one channel for passing a sample solution, wherein the method comprises: (A) a step of bringing a nucleic acid-containing sample solution into contact with a nucleic acid-adsorbing support having a function of adsorbing a nucleic acid; (B) a step of washing the nucleic acid-adsorbing support with a washing solution in a state of a nucleic acid being adsorbed to the support; and (C) a step of desorbing the nucleic acid from the nucleic acid-adsorbing support by a recovering solution, thereby purifying the nucleic acid; an apparatus for utilizing the microdevice; and a reagent kit for use in the microdevice.

    摘要翻译： 一种用于进行分离和纯化核酸的方法的微型装置，所述微型装置包括：至少一个开口; 和用于通过样品溶液的至少一个通道，其中所述方法包括：（A）使含核酸样品溶液与具有吸附核酸功能的核酸吸附载体接触的步骤; （B）在被吸附在所述载体上的核酸的状态下用洗涤液洗涤所述核酸吸附载体的工序; 和（C）通过回收溶液从核酸吸附载体解吸核酸的步骤，从而纯化核酸; 一种利用微型装置的装置; 和用于微型装置的试剂盒。

    Method of analyzing double stranded DNA
    7.
    发明授权
    Method of analyzing double stranded DNA 失效
    分析双链DNA的方法

    公开(公告)号：US06924105B2

    公开(公告)日：2005-08-02

    申请号：US10039642

    申请日：2001-10-24

    申请人： Yukio Sudo ,  Yoshihiko Makino

    发明人： Yukio Sudo ,  Yoshihiko Makino

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  C12P19/34

    CPC分类号： C12Q1/6816 ,  C12Q1/6804 ,  C12Q1/6825 ,  G01N33/5308 ,  C12Q2565/607 ,  C12Q2565/125 ,  C12Q2563/173 ,  C12Q2522/101

    摘要： The object of the present invention is to provide a method for analyzing double stranded DNA directly without denaturation. According to the present invention, there is provided a method of analyzing double stranded DNA present in an analyte, which comprises the steps of: (1) contacting the analyte with a double stranded DNA recognizing substance immobilized on a support, and (2) measuring double stranded DNA bound to the double stranded DNA recognizing substance.

    摘要翻译： 本发明的目的是提供直接分析双链DNA而不变性的方法。 根据本发明，提供了分析分析物中存在的双链DNA的方法，其包括以下步骤：（1）使分析物与固定在载体上的双链DNA识别物质接触，和（2）测量 与双链DNA识别物质结合的双链DNA。

    Assay chip
    10.
    发明申请
    Assay chip 审中-公开
    测定芯片

    公开(公告)号：US20070178521A1

    公开(公告)日：2007-08-02

    申请号：US10592748

    申请日：2005-03-15

    申请人： Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe ,  Bo Yang

    发明人： Yoshiki Sakaino ,  Yukio Sudo ,  Yoshihiko Abe ,  Bo Yang

    IPC分类号： G01N33/53 ,  C12M3/00

    CPC分类号： B01L3/502753 ,  B01L3/5023 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2400/0409

    摘要： An integrated assay chip for assaying one or more components of a specimen, which comprises: (1) one or more pretreatment elements for pretreating a specimen; (2) one or more multilayer dry assay elements capable of assaying one or more components of the pretreated specimen; and (3) one or more flow channels connecting the pretreatment element and the multilayer dry assay element is provided.

    摘要翻译： 一种用于测定样品的一种或多种组分的综合测定芯片，其包括：（1）用于预处理样品的一种或多种预处理元件; （2）能够测定预处理样品的一种或多种组分的一种或多种多层干测定元件; 和（3）提供连接预处理元件和多层干测定元件的一个或多个流动通道。