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    Method for separating and purifying nucleic acid
    1.
    发明申请
    Method for separating and purifying nucleic acid 审中-公开
    核酸分离纯化方法

    公开(公告)号：US20090270603A1

    公开(公告)日：2009-10-29

    申请号：US11794754

    申请日：2006-03-02

    申请人： Yumiko Takeshita ,  Hiroyuki Komazawa ,  Shinichi Watanabe

    发明人： Yumiko Takeshita ,  Hiroyuki Komazawa ,  Shinichi Watanabe

    IPC分类号： C07H1/00 ,  B01L3/00

    CPC分类号： C07H1/00 ,  C12N15/1006

    摘要： A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of removing a precipitate component, and adding a surfactant and a water-soluble organic solvent to a supernatant solution of the precipitate.

    摘要翻译： 一种分离和纯化核酸的方法，所述方法包括：（1）使含有核酸的样品溶液与固相接触以将核酸吸附在固相上的步骤; （2）使洗涤液与固相接触以在核酸被吸附在固相上的状态下洗涤固相的步骤; 和（3）使回收溶液与固相接触以从固相解吸核酸的步骤，其中通过包括除去沉淀组分的步骤，加入表面活性剂和水溶性的方法制备样品溶液 有机溶剂沉淀到上清液中。

    Method for Separating and Purifying Nucleic Acid
    2.
    发明申请
    Method for Separating and Purifying Nucleic Acid 审中-公开
    分离和纯化核酸的方法

    公开(公告)号：US20080138884A1

    公开(公告)日：2008-06-12

    申请号：US11794581

    申请日：2006-03-02

    申请人： Yumiko Takeshita ,  Hiroyuki Komazawa ,  Shinichi Watanabe

    发明人： Yumiko Takeshita ,  Hiroyuki Komazawa ,  Shinichi Watanabe

    IPC分类号： C07H1/06 ,  C12M1/00

    CPC分类号： C12N15/1006

    摘要： A method for separating and purifying nucleic acid, the method comprising: (1) a step of contacting a sample solution containing nucleic acid with a solid phase to adsorb the nucleic acid on the solid phase; (2) a step of contacting a washing solution with the solid phase to wash the solid phase in a state that the nucleic acid is adsorbed on the solid phase; and (3) a step of contacting a recovering solution with the solid phase to desorb the nucleic acid from the solid phase, wherein the sample solution is prepared by including a step of adding at least two enzymes.

    摘要翻译： 一种分离和纯化核酸的方法，所述方法包括：（1）使含有核酸的样品溶液与固相接触以将核酸吸附在固相上的步骤; （2）使洗涤液与固相接触以在核酸被吸附在固相上的状态下洗涤固相的步骤; 和（3）使回收溶液与固相接触以从固相解吸核酸的步骤，其中通过包括加入至少两种酶的步骤制备样品溶液。

    CM-chitin derivatives and use thereof
    8.
    发明授权
    CM-chitin derivatives and use thereof 失效
    CM几丁质衍生物及其用途

    公开(公告)号：US5298488A

    公开(公告)日：1994-03-29

    申请号：US780790

    申请日：1991-10-23

    申请人： Masayoshi Kojima ,  Hiroyuki Komazawa

    发明人： Masayoshi Kojima ,  Hiroyuki Komazawa

    IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K9/00 ,  C07K14/78 ,  C08B37/08 ,  A61K37/02 ,  C07K5/08 ,  C07K7/06

    CPC分类号： C08B37/003 ,  A61K47/48238 ,  C07K14/78 ,  C07K9/005 ,  A61K38/00

    摘要： A CM-chitin derivative which has, as an essential unit, a cohesive peptide represented by the following general formula (I), bonded to the side chain of CM-chitin, wherein the bond connecting CM-chitin and said peptide is selected from the group consisting of an amido bond, an ester bond, an ether bond and a urethane bond:-[R.sup.1 ]-[CO]-([X]-Arg-Gly-Asp-[Y]).sub.n -[Z]-[R.sup.2 ]- . . . (I)(SEQ ID NO:1)wherein the bracket [ ] means that the corresponding group or residue may be present or absent and if they are present, X and Y each represents an amino acid residue selected from the group consisting of Ser, Gly, Val, Asn and Pro or a peptide residue; Z represents --O-- or --NH--; one of R.sup.1 and R.sup.2 represents a hydrogen atom, or a substituted or unsubstituted alkyl group having 1 to 9 carbon atoms or aryl group having 6 to 9 carbon atoms and the other represents a hydrogen atom, a linear or branched alkylene group having 1 to 9 carbon atoms or an arylene group having 6 to 9 carbon atoms wherein the alkylene and arylene groups may have substituents; and n is an integer ranging from 1 to 5. The derivative or salt thereof is useful as an effective component of compositions for inhibiting adhesion of animal cells or for inhibiting coagulation of blood platelets.

    摘要翻译： 作为必要单元具有与下述通式（I）表示的结合肽键合到CM-几丁质的侧链的CM-壳多糖衍生物，其中连接CM-壳多糖和所述肽​​的键选自 - （R1） - （CO） - （（X）-Arg-Gly-Asp-（Y））n-（Z） - （ R2） - 。 。 。 （I）（SEQ ID NO：1），其中所述支架（）表示相应的基团或残基可以存在或不存在，如果存在，则X和Y各自表示选自Ser， Gly，Val，Asn和Pro或肽残基; Z表示-O-或-NH-; R 1和R 2之一表示氢原子，或取代或未取代的碳原子数1〜9的烷基或碳原子数6〜9的芳基，另外表示氢原子，具有1〜9的直链或支链亚烷基 碳原子或具有6至9个碳原子的亚芳基，其中亚烷基和亚芳基可以具有取代基; n为1〜5的整数。其衍生物或盐可用作抑制动物细胞粘附或抑制血小板凝结的组合物的有效成分。

    Propenamide derivatives containing Arg-Gly-Asp polymers obtained therefrom
    10.
    发明授权
    Propenamide derivatives containing Arg-Gly-Asp polymers obtained therefrom 失效
    含有由其获得的Arg-Gly-Asp聚合物的丙烯酰胺衍生物

    公开(公告)号：US6046289A

    公开(公告)日：2000-04-04

    申请号：US278251

    申请日：1994-07-20

    申请人： Hiroyuki Komazawa ,  Masayoshi Kojima ,  Atsushi Orikasa

    发明人： Hiroyuki Komazawa ,  Masayoshi Kojima ,  Atsushi Orikasa

    IPC分类号： A61K38/00 ,  A61K47/48 ,  C07K5/09 ,  C07K14/75 ,  C07K14/78 ,  C12N5/00 ,  A61K38/02 ,  C07K5/00 ,  C08H1/00

    CPC分类号： C07K5/0817 ,  A61K47/48238 ,  C07K14/75 ,  C07K14/78 ,  C12N5/0068 ,  A61K38/00 ,  C12N2533/30

    摘要： A propenamide derivative of the formula (I) having, as an essential structural unit, an adhesive peptide represented by the formula (II) in the side chain or salts thereof:Formula (I): R.sup.1 R.sup.2 C.dbd.CR.sup.3 --CO--[NH]--Formula (II): --[R.sup.4 ]--[CO]--([X]-Arg-Gly-Asp-[Y]).sub.n -[Z]--[R.sup.5 ]--wherein R.sup.1 and R.sup.2 each represents a hydrogen atom or a carboxyl group and R.sup.3 represents a hydrogen or halogen atom, methyl, ethyl or carboxymethyl group; X and Y each represents an amino acid residue selected from the group consisting of Ser, Gly, Val, Asn and Pro or a peptide residue consisting of two or more of the amino acids; z represents --O-- or --NH--; one of R.sup.4 and R.sup.5 represents a hydrogen atom, and the other represents a linear or branched alkylene group having 1 to 11 carbon atoms or an arylene group having 6 to 11 carbon atoms wherein the alkylene and arylene groups may be substituted with one or more halogen atom, carbonyl, carboxyl, amino, hydroxyl, sulfo, aryl, nitro or cyano group, or unsaturated double or triple bond; n is an integer of from 1 to 5; and [ ] means that each corresponding group or residue therebetween may be present or absent. A copolymer and crosslinked copolymer of the derivative with an anionic or cationic monomer or additional polyfunctional polymer. The use of the compounds for inhibiting adhesion of animal cells, or coagulation.cndot.adhesion of blood platelets, or for cultivating animal cells.

    摘要翻译： 作为必要结构单元的式（I）的丙烯酰胺衍生物，其侧链中的式（II）表示的粘合剂肽或其盐：式（I）：R1R2C = CR3-CO- [NH] - 式（II）：[R 4] - [CO] - （[X] -Arg-Gly-Asp- [Y]）n - [Z] - [R 5] - 其中R 1和R 2各自表示氢原子或 羧基，R 3表示氢或卤素原子，甲基，乙基或羧甲基; X和Y各自表示选自Ser，Gly，Val，Asn和Pro的氨基酸残基或由两个或更多个氨基酸组成的肽残基; z表示-O-或-NH-; R4和R5之一表示氢原子，另一个表示碳原子数1〜11的直链或支链亚烷基或碳原子数6〜11的亚芳基，其中亚烷基和亚芳基可被一个或多个卤素 原子，羰基，羧基，氨基，羟基，磺基，芳基，硝基或氰基，或不饱和双键或三键; n为1〜5的整数， 和[]表示它们之间的每个相应组或残基可以存在或不存在。 衍生物与阴离子或阳离子单体或另外的多官能聚合物的共聚物和交联共聚物。 化合物用于抑制动物细胞粘附，凝血，血小板粘附或培养动物细胞的用途。